Mechanistic insight into nuclear receptor‐mediated regulation of bile acid metabolism and lipid homeostasis by grape seed procyanidin extract (GSPE)

Dietary procyanidins have emerged as important bioactive components that regulate various metabolic pathways to maintain homeostasis. Grape seed procyanidin extract (GSPE), in particular, has demonstrated regulatory effects on bile acid and lipid metabolism in vivo. While numerous studies in rodent models have shown the potent hypolipidemic action of grape seed extracts, human studies have shown inconsistent results. This review will focus on the molecular mechanisms underlying the hypolipidemic actions of GSPE identified to date, specifically highlighting the effects exerted via nuclear receptors. Such evidence may provide avenues for future research in human subjects with GSPE as a therapeutic treatment for the prevention and amelioration of the metabolic syndrome and cardiovascular disease.     

Exact age determination in laboratory and field-caught Drosophila

A method to prepare and count daily growth layers in internal thoracic muscle attachments is described. Growth layers have been observed in five species in the laboratory and in five field-caught species—including Drosophila melanogaster and D. pseudoobscura. The growth layers do not vary with the genetic make up of individuals within a species, and are comparable between individuals of different species. However, a daily temperature cycle is required for the production of strong layers in laboratory stocks.     

Inter-annual variation in the gonad cycle of the mackerel icefish

Spawning by Antarctic fish is generally considered to be seasonal and restricted to a brief period during the autumn and winter. Arising from this it has been assumed that the gonadal maturation cycle is also closely associated with the time of year. The gonad maturation cycle of the mackerel icefish Champsocephalus gunnari was investigated using data collected during research surveys and from sampling the commercial fishery. Spawning appears to occur at the same time each year, but the timing of gonadal development is subject to a considerable inter-annual variation. The implications of this variation are discussed with respect to feeding conditions.     

Diel patterns of predation and fledging at nests of four species of grassland songbirds

Although it is common for nestlings to exhibit a strong bias for fledging in the morning, the mechanisms underlying this behavior are not well understood. Avoiding predation risk has been proposed as a likely mechanism by a number of researchers. We used video surveillance records from studies of grassland birds nesting in North Dakota, Minnesota, and Wisconsin to determine the diel pattern of nest predation and fledging patterns of four ground‐nesting obligate grassland passerines (Grasshopper Sparrow (Ammodramus savannarum), Savannah Sparrow (Passerculus sandwichensis), Bobolink (Dolichonyx oryzivorus), and Eastern Meadowlark (Sturnella magna)). We used the nest predation pattern as a surrogate for predation activity to test whether nestlings minimized predation risk by avoiding fledging when predation activity was high and preferentially fledging when predation risk was low. Predation activity was significantly lower starting 3 hr before sunrise and ending 3 hr after sunrise, followed by a transition to a period of significantly higher activity lasting for 4 hr, before declining to an average activity level for the rest of the diel period. There was little evidence that the four grassland bird species avoided fledging during the high‐risk period and Savannah Sparrow fledged at higher rates during that period. All four species had hours during the low‐risk period where they fledged at higher rates, but only Grasshopper Sparrow fledged preferentially during that period. Bobolink and Eastern Meadowlark had multiple hours with high fledging rates throughout the daytime period, resulting in no relationship between probability of fledging and predation risk. Given the species variability in fledging pattern seen in our study, it is unlikely that there is a universal response to any driver that affects time of fledging. Further study is needed to understand the complex interplay between species ecology and drivers such as physiology, energetics, and predation in affecting grassland bird fledging behavior.