全文获取类型
收费全文 | 423篇 |
免费 | 20篇 |
国内免费 | 3篇 |
出版年
2024年 | 1篇 |
2023年 | 16篇 |
2022年 | 35篇 |
2021年 | 46篇 |
2020年 | 24篇 |
2019年 | 23篇 |
2018年 | 27篇 |
2017年 | 8篇 |
2016年 | 17篇 |
2015年 | 21篇 |
2014年 | 17篇 |
2013年 | 30篇 |
2012年 | 28篇 |
2011年 | 33篇 |
2010年 | 10篇 |
2009年 | 11篇 |
2008年 | 11篇 |
2007年 | 12篇 |
2006年 | 8篇 |
2005年 | 8篇 |
2004年 | 11篇 |
2003年 | 8篇 |
2002年 | 10篇 |
2001年 | 6篇 |
2000年 | 2篇 |
1999年 | 1篇 |
1997年 | 3篇 |
1996年 | 1篇 |
1995年 | 5篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1992年 | 2篇 |
1991年 | 2篇 |
1987年 | 1篇 |
1984年 | 1篇 |
1982年 | 1篇 |
1976年 | 1篇 |
1975年 | 1篇 |
排序方式: 共有446条查询结果,搜索用时 15 毫秒
1.
Thirty-eight genera and 81 species of fungi were isolated and identified from 120 samples of 24 kinds of spices collected from different places at Assiut Governorate, Egypt. Predominant genera wereAspergillus (25 species) andPenicillium (7 species) of whichA. flavus, A. niger, A. ochraceus, A. fumigatus, A. flavus var.columnaris, A. terreus, P. chrysogenum andP. corylophilum were the most commonly occurring. 相似文献
2.
Glycerol production by a highly glycerol-producing local isolate (Eurotium amstelodami) and a standard reference isolate (Aspergillus wentii) was markedly enhanced by high saline media. Glycerol concentration depended on the external osmotic. Thus, the highest glycerol
concentration was found in the presence of NaCl, followed by KCl, with considerably lower values for MgCl2 and CaCl2 saline media. With glucose (5–50%) used as a nonionic osmotic, low levels of glycerol were obtained and the main pool of
polyols was mannitol. Glycerol production was gradually increased with the increase of NaCl concentration of cheese whey,
reaching maxima by both organisms when whey was supplemented with 8% NaCl (total of 16% NaCl). The quantity of glycerol produced
byA. wentii was twice higher than that obtained byE. amstelodami on whey treated with 8% NaCl. 相似文献
3.
Five hundred isolates of different xerophilic and non-xerophilic fungi belonging to 10 genera and 74 species were screened
for alditol (sugar alcohol) accumulation. Ninety-two of the isolates failed to grow on a salt medium, most of the isolates
(408) produced alditols; 348,44 and 16 of them produced low, moderate and high levels of alditols, respectively. The high
alditol producers belonged to five species ofAspergillus, six species ofEurotium andFennellia flavipes. Glycerol andd-mannitol were the main constituents of alditol pools of the 16 high alditol producers.d-Arabinitol andmeso-erythritol were also formed but at low concentrations by several of the tested isolates. 相似文献
4.
Mostafa Dalia G. Satti Huda H. Khaleel Eman F. Badi Rehab M. 《Journal of physiology and biochemistry》2020,76(3):393-406
Journal of Physiology and Biochemistry - The increase in osteopontin (OPN) levels after stroke induces neural protection by activating Akt signaling and inhibiting GS3Kβ, iNOS, and NF-κB.... 相似文献
5.
Safaa I. Elewa Mansour Eman Nassar Ibrahim F. Mekawey Amal A. I. 《Russian Journal of Bioorganic Chemistry》2020,46(3):382-392
Russian Journal of Bioorganic Chemistry - 3-(2-Thienyl)-5-aryl-1-thiocarbamoyl-2-pyrazolines were reacted with chloroacetone derivatives and hydrazonyl chloride derivatives in ethanol to afford the... 相似文献
6.
Hanaa Orabi Eman Abdelkhalek Nagwa Sidkey 《Preparative biochemistry & biotechnology》2020,50(4):408-418
AbstractL-glutaminase from bacterial sources has been proven to be effective and economical agents in cancer therapy, food industry and high-value chemicals like threonine. In the present study, a newly isolated bacterial strain was potentially producing extracellular L-glutaminase, it identified as Bacillus subtilis OHEM11 (MK389501) using the 16S rRNA gene. L-glutaminase production optimized and the optimum factors for production under submerged fermentation were at pH 6.5–7.0 and 35?°C after 28?hr using rhamnose and glutamine as carbon and nitrogen sources, respectively, while bagasse was the best inducer for the production under solid-state fermentation. Ethanol precipitation and ion-exchange chromatography using QFF are the purification steps. L-glutaminase was purified to 2-fold with specific activity 89.78?U/mg and its molecular weight about 54.8?kDa with the alkaline property of the enzyme makes it clear having carcinostatic property; maximum enzyme activity at pH 8.2 and 40?°C and retained about 90% activity for 1?hr. The cytotoxicity effect of L-glutaminase indicated a significant safety on Vero cells with high anticancer activity against NFS-60, HepG-2, and MCF-7 cancer cell lines. The outcomes demonstrated that L-glutaminase could be applied in many biotechnological applications such as pharmaceutical and food processing. 相似文献
7.
Kanika Narula Eman Elagamey Magdi A. E. Abdellatef Arunima Sinha Sudip Ghosh Niranjan Chakraborty Subhra Chakraborty 《The Plant journal : for cell and molecular biology》2020,103(2):561-583
Pathogen‐/microbe‐associated molecular patterns (PAMPs/MAMPs) initiate complex defense responses by reorganizing the biomolecular dynamics of the host cellular machinery. The extracellular matrix (ECM) acts as a physical scaffold that prevents recognition and entry of phytopathogens, while guard cells perceive and integrate signals metabolically. Although chitosan is a known MAMP implicated in plant defense, the precise mechanism of chitosan‐triggered immunity (CTI) remains unknown. Here, we show how chitosan imparts immunity against fungal disease. Morpho‐histological examination revealed stomatal closure accompanied by reductions in stomatal conductance and transpiration rate as early responses in chitosan‐treated seedlings upon vascular fusariosis. Electron microscopy and Raman spectroscopy showed ECM fortification leading to oligosaccharide signaling, as documented by increased galactose, pectin and associated secondary metabolites. Multiomics approach using quantitative ECM proteomics and metabolomics identified 325 chitosan‐triggered immune‐responsive proteins (CTIRPs), notably novel ECM structural proteins, LYM2 and receptor‐like kinases, and 65 chitosan‐triggered immune‐responsive metabolites (CTIRMs), including sugars, sugar alcohols, fatty alcohols, organic and amino acids. Identified proteins and metabolites are linked to reactive oxygen species (ROS) production, stomatal movement, root nodule development and root architecture coupled with oligosaccharide signaling that leads to Fusarium resistance. The cumulative data demonstrate that ROS, NO and eATP govern CTI, in addition to induction of PR proteins, CAZymes and PAL activities, besides accumulation of phenolic compounds downstream of CTI. The immune‐related correlation network identified functional hubs in the CTI pathway. Altogether, these shifts led to the discovery of chitosan‐responsive networks that cause significant ECM and guard cell remodeling, and translate ECM cues into cell fate decisions during fusariosis. 相似文献
8.
A correlation between BCL-2 modifying factor,p53 and livin gene expressions in cancer colon patients
Eman AE. Badr Mohamed FA. Assar Abdel Monem A. Eltorgoman Azza Zaghlol Labeeb Gehad A. Breaka Enas A. Elkhouly 《Biochemistry and Biophysics Reports》2020
Accumulating evidence has revealed that livin gene and BCL-2 modifying factor (BMF) gene are closely associated with the initiation and progression of colon carcinoma by activating or suppressing multiple malignant processes. Those genes that can detect colon - cancer are a promising approach for cancer screening and diagnosis. This study aimed to evaluate correlation between livin, BMF and p53 genes expression in colon cancer tissues of patients included in the study, and their relationship with clinicopathological features and survival outcome in those patients. In this study, 50 pathologically diagnosed early cancer colon patients included and their tissue biopsy with 50 matched adjacent normal tissue, and 50 adenoma tissue specimens were analyzed for livin gene and BMF gene expressions using real time PCR. The relationship of those genes expressions with clinicopathological features, tumor markers, Time to Progression and overall survival for those patients were correlated in cancer colon group. In this study, there was a significant a reciprocal relationship between over expression of livin gene and down regulation of BMF and p53 genes in colon cancer cells. Livin mRNA was significantly higher, while BMF and p53 mRNA were significantly lower in colorectal cancer tissue compared to benign and normal colon tissue specimens (P < 0.001), however, this finding was absent between colon adenomas and normal mucosa. There was a significant association between up regulation of livin and down regulation of BMF and p53 expressions with more aggressive tumor (advanced TNM stage), rapid progression with metastasis and decreased overall survival in cancer colon patients, hence these genes can serve as significant prognostic markers of poor outcome in colon cancer patients. This work highlights the role of livin, BMF and p53 genes in colorectal tumorigenesis and the applicability of using those genes as a diagnostic and prognostic markers in patients with colon carcinoma and as a good target for cancer colon treatment in the future. 相似文献
9.
Teer Eman Joseph Danzil E. Dominick Leanne Glashoff Richard H. Essop M. Faadiel 《中国病毒学》2021,36(5):1133-1143
Virologica Sinica - Although antiretroviral treatment lowers the burden of human immunodeficiency virus (HIV)-related disease, it does not always result in immunological recovery. This manifests as... 相似文献
10.
Pavithra A. Honnaiah Shankarappa Sridhara Pradeep Gopakkali Nandini Ramesh Eman A. Mahmoud Shaimaa A.M. Abdelmohsen Fatema H. Alkallas Diaa O. El-Ansary Hosam O. Elansary 《Saudi Journal of Biological Sciences》2021,28(6):3453-3460
Crop growth largely depends on radiation. Radiation is the main impetus for photosynthesis and movement of photosynthates from source to sink. Therefore, identification of the optimum sowing windows and suitable cultivars for efficient utilization of radiation is of prime importance. A field study was conducted in red clay soil during 2014 and 2015 Kharif season and the treatments consisted of three genotypes and three sowing windows by using randomized complete block design with three replications. The effect of genotypes and sowing windows was found significant with respect to number of trifoliate leaves, leaf area ratio, dry matter production, grain numbers, pod length, test weight, grain yield, and stover yield of guar during 2014 as compared to 2015 sown crop. Statistically significant plant height, number of trifoliate leaves, number of branches, leaf area ratio, absolute growth rate, leaf area index, dry matter, grain number, pod length, grain yield, stover yield and a higher cumulative radiation interception were recorded with 15th August sown crop as compared to other sowing windows. The plant height, number of trifoliate leaves, number of branches, leaf area ratio, absolute growth rate, leaf area index, dry matter, grain number, pod length, grain yield, stover yield and maximum cumulative interception of radiation were significant with RGC-1003 as compared to RGC-936 and HG-365. It is observed that the incident PAR to dry matter accumulation conversion efficiency was varied with cultivars and different sowing windows which ranges from 0.74 g MJ−1 to 0.79 g MJ−1. 相似文献