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51.
52.
Photodynamic and non-photodynamic action of several porphyrins on the activity of some heme-enzymes 总被引:1,自引:0,他引:1
S G Afonso S Chinarro J J Mu?oz R E de Salamanca A M Batlle 《Journal of enzyme inhibition》1990,3(4):303-310
The action of porphyrins, uroporphyrin I and III (URO I and URO III), pentacarboxylic porphyrin I (PENTA I), coproporphyrin I and III (COPRO I and COPRO III), protoporphyrin IX (PROTO IX) and mesoporphyrin (MESO), on the activity of human erythrocytes delta-aminolevulinic acid dehydratase, porphobilinogenase, deaminase and uroporphyrinogen decarboxylase in the dark and under UV light was investigated. Both photoinactivation and light-independent inactivation was found in all four enzymes using URO I as sensitizer. URO III had a similar action as URO I on porphobilinogenase and deaminase and PROTO IX exerted equal effect as URO I on delta-aminolevulinic acid dehydratase and uroporphyrinogen decarboxylase. Photodynamic efficiency of the porphyrins was dependent on their molecular structure. Selective photodecomposition of enzymes by URO I, greater specificity of tumor uptake by URO I and enhanced porphyrin synthesis by tumors from delta-aminolevulic acid, with predominant formation of URO I, underline the possibility of using URO I in detection of malignant cells and photodynamic therapy. 相似文献
53.
Changes in the species composition, photosynthesis, calcification and size-fractionated carbon metabolism by natural phytoplankton assemblages were monitored in three mesocosms under different nutrient conditions during May 1993. In the 3 enclosures, the decline of the diatom-dominated assemblages was followed by the development of a bloom of the coccolithoporid Emiliania huxleyi. Highest growth of E. huxleyi was observed in the mesocosm with a high N : P ratio, suggesting this species is a good competitor at low phosphate concentrations. The transition from diatom- to E. huxleyi-dominated assemblages brought about a sharp reduction of the phytoplankton standing stock and carbon-specific photosynthetic rate. The relative contribution of the smaller size fraction to total photosynthesis increased as the succession progressed. Calcification rate and E. huxleyi cell-specified calcite production were highest during the early stages of development of the E. huxleyi bloom. Distinct changes in the patterns of 14C allocation into biomolecules were noticed during the diatom-E. huxleyi succession. The diatom-dominated assemblage showed high relative 14C incorporation into low molecular weight metabolites (LMWM), whereas proteins and, specially, lipids accounted for the largest proportion of carbon incorporation in the E. huxleyi bloom. The patterns of photoassimilated carbon metabolism proved to be strongly dependent on cellular size, as protein relative synthesis was significantly higher in the smaller than in the larger size fraction, irrespective of the nutrient regime and the successional stage. These results are discussed in relation to the ecological and physiological features of small phytoplankton. 相似文献
54.
Antonio Bode Benita Casas Emilio Fernández Emilio Marañón Pablo Serret Manuel Varela 《Hydrobiologia》1996,341(3):225-234
Summary Chlorophyll-a and primary production on the euphotic zone of the N-NW Spanish shelf were studied at 125 stations between 1984 and 1992. Three geographic areas (Cantabrian Sea, Rías Altas and Was Baixas), three bathymetric ranges (20 to 60 m, 60 to 150 m and stations deeper than 200 m), and four oceanographic stages (spring and autumn blooms, summer upwelling, summer stratification and winter mixing) were considered. One of the major sources of variability of chlorophyll and production data was season. Bloom and summer upwelling stages have equivalent mean and maximum values. Average chlorophyll-a concentrations approximately doubled in every step of the increasing productivity sequence: winter mixing — summer stratification — high productivity (upwelling and bloom) stages. Average primary production rates increased only 60% in the described sequence. Mean (± sd) values of chlorophyll-a and primary production rates during the high productivity stages were 59.7 ± 39.5 mg Chl-a m–2 and 86.9 ± 44.0 mg C m–2 h–1, respectively. Significant differences in both chlorophyll and primary production resulted between geographic areas in most stages. Only 27 stations showed the effects of the summer upwelling that affected coastal areas in the Cantabrian Sea and Rías Baixas shelf, but also shelf-break stations in the Rías Altas area. The Rías Baixas area had lower chlorophyll than both the Rías Altas and the Cantabrian Sea areas during spring and autumn blooms, but higher during summer upwelling events. On the contrary, primary production rates were higher in the Rías Baixas area during blooms in spring and autumn. Mid-shelf areas showed the highest chlorophyll concentrations during high productivity stages, probably due to the existence of frontal zones in all geographic areas considered. The estimated phytoplankton growth rates were comparable to those of other coastal upwelling systems, with average values lower than the maximum potential growth rates. Doubling rates for upwelling and stratification stages in the northern and Rías Altas shelf areas were equivalent, despite larger biomass accumulations during upwelling events. Low turnover rates of the existing biomass in the Rías Baixas shelf in upwelling stages suggests that the accumulation of phytoplankton was due mainly to the export from the highly productive rías, while the contribution of in situ production to these accumulations was relatively lower. 相似文献
55.
多胚水稻ApⅢ(双13)的胚胎学观察 总被引:6,自引:0,他引:6
对多胚水稻(Oryza sativa L.)ApⅢ的大量成熟颖果、人工萌发的幼苗和开花后3~5 d 的幼嫩颖果进行的整体解剖和显微制片观察表明:ApⅢ的5000粒成熟颖果中,89.0% 含单胚单苗,8.9% 和1.2%分别含双胚双苗和三胚三苗;700多粒幼嫩颖果中,90.0% ~95.0% 含单胚,5.0% ~7.0% 含双胚。因制片的数目有限,未见到含三胚的;在含单胚和多胚颖果中,胚均位于同一胚囊的珠孔端,未见到胚囊以外存在不定胚。根据上述结果,似可以认为ApⅢ单粒颖果的双胚和三胚是由同一胚囊内的卵细胞和1或2个助细胞受精或不受精发育而来的 相似文献
56.
冬虫夏草模式标本的研究 总被引:4,自引:0,他引:4
对冬虫夏草Cordycepssinensis(Berk.)Sacc.的模式标本进行了形态学和有关文献的研究,井与近邻的同种标本进行了比较;该模式现存英国皇家植物园,邱国标本馆,系1879年由M.J.Berkeley研究的标本。 相似文献
57.
Rossella Serra Gloria Isani Otello Cattani Emilio Carpené 《Biological trace element research》1996,51(1):107-116
Gilthead were fed three diets. Diet A was the control diet, whereas diets B and C were supplemented with 300 and 900 mg Zn/kg,
respectively. Fish fed with diet C, at the end of the experiment, showed the lowest weight. Zinc concentrations presented
the higher values in gills, liver, and kidney. Muscle and brain had the lower mean values and showed a tight control of zinc
levels. These results reinforce the hypothesis that zinc in the CNS should be strictly controlled in order to maintain the
functional role of the metal. Significant differences in tissue zinc concentrations were obtained between fish fed different
amounts of zinc, the metal concentrations being higher in tissues of fish fed diet C. The tissue decrease of zinc, found at
the end of the experiment, may depend on a lower feed consumption or on different zinc requirements during the cold season.
These changes, even if not univocal among the three diets, may be associated with the life cycle of fish. Furthermore, copper
concentrations were little affected by the different concentrations of zinc in the three diets; liver and kidney presented
the highest concentrations; liver showed a significant decrease in copper content at the end of the experiment. We conclude
that: zinc concentrations of the diet may affect the gilthead weights and the tissual metal content; and zinc concentrations
in the diets, depending on the growth rate, may be varied depending on the season. 相似文献
58.
Emilio Fernandez 《Plant Molecular Biology Reporter》1996,14(4):295-295
59.
60.
Prof. Renato Meduri Emilio Campos Lucia Scorolli Caterina De Vinci Giancarlo Pizza Dimitri Viza 《Biotherapy》1996,9(1-3):61-66
Recurrent ocular herpes is an insoluble problem for the clinician. As cellular immunity plays an important role in controlling
herpes relapses, and other studies have shown the efficacy of HSV-specific transfer factor (TF) for the treatment of herpes
patients, an open clinical trial was undertaken in 134 patients (71 keratitis, 29 kerato-uveitis, 34 uveitis) suffering from
recurrent ocular herpetic infections. The mean duration of the treatment was 358 days, and the entire follow-up period 189121
before, and 64062 days after TF treatment. The cell-mediated immune response to the viral antigens, evaluated by the lymphocyte
stimulation test (LST) and the leucocyte migration test (LMT) (P<0.001), was significantly increased by the TF treatment.
The total number of relapses was decreased significantly during/after TF treatment, dropping from 832 before, to 89 after
treatment, whereas the cumulative relapse index (RI) dropped, during the same period, from 13.2 to 4.17 (P<0.0001). No side
effects were observed. It is concluded that patients with relapsing ocular herpes can benefit from treatment with HSV-specific
TF. 相似文献