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41.
Tropospheric ozone (O3) is a harmful air pollutant that can negatively impact plant growth and development. Current O3 concentrations ([O3]) decrease forest productivity and crop yields and future [O3] will likely increase if current emission rates continue. However, the specific effects of elevated [O3] on reproductive development, a critical stage in the plant's lifecycle, have not been quantitatively reviewed. Data from 128 peer‐reviewed articles published from 1968 to 2010 describing the effects of O3 on reproductive growth and development were analysed using meta‐analytic techniques. Studies were categorized based on experimental conditions, photosynthetic type, lifecycle, growth habit and flowering class. Current ambient [O3] significantly decreased seed number (?16%), fruit number (?9%) and fruit weight (?22%) compared to charcoal‐filtered air. In addition, pollen germination and tube growth were decreased by elevated [O3] compared to charcoal‐filtered air. Relative to ambient air, fumigation with [O3] between 70 and 100 ppb decreased yield by 27% and individual seed weight by 18%. Reproductive development of both C3 and C4 plants was sensitive to elevated [O3], and lifecycle, flowering class and reproductive growth habit did not significantly affect a plant's response to elevated [O3] for many components of reproductive development. However, elevated [O3] decreased fruit weight and fruit number significantly in indeterminate plants, and had no effect on these parameters in determinate plants. While gaps in knowledge remain about the effects of O3 on plants with different growth habits, reproductive strategies and photosynthetic types, the evidence strongly suggests that detrimental effects of O3 on reproductive growth and development are compromising current crop yields and the fitness of native plant species. 相似文献
42.
43.
Nonlinear amplification of gene expression of master regulators is essential for cellular differentiation. Here we investigated determinants that control the kinetics of the genetic switching process from the vegetative state (B-state) to the competent state (K-state) of Bacillus subtilis, explicitly including the switching window which controls the probability for competence initiation in a cell population. For individual cells, we found that after initiation of switching, the levels of the master regulator [ComK](t) increased with sigmoid shape and saturation occurred at two distinct levels of [ComK]. We analyzed the switching kinetics into the state with highest [ComK] and found saturation after a switching period of length 1.4 ± 0.3 h. The duration of the switching period was robust against variations in the gene regulatory network of the master regulator, whereas the saturation levels showed large variations between individual isogenic cells. We developed a nonlinear dynamics model, taking into account low-number stochastic effects. The model quantitatively describes the probability and timescale of switching at the single cell level and explains why the ComK level in the K-state is highly sensitive to extrinsic parameter variations. Furthermore, the model predicts a transition from stochastic to deterministic switching at increased production rates of ComK in agreement with experimental data. 相似文献
44.
45.
Leisner JJ Vogensen FK Kollmann J Aideh B Vandamme P Vancanneyt M Ingmer H 《Systematic and applied microbiology》2008,31(2):151-156
Chitin is a polysaccharide widely distributed in nature. Among 115 strains from 29 species of lactic acid bacteria only strains belonging to Carnobacterium divergens and Carnobacterium maltaromaticum hydrolyzed alpha-chitin. This activity was not affected by temperature (10 degrees C versus 30 degrees C) and in most cases not subject to glucose catabolite repression. 相似文献
46.
Gene sequences with high homologies to the two genes fatA and fatD, associated with the pJM1 virulence plasmid-encoded iron-sequestering system (anguibactin) in Vibrio anguillarum serogroup O1 strains, were found in a range of serogroup O2a strains and one NT 4 strain that either contained no plasmids or only small-sized plasmids. None of these strains contained the angR gene responsible for regulation of the anguibactin synthesis. DNA sequences of presumed fatA and fatD fragments from one O2a strain and one NT4 strain were identical to each other. The fatA sequence of these strains included 35 nucleotide substitutions and 7 amino acid substitutions out of 555 nucleotides when compared with the fatA sequence of the O1 strain 76775. The fatD sequence included 10 nucleotide substitutions and 1 amino acid substitution out of 543 nucleotides when compared with 76775. The fact that the majority of nucleotide substitutes in the fatA and fatD fragments did not result in amino acid substitutes suggest that these sequences may to some degree be preserved by selective pressure. The functional role of these genes in the O2a serogroup is most likely different from their role in the pJM1 system. 相似文献
47.
Zilbauer M Dorrell N Elmi A Lindley KJ Schüller S Jones HE Klein NJ Núnez G Wren BW Bajaj-Elliott M 《Cellular microbiology》2007,9(10):2404-2416
Campylobacter jejuni is the foremost cause of bacterial-induced diarrhoeal disease worldwide. Although it is well established that C. jejuni infection of intestinal epithelia triggers host innate immune responses, the mechanism(s) involved remain poorly defined. Innate immunity can be initiated by families of structurally related pattern-recognition receptors (PRRs) that recognize specific microbial signature motifs. Here, we demonstrated maximal induction of epithelial innate responses during infection with live C. jejuni cells. In contrast when intestinal epithelial cells (IECs) were exposed to paraformaldehyde-fixed bacteria, host responses were minimal and a marked reduction in the number of intracellular bacteria was noted in parallel. These findings suggested a role for intracellular host-C. jejuni interactions in eliciting early innate immunity. We therefore investigated the potential involvement of a family of intracellular, cytoplasmic PRRs, the nucleotide-binding oligomerization domain (NOD) proteins in C. jejuni recognition. We identified NOD1, but not NOD2, as a major PRR for C. jejuni in IEC. We also found that targeting intestinal epithelial NOD1 with small interfering RNA resulted in an increase in number of intracellular C. jejuni, thus highlighting a critical role for NOD1-mediated antimicrobial defence mechanism(s) in combating this infection at the gastrointestinal mucosal surface. 相似文献
48.
Adrian Elmi Lars-?Ke Idahl Per-Erik Sandstr?m Janove Sehlin 《Experimental diabetes research》2000,1(2):155-164
To determine the effect of D-glucose on the β-cell
Na+/K+ pump, 86Rb+ influx was studied in isolated,
-cell-rich islets of Umeå-ob/ob mice in the absence
or presence of lmM ouabain. D-glucose (20 mM)
stimulated the ouabain-sensitive portion of 86Rb+
influx by 65%, whereas the ouabain-resistant
portion was inhibited by 48%. The Na+/K+ ATPase
activity in homogenates of islets of Umeå-ob/ob
mice or normal mice was determined to search for
direct effects of D-glucose. Thus, ouabain-sensitive
ATP hydrolysis in islet homogenates was measured
in the presence of different D-glucose concentrations.
No effect of D-glucose (3–20 mM)
was observed in either ob/ob or normal islets at
the optimal Na+/K+ ratio for the enzyme (135 mM
Na+ and 20 mM K+). Neither D-glucose (3–20 mM)
nor L-glucose or 3-O-methyl-D-glucose (20 mM)
affected the enzyme activity at a high Na+/K+
ratio (175 mM Na+ and 0.7mM K+). Diphenylhydantoin
(150 μM) decreased the enzyme activity at
optimal Na+/K+ ratio, whereas 50 μM of the drug
had no effect. The results suggest that D-glucose
induces a net stimulation the Na+/K+ pump of β-cells in intact islets and that D-glucose does
not exert any direct effect on the Na+/K+ ATPase activity. 相似文献
49.
The bile salt sodium taurocholate induces Campylobacter jejuni outer membrane vesicle production and increases OMV‐associated proteolytic activity 下载免费PDF全文
50.
J. J. Leisner J. Haaber 《Proceedings. Biological sciences / The Royal Society》2012,279(1747):4513-4521
Bacteriocins are bacterial proteinaceous toxins with bacteriostatic or bacteriocidal activity towards other bacteria. The current theory on their biological role concerns especially colicins, with underlying social interactions described as an example of spite. This leads to a rock–paper–scissors game between colicin producers and sensitive and resistant variants. The generality of this type of selection mechanism has previously been challenged with lactic acid bacterial (LAB) bacteriocins as an example. In the natural environment of LAB, batch cultures are the norm opposed to the natural habitats of Escherichia coli where continuous cultures are prevailing. This implies that fitness for LAB, to a large degree, is related to survival rates (bottleneck situations) rather than to growth rates. We suggest that the biological role of LAB bacteriocins is to enhance survival in the stationary growth phase by securing a supply of nutrients from lysed target cells. Thus, this social interaction is an example of selfishness rather than of spite. Specifically, it fits into an ecological model known as intraguild predation (IGP), which is a combination of competition and predation where the predator (LAB bacteriocin producer) and prey (bacteriocin susceptible bacteria) share similar and often limited resources. We hypothesize that IGP may be a common phenomenon promoting microbial production of antagonistic compounds. 相似文献