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991.
Laura Mediani Francesco Antoniani Veronica Galli Jonathan Vinet Arianna Dorotea Carr Ilaria Bigi Vadreenath Tripathy Tatiana Tiago Marco Cimino Giuseppina Leo Triana Amen Daniel Kaganovich Cristina Cereda Orietta Pansarasa Jessica Mandrioli Priyanka Tripathi Dirk Troost Eleonora Aronica Johannes Buchner Anand Goswami Jared Sterneckert Simon Alberti Serena Carra 《EMBO reports》2021,22(5)
Stress granules (SGs) are dynamic condensates associated with protein misfolding diseases. They sequester stalled mRNAs and signaling factors, such as the mTORC1 subunit raptor, suggesting that SGs coordinate cell growth during and after stress. However, the molecular mechanisms linking SG dynamics and signaling remain undefined. We report that the chaperone Hsp90 is required for SG dissolution. Hsp90 binds and stabilizes the dual‐specificity tyrosine‐phosphorylation‐regulated kinase 3 (DYRK3) in the cytosol. Upon Hsp90 inhibition, DYRK3 dissociates from Hsp90 and becomes inactive. Inactive DYRK3 is subjected to two different fates: it either partitions into SGs, where it is protected from irreversible aggregation, or it is degraded. In the presence of Hsp90, DYRK3 is active and promotes SG disassembly, restoring mTORC1 signaling and translation. Thus, Hsp90 links stress adaptation and cell growth by regulating the activity of a key kinase involved in condensate disassembly and translation restoration. 相似文献
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Faltýnková Anna Kudlai Olena Salganskiy Oleksander O. Korol Eleonora M. Kuzmina Tetiana A. 《Systematic parasitology》2022,99(4):491-523
Systematic Parasitology - In 2014–2015 and 2019–2021, teleost fishes off Galindez Island (Antarctic Peninsula) were examined for trematodes. Combined morphological and molecular... 相似文献
994.
Ramona Marasco Marco Fusi Eleonora Rolli Besma Ettoumi Fulvia Tambone Sara Borin Hadda-Imene Ouzari Abdellatif Boudabous Claudia Sorlini Ameur Cherif Fabrizio Adani Daniele Daffonchio 《Environmental microbiology》2021,23(10):6275-6291
Aridity negatively affects the diversity and abundance of edaphic microbial communities and their multiple ecosystem services, ultimately impacting vegetation productivity and biotic interactions. Investigation about how plant-associated microbial communities respond to increasing aridity is of particular importance, especially in light of the global climate change predictions. To assess the effect of aridity on plant associated bacterial communities, we investigated the diversity and co-occurrence of bacteria associated with the bulk soil and the root system of olive trees cultivated in orchards located in higher, middle and lower arid regions of Tunisia. The results indicated that the selective process mediated by the plant root system is amplified with the increment of aridity, defining distinct bacterial communities, dominated by aridity-winner and aridity-loser bacteria negatively and positively correlated with increasing annual rainfall, respectively. Aridity regulated also the co-occurrence interactions among bacteria by determining specific modules enriched with one of the two categories (aridity-winners or aridity-losers), which included bacteria with multiple PGP functions against aridity. Our findings provide new insights into the process of bacterial assembly and interactions with the host plant in response to aridity, contributing to understand how the increasing aridity predicted by climate changes may affect the resilience of the plant holobiont. 相似文献
995.
Several pathogens co‐opt host intracellular compartments to survive and replicate, and they thereafter disperse progeny to prosper in a new niche. Little is known about strategies displayed by Serratia marcescens to defeat immune responses and disseminate afterwards. Upon invasion of nonphagocytic cells, Serratia multiplies within autophagosome‐like vacuoles. These Serratia‐containing vacuoles (SeCV) circumvent progression into acidic/degradative compartments, avoiding elimination. In this work, we show that ShlA pore‐forming toxin (PFT) commands Serratia escape from invaded cells. While ShlA‐dependent, Ca2+ local increase was shown in SeCVs tight proximity, intracellular Ca2+ sequestration prevented Serratia exit. Accordingly, a Ca2+ surge rescued a ShlA‐deficient strain exit capacity, demonstrating that Ca2+ mobilization is essential for egress. As opposed to wild‐type‐SeCV, the mutant strain‐vacuole was wrapped by actin filaments, showing that ShlA expression rearranges host actin. Moreover, alteration of actin polymerization hindered wild‐type Serratia escape, while increased intracellular Ca2+ reorganized the mutant strain‐SeCV actin distribution, restoring wild‐type‐SeCV phenotype. Our results demonstrate that, by ShlA expression, Serratia triggers a Ca2+ signal that reshapes cytoskeleton dynamics and ends up pushing the SeCV load out of the cell, in an exocytic‐like process. These results disclose that PFTs can be engaged in allowing bacteria to exit without compromising host cell integrity. 相似文献
996.
PACAP and PAC1R are differentially expressed in motor cortex of amyotrophic lateral sclerosis patients and support survival of iPSC‐derived motor neurons 下载免费PDF全文
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Valentina Di Pietro Angela Maria Amorini Barbara Tavazzi Roberto Vagnozzi Ann Logan Giacomo Lazzarino Stefano Signoretti Giuseppe Lazzarino Antonio Belli 《Molecular medicine (Cambridge, Mass.)》2014,20(1):147-157
To characterize the molecular mechanisms of N-acetylaspartate (NAA) metabolism following traumatic brain injury (TBI), we measured the NAA, adenosine triphosphate (ATP) and adenosine diphosphate (ADP) concentrations and calculated the ATP/ADP ratio at different times from impact, concomitantly evaluating the gene and protein expressions controlling NAA homeostasis (the NAA synthesizing and degrading enzymes N-acetyltransferase 8-like and aspartoacylase, respectively) in rats receiving either mild or severe TBI. The reversible changes in NAA induced by mild TBI were due to a combination of transient mitochondrial malfunctioning with energy crisis (decrease in ATP and in the ATP/ADP ratio) and modulation in the gene and protein levels of N-acetyltransferase 8-like and increase of aspartoacylase levels. The irreversible decrease in NAA following severe TBI, was instead characterized by profound mitochondrial malfunctioning (constant 65% decrease of the ATP/ADP indicating permanent impairment of the mitochondrial phosphorylating capacity), dramatic repression of the N-acetyltransferase 8-like gene and concomitant remarkable increase in the aspartoacylase gene and protein levels. The mechanisms underlying changes in NAA homeostasis following graded TBI might be of note for possible new therapeutic approaches and will help in understanding the effects of repeat concussions occurring during particular periods of the complex NAA recovery process, coincident with the so called window of brain vulnerability. 相似文献