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Svetlana V. Kamzolova Emiliya G. Dedyukhina Vladimir A. Samoilenko Julia N. Lunina Irina F. Puntus Ramil L. Allayarov Maria N. Chiglintseva Alexey A. Mironov Igor G. Morgunov 《Applied microbiology and biotechnology》2013,97(20):9133-9144
Production of d S-threo-isocitric acid (ICA) by yeast meets serious difficulties since it is accompanied by a simultaneous production of citric acid (CA) in significant amounts that reduces the yield of desired product. In order to develop an effective process of ICA production, 60 yeast strains of different genera (Candida, Pichia, Saccharomyces, Torulopsis, and Yarrowia) were tested for their ability to produce ICA from rapeseed oil; as a result, wild-type strain Yarrowia lipolytica VKM Y-2373 and its mutant Y. lipolytica 704-UV4-A/NG50 were selected as promising ICA producers. The effects of temperature, pH, aeration, and concentrations of rapeseed oil, iron, and itaconic acid on ICA production by selected strains were studied. Under optimal conditions (pH 6.0; aeration 50–55 %; rapeseed oil concentration of 20–60 gl?1, iron ion concentration of 1.2 mg l?1, and itaconic acid amount of 30 mM), selected strains of Y. lipolytica produced predominantly ICA with a low amount of a by-product, CA. 相似文献