全文获取类型
收费全文 | 398篇 |
免费 | 29篇 |
出版年
2024年 | 1篇 |
2023年 | 1篇 |
2022年 | 3篇 |
2021年 | 13篇 |
2020年 | 9篇 |
2019年 | 7篇 |
2018年 | 7篇 |
2017年 | 7篇 |
2016年 | 12篇 |
2015年 | 28篇 |
2014年 | 29篇 |
2013年 | 38篇 |
2012年 | 39篇 |
2011年 | 53篇 |
2010年 | 20篇 |
2009年 | 15篇 |
2008年 | 28篇 |
2007年 | 26篇 |
2006年 | 20篇 |
2005年 | 6篇 |
2004年 | 16篇 |
2003年 | 8篇 |
2002年 | 10篇 |
2000年 | 3篇 |
1999年 | 3篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1991年 | 2篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 2篇 |
1983年 | 1篇 |
1978年 | 1篇 |
1976年 | 1篇 |
1974年 | 2篇 |
1973年 | 4篇 |
1970年 | 1篇 |
排序方式: 共有427条查询结果,搜索用时 31 毫秒
81.
Maki EC Millar JG Rodstein J Hanks LM Barbour JD 《Journal of economic entomology》2011,104(3):933-938
Larvae of Prionus californicus Motschulsky (Coleoptera: Cerambycidae) feed on the roots of many types of woody perennial crops and are serious pests of hop in the northwestern United States. The adult males are strongly attracted to a volatile sex pheromone, (3R,5S)-3,5-dimethyldodecanoic acid, that is produced by females. Here, we summarize the results of field experiments that evaluated the potential for using the synthetic pheromone (in a blend of all four possible stereoisomers) to manage infestations of P. californicus in commercial hop yards by mass trapping or mating disruption. Our research provides evidence that mass trapping may be effective in reducing mating success of the females: positioning surrogate females (sentinel traps baited with a low dose of pheromone) within a square of eight pheromone-baited traps resulted in an 88% reduction in the number of wild males that reached the sentinel traps compared with sentinel traps that were surrounded by traps baited with blank lures. Similarly, surrogate females that were surrounded by pheromone lures (without traps) were reached by 84% fewer wild males than surrogate females surrounded by blank lures, suggesting that mating disruption also may be effective. A mark-recapture experiment indicated that male P. californicus were attracted to traps baited with 1 mg of pheromone from as far away as 585 m. These studies indicate that 3,5-dimethyldodecanoic acid has very good potential for managing P. californicus in hop yards, and perhaps in other crops where it is a pest. 相似文献
82.
Bonsignori M Hwang KK Chen X Tsao CY Morris L Gray E Marshall DJ Crump JA Kapiga SH Sam NE Sinangil F Pancera M Yongping Y Zhang B Zhu J Kwong PD O'Dell S Mascola JR Wu L Nabel GJ Phogat S Seaman MS Whitesides JF Moody MA Kelsoe G Yang X Sodroski J Shaw GM Montefiori DC Kepler TB Tomaras GD Alam SM Liao HX Haynes BF 《Journal of virology》2011,85(19):9998-10009
V2/V3 conformational epitope antibodies that broadly neutralize HIV-1 (PG9 and PG16) have been recently described. Since an elicitation of previously known broadly neutralizing antibodies has proven elusive, the induction of antibodies with such specificity is an important goal for HIV-1 vaccine development. A critical question is which immunogens and vaccine formulations might be used to trigger and drive the development of memory B cell precursors with V2/V3 conformational epitope specificity. In this paper we identified a clonal lineage of four V2/V3 conformational epitope broadly neutralizing antibodies (CH01 to CH04) from an African HIV-1-infected broad neutralizer and inferred their common reverted unmutated ancestor (RUA) antibodies. While conformational epitope antibodies rarely bind recombinant Env monomers, a screen of 32 recombinant envelopes for binding to the CH01 to CH04 antibodies showed monoclonal antibody (MAb) binding to the E.A244 gp120 Env and to chronic Env AE.CM243; MAbs CH01 and CH02 also bound to transmitted/founder Env B.9021. CH01 to CH04 neutralized 38% to 49% of a panel of 91 HIV-1 tier 2 pseudoviruses, while the RUAs neutralized only 16% of HIV-1 isolates. Although the reverted unmutated ancestors showed restricted neutralizing activity, they retained the ability to bind to the E.A244 gp120 HIV-1 envelope with an affinity predicted to trigger B cell development. Thus, E.A244, B.9021, and AE.CM243 Envs are three potential immunogen candidates for studies aimed at defining strategies to induce V2/V3 conformational epitope-specific antibodies. 相似文献
83.
84.
Bladder cancer is diagnosed by cystoscopy, a costly and invasive procedure that is associated with patient discomfort. Analysis of tumor-specific markers in DNA from sediments of voided urine has the potential for non-invasive detection of bladder cancer; however, the sensitivity is limited by low fractions and small numbers of tumor cells exfoliated into the urine from low-grade tumors. The purpose of this study was to improve the sensitivity for non-invasive detection of bladder cancer by size-based capture and enrichment of tumor cells in urine. In a split-sample set-up, urine from a consecutive series of patients with primary or recurrent bladder tumors (N = 189) was processed by microfiltration using a membrane filter with a defined pore-size, and sedimentation by centrifugation, respectively. DNA from the samples was analyzed for seven bladder tumor-associated methylation markers using MethyLight and pyrosequencing assays. The fraction of tumor-derived DNA was higher in the filter samples than in the corresponding sediments for all markers (p<0.000001). Across all tumor stages, the number of cases positive for one or more markers was 87% in filter samples compared to 80% in the corresponding sediments. The largest increase in sensitivity was achieved in low-grade Ta tumors, with 82 out of 98 cases positive in the filter samples (84%) versus 74 out of 98 in the sediments (75%). Our results show that pre-analytic processing of voided urine by size-based filtration can increase the sensitivity for DNA-based detection of bladder cancer. 相似文献
85.
86.
87.
Elin T?rnqvist Anita Annas Britta Granath Elisabeth Jalkesten Ian Cotgreave Mattias ?berg 《PloS one》2014,9(7)
The principles of the 3Rs, Replacement, Reduction and Refinement, are being increasingly incorporated into legislations, guidelines and practice of animal experiments in order to safeguard animal welfare. In the present study we have studied the systematic application of 3R principles to toxicological research in the pharmaceutical industry, with particular focus on achieving reductions in animal numbers used in regulatory and investigatory in vivo studies. The work also details major factors influencing these reductions including the conception of ideas, cross-departmental working and acceptance into the work process. Data from 36 reduction projects were collected retrospectively from work between 2006 and 2010. Substantial reduction in animal use was achieved by different strategies, including improved study design, method development and project coordination. Major animal savings were shown in both regulatory and investigative safety studies. If a similar (i.e. 53%) reduction had been achieved simultaneously within the twelve largest pharmaceutical companies, the equivalent reduction world-wide would be about 150,000 rats annually. The results point at the importance of a strong 3R culture, with scientific engagement, collaboration and a responsive management being vital components. A strong commitment in leadership for the 3R is recommended to be translated into cross-department and inter-profession involvement in projects for innovation, validation and implementation. Synergies between all the three Rs are observed and conclude that in silico-, in vitro- and in vivo-methods all hold the potential for applying the reduction R and should be consequently coordinated at a strategic level. 相似文献
88.
Hannah H. E. Van Zanten Mario Herrero Ollie Van Hal Elin Röös Adrian Muller Tara Garnett Pierre J. Gerber Christian Schader Imke J. M. De Boer 《Global Change Biology》2018,24(9):4185-4194
The need for more sustainable production and consumption of animal source food (ASF) is central to the achievement of the sustainable development goals: within this context, wise use of land is a core challenge and concern. A key question in feeding the future world is: how much ASF should we eat? We demonstrate that livestock raised under the circular economy concept could provide a significant, nonnegligible part (9–23 g/per capita) of our daily protein needs (~50–60 g/per capita). This livestock then would not consume human‐edible biomass, such as grains, but mainly convert leftovers from arable land and grass resources into valuable food, implying that production of livestock feed is largely decoupled from arable land. The availability of these biomass streams for livestock then determines the boundaries for livestock production and consumption. Under this concept, the competition for land for feed or food would be minimized and compared to no ASF, including some ASF in the human diet could free up about one quarter of global arable land. Our results also demonstrate that restricted growth in consumption of ASF in Africa and Asia would be feasible under these boundary conditions, while reductions in the rest of the world would be necessary to meet land use sustainability criteria. Managing this expansion and contraction of future consumption of ASF is essential for achieving sustainable nutrition security. 相似文献
89.
Elin Videvall Maria Strandh Anel Engelbrecht Schalk Cloete Charlie K. Cornwallis 《Molecular ecology resources》2018,18(3):424-434
The gut microbiomes of birds and other animals are increasingly being studied in ecological and evolutionary contexts. Numerous studies on birds and reptiles have made inferences about gut microbiota using cloacal sampling; however, it is not known whether the bacterial community of the cloaca provides an accurate representation of the gut microbiome. We examined the accuracy with which cloacal swabs and faecal samples measure the microbiota in three different parts of the gastrointestinal tract (ileum, caecum, and colon) using a case study on juvenile ostriches, Struthio camelus, and high‐throughput 16S rRNA sequencing. We found that faeces were significantly better than cloacal swabs in representing the bacterial community of the colon. Cloacal samples had a higher abundance of Gammaproteobacteria and fewer Clostridia relative to the gut and faecal samples. However, both faecal and cloacal samples were poor representatives of the microbial communities in the caecum and ileum. Furthermore, the accuracy of each sampling method in measuring the abundance of different bacterial taxa was highly variable: Bacteroidetes was the most highly correlated phylum between all three gut sections and both methods, whereas Actinobacteria, for example, was only strongly correlated between faecal and colon samples. Based on our results, we recommend sampling faeces, whenever possible, as this sample type provides the most accurate assessment of the colon microbiome. The fact that neither sampling technique accurately portrayed the bacterial community of the ileum nor the caecum illustrates the difficulty in noninvasively monitoring gut bacteria located further up in the gastrointestinal tract. These results have important implications for the interpretation of avian gut microbiome studies. 相似文献
90.
Surface display of a functional single-chain Fv antibody on staphylococci. 总被引:13,自引:2,他引:11 下载免费PDF全文
Two different host-vector expression systems designed for cell surface display of chimeric receptors on Staphylococcus xylosus and Staphylococcus carnosus have been evaluated for surface display of a mouse immunoglobulin G1(kappa) [IgG1(kappa)] anti-human IgE single-chain Fv (scFv) antibody fragment. To achieve surface anchoring of the chimeric receptors containing the scFv, the cell surface attachment regions from Staphylococcus aureus protein A were used in both expression systems. The different chimeric receptors could be recovered from cell wall extracts of both S. xylosus and S. carnosus, and surface localization was demonstrated by taking advantage of a serum albumin-binding reporter region present within the two types of receptors. In addition, the two different recombinant staphylococci carrying hybrid receptors containing the scFv were demonstrated to react with the antigen, which was human IgE, in whole-cell enzyme-linked immunosorbent assays. This is the first report of an antibody fragment expressed in a functional form anchored to the surface of gram-positive bacteria. The potential use of recombinant gram-positive bacteria as whole-cell diagnostic devices or alternatives to filamentous phages for surface display of scFv libraries is discussed. 相似文献