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81.
T J Wenzel M A van den Berg W Visser J A van den Berg H Y Steensma 《European journal of biochemistry》1992,209(2):697-705
Pyruvate dehydrogenase mutants of Saccharomyces cerevisiae were isolated by disruption of the PDA1 gene. To this end, the PDA1 gene encoding the E1 alpha subunit of the pyruvate dehydrogenase complex was replaced by the dominant Tn5ble marker. Disruption of the PDA1 gene abolished production of the E1 alpha subunit and pyruvate dehydrogenase activity. Two additional phenotypes were observed in the Pdh-mutants: (a) a reduced growth rate in glucose medium which was partially complemented by the amino acid leucine; (b) an increase in formation of petites which lack mitochondrial DNA [rho0], during growth on glucose. Both phenotypes were shown to be a result of inactivation of the PDA1 gene. Explanations for these phenotypes are discussed. 相似文献
82.
Rob Elles 《Molecular biotechnology》1997,8(2):95-104
Clinical molecular genetics has recently become recognized as a diagnostic discipline. This article covers the evolution,
structure, and possible forward development of clinical molecular genetics. Topics covered include general test categories,
introducing new tests, laboratory facilities, staffing and training, and overview of quality issues. 相似文献
83.
Anne-Marie Zeeman Marijke A.H. Luttik Jack T. Pronk Johannes P. van Dijken H.Yde Steensma 《FEMS microbiology letters》1999,177(1):23-28
A Kluyveromyces lactis mutant with a disruption in the KlPDA1 gene, encoding the E1 alpha subunit of the pyruvate dehydrogenase complex, exhibited a four-fold reduced specific growth rate on glucose in minimal medium. Growth of the Klpda1 mutant on glucose in complex medium was not affected. Its growth on defined media could be restored by adding amino acids that require mitochondrial acetyl-CoA for their biosynthesis as nitrogen sources. This, together with the observation that low-concentrations of L-carnitine also restored growth on glucose, indicates that the slow-growth phenotype of the Klpda1 mutant is due to a limited capacity of the mitochondria for import of cytosolic acetyl-CoA. 相似文献