全文获取类型
收费全文 | 1356篇 |
免费 | 139篇 |
国内免费 | 1篇 |
专业分类
1496篇 |
出版年
2023年 | 8篇 |
2022年 | 12篇 |
2021年 | 28篇 |
2020年 | 22篇 |
2019年 | 29篇 |
2018年 | 34篇 |
2017年 | 30篇 |
2016年 | 50篇 |
2015年 | 81篇 |
2014年 | 75篇 |
2013年 | 64篇 |
2012年 | 82篇 |
2011年 | 95篇 |
2010年 | 46篇 |
2009年 | 43篇 |
2008年 | 73篇 |
2007年 | 53篇 |
2006年 | 67篇 |
2005年 | 58篇 |
2004年 | 52篇 |
2003年 | 45篇 |
2002年 | 39篇 |
2001年 | 11篇 |
2000年 | 18篇 |
1999年 | 23篇 |
1998年 | 13篇 |
1997年 | 14篇 |
1995年 | 8篇 |
1994年 | 11篇 |
1993年 | 8篇 |
1992年 | 15篇 |
1991年 | 20篇 |
1990年 | 11篇 |
1989年 | 18篇 |
1988年 | 10篇 |
1987年 | 13篇 |
1986年 | 9篇 |
1985年 | 11篇 |
1984年 | 9篇 |
1983年 | 8篇 |
1982年 | 16篇 |
1980年 | 9篇 |
1979年 | 10篇 |
1978年 | 8篇 |
1975年 | 8篇 |
1974年 | 9篇 |
1973年 | 11篇 |
1972年 | 11篇 |
1968年 | 7篇 |
1962年 | 8篇 |
排序方式: 共有1496条查询结果,搜索用时 15 毫秒
11.
Curves relating net photosynthetic rate to irradiance [P(I) curve relation] were estimated and analysed inMercurialis perennis L. plants stemming from three forest (spruce, beech and ash) stands with different tree leaf canopy development and different
light regime.
The saturating irradiance (Is) reached the highest values in plants of all three stands in spring (spruce forest: 438 W m−2, beech forest: 440 W m−2 and ash forest: 367 W m−2), it declined sharply in the middle of the growing season (283, 285 and 297 W m-2, respectively) and this Is level persisted until autumn. A pronounced dynamics in plants from spruce and beech forests made itself manifest also in
the adaptation (Ia) and compensating (Ic) irradiances, respectively. After a sudden decline in summer, values in autumn were close to those of the vernal season.
The most pronounced parameter, which optimally expressed the adaptation ofMercurialis perennis to various light conditions, was the photosynthetic efficiency (α) calculated as the slope of the linear part of the curve
relating net photosynthetic rate to irradiance.
At the time of the highest PN sat. value in course of the growing season (August) (spruce forest: 100, beech forest: 98.7 and ash forest: 85.8 μg CO2 m−2 s−1), RD was in its minimum; in autumn PN sat. reached the lowest values which corresponded to the most intensive RD.
It was found thatMercurialis perennis plants stemming from forest stands with different light conditions did not make use equally of the altering light conditions
in the course of the growing season. By the underlying analysis of P(I) curves this rhizomatous perennial herb (geophyte)
may be characterized as a shade tolerant species. 相似文献
12.
Abstract: The possibility that γ-hydroxybutyrate (GHB), a metabolite of γ-aminobutyric acid (GABA), may play a role in the CNS has recently come to attention. We describe here a sensitive and specific mass fragmento-graphic technique that allows the measurement of picomole amounts of GHB in single rat brain areas. Moreover, we show that GHB can accumulate postmortem, an effect that is blocked by the use of microwave irradiation to kill the animals. To understand further the relationship between GABA and GHB formation, we treated rats with drugs known to inferfere with GABA metabolism at different levels and concomitantly measured GABA and GHB in cerebral cortex and cerebellum. Isoniazide, which blocks the formation of GABA, also decreases GHB. Blockers of the catabolism of GABA, such as aminooxyacetic acid and γ-acetylenic GABA, increase GABA levels and decrease those of GHB. Sodium dipropylacetate increases both GABA and GHB, supporting the hypothesis that this effective antiepileptic drug also blocks in vivo the enzyme that converts succinic semialdehyde to succinic acid. 相似文献
13.
14.
The population of a biological system in a sewage treatment plant is formed of a fortuitous assemblage of microorganisms, members of which are continually dropped out while additional members are recruited. Many of these organisms are active throughout the year. Fewer yeasts than filamentous fungi are found by the plating technique. The population is about evenly divided between species producing their spores in dry states and in wet states. The fungus population includes species which under certain circumstances are able to use a wide variety of substrates from simple sugars to complex carbohydrates and hydrocarbons.
Much of the material included here was Presented at the 23rd Industrial Waste Conference Purdue University, Lafayette, Indiana May 7–9, 1968 相似文献
Zusammenfassung Die Population eines biologischen Systems in einem Kloakenwasser-Behandlungs-werk ist durch ein zufälliges Zusammentreffen von Mikroorganismen geformt, deren Mitglieder fortwährend ausfallen, während andere hinzukommen. Viele dieser Mikroorganismen sind während des ganzen Jahres aktiv. Weniger Hefen denn Fadenpilze sind mit der Plattentechnique gefunden. Die Population ist ungefähr gleichmäßig unter den Arten verteilt, die die Sporen in trockenem wie in feuchtem Zustand produzieren. Die Pilzpopulation umfaßt Arten, die unter gewissen Umständen fähig sind, eine große Varietät von Nährmaterial, von einfachen wie auch komplexen Kohlenstoffhydraten und Kohlenstoffhydraten zu benutzen.
Much of the material included here was Presented at the 23rd Industrial Waste Conference Purdue University, Lafayette, Indiana May 7–9, 1968 相似文献
15.
An azaserine-resistant derivative of Escherichia coli B/UV, AZA/R(1), was found to carry a mutator gene. This gene, designated mutS1, was mapped by means of conjugation and P1kc-mediated transduction. The mutS1 gene was cotransduced with argB at a frequency of 2.4%; the gene order in this region of the chromosome is thy argB mutS1. To determine whether a relationship commonly exists between azaserine resistance and the mutator property, 12 additional azaserine-resistant derivatives of B/UV were developed and tested for the mutator phenotype. None of the twelve was a mutator strain. The level of azaserine resistance was not increased over that of the recipient parent when mutS1 was transduced to an azaserine-susceptible strain. Reversion studies indicated that mutS1 induced adenosine-ribosylthymine to guanosine-cytidine and guanosine-cytidine to adenosine-ribosylthymine transitions. Because such mutational changes are suppressible with deoxynucleosides when induced by base analogues, an attempt was made to suppress the mutator activity of mutS1 by the addition of deoxyribonucleosides to the medium. No suppression was found. Recombinants were prepared containing mutS1 and the Treffers mutator gene of E. coli K-12. The effect of the mutator genes appears to be additive. 相似文献
16.
17.
Eli Shapiro Steven D. Brown Alan R. Saltiel James H. Schwartz 《Developmental neurobiology》1991,22(1):55-62
In mollusks as in other animals, peptides can act as hormones, growth factors, and neurotransmitters. The presence of insulin in vertebrate brain as well as its actions on nerve cells led us to examine the electrophysiological effects of the mammalian hormone on Aplysia neurons. Application of insulin extracellularly causes hyperpolarization of L14 and L10, identified neurons of the abdominal ganglion. This hyperpolarization is associated with a decreased membrane conductance that reverses at ?35 mV. We also injected inositol phosphate glycan (IPG) into the identified neurons. This complex sugar, which was purified from rat liver and which is a putative second messenger for insulin in nonneural vertebrate cells (Saltiel and Cuatrecasas, 1986; Saltiel, Osterman, and Darnell, 1988), causes hyperpolarization with decreased membrane conductance in L14 and L10 similar to the effects of insulin. Furthermore, exposure of isolated ganglia to insulin results in the generation of IPG with a compensating decrease in its glycosyl-phosphatidylinositol precursor. We suggest that, in addition to its other roles, insulin may function as a neuropeptide transmitter using IPG as a second messenger. 相似文献
18.
Stand microclimate and physiological activity of tree leaves in an oak-hornbeam forest 总被引:1,自引:0,他引:1
Michal Marek Elena Masarovičovà Irena Kratochvílová P. Eliáš D. Janouš 《Trees - Structure and Function》1989,3(4):234-240
Summary In an uneven-aged, multi-species oak-hornbeam forest at Báb, SW Slovakia (former IBP Forest Research Site), a series of micrometeorological and ecophysiological measurements started in 1985. The aims of the work are to improve understanding of physiological processes (photosynthesis, respiration, and transpiration) of adult trees and stand microclimate, to collect data for simulation of the canopy (stand) photosynthesis and for ecological synthesis of the functioning of the forest ecosystem. In this paper, photosynthetically active radiation (PAR), air temperature (AT) and relative humidity (RH), wind speed (WS), and CO2 concentration ([CO2]) in and above the forest are characterized for the fully leaved season, using diurnal courses, vertical profiles and isodiagrams (isopleths). Approximately 50% of incident PAR was absorbed by the upper 4–5 m layer of leaves and only approximately 5% or less penetrated to the forest floor. Vertical gradients of AT and RH were generally low, but large differences in diurnal ranges of AT and RH were observed between vertical levels. The upper leaf canopy greatly reduced WS, and at a height of about 14 m above the ground it was close to zero. The highest diurnal [CO2] maximum and variations occurred at 1 m above the ground, and the lowest above the forest. In good light conditions in the forest, the entire leaf canopy (overstorey and understorey canopy) is a large sink of CO2. At night the forest stand is a source of CO2, the largest internal source being the soil and forest floor. 相似文献
19.
Graźyna Zimowska Paul David Shirk Donald LeRoy Silhacek Eli Shaaya 《Development genes and evolution》1994,203(4):215-226
We describe a provitellogenic stage, a previously unrecognized stage of follicle development in moths, and show that oocytes begin yolk sphere formation prior to the development of patency by the follicular epithelium. The vitellogenic activities of follicles from pharate adult femalePlodia interpunctella (Hübner) were determined by visualizing the subunits of vitellin (YP1 and YP3) and the follicular epithelium yolk protein (YP2 and YP4) using monospecific antisera to each subunit to immunolabel whole-mounted ovaries or ultrathin sections. At 92 h after pupation, yolk spheres that contained only YP2 began to proliferate in the oocytes. The inter-follicular epithelial cell spaces were closed at 92 h making vitellogenin inaccessible to the oocyte, and consequently, the vitellin subunits were not observed in the yolk spheres. YP2 uptake most likely occurred across the brush border from the follicular epithelial cells to the oocyte at this time. At 105 h, the inter-follicular epithelial cell spaces appeared closed yet trace amounts of labeling for vitellin were observed in the spaces and also in the yolk spheres along with YP2. Equivalent labeling for all four YPs in yolk spheres was finally observed at 112 h after pupation when the follicular epithelium had become patent. These data indicate that the provitellogenic stage is an extended transition period between the previtellogenic and vitellogenic stages that lasts for approximately 13 h, and it is marked at the beginning by YP2 yolk sphere formation in the oocyte and at the end by patency in the follicular epithelium. 相似文献
20.
Subjects made fast goal-directed arm movements towards moving targets. In some cases, the perceived direction of target motion
was manipulated by moving the background. By comparing the trajectories towards moving targets with those towards static targets,
we determined the position towards which subjects were aiming at movement onset. We showed that this position was an extrapolation
in the target’s perceived direction from its position at that moment using its perceived direction of motion. If subjects
were to continue to extrapolate in the perceived direction of target motion from the position at which they perceive the target
at each instant, the error would decrease during the movements. By analysing the differences between subjects’ arm movements
towards targets moving in different (apparent) directions with a linear second-order model, we show that the reduction in
the error that this predicts is not enough to explain how subjects compensate for their initial misjudgements.
Received: 10 February 1995/Accepted in revised form: 30 May 1995 相似文献