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91.
Carole Emile 《Option/Bio》2017,28(569-570):26-27
92.
The spider tree of life: phylogeny of Araneae based on target‐gene analyses from an extensive taxon sampling
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Ward C. Wheeler Jonathan A. Coddington Louise M. Crowley Dimitar Dimitrov Pablo A. Goloboff Charles E. Griswold Gustavo Hormiga Lorenzo Prendini Martín J. Ramírez Petra Sierwald Lina Almeida‐Silva Fernando Alvarez‐Padilla Miquel A. Arnedo Ligia R. Benavides Silva Suresh P. Benjamin Jason E. Bond Cristian J. Grismado Emile Hasan Marshal Hedin Matías A. Izquierdo Facundo M. Labarque Joel Ledford Lara Lopardo Wayne P. Maddison Jeremy A. Miller Luis N. Piacentini Norman I. Platnick Daniele Polotow Diana Silva‐Dávila Nikolaj Scharff Tamás Szűts Darrell Ubick Cor J. Vink Hannah M. Wood Junxia Zhang 《Cladistics : the international journal of the Willi Hennig Society》2017,33(6):574-616
93.
Reply to “Clinical and therapeutic implications of BRAF mutation heterogeneity in metastatic melanoma” by Mesbah Ardakani et al.
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94.
The coding region for arginyl-tRNA synthetase from jack bean (Canavalia ensiformis) has been sequenced and cloned into the bacterial expression vector pET32a. Transformation of BL21 cells and induction with IPTG results in the high level expression of the protein fused N-terminally with thioredoxin and bearing a His-tag. A substantial proportion of the enzyme is recovered in the soluble fraction of the cell lysate (10 mg per litre cell culture) and can be isolated with metal-affinity technology. The thioredoxin component and the His-tag portion of the fused protein could be removed with thrombin, resulting in a homogeneous product retaining an N-terminal extension of 3.2 kDa compared to the native arginyl-tRNA synthetase. Both full-length fusion and thrombin-treated products proved to be active in aminoacylation, with similar kinetic parameters. 相似文献
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98.
Emile Levy Daniel Ménard Edgard Delvin Alain Montoudis Jean-François Beaulieu Geneviève Mailhot Nadia Dubé Daniel Sinnett Ernest Seidman Moise Bendayan 《Histochemistry and cell biology》2009,132(3):351-367
Although intestinal (I) and liver (L) fatty acid binding proteins (FABP) have been widely studied, the physiological significance
of the presence of the two FABP forms (I- and L-FABP) in absorptive cells remains unknown as do the differences related to
their distribution along the crypt-villus axis, regional expression, ontogeny and regulation in the human intestine. Our morphological
experiments supported the expression of I- and L-FABP as early as 13 weeks of gestation. Whereas cytoplasmic immunofluorescence
staining of L-FABP was barely detectable in the lower half of the villus and in the crypt epithelial cells, I-FABP was visualized
in epithelial cells of the crypt-villus axis in all intestinal segments until the adult period in which the staining was maximized
in the upper part of the villus. Immunoelectron microscopy revealed more intense labeling of L-FABP compared with I-FABP,
accompanied with a heterogeneous distribution in the cytoplasm, microvilli and basolateral membranes. By western blot analysis,
I- and L-FABP at 15 weeks of gestation appeared predominant in jejunum compared with duodenum, ileum, proximal and distal
colon. Exploration of the maturation aspect documented a rise in L-FABP in adult tissues. Permanent transfections of Caco-2
cells with I-FABP cDNA resulted in decreased lipid export, apolipoprotein (apo) biogenesis and chylomicron secretion. Additionally,
supplementation of Caco-2 with insulin, hydrocortisone and epidermal growth factor differentially modulated the expression
of I- and L-FABP, apo B-48 and microsomal triglyceride transfer protein (MTP), emphasizing that these key proteins do not
exhibit a parallel modulation. Overall, our findings indicate that the two FABPs display differences in localization, regulation
and developmental pattern. 相似文献
99.
Sevcenco AM Pinkse MW Bol E Krijger GC Wolterbeek HT Verhaert PD Hagedoorn PL Hagen WR 《Metallomics : integrated biometal science》2009,1(5):395-402
The tungsten metallome of the hyperthermophilic archaeon Pyrococcus furiosus has been investigated using electroanalytical metal analysis and native-native 2D-PAGE with the radioactive tungsten isotope (187)W (t(1/2) = 23.9 h). P. furiosus cells have an intracellular tungsten concentration of 29 μM, of which ca. 30% appears to be free tungsten, probably in the form of tungstate or polytungstates. The remaining 70% is bound by five different tungsten enzymes: formaldehyde ferredoxin oxidoreductase, aldehyde ferredoxin oxidoreductase, glyceraldehyde-3-phosphate ferredoxin oxidoreductase and the tungsten-containing oxidoreductases WOR4 and WOR5. The membrane proteome of P. furiosus is devoid of tungsten. The differential expression, as measured by the tungsten level, of the five soluble tungsten enzymes when the cells are subjected to a cold-shock shows a strong correlation with previously published DNA microarray analyses. 相似文献
100.
Mahendra Damarla Emile Hasan Adel Boueiz Anne Le Hyun Hae Pae Calypso Montouchet Todd Kolb Tiffany Simms Allen Myers Usamah S. Kayyali Matthias Gaestel Xinqi Peng Sekhar P. Reddy Rachel Damico Paul M. Hassoun 《PloS one》2009,4(2)
Mechanical ventilation, a fundamental therapy for acute lung injury, worsens pulmonary vascular permeability by exacting mechanical stress on various components of the respiratory system causing ventilator associated lung injury. We postulated that MK2 activation via p38 MAP kinase induced HSP25 phosphorylation, in response to mechanical stress, leading to actin stress fiber formation and endothelial barrier dysfunction. We sought to determine the role of p38 MAP kinase and its downstream effector MK2 on HSP25 phosphorylation and actin stress fiber formation in ventilator associated lung injury. Wild type and MK2−/− mice received mechanical ventilation with high (20 ml/kg) or low (7 ml/kg) tidal volumes up to 4 hrs, after which lungs were harvested for immunohistochemistry, immunoblotting and lung permeability assays. High tidal volume mechanical ventilation resulted in significant phosphorylation of p38 MAP kinase, MK2, HSP25, actin polymerization, and an increase in pulmonary vascular permeability in wild type mice as compared to spontaneous breathing or low tidal volume mechanical ventilation. However, pretreatment of wild type mice with specific p38 MAP kinase or MK2 inhibitors abrogated HSP25 phosphorylation and actin polymerization, and protected against increased lung permeability. Finally, MK2−/− mice were unable to phosphorylate HSP25 or increase actin polymerization from baseline, and were resistant to increases in lung permeability in response to HVT MV. Our results suggest that p38 MAP kinase and its downstream effector MK2 mediate lung permeability in ventilator associated lung injury by regulating HSP25 phosphorylation and actin cytoskeletal remodeling. 相似文献