Germination and establishment of seedlings in different phases of theCalluna life cycle in a Scottish heathland

Previous authors have suggested that cyclical succession takes place inCalluna-dominated heathland, and is determined by the different growth phases of the dominant. Characteristic species are said to invade at different stages of the life cycle ofCalluna, particularly in the initial and pioneer phases and in the degenerate phase. In the last phase, gaps are formed due to separation and dying of the oldest frame branches.This paper aims to analyze in which microhabitat and in which phase of theCalluna life cycle seedling emergence and establishment is possible.More seedlings appear in pioneerCalluna than in matureCalluna stands in experimentally manipulated micro-sites. In pioneerCalluna establishment is affected only by application of litter, in matureCalluna by increased illumination. The differences between treatments disappeared after three years, but not the difference in establishment between stands.Seedling emergence from species sown experimentally was different in the successive phases ofCalluna. Highest emergence was in the pioneer stand.In a seedling survey also most seedlings appeared and established in the pioneer phase. The number of recently germinated seeds was high in all phases. However, seedling survival was very low, except in the pioneer stands. This study does not of itself show evidence for cyclical succession inCalluna-dominated heath vegetation. Colonization of gaps by seedlings ofCalluna in mature or degenerate phases is possible, but must be an infrequent occurrence.Nomenclauture follows Clapham, Tutin & Warburg (1962).We wish to thank Dr G. R. Miller for stimulating discussions during the start of the experiments. E. de Hullu wishes to thank the Botany Department in Aberdeen for their hospitality during the research period.     

Hearing sensitivity and amplitude coding in bats are differentially shaped by echolocation calls and social calls

Differences in auditory perception between species are influenced by phylogenetic origin and the perceptual challenges imposed by the natural environment, such as detecting prey- or predator-generated sounds and communication signals. Bats are well suited for comparative studies on auditory perception since they predominantly rely on echolocation to perceive the world, while their social calls and most environmental sounds have low frequencies. We tested if hearing sensitivity and stimulus level coding in bats differ between high and low-frequency ranges by measuring auditory brainstem responses (ABRs) of 86 bats belonging to 11 species. In most species, auditory sensitivity was equally good at both high- and low-frequency ranges, while amplitude was more finely coded for higher frequency ranges. Additionally, we conducted a phylogenetic comparative analysis by combining our ABR data with published data on 27 species. Species-specific peaks in hearing sensitivity correlated with peak frequencies of echolocation calls and pup isolation calls, suggesting that changes in hearing sensitivity evolved in response to frequency changes of echolocation and social calls. Overall, our study provides the most comprehensive comparative assessment of bat hearing capacities to date and highlights the evolutionary pressures acting on their sensory perception.     

Exome Sequencing Identifies Rare Deleterious Mutations in DNA Repair Genes FANCC and BLM as Potential Breast Cancer Susceptibility Alleles

Despite intensive efforts using linkage and candidate gene approaches, the genetic etiology for the majority of families with a multi-generational breast cancer predisposition is unknown. In this study, we used whole-exome sequencing of thirty-three individuals from 15 breast cancer families to identify potential predisposing genes. Our analysis identified families with heterozygous, deleterious mutations in the DNA repair genes FANCC and BLM, which are responsible for the autosomal recessive disorders Fanconi Anemia and Bloom syndrome. In total, screening of all exons in these genes in 438 breast cancer families identified three with truncating mutations in FANCC and two with truncating mutations in BLM. Additional screening of FANCC mutation hotspot exons identified one pathogenic mutation among an additional 957 breast cancer families. Importantly, none of the deleterious mutations were identified among 464 healthy controls and are not reported in the 1,000 Genomes data. Given the rarity of Fanconi Anemia and Bloom syndrome disorders among Caucasian populations, the finding of multiple deleterious mutations in these critical DNA repair genes among high-risk breast cancer families is intriguing and suggestive of a predisposing role. Our data demonstrate the utility of intra-family exome-sequencing approaches to uncover cancer predisposition genes, but highlight the major challenge of definitively validating candidates where the incidence of sporadic disease is high, germline mutations are not fully penetrant, and individual predisposition genes may only account for a tiny proportion of breast cancer families.     

The isolation of mitochondria from dipteran flight muscle

Procedures for the isolation of mitochondria from dipteran flight muscle have been investigated in an attempt to determine the extent and to identify the causes of deterioration associated with isolation. In the light of the results obtained isolation procedures have been improved by minimising mechanical damage, avoiding the development of anoxic conditions, and by the use of an isolation medium of a more physiological nature, containing the potassium salt of an organic anion as the principal osmoeffector, phosphate as the principal buffer, and low concentrations of free Mg²⁺. The oxidative capacity of mitochondria isolated by the improved method is adequate to support the in vivo requirements of the flight system.     

Transportin Mediates Nuclear Entry of DNA in Vertebrate Systems

Delivery of DNA to the cell nucleus is an essential step in many types of viral infection, transfection, gene transfer by the plant pathogen Agrobacterium tumefaciens and in strategies for gene therapy. Thus, the mechanism by which DNA crosses the nuclear pore complex (NPC) is of great interest. Using nuclei reconstituted in vitro in Xenopus egg extracts, we previously studied DNA passage through the nuclear pores using a single-molecule approach based on optical tweezers. Fluorescently labeled DNA molecules were also seen to accumulate within nuclei. Here we find that this import of DNA relies on a soluble protein receptor of the importin family. To identify this receptor, we used different pathway-specific cargoes in competition studies as well as pathway-specific dominant negative inhibitors derived from the nucleoporin Nup153. We found that inhibition of the receptor transportin suppresses DNA import. In contrast, inhibition of importin β has little effect on the nuclear accumulation of DNA. The dependence on transportin was fully confirmed in assays using permeabilized HeLa cells and a mammalian cell extract. We conclude that the nuclear import of DNA observed in these different vertebrate systems is largely mediated by the receptor transportin. We further report that histones, a known cargo of transportin, can act as an adaptor for the binding of transportin to DNA.     

Increase of Fusarium Rot in Stored Citrus Fruit

In recent years the incidence of Fusarium spp. isolated from Stem End Rot (SER) and Internal Core Rot (ICR) of grapefruit and oranges has increased markedly. Fusarium spp. were isolated from apparently healthy green buttons and from the tissue underneath the button of healthy fruits. Of all the SER- and ICR-rotted fruit, the incidence of Fusarium spp. alone and of Fusarium spp. with Alternaria citri was between 75 and 100%. Fusarium was also found to be the single causal agent of ICR of oranges and grapefruit. Species of Fusarium isolated from Stem End Rot and Internal Core Rot of citrus fruit were F. oxysporum, F. moniliforme and recently also F. culmorum.     

Researchers Keep Rejecting Grandmother Cells after Running the Wrong Experiments: The Issue Is How Familiar Stimuli Are Identified

There is widespread agreement in neuroscience and psychology that the visual system identifies objects and faces based on a pattern of activation over many neurons, each neuron being involved in representing many different categories. The hypothesis that the visual system includes finely tuned neurons for specific objects or faces for the sake of identification, so‐called “grandmother cells”, is widely rejected. Here it is argued that the rejection of grandmother cells is premature. Grandmother cells constitute a hypothesis of how familiar visual categories are identified, but the primary evidence against this hypothesis comes from studies that have failed to observe neurons that selectively respond to unfamiliar stimuli. These findings are reviewed and it is shown that they are irrelevant. Neuroscientists need to better understand existing models of face and object identification that include grandmother cells and then compare the selectivity of these units with single neurons responding to stimuli that can be identified.     

Teeth outside the mouth: The evolution and development of shark denticles

Vertebrate skin appendages are incredibly diverse. This diversity, which includes structures such as scales, feathers, and hair, likely evolved from a shared anatomical placode, suggesting broad conservation of the early development of these organs. Some of the earliest known skin appendages are dentine and enamel-rich tooth-like structures, collectively known as odontodes. These appendages evolved over 450 million years ago. Elasmobranchs (sharks, skates, and rays) have retained these ancient skin appendages in the form of both dermal denticles (scales) and oral teeth. Despite our knowledge of denticle function in adult sharks, our understanding of their development and morphogenesis is less advanced. Even though denticles in sharks appear structurally similar to oral teeth, there has been limited data directly comparing the molecular development of these distinct elements. Here, we chart the development of denticles in the embryonic small-spotted catshark (Scyliorhinus canicula) and characterize the expression of conserved genes known to mediate dental development. We find that shark denticle development shares a vast gene expression signature with developing teeth. However, denticles have restricted regenerative potential, as they lack a sox2+ stem cell niche associated with the maintenance of a dental lamina, an essential requirement for continuous tooth replacement. We compare developing denticles to other skin appendages, including both sensory skin appendages and avian feathers. This reveals that denticles are not only tooth-like in structure, but that they also share an ancient developmental gene set that is likely common to all epidermal appendages.