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排序方式: 共有568条查询结果,搜索用时 78 毫秒
561.
Emiko Isogai Hiroshi Isogai Toshiyuki Masuzawa Daniele Postic Guy Baranton Yuri Kamewaka Koichi Kimura Takeshi Nishikawa Nobuhiro Fujii Norihisa Ishii Shigeaki Ohno Noboru Yamaguti 《Microbiology and immunology》1996,40(1):13-19
Ticks and blood samples were collected from wild sika deer (Cervus nippon yesoensis) during a hunting season (August to October) of 1991 at a selected location in Hokkaido, Japan. Ixodes persulcatus (adult and nymph) and I. ovatus (adult) were the common ticks on sika deer. Spirochetes were detected in the midgut of the ticks by the indirect peroxidase-conjugated antibody staining method and by dark-field microscopy after cultivation. By the reactive pattern of monoclonal antibodies, isolates were considered to belong to Borrelia garinii or B. japonica. In an antibody test, the percentage of seropositive deer was 69.0%. Most of the adult sika deer were positive for antibodies to the spirochetes. There are significant age-dependency in antibody level and seropositive rate. The surveillance of deer should be valuable in monitoring the transmission risk of B. burgdorferi sensu lato in nature. 相似文献
562.
563.
Takayuki Nagao Kimiaki Saito Emiko Hirayama Kumi Uchikoshi Kiyotaka Koyama Shinsaku Natori Naoko Morisaki Shigeo Iwasaki Tijiro Matsushima 《Mutation research》1989,215(2)
Ptaquiloside, a potent carcinogen of an illudane-type sesquiterpene glycoside isolated from Pteridium aquilium, and its related compounds, hypolosides having the same nucleus isolated from the Pteridaceae, exhited marked mutagenicity in the modified Ames test with Salmonella typhimurian TA98 and A100 using a preincubation at pH 8.5 Illudins M and S, sesquiterpenes of the same illudane type from basidiomycetes, also exhibited mutagenicity. The structural requirements for mutagenicity are discussed. 相似文献
564.
Growth was severely inhibited when tobacco plants were exposed to toxic levels of cadmium (0.2 mM). However, when this treatment
was combined with a high concentration of calcium (30 mM), the Cd-induced damage was strongly alleviated. Under these enhanced
conditions, i.e., 30 mM Ca and 0.2 mM Cd, Ca crystals not only were heavily deposited in the leaves but were also actively
excreted from the trichomes. The X-ray spectrum from our Energy Dispersive analysis revealed that both intra-and extracellular
Ca crystals contained detectable amounts of Cd. Moreover, intracellular Ca deposition in the leaves was stimulated only by
a high Ca concentration (30 mM); moderate levels of Ca (3 mM) or a toxic amount of Cd (0.2 mM) alone resulted in crystal deposition
that was undetectable under a light microscope. In contrast, extracellular crystal formation on the trichomes was stimulated
by toxic Cd treatment but not by high Ca concentrations alone. Finally, Inductively Coupled Plasma Spectroscopy revealed that
a high level of Ca (30 mM) suppressed Cd accumulation while also increasing the endogeneous Ca concentration in the leaves.
These observations imply that the amelioration of Ca against toxic Cd in tobacco plants is a result of not only the inhibition
of Cd uptake, but also the extra-and intracellular sequestration of cadmium via Ca crystallization. 相似文献
565.
Yasuharu Ninomiya Xing Cui Takeshi Yasuda Bing Wang Dong Yu Emiko Sekine-Suzuki Mitsuru Nenoi 《BMB reports》2014,47(10):575-580
In this study, we investigate whether arsenite-induced DNA damage leads to p53-dependent premature senescence using human glioblastoma cells with p53-wild type (U87MG-neo) and p53 deficient (U87MG-E6). A dose dependent relationship between arsenite and reduced cell growth is demonstrated, as well as induced γH2AX foci formation in both U87MG-neo and U87MG-E6 cells at low concentrations of arsenite. Senescence was induced by arsenite with senescence-associated β-galactosidase staining. Dimethyl- and trimethyl-lysine 9 of histone H3 (H3DMK9 and H3TMK9) foci formation was accompanied by p21 accumulation only in U87MG-neo but not in U87MG-E6 cells. This suggests that arsenite induces premature senescence as a result of DNA damage with heterochromatin forming through a p53/p21 dependent pathway. p21 and p53 siRNA consistently decreased H3TMK9 foci formation in U87M G-neo but not in U87MG-E6 cells after arsenite treatment. Taken together, arsenite reduces cell growth independently of p53 and induces premature senescence via p53/p21-dependent pathway following DNA damage. [BMB Reports 2014; 47(10): 575-580] 相似文献
566.
Mauricio Carrillo-Avila Emiko K. Resende Débora K. S. Marques Pedro M. Galetti Jr 《Conservation Genetics》2009,10(5):1597-1599
This paper describes eight polymorphic microsatellite loci of the large migratory catfish Jaú, Zungaro jahu, a threatened species in the Paraguay—Paraná River Basin, which includes the Brazilian wetland Pantanal system. The number
of detected alleles varied from 3 to 10, while observed and expected heterozygosity values ranged from 0.3182 to 0.8333 and
0.3298 to 0.8652, respectively. Cross-amplification on five related species was successfully evaluated. These isolated markers
demonstrated to be very useful for population studies of this fish and related species, consisting in important tools for
evaluations related to the management and conservation of the native populations. 相似文献
567.
568.
Tsuyoshi Hosoya Kiyoshi Hamano Michihiro Sugano Yoko Ogura Emiko Hatano Takakazu Hamada 《Mycoscience》1999,40(6):525-529
Dicephalosterol, a new testosterone 5α-reductase inhibitor, was found from isolates ofDicephalospora rufocornea, a sclerotiniaceous discomycete widely distributed, but not previously cultured. Under SEM observation, the polar appendage
of the ascospores inD. rufocornea was found to be more solid than was hitherto reported. Dicephalosterol was produced by submerged fermentation for 7 d. This
new analogue of testosterone showed an IC50 of 5.7 μg/ml for rat prostatic 5α-reductase, but no antimicrobial activity against bacteria or fungi. 相似文献