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121.
Schmelting B Zimmermann E Berke O Bruford MW Radespiel U 《American journal of physical anthropology》2007,133(1):743-752
Male mating tactics can vary according to the potential for scramble or contest competition but also as a consequence of individual characteristics, such as body condition and previous experience. The influence of experience, i.e., residency, on male recapture rates and reproductive success was studied in a population of free-living grey mouse lemurs. Long-term capture data from 320 individuals revealed that both sexes had very low recapture probabilities within their first year in the study population, but recapture rates declined less sharply during the following years. Capture results and telemetric analyses on 12 focal males revealed that resident males had larger body mass and larger home ranges than new males. Home range size correlated with the number of accessible females, indicating that resident males had higher probabilities to meet mates than new males. The reproductive success of 132 candidate fathers, representing both resident and new males, was determined by means of molecular genotyping. Paternity determination was successful in 38 cases (success rate: 19%). Sixteen resident males and seventeen new males sired offspring. However, in relation to the number of candidate fathers being present in the mating season, resident males were twice as likely to reproduce successfully as new males. These findings suggest experience-dependent reproductive tactics that most likely correspond to a differential spatial knowledge of resources, mates and potential threats. The results generally agree with the predictions made for a scramble competition regime and demonstrate substantial behavioral plasticity in a nocturnal primate species with a dispersed multi-male/multi-female mating system. 相似文献
122.
Burgermeister E Chuderland D Hanoch T Meyer M Liscovitch M Seger R 《Molecular and cellular biology》2007,27(3):803-817
The mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) cascade plays a central role in intracellular signaling by many extracellular stimuli. One target of the ERK cascade is peroxisome proliferator-activated receptor gamma (PPARgamma), a nuclear receptor that promotes differentiation and apoptosis. It was previously demonstrated that PPARgamma activity is attenuated upon mitogenic stimulation due to phosphorylation of its Ser84 by ERKs. Here we show that stimulation by tetradecanoyl phorbol acetate (TPA) attenuates PPARgamma's activity in a MEK-dependent manner, even when Ser84 is mutated to Ala. To elucidate the mechanism of attenuation, we found that PPARgamma directly interacts with MEKs, which are the activators of ERKs, but not with ERKs themselves, both in vivo and in vitro. This interaction is facilitated by MEKs' phosphorylation and is mediated by the basic D domain of MEK1 and the AF2 domain of PPARgamma. Immunofluorescence microscopy and subcellular fractionation revealed that MEK1 exports PPARgamma from the nucleus, and this finding was supported by small interfering RNA knockdown of MEK1 and use of a cell-permeable interaction-blocking peptide, which prevented TPA-induced export of PPARgamma from the nucleus. Thus, we show here a novel mode of downregulation of PPARgamma by its MEK-dependent redistribution from the nucleus to the cytosol. This unanticipated role for the stimulation-induced nuclear shuttling of MEKs shows that MEKs can regulate additional signaling components besides the ERK cascade. 相似文献
123.
SOS induction in a subpopulation of structural maintenance of chromosome (Smc) mutant cells in Bacillus subtilis
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The structural maintenance of chromosome (Smc) protein is highly conserved and involved in chromosome compaction, cohesion, and other DNA-related processes. In Bacillus subtilis, smc null mutations cause defects in DNA supercoiling, chromosome compaction, and chromosome partitioning. We investigated the effects of smc mutations on global gene expression in B. subtilis using DNA microarrays. We found that an smc null mutation caused partial induction of the SOS response, including induction of the defective prophage PBSX. Analysis of SOS and phage gene expression in single cells indicated that approximately 1% of smc mutants have fully induced SOS and PBSX gene expression while the other 99% of cells appear to have little or no expression. We found that induction of PBSX was not responsible for the chromosome partitioning or compaction defects of smc mutants. Similar inductions of the SOS response and PBSX were observed in cells depleted of topoisomerase I, an enzyme that relaxes negatively supercoiled DNA. 相似文献
124.
Simone Schehka Karl-Heinz Esser Elke Zimmermann 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》2007,193(8):845-852
Empirical research on human and non-human primates suggests that communication sounds express the intensity of an emotional
state of a signaller. In the present study, we have examined communication sounds during induced social interactions of a
monogamous mammal, the tree shrew. To signal their unwillingness to mate, female tree shrews show defensive threat displays
towards unfamiliar males paralleled by acoustically variable squeaks. We assumed that the distance between interacting partners
as well as the behavior of the male towards the female indicates the intensity of perceived social threat and thereby the
arousal state of a female. To explore this hypothesis we analyzed dynamic changes in communication sounds uttered during induced
social interactions between a female and an unfamiliar male. Detailed videographic and sound analyzes revealed that the arousal
state predicted variations in communication sound structure reliably. Both, a decrease of distance and a male approaching
the female led to an increase in fundamental frequency and repetition rate of syllables. These findings support comparable
results in human and non-human primates and suggest that common coding rules in communication sounds govern acoustic conflict
regulation in mammals. 相似文献
125.
Synthetic cryptolepine inhibits DNA binding of NF-kappaB 总被引:1,自引:0,他引:1
126.
127.
128.
Noens EE Mersinias V Willemse J Traag BA Laing E Chater KF Smith CP Koerten HK van Wezel GP 《Molecular microbiology》2007,64(5):1244-1259
129.
Mathias Craul Elke Zimmermann Solofonirina Rasoloharijaona Blanchard Randrianambinina Ute Radespiel 《BMC evolutionary biology》2007,7(1):83
Background
The lemurs of Madagascar provide an excellent mammalian radiation to explore mechanisms and processes favouring species diversity and evolution. Species diversity, in particular of nocturnal species, increased considerably during the last decade. However, the factors contributing to this high diversity are not well understood. We tested predictions derived from two existing biogeographic models by exploring the genetic and morphological divergence among populations of a widely distributed lemur genus, the sportive lemur (Lepilemur ssp.) along a 560 km long transect from western to northern Madagascar. 相似文献130.
Inducible gene expression with the Tet-on system in CD4+ T cells and thymocytes of mice 总被引:1,自引:0,他引:1
CD4+ T cells with their growing list of effector and regulatory subpopulations have vital functions within the immunohematopoietic system. We report here on the first mouse lines that allow temporally and quantitatively controlled expression of transgenes specifically in CD4+ thymocytes and T cells. These were constructed using the Tet-on system. The rtTA2(S)-M2 version of the reverse tetracycline-dependent transactivator was placed under control of all known CD4 regulatory elements. Reporter transgene expression in mice expressing these constructs is highly specific for CD4+ cells, is strictly dependent on the tetracycline derivative doxycycline, and can be regulated by up to five logs depending on the doxycycline concentration. Moreover, we demonstrate that these mice can be used for noninvasive in vivo imaging of a coexpressed luciferase reporter. These new mouse lines should be highly valuable for studying and manipulating numerous aspects of CD4+ T cell development, biology, and function. 相似文献