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61.
Els Lommelen Tom Wenseleers Christine A. Johnson Falko P. Drijfhout Johan Billen Bruno Gobin 《Journal of Insect Behavior》2010,23(3):236-249
Approximately 150 ant species are facultatively or obligately queenless whereby mated workers assume the role of the queen.
In many of these species a reproductive dominance hierarchy is established by way of aggressive interactions. Top-ranking
workers, which are typically the most fecund, acquire a characteristic cuticular hydrocarbon profile. We studied the temporal
dynamics of this chemical change and associated interplay with observed aggressive interactions in an experimentally orphaned
colony of the facultatively queenless ant Gnamptogenys striatula. Our observations and chemical analyses demonstrate that chemical fertility signals played a major role in the establishment
of a dominance hierarchy and aggression settled dominance relationships only when ants had identical hydrocarbon profiles.
Moreover, individuals with a higher potential fertility, in this experiment reflected in a higher ovariole number, are shown
to have a better chance of becoming a reproductive. 相似文献
62.
Adeno-associated viruses (AAV) are widely spread throughout the human population, yet no pathology has been associated with infection. This fact, together with the availability of simple molecular techniques to alter the packaged viral genome, has made AAV a serious contender in the search for an ideal gene therapy delivery vehicle. However, our understanding of the intriguing features of this virus is far from exhausted and it is likely that the mechanisms underlying the viral lifestyle will reveal possible novel strategies that can be employed in future clinical approaches. One such aspect is the unique approach AAV has evolved in order to establish latency. In the absence of a cellular milieu that will support productive viral replication, wild-type AAV can integrate its genome site specifically into a locus on human chromosome 19 (termed AAVS1), where it resides without apparent effects on the host cell until cellular conditions are changed by outside influences, such as adenovirus super-infection, which will lead to the rescue of the viral genome and productive replication. This article will introduce the biology of AAV, the unique viral strategy of targeted genome integration and address relevant questions within the context of attempts to establish therapeutic approaches that will utilize targeted gene addition to the human genome. 相似文献
63.
Els A. Baerends 《American anthropologist》2000,102(2):356-357
Inagina: The Last House of Iron. 1998. 52 minutes, color. video by Eric Huyesecom and Bernard Augustoni in French and English. For more information contact Documentary Educational Resources, 101 Morse Street, Watertown, MA 02172, Tel. (617) 926-0491, Fax (617) 926-9519; http://anthropologie.unige.ch/inagina . 相似文献
64.
Fouquaert E Hanton SL Brandizzi F Peumans WJ Van Damme EJ 《Plant & cell physiology》2007,48(7):1010-1021
The Galanthus nivalis agglutinin (GNA) is synthesized as a preproprotein. To corroborate the role of the different targeting peptides in the topogenesis of GNA and related proteins, different constructs were made whereby both the complete original GNA gene and different truncated sequences were coupled to the enhanced green fluorescent protein (EGFP). In addition, a GNA ortholog from rice that lacks the signal peptide and C-terminal propeptide sequence was fused to EGFP. These fusion constructs were expressed in tobacco BY-2 cells and their localization analyzed by confocal fluorescence microscopy. We observed that the processed preproprotein of GNA was directed towards the vacuolar compartment, whereas both the truncated forms of GNA corresponding to the mature lectin polypeptide and the rice ortholog of GNA were located in the nucleus and the cytoplasm. It can be concluded, therefore, that removal of the C-terminal propeptide and the signal peptide is sufficient to change the subcellular targeting of a normally vacuolar protein to the nuclear/cytoplasmic compartment of the BY-2 cells. These findings support the proposed hypothesis that cytoplasmic/nuclear GNA-like proteins and their vacuolar homologs are evolutionarily related and that the classical GNA-related lectins might have evolved from cytoplasmic orthologs through an evolutionary event involving the insertion of a signal peptide and a C-terminal propeptide. 相似文献
65.
A novel family of lectins evolutionarily related to class V chitinases: an example of neofunctionalization in legumes
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A lectin has been identified in black locust (Robinia pseudoacacia) bark that shares approximately 50% sequence identity with plant class V chitinases but is essentially devoid of chitinase activity. Specificity studies indicated that the black locust chitinase-related agglutinin (RobpsCRA) preferentially binds to high-mannose N-glycans comprising the proximal pentasaccharide core structure. Closely related orthologs of RobpsCRA could be identified in the legumes Glycine max, Medicago truncatula, and Lotus japonicus but in no other plant species, suggesting that this novel lectin family most probably evolved in an ancient legume species or possibly an earlier ancestor. This identification of RobpsCRA not only illustrates neofunctionalization in plants, but also provides firm evidence that plants are capable of developing a sugar-binding domain from an existing structural scaffold with a different activity and accordingly sheds new light on the molecular evolution of plant lectins. 相似文献
66.
Human-specific expression of Siglec-6 in the placenta 总被引:3,自引:0,他引:3
67.
Impact of live cell imaging on coated vesicle research 总被引:1,自引:0,他引:1
The role of membrane traffic is to transfer cargo between distinct subcellular compartments. Each individual trafficking event involves the creation, transport and fusion of vesicular and tubular carriers that are formed and regulated via cytoplasmic coat protein complexes. The dynamic nature of this process is therefore highly suitable for studying using live cell imaging techniques. Although these approaches have raised further questions for the field, they have also been instrumental in providing essential new information, in particular relating to the morphology of transport carriers and the exchange kinetics of coat proteins and their regulators on membranes. Here, we present an overview of live cell-imaging experiments that have been used in the study of coated-vesicle transport, and provide specific examples of their impact on our understanding of coat function. 相似文献
68.
Farrokh Modabber Pierre A Buffet Els Torreele Geneviéve Milon Simon L Croft 《Kinetoplastid biology and disease》2007,6(1):3
Background
A meeting was organized by Drugs for Neglected Diseases initiative (DNDi) and the Institute Pasteur (IP), Paris, to review the treatment for all forms of cutaneous leishmaniasis (CL) and to propose a strategy for the development of new efficacious and affordable treatments. 相似文献69.
Integral and associated lysosomal membrane proteins 总被引:2,自引:0,他引:2
Schröder B Wrocklage C Pan C Jäger R Kösters B Schäfer H Elsässer HP Mann M Hasilik A 《Traffic (Copenhagen, Denmark)》2007,8(12):1676-1686
We searched for novel proteins in lysosomal membranes, tentatively participating in molecular transport across the membrane and/or in interactions with other compartments. In membranes purified from placental lysosomes, we identified 58 proteins, known to reside at least partially in the lysosomal membrane. These included 17 polypeptides comprising or associated with the vacuolar adenosine triphosphatase. We report on additional 86 proteins that were significantly enriched in the lysosomal membrane fraction. Among these, 12 novel proteins of unknown functions were found. Three were orthologues of rat proteins that have been identified in tritosomes by Bagshaw RD et al. (A proteomic analysis of lysosomal integral membrane proteins reveals the diverse composition of the organelle. Mol Cell Proteomics 2005;4:133-143). Here, the proteins encoded by LOC201931 (FLJ38482) and LOC51622 (C7orf28A) were expressed with an appended fluorescent tag in HeLa cells and found to be present in lysosomal organelles. Among the lysosomally enriched proteins, also 16 enzymes and transporters were detected that had not been assigned to lysosomal membranes previously. Finally, our results identified a particular set of proteins with known functions in signaling and targeting to be at least partially associated with lysosomes. 相似文献
70.
Multi‐scale habitat modification by coexisting ecosystem engineers drives spatial separation of macrobenthic functional groups
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Serena Donadi Tjisse van der Heide Theunis Piersma Els M. van der Zee Ellen J. Weerman Johan van de Koppel Han Olff Clare Devine Udhi E. Hernawan Myra Boers Linda Planthof Britas Klemens Eriksson 《Oikos》2015,124(11):1502-1510
By changing habitat conditions, ecosystem engineers increase niche diversity and have profound effects on the distribution and abundances of other organisms. Although many ecosystems contain several engineering species, it is still unclear how the coexistence of multiple engineers affects the physical habitat and the structure of the community on a landscape scale. Here, we investigated through a large‐scale field manipulation how three coexisting engineers on intertidal flats (cockles Cerastoderma edule; lugworms Arenicola marina; blue mussels Mytilus edulis) influence the functional composition of the local macrobenthic community and what the consequences are at the landscape level. By using biological trait analysis (BTA), we show that on the local scale biogenic changes in sediment accumulation and organic matter content translated into specific shifts in the distribution of functional traits within the community. At a landscape scale, the co‐occurrence of multiple ecosystem engineers resulted in the spatial separation of different functional groups, i.e. different functional groups dominated unique complementary habitats. Our results emphasize the role of co‐occurring multiple engineers in shaping natural communities, thus contributing to a better knowledge of community assembly rules. This understanding can profitably be used to improve ecosystem‐based management and conservation actions. 相似文献