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排序方式: 共有390条查询结果,搜索用时 31 毫秒
51.
Eman Mohammed Halawani 《Saudi Journal of Biological Sciences》2021,28(12):7379-7389
The global spread of antimicrobial-resistant infectious diseases and cancer are the most widespread public health issue and has led to high mortality rates. This study aims to evaluate and verify the antibacterial and antitumor activities of Shaoka and Manuka honey against pathogenic bacteria, human hepatocarcinoma (HepG2) and breast cancer (MCF-7) cell lines. Shaoka hone was analyzed using HPLC, UV–vis, and GC/MC, while antibacterial activity was measured by agar diffusion, broth microdilution methods, and Transmission Electron Microscopy (TEM). Antitumor activity was investigated morphologically and by MTT assay. According to the presented data of HPLC analysis, Shaoka honey was generally richer in polyphenolic components, the antibacterial activity showed that Shaoka honey is equivalent or relatively more active than Manuka honey against a broad spectrum of multi-drug-resistant bacteria. It inhibited the growth of ESBL Escherichia coli in the absence or presence of catalase enzyme with a concentration approximately 8.5%–7.3% equivalent to phenol, which supported the highest level of non-peroxide-dependent activity. The minimum bactericidal concentrations (MBCs) ranged between 5.0% and 15.0% honey (w/v). TEM observation revealed distorted cell morphology, cytoplasmic shrinkage, and cell wall destruction of treated bacteria. The selected honey exerted cytotoxicity on both cancer cell lines, inhibiting cell proliferation rate and viability percent in HepG2 and MCF-7 cancer cells, by different degrees depending on the honey quality, Shaoka honey competed Manuka inhibitory effects against both cancer cells. The obtained data confirmed the potential for use of Saudi Shaoka honey as a remedy, this well introduces a new honey template as medical-grade honey for treating infectious disease and cancer. 相似文献
52.
Samia Hussein Hala Mosaad Hayam E. Rashed Shimaa Ahmed Ahmed Ragab Eman I. Ismail 《Molecular biology reports》2017,44(4):365-377
This study aimed to assess the expression of S100A4, Twist and E-cadherin (mRNA and protein) in urothelial bladder cancer, investigate the correlation between them and evaluate their association with the clinicopathological features of the disease. The study included 54 patients diagnosed as urothelial bladder cancer of different stages and grades. The expression levels of S100A4, Twist and E-cadherin (mRNA and protein) in tissue samples were determined by quantitative RT-PCR and immunohistochemistry. The expression of S100A4 and Twist was significantly upregulated while E- cadherin was significantly downregulated in urothelial bladder cancer tissues compared to the adjacent surrounding normal bladder tissues at both mRNA and protein levels (p?<?0.001). Expression levels of S100A4 and Twist were significantly higher in recurrent tumor than in non-recurrent tumors (p?<?0.001) while the expression level of E-cadherin was significantly lower in recurrent tumors than in non-recurrent tumors at both mRNA and protein levels (p?<?0.001). There was a significant positive correlation between S100A4 and Twist expressions (r?=?0.875, p?<?0.001) while significant negative correlations were found between E- cadherin and S100A4 expressions(r=- 0.803, p?<?0.001) and between E-cadherin and Twist (r?=??0.809, p?<?0.001). Up-regulation of S100A4 and Twist and down-regulation of E-cadherin in urothelial bladder cancer tissues compared to adjacent normal tissues were observed. There was a significant negative correlation between S100A4 and E- cadherin and between E- cadherin and Twist expression. However, there was a significant positive correlation between S100A4 and Twist expressions. Furthermore, the alterations in the gene expression were associated with disease stage and grade. 相似文献
53.
The objective was to determine the prevalence of iodine deficiency among hypothyroid patients and the effect of dietary goitrogens
on indices of iodine and thyroid status. This is a case-control study of 106 subjects who were recruited from King Abdulaziz
University Hospital, Jeddah. Blood and urine were collected for serum thyroid hormones, thyroid autoantibodies, thyroglobulin
(Tg) and urinary iodine concentration (UIC). Dietary iodine and goitrogenic food intake were assessed by questionnaire. Using
World Health Organization (WHO) cutoff values for UIC, both controls and cases were iodine deficient (85% and 83%, respectively).
Furthermore, dietary iodine was deficient in 23% of controls and 36% of cases. In cases, there was a positive association
between UIC levels and serum thyroid stimulating hormone (r = 0.405, p < 0.01) and a negative association with serum fT4 (r = −0.358, p < 0.01). Serum Tg antibody titers were also positively associated with dietary iodine (r = 0.328, p < 0.05). Patients with elevated serum autoantibodies had lower UIC and dietary iodine than those with normal serum autoantibodies.
UIC was associated with dietary goitrogens including turnip (r = 0.280, p < 0.05) and pine (r = 0.289, p < 0.05) among cases. Iodine deficiency is common and the consumption of dietary goitrogens is high among euthyroid and hypothyroid
subjects living in Jeddah. 相似文献
54.
Saleh I. Alqasoumi Areej M. Al-Taweel Ahmed M. Alafeefy Mostafa M. Hamed Eman Noaman Mostafa M. Ghorab 《Bioorganic & medicinal chemistry letters》2009,19(24):6939-6942
A large number of antimitotic drugs, derived from natural sources or chemically synthesized, have been identified and shown to interfere with the tubulin system. Inhibition of tubulin polymerization is among the important targets useful in the cancer therapy.The present work reports the synthesis of some novel quinoline derivatives bearing a trimethoxyphenyl moiety. The trimethoxybenzene moiety has been reported to be crucial to obtain relevant cytotoxic and antitubulin responses. All the newly synthesized compounds were evaluated for their in vitro anticancer activity. Several compounds showed interesting cytotoxic activities compared to the used reference drug. 相似文献
55.
Mostafa M. El-Sheekh Abdel-mohsen S. Ismail Mostafa A. El-Abd Eman M. Hegazy Ahmed I. El-Diwany 《International biodeterioration & biodegradation》2009,63(1):12-18
The production of a notable and highly effective pectinase by the local fungal strain Aspergillus carneus NRC1 utilizing the abundant Egyptian orange peels and pulps (OPP) scraps excluded in the orange juice and canning industry was achieved in 5-day submerged fermentation (SMF) cultures, at temperature and pH ranges of 30–55 °C and 5.0–5.5, respectively. Fresh or thawed OPP (6%, w/v) were the most preferable sole carbon source. Pectinase activity was dramatically stimulated by ammonium sulphate as the sole nitrogen source, and at the same time strongly inhibited the production of the other tested enzymes, i.e., cellulases and hemicellulases. The lyophilized enzyme preparation was free from any ochra or aflatoxins. The optimum conditions of this methodology including enzyme and substrate (citrus pectin) concentrations were 40 mg ml?1 and 7% (w/v), respectively, with pH and temperature of 4.0 and 55 °C, respectively. 相似文献
56.
Omar S. Desouky Nabila S. Selim Eman M. El-Bakrawy Seham M. El-Marakby 《Cell biochemistry and biophysics》2009,55(1):45-53
Thalassemia is the world’s most common hereditary disease; therefore, more interest has been devoted for the development of
the screening procedure of this disease. In β-thalassemia major, the subject of the current study, impaired biosynthesis of
beta-globin leads to accumulation of unpaired alpha-globin chain. The objective of the present study, was to examine many
of the biophysical properties of β-thalassemia major red blood cells (RBCs) and to study the possibility of use of any of them as a preliminary screening tool
for β-thalassemia. The percentage of normal hemolysis, osmotic fragility test, turbidity test, rheological properties, and dielectric
properties, were studied in 20 regularly blood transfused thalassemia major patients who were under chelation therapy and
their status were compared with those of 10 healthy subjects. There was an increase in the percentage of hemolysis for β-thalassemia by 114.6% compared to the normal RBCs. The fragility curve for β-thalassemia RBCs showed a shift toward lower NaCl concentration compared to the normal curve. The average osmotic fragility
(H
50: the NaCl concentration producing 50% homolysis) for β-thalassemia was found to be 3.21 ± 0.67 g/l, whereas for normal RBCs it was 5.5 ± 0.31 g/l. The turbidity curve of the β-thalassemic RBCs showed a shift toward higher detergent concentration of the normal curve, with higher value for the average
membrane solubilization (S
50). The viscosity value of whole blood β-thalassemia was found to be 3.916 ± 0.56 cp whereas for normal blood was 2.516 ± 0.36 cp. The relative permittivity, dielectric
loss, and AC conductivity of RBCs decreased significantly compared to normal samples. This could be attributed to the loss
of the insulating properties of the membrane and loss of its surface charge of thalassemic RBCs. As can be noticed, several
factors showed clear difference between thalassemic and normal blood samples. Some of these parameters could be measured immediately
after sample withdrawal and require short time to perform the measurements. This offers the advantages of being effective,
low cost, and fast techniques, therefore, we suggest that these techniques could be applied for β-thalassemia major screening purposes. 相似文献
57.
Mona M. Al-Dosary Abdullah M. Al-Bekairi Eman B. Moursy 《Saudi Journal of Biological Sciences》2010,17(2):177-183
The harvested eggs of Rhynchophorus ferrugineus are ovo-cylindrical shaped, averaged 1.09 mm in length and 0.43 mm in width, with ratio of L\W 4.42. The chorionic layer of electron dense material is seen covering the exochorion structure of the eggs. The egg main body chorion exhibits a polygonal pattern and architecture surface of the egg is supported by a system of irregular interconnecting grooves. The micropylar apparatus of the eggs of the Red Palm Weevil, R. ferrugineus is described in the present study for the first time. Two micropylar openings are found closed to the center of the posterior wide pole of the egg. Each micropylar opening presents a single small orifice and its surrounding chorion is porous and densely set with tiny projections allowing the spermatozoa to penetrate the egg. Respiratory aeropyles are distributed on the borders of reticulations in the area chorionic surface of egg capsule. The hatching region is detected on the anterior part at the opposite side of the egg. Changes in the appearance and shape of R. ferrugineus eggs as well as the incidence of embryonic development are observed. 相似文献
58.
Amin KM Ismail MM Noaman E Soliman DH Ammar YA 《Bioorganic & medicinal chemistry》2006,14(20):6917-6923
Hypoxic cells which are common feature of solid tumors are resistant to both anticancer drugs and radiation therapy. Thus, the identification of drugs with the selective toxicity toward hypoxic cells is an important target in anticancer chemotherapy. Tirapazamine has been shown to be an efficient and selective cytotoxin after bioreductive activation in hypoxic cells which is thought to be due to the presence of the 1,4-di-N-oxide. A new series of quinoxaline 1,4-di-N-oxides and fused quinoxaline di-N-oxides were synthesized and evaluated for hypoxic-cytotoxic activity on EAC cell line. Compound 10a was the most potent cytotoxin IC(50) 0.9 microg/mL, potency 75 microg/mL, and was approximately 15 times more selective cytotoxin (HCR>111) than 3-aminoquinoxaline-2-carbonitrile which has been used as a standard (HCR>7.5). Compounds 4 and 3a,b were more selective than the standard. In addition, antitumor activity against Hepg2 (liver) and U251 (brain) human cell lines was evaluated, compounds 9c and 8a were the most active against Hepg2 with IC(50) values 1.9 and 2.9 microg/mL, respectively, however, all the tested compounds were nontoxic against U251 cell line. 相似文献
59.
Hassan Reda Hassan Elsayed Mohammad El-Nablaway Basma Adel Khattab Rania N. Sherif Wagdi Fawzy Elkashef Asim Mohammed Abdalla Eman Mohammad El Nashar Mostafa Mohammed Abd-Elmonem Randa El-Gamal 《The journal of histochemistry and cytochemistry》2021,69(9):575
Non-alcoholic steatohepatitis (NASH) is a worldwide health problem. Alternate-day fasting (ADF), although thought to be aggressive, has proven safety and efficacy. We aimed to evaluate the effect of short-term ADF against already established high-fat-fructose (HFF)-induced NASH, independent of the amount of calorie intake, and to study the effect of ADF on lipogenesis, apoptosis, and hepatic inflammation. Male Sprague Dawley rats were divided into two groups: (1) negative control and (2) NASH group fed on HFF for 9 weeks, and then randomized into two subgroups of either HFF alone or with ADF protocol for 3 weeks. The ADF could improve HFF-related elevation in serum lactate dehydrogenase and could decrease the mRNA expression of lipogenesis genes; acetyl CoA carboxylase, peroxisome proliferator-activated receptor γ, and peroxisome proliferator-activated receptor α; apoptotic genes caspase-3, p53, and inflammatory cyclo-oxygenase 2; and immunohistochemical staining for their proteins in liver with upregulation of LC3 and downregulation of P62 immunoexpression. Moreover, ADF ameliorated HFF-induced steatosis, inflammation, ballooning, and fibrosis through hematoxylin and eosin, Oil Red O, and Sirius Red staining, confirmed by morphometric analysis, without significant weight loss. Significant correlation of morphometric parameters with levels of gene expression was found. These findings suggest ADF to be a safe effective therapeutic agent in the management of NASH 相似文献