Radish, belonging to the family Brassicaceae, has a self-incompatibility which is controlled by multiple alleles on the
S locus. To employ the self-incompatibility in an F
1 breeding system, identification of
S haplotypes is necessary. Since collection of
S haplotypes and determination of nucleotide sequences of
SLG,
SRK, and
SCR alleles in cultivated radish have been conducted by different groups independently, the same or similar sequences with different
S haplotype names and different sequences with the same
S haplotype names have been registered in public databases, resulting in confusion of
S haplotype names for researchers and breeders. In the present study, we developed
S homozygous lines from radish F
1 hybrid cultivars in Japan and determined the nucleotide sequences of
SCR, the
S domain and the kinase domain of
SRK, and the
SLG of a large number of
S haplotypes. Comparing these sequences with our previously published sequences, the haplotypes were ordered into 23 different
S haplotypes. The sequences of the 23
S haplotypes were compared with
S haplotype sequences registered by different groups, and we suggested a unification of these
S haplotypes. Furthermore, dot-blot hybridization using
SRK allele-specific probes was examined for developing a standard method for
S haplotype identification.
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