全文获取类型
收费全文 | 570篇 |
免费 | 55篇 |
专业分类
625篇 |
出版年
2023年 | 2篇 |
2022年 | 4篇 |
2021年 | 7篇 |
2020年 | 5篇 |
2019年 | 8篇 |
2018年 | 5篇 |
2017年 | 8篇 |
2016年 | 12篇 |
2015年 | 34篇 |
2014年 | 30篇 |
2013年 | 28篇 |
2012年 | 42篇 |
2011年 | 39篇 |
2010年 | 22篇 |
2009年 | 23篇 |
2008年 | 29篇 |
2007年 | 38篇 |
2006年 | 29篇 |
2005年 | 31篇 |
2004年 | 35篇 |
2003年 | 30篇 |
2002年 | 37篇 |
2001年 | 6篇 |
2000年 | 7篇 |
1999年 | 5篇 |
1998年 | 6篇 |
1997年 | 3篇 |
1996年 | 6篇 |
1995年 | 3篇 |
1993年 | 8篇 |
1992年 | 5篇 |
1991年 | 5篇 |
1990年 | 4篇 |
1989年 | 6篇 |
1988年 | 7篇 |
1987年 | 6篇 |
1985年 | 4篇 |
1984年 | 6篇 |
1983年 | 5篇 |
1982年 | 4篇 |
1981年 | 6篇 |
1980年 | 5篇 |
1979年 | 4篇 |
1977年 | 3篇 |
1974年 | 1篇 |
1973年 | 1篇 |
1971年 | 1篇 |
1968年 | 1篇 |
1964年 | 1篇 |
1962年 | 3篇 |
排序方式: 共有625条查询结果,搜索用时 0 毫秒
1.
2.
Plant hormone immunoassay 总被引:1,自引:0,他引:1
Elmar W. Weiler 《Physiologia plantarum》1982,54(2):230-234
3.
4.
Patterns and controls of the variability of radiation use efficiency and primary productivity across terrestrial ecosystems 总被引:2,自引:0,他引:2
Martín F. Garbulsky Josep Peñuelas Dario Papale Jonas Ardö Michael L. Goulden Gerard Kiely Andrew D. Richardson Eyal Rotenberg Elmar M. Veenendaal Iolanda Filella 《Global Ecology and Biogeography》2010,19(2):253-267
Aim The controls of gross radiation use efficiency (RUE), the ratio between gross primary productivity (GPP) and the radiation intercepted by terrestrial vegetation, and its spatial and temporal variation are not yet fully understood. Our objectives were to analyse and synthesize the spatial variability of GPP and the spatial and temporal variability of RUE and its climatic controls for a wide range of vegetation types. Location A global range of sites from tundra to rain forest. Methods We analysed a global dataset on photosynthetic uptake and climatic variables from 35 eddy covariance (EC) flux sites spanning between 100 and 2200 mm mean annual rainfall and between ?13 and 26°C mean annual temperature. RUE was calculated from the data provided by EC flux sites and remote sensing (MODIS). Results Rainfall and actual evapotranspiration (AET) positively influenced the spatial variation of annual GPP, whereas temperature only influenced the GPP of forests. Annual and maximum RUE were also positively controlled primarily by annual rainfall. The main control parameters of the growth season variation of gross RUE varied for each ecosystem type. Overall, the ratio between actual and potential evapotranspiration and a surrogate for the energy balance explained a greater proportion of the seasonal variation of RUE than the vapour pressure deficit (VPD), AET and precipitation. Temperature was important for determining the intra‐annual variability of the RUE at the coldest energy‐limited sites. Main conclusions Our analysis supports the idea that the annual functioning of vegetation that is adapted to its local environment is more constrained by water availability than by temperature. The spatial variability of annual and maximum RUE can be largely explained by annual precipitation, more than by vegetation type. The intra‐annual variation of RUE was mainly linked to the energy balance and water availability along the climatic gradient. Furthermore, we showed that intra‐annual variation of gross RUE is only weakly influenced by VPD and temperature, contrary to what is frequently assumed. Our results provide a better understanding of the spatial and temporal controls of the RUE and thus could lead to a better estimation of ecosystem carbon fixation and better modelling. 相似文献
5.
Determination of phytohormones in phloem exudate from tree species by radioimmunoassay 总被引:3,自引:0,他引:3
A sensitive and specific radioimmunoassay was used to determine quantitatively four of the most important phytohormones in the phloem exudate from 14 different tree species of 8 genera. For cytokinins and indole-3-acetic acid (IAA) we found higher concentrations than those reported previously for other species. The gibberellin values were of the same order of magnitude as in earlier analyses (with different methods) of tree phloem exudates, but lower than the ones reported for Ricinus. Free abscisic acid (ABA) was found in tree phloem exudates in similar concentrations as before in Yucca or palm phloem exudate, but at considerably lower ones than reported for Ricinus and in Lupinus phloem exudate.Abbreviations IAA
indole-3-acetic acid
- ABA
abscisic acid
- GA
gibberellic acid 相似文献
6.
The centrosome linker component C‐Nap1 (encoded by CEP250) anchors filaments to centrioles that provide centrosome cohesion by connecting the two centrosomes of an interphase cell into a single microtubule organizing unit. The role of the centrosome linker during development of an animal remains enigmatic. Here, we show that male CEP250 −/− mice are sterile because sperm production is abolished. Premature centrosome separation means that germ stem cells in CEP250 −/− mice fail to establish an E‐cadherin polarity mark and are unable to maintain the older mother centrosome on the basal site of the seminiferous tubules. This failure prompts premature stem cell differentiation in expense of germ stem cell expansion. The concomitant induction of apoptosis triggers the complete depletion of germ stem cells and consequently infertility. Our study reveals a role for centrosome cohesion in asymmetric cell division, stem cell maintenance, and fertility. 相似文献
7.
Oliver SN Lunn JE Urbanczyk-Wochniak E Lytovchenko A van Dongen JT Faix B Schmälzlin E Fernie AR Geigenberger P 《Plant physiology》2008,148(3):1640-1654
The aim of this work was to investigate the effect of decreased cytosolic pyruvate kinase (PKc) on potato (Solanum tuberosum) tuber metabolism. Transgenic potato plants with strongly reduced levels of PKc were generated by RNA interference gene silencing under the control of a tuber-specific promoter. Metabolite profiling showed that decreased PKc activity led to a decrease in the levels of pyruvate and some other organic acids involved in the tricarboxylic acid cycle. Flux analysis showed that this was accompanied by changes in carbon partitioning, with carbon flux being diverted from glycolysis toward starch synthesis. However, this metabolic shift was relatively small and hence did not result in enhanced starch levels in the tubers. Although total respiration rates and the ATP to ADP ratio were largely unchanged, transgenic tubers showed a strong decrease in the levels of alternative oxidase (AOX) protein and a corresponding decrease in the capacity of the alternative pathway of respiration. External feeding of pyruvate to tuber tissue or isolated mitochondria resulted in activation of the AOX pathway, both in the wild type and the PKc transgenic lines, providing direct evidence for the regulation of AOX by changes in pyruvate levels. Overall, these results provide evidence for a crucial role of PKc in the regulation of pyruvate levels as well as the level of the AOX in heterotrophic plant tissue, and furthermore reveal that these parameters are interlinked in vivo. 相似文献
8.
Wanlu Zhang Azqa Khan Jlenia Vitale Annett Neuner Kerstin Rink Christian Lüchtenborg Britta Brügger Thomas H. Sllner Elmar Schiebel 《Open biology》2021,11(11)
The integral membrane protein Apq12 is an important nuclear envelope (NE)/endoplasmic reticulum (ER) modulator that cooperates with the nuclear pore complex (NPC) biogenesis factors Brl1 and Brr6. How Apq12 executes these functions is unknown. Here, we identified a short amphipathic α-helix (AαH) in Apq12 that links the two transmembrane domains in the perinuclear space and has liposome-binding properties. Cells expressing an APQ12 (apq12-ah) version in which AαH is disrupted show NPC biogenesis and NE integrity defects, without impacting Apq12-ah topology or NE/ER localization. Overexpression of APQ12 but not apq12-ah triggers striking over-proliferation of the outer nuclear membrane (ONM)/ER and promotes accumulation of phosphatidic acid (PA) at the NE. Apq12 and Apq12-ah both associate with NPC biogenesis intermediates and removal of AαH increases both Brl1 levels and the interaction between Brl1 and Brr6. We conclude that the short amphipathic α-helix of Apq12 regulates the function of Brl1 and Brr6 and promotes PA accumulation at the NE possibly during NPC biogenesis. 相似文献
9.
Beltramini M Colangelo N Giomi F Bubacco L Di Muro P Hellmann N Jaenicke E Decker H 《The FEBS journal》2005,272(8):2060-2075
The hemocyanin of the tiger shrimp, Penaeus monodon, was investigated with respect to stability and oxygen binding. While hexamers occur as a major component, dodecamers and traces of higher aggregates are also found. Both the hexamers and dodecamers were found to be extremely stable against dissociation at high pH, independently of the presence of calcium ions, in contrast to the known crustacean hemocyanins. This could be caused by only a few additional noncovalent interactions between amino acids located at the subunit-subunit interfaces. Based on X-ray structures and sequence alignments of related hemocyanins, the particular amino acids are identified. At all pH values, the p50 and Bohr coefficients of the hexamers are twice as high as those of dodecamers. While the oxygen binding of hexamers from crustaceans can normally be described by a simple two-state model, an additional conformational state is needed to describe the oxygen-binding behaviour of Penaeus monodon hemocyanin within the pH range of 7.0 to 8.5. The dodecamers bind oxygen according to the nested Monod-Whyman-Changeaux (MWC) model, as observed for the same aggregation states of other hemocyanins. The oxygen-binding properties of both the hexameric and dodecameric hemocyanins guarantee an efficient supply of the animal with oxygen, with respect to the ratio between their concentrations. It seems that under normoxic conditions, hexamers play the major role. Under hypoxic conditions, the hexamers are expected not to be completely loaded with oxygen. Here, the dodecamers are supposed to be responsible for the oxygen supply. 相似文献
10.
Niklas J Priesnitz C Rose T Sandig V Heinzle E 《Applied microbiology and biotechnology》2012,93(4):1637-1650
Metabolic responses of the new neuronal human cell line AGE1.HN to various substrate levels were analyzed in this study showing
that reduced substrate and especially pyruvate load improves metabolic efficiency, leading to improved growth and α1-antitrypsin (A1AT) production. The adaptation of the metabolism to different pyruvate and glutamine concentrations was analyzed
in detail using a full factorial design. The most important finding was an increasingly inefficient use of substrates as well
as the reduction of cell proliferation with increasing pyruvate concentrations in the medium. Cultivations with different
feeding profiles showed that the highest viable cell density and A1AT concentration (167% of batch) was reached in the culture
with the lowest glucose level and without pyruvate feeding. Analysis of metabolic fluxes in the differently fed cultures revealed
a more efficient metabolic phenotype in the cultures without pyruvate feeding. The measured in vitro enzyme activities of
the selected enzymes involved in pyruvate metabolism were lower in AGE1.HN compared with CHO cells, which might explain the
higher sensitivity and different adaptation of AGE1.HN to increased pyruvate concentrations. The results indicate on the one
hand that increasing the connectivity between glycolysis and the TCA cycle might improve substrate use and, finally, the production
of A1AT. On the other hand, a better balanced substrate uptake promises a reduction of energy spilling which is increased
with increasing substrate levels in this cell line. Overall, the results of this study provide important insights into the
regulation of primary metabolism and into the adaptation of AGE1.HN to different substrate levels, providing guidance for
further optimization of production cell lines and applied process conditions. 相似文献