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191.
Elham Ghadami Novin Nikbakhsh Sadegh Fattahi Mohadeseh Kosari-Monfared Mohammad Ranaee Hassan Taheri Fatemeh Amjadi-Moheb Gholamali Godazandeh Shahryar Shafaei Anahita Nosrati Maryam Pilehchian Langroudi Ali Akbar Samadani Galia Amirbozorgi Vahideh Mirnia Haleh Akhavan-Niaki 《Journal of cellular physiology》2019,234(4):4115-4124
Gastric cancer (GC) is one of the most common causes of cancer-related death in the world, with multiple genetic and epigenetic alterations involved in disease development. CYLD tumor suppressor gene encodes a multifunctional deubiquitinase which negatively regulates various signaling pathways. Deregulation of this gene has been found in different types of cancer. This study aimed to evaluate for the first time the CpG island methylation pattern of CYLD gene promoter, and its expression level in gastric adenocarcinoma. CYLD messenger RNA expression and promoter methylation in 53 tumoral and their non-neoplastic counterpart tissues were assessed using quantitative polymerase chain reaction and bisulfite sequencing. Also, we investigated the impacts of the infectious agents including Helicobacter pylori (H. pylori), EBV, and CMV on CYLD expression and promoter methylation in GC. Results showed that the expression level of CYLD was downregulated in GC, and was significantly associated with gender (female), patient’s age (<60), high grade, and no lymph-node metastasis (p = 0.001, 0.002, 0.03, and 0.003, respectively). Among the 31 analyzed CpG sites located in about 600 bp region within the promoter, two CpG sites were hypermethylated in GC tissues. We also found a significant inverse association between DNA promoter methylation and CYLD expression (p = 0.02). Furthermore, a direct association between H. pylori, EBV, and CMV infections with hypermethylation and reduced CYLD expression was observed (p = 0.04, 0.03, and 0.03, respectively). Our findings indicate that CYLD is downregulated in GC. Infectious agents may influence CYLD expression. 相似文献
192.
Samira Basharkhah Faezeh Sabet Sanaz Ahmadi Ghezeldasht Arman Mosavat Hamid Reza Jahantigh Elham Barati Khosrow Shamsian Masoud Saleh‐Moghaddam Hiva Sharebyani Tahereh Hassannia Seyed Ali Akbar Shamsian 《Microbiology and immunology》2019,63(11):449-457
Hepatitis C virus (HCV) infection is a major public health problem with about 1.75 million new HCV cases and 71 million chronic HCV infections worldwide. The study aimed to evaluate clinical, serological, molecular, and liver markers to develop a mathematical predictive model for the quantification of the HCV viral load in chronic HCV infected patients. In this cross‐sectional study, blood samples were taken from 249 recently diagnosed HCV‐infected subjects and were tested for liver condition, viral genotype, and HCV RNA load. Receiver operating characteristics (ROC) curves and multiple linear regression analysis were used to predict the HCV‐RNA load. Genotype 3 followed by genotype 1 were the most prevalent genotypes in Mashhad, Northeastern Iran. The maximum levels of viral load were detected in the mixed genotype group, and the lowest levels in the undetectable genotype group. The log of the HCV viral load was significantly associated with thrombocytopenia and higher serum levels of alanine transaminase (ALT). In addition, the log HCV RNA was significantly higher in patients with arthralgia, fatigue, fever, vomiting, or dizziness. Moreover, genotype 3 was significantly associated with icterus. A ROC curve analysis revealed that the best cut‐off points for serum levels of aspartate aminotransferase (AST), ALT, and alkaline phosphatase (ALP) were >31, >34, and ≤246 IU/L, respectively. Sensitivity, specificity, and positive predictive values for AST were 87.7%, 84.36%, and 44.6%, for ALT they were 83.51%, 81.11%, and 36%, and for ALP were 72.06%, 42.81%, and 8.3%, respectively. A mathematical regression model was developed that could estimate the HCV‐RNA load. Regression model: log viral load = 7.69 ? 1.01 × G3 ? 0.7 × G1 + 0.002 × ALT ? 0.86 × fatigue. 相似文献
193.
Ramazani Elham Fereidoni Masoud Tayarani-Najaran Zahra 《Molecular biology reports》2019,46(6):5777-5783
Molecular Biology Reports - Neuroprotection using compounds with dual functions of anti-apoptotic and antioxidant effects fight against neurodegeneration. Vitamin K2 acts as a cofactor in many... 相似文献
194.
Elham Afzali Touba Eslaminejad Sahar Amirpour-Rostami 《Biocatalysis and Biotransformation》2019,37(3):201-209
The main aim of this study was to synthesize the superparamagnetic nanoparticles coated by alginate/chitosan/β-cyclodextrin to purify α-amylase. Isolated bacteria were identified by morphological, biochemical and taxonomic molecular studies. FTIR- spectrometer, VSM, X-ray instruments and Malvern Zetasizer were used to characterize nanoparticles characteristics. The morphological structures and the elemental composition of the nanoparticles were studied by using FESEM and EDS, respectively. The molecular weight of enzyme was determined using SDS-PAGE, and the enzyme activity detected by zymographic analysis. FTIR studies showed the presence of Fe–O–Fe in the Fe3O4 and verified the interaction between chitosan, β-cyclodextrin and alginate. The saturation magnetization for superparamagnetic and coated superparamagnetic nanoparticles was indicated 39 and 1.9?emu?g?1, respectively. The maximum intensity of the XRD peak indicated the presence of the Fe3O4. FESEM and EDS analysis showed that the nanoparticles were regular and spherical in shape and corresponded to the Fe and O elements. Enzyme purification by synthesized nanoparticles was achieved 13.84?U?mg?1; purification fold of 3.50. The molecular weight of α-amylase was about 22?kDa. The highest activity of α-amylase was observed at 70?°C, pH 9.3 and Ca2+-independent. As a conclusion, the coated superparamagnetic nanoparticles showed more applications in enzyme purification comparing to the conventional methods. 相似文献
195.
196.
Saeid Shahrabi Zeinab D. Zayeri Narges Ansari Elham H. Hadad Elham Rajaei 《Journal of cellular physiology》2019,234(10):16998-17010
Natural killer (NK) cells play an essential role in the immune response to infections, inflammations, and malignancies. Recent studies suggest that NK cell surface receptors and cytokines are the key points of the disease development and protection. We hypothesized that the interactions between NK cell receptors and targeted cells construct an eventual niche, and this niche has an eventual profile in various autoimmune diseases and cancers. The NK cells preactivated with cytokines, such as interleukin-2 (IL-2), IL-12, IL-15, and IL-18 can have higher cytotoxicity; however, the toxic side effect of IL-2 should be considered. The vicissitudes of NK cell profile and its receptors obey the environmental communications and cell interactions. Our vision around the NK cells as an immune axis remained dual, and we still cannot judge the immune responses based on the NK cell flip-flop. A design of eventual niche to monitor the NK cell and targeted cell interaction is needed to strengthen our ability in diagnosis and treatment approaches based on the NK cells. Here, we have reviewed the shifts in the NK cells and their surface receptors in autoimmune diseases, solid tumors, and leukemia, and also discussed the effective chemokines that affect NK cell activation and proliferation. The main aim of this review is to present a broader vision of the NK cell changes in autoimmune disease and cancers. 相似文献
197.
Tanja Miloti Christophe Baltzinger Carsten Eichberg Amy E. Eycott Marco Heurich Jrg Müller Jorge A. Noriega Rosa Menendez Jutta Stadler Rka dm Tessa Bargmann Isabelle Bilger Jrn Buse Joaquín Calatayud Constantin Ciubuc Gergely Boros Pierre Jay‐Robert Mrt Kruus Enno Merivee Geoffrey Miessen Anne Must Elham Ardali Elena Preda Iraj Rahimi Dirk Rohwedder Rob Rose Eleanor M. Slade Lszl Somay Pejman Tahmasebi Stefano Ziani Maurice Hoffmann 《Journal of Biogeography》2019,46(1):70-82
198.
Abbas Nasehi Mehdi Nasr Esfahani Farshid Mahmodi Elham Golkhandan Shamima Akter 《Archives Of Phytopathology And Plant Protection》2013,46(14):1658-1665
During 2011–2012, an extensive leaf spot disease caused by Stemphylium lycopersici was observed on vegetable crops including, tomato, eggplant, pepper and lettuce in major vegetable-growing regions of Malaysia. Four isolates of S. lycopersici obtained from each vegetable crop were used to determine cultural and physiological characteristics. The variations were found in colony colour (pale to light grey or light as well as the brown), texture (cottony or mycelium flat), shape (regular with concentric growth rings or irregular) and pigmentation (yellow or deep red) of the cultures. The optimum temperature for the conidial germination and mean radial growth of the isolates was 25?°C, and the radial growth of the isolates was maximal on V-8 juice agar followed by potato carrot agar. The maximum sporulation of S. lycopersici isolates was observed on V-8 juice agar media under 12/12 h light/darkness photoperiod at 25?°C. 相似文献
199.
Elham Yezdani Jalal Jalali Sendi Arash Zibaee Mohammad Ghadamyari 《Comptes rendus biologies》2010,333(1):17-22
The pyralid moth, Glyphode pyloalis Walker, is an important pest of the mulberry. Amylases are the hydrolytic enzymes that catalyze the hydrolysis of the α-D-(1,4)-glucan linkage in glycogen and other related carbohydrates. Laboratory-reared fifth stadium larvae were randomly selected; the midgut (MG) and the salivary glands (SG) were removed by dissection under a dissecting microscope and α-amylase activity was assayed using the dinitrosalicylic acid procedure. The activity of α-amylase in the MG and the SG were 0.011 and 0.0018 μmol/min, respectively. The optimal pH and temperature for α-amylase were 9 for MG at 37–40 °C and 10 for SG at 37 °C respectively. Various concentrations of compounds (NaCl, KCl, MgCl2, Urea, EDTA, SDS and CaCl2) had differential effects on the enzyme activity. Plant amylase inhibitors may play an important role against insect pests. Hence, the characterization of digestive enzymes and the examination of their inhibitors may be a useful tool in future management of this important mulberry pest. 相似文献
200.
Ali Niapour Fereshteh Karamali Khadijeh Karbalaie Abbas Kiani Mohammad Mardani Mohammad Hossein Nasr-Esfahani Hossein Baharvand 《Biotechnology letters》2010,32(6):781-786
Schwann cells (SCs) can be used to repair both the peripheral and central nervous systems. Therefore, establishment of a procedure
to obtain activated, highly proliferative SCs, in an appropriate time for clinical applications, is a prerequisite. Purification
is complicated by contamination with fibroblasts which often become the predominant cell type in an in vitro SC culture. This
study describes a novel and efficient method to enrich SCs by utilizing the differential detachment properties of the two
cell types. In culture, cells were treated with two different media and the chelator, EGTA, which detached SCs faster than
fibroblasts and allowed for easy isolation of SCs. Within seven days, high yields of SCs with a purity of greater than 99%
were achieved. This was confirmed by immunostaining characterization and flow-cytometric analyses using an antibody against
the p75 low affinity nerve growth factor receptor (p75LNGFR). The entire procedure was completed in approximately 21 days.
This method has the advantage of being technically easier, faster, and more efficient than other previously described methods.
An SC culture that was about 99% homogenous was achieved. 相似文献