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941.
Plant allocation patterns may affect soil C and N storage due to differences in litter quality and the depth of plant C and
N inputs into the soil. We studied the dynamics of dual-labeled (13C/15N) Pinus ponderosa needles and fine roots placed at two soil depths (O and A horizon) in a temperate conifer forest soil during 2 y. Input of
C as fine roots resulted in much more C retained in soil (70.5 ± 2.2% of applied) compared with needle C (42.9 ± 1.3% of applied)
after 1.5 y. Needles showed faster mass loss, rates of soil 13CO2 efflux, and more 15N immobilized into microbial biomass than did fine roots. The larger proportion of labile C compounds initially present in
needles (17% more needle C was water soluble than in fine roots) likely contributed to its shorter C residence time and greater
degree of transformation in the soil. A double exponential decay function best described the rate of 13C loss, with a smaller initial pulse of C loss from fine roots (S1k1) and a slower decay rate of the recalcitrant C pool for fine roots (0.03 y−1) compared with (0.19 y−1) for needles. Soil 13C respiration, representing heterotrophic respiration of litter C, was much more seasonal from the O horizon than from the
A. However, offsetting seasonal patterns in 13C dynamics in the O horizon resulted in no net effect of soil depth on total 13C retention in the soil after 1.5 y for either litter. Almost 90% of applied litter N was retained in the soil after 1.5 y,
independent of litter quality or soil depth. Very small amounts of 13C or 15N (<3% of applied) moved to the horizon above or below the placement depth (i.e., O to A or A to O). Our results suggest that
plant allocation belowground to fine roots results in more C retained and less N mineralized compared with allocation aboveground
to needles, primarily due to litter quality differences. 相似文献
942.
Ito Y Abril ER Bethea NW McCuskey MK Cover C Jaeschke H McCuskey RS 《American journal of physiology. Gastrointestinal and liver physiology》2006,291(2):G211-G218
Neutrophil extravasation from sinusoids is a critical step for acute inflammatory tissue injury. However, the role of sinusoidal endothelial cells (SECs) in this process remains unclear. Matrix metalloproteinases (MMPs) have been shown to involve gap formation in SECs in several liver diseases. Therefore, the present study examined SEC modifications elicited by galactosamine (Gal)/endotoxin (ET). Treatment of male C3Heb/FeJ mice with Gal/ET or Gal/TNF caused the formation of numerous gaps in SECs at 4 h when no neutrophil extravasation occurred. Six hours after Gal/ET or Gal/TNF treatment, blood elements started to penetrate to the extrasinusoidal space through large gaps. Treatment with ET alone caused sinusoidal neutrophil accumulation but no gap formation, neutrophil extravasation, or hemorrhage. Gal/ET treatment increased hepatic MMP-2 and MMP-9 mRNA expression (6.7- and 11-fold, respectively). Pretreatment with 2-[(4-biphenylsulfonyl) amino]-3-phenyl-propionic acid, an MMP-2/MMP-9 inhibitor (5 mg/kg), minimized gap formation after Gal/ET and Gal/TNF treatment. The MMP inhibitor reduced injury only in the Gal/ET model mainly due to reduced TNF formation. The MMP inhibitor attenuated sinusoidal neutrophil accumulation at 6 h but failed to attenuate Gal/TNF-induced liver injury at 7 h due to excessive apoptosis. These results suggest that Gal/ET or Gal/TNF activates MMPs, which are responsible for SEC gap formation. Although the initial appearance of gap formation is independent of neutrophils, the gaps allow initial contact of neutrophils with damaged hepatocytes. In addition, MMP activation promotes neutrophil accumulation in sinusoids. 相似文献
943.
Dissociating the dual roles of apoptosis-inducing factor in maintaining mitochondrial structure and apoptosis 总被引:5,自引:0,他引:5
Cheung EC Joza N Steenaart NA McClellan KA Neuspiel M McNamara S MacLaurin JG Rippstein P Park DS Shore GC McBride HM Penninger JM Slack RS 《The EMBO journal》2006,25(17):4061-4073
The mitochondrial protein apoptosis-inducing factor (AIF) translocates to the nucleus and induces apoptosis. Recent studies, however, have indicated the importance of AIF for survival in mitochondria. In the absence of a means to dissociate these two functions, the precise roles of AIF remain unclear. Here, we dissociate these dual roles using mitochondrially anchored AIF that cannot be released during apoptosis. Forebrain-specific AIF null (tel. AifDelta) mice have defective cortical development and reduced neuronal survival due to defects in mitochondrial respiration. Mitochondria in AIF deficient neurons are fragmented with aberrant cristae, indicating a novel role of AIF in controlling mitochondrial structure. While tel. AifDelta Apaf1(-/-) neurons remain sensitive to DNA damage, mitochondrially anchored AIF expression in these cells significantly enhanced survival. AIF mutants that cannot translocate into nucleus failed to induce cell death. These results indicate that the proapoptotic role of AIF can be uncoupled from its physiological function. Cell death induced by AIF is through its proapoptotic activity once it is translocated to the nucleus, not due to the loss of AIF from the mitochondria. 相似文献
944.
In 2001 the European Register of Marine Species 1.0 was published (Costello et al. 2001 and http://erms.biol.soton.ac.uk/, and latterly: http://www.marbef.org/data/stats.php) [Costello MJ, Emblow C, White R, 2001. European register of marine species: a check list of the marine species in Europe and a bibliography of guides to their identification. Collection Patrimoines Naturels50, 463p.]. The lists of species (from fungi to mammals) were published as part of a European Union Concerted action project (funded by the European Union Marine Science and Technology (MAST) research programme) and the updated version (ERMS 2) is EU-funded through the Marine Biodiversity and Ecosystem Functioning (MARBEF) Framework project 6 Network of Excellence. Among these lists, a list of the fungi isolated and identified from coastal and marine ecosystems in Europe was included (Clipson et al. 2001) [Clipson NJW, Landy ET, Otte ML, 2001. Fungi. In@ Costelloe MJ, Emblow C, White R (eds), European register of marine species: a check-list of the marine species in Europe and a bibliography of guides to their identification. Collection Patrimoines Naturels50: 15-19.]. This article deals with the results of compiling a new taxonomically correct and complete list of all fungi that have been reported occurring in European marine waters. 相似文献
945.
BAL1 and BBAP are regulated by a gamma interferon-responsive bidirectional promoter and are overexpressed in diffuse large B-cell lymphomas with a prominent inflammatory infiltrate 总被引:1,自引:0,他引:1
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Juszczynski P Kutok JL Li C Mitra J Aguiar RC Shipp MA 《Molecular and cellular biology》2006,26(14):5348-5359
946.
Spermatozoa from diploid and tetraploid Pacific oysters (Crassostrea gigas) were examined after anisotonic fixation. Morphological anomalies, such as membrane rupture, detached tails, and the formation of tail vesicles (typically associated with damage attributable to procedures such as cryopreservation) were observed; the Mantel-Haenszel Chi-square test indicated a strong association between the anomalies and fixative osmolality (P<0.001). The present study also indicated that media in a range of 800 to 1,086 mOsm/kg could be assumed to be functionally isotonic to Pacific oysters, and osmolalities below or above this caused severe cell damage. For example, the maximum volume of flagella obtained after hypotonic fixation was approximately twice the volume of the flagella in isotonic fixation. Sperm cell flagellar volumes after hypertonic fixation (1,110 mOsm/kg) were 32% smaller than those in isotonic fixation, and sperm heads were 25% smaller. Although the damage associated with anisotonic fixation was evident in all parts of the sperm cells, the most vulnerable locations were the plasma membrane and flagellum motor apparatus. The formation of tail vesicles after hypotonic fixation was also examined. Because of water uptake, oyster sperm became swollen in hypotonic fixative, and bending or coiling of the axoneme within the tail vesicles led to the appearance of multiple axonemal structures in cross sections when observed by transmission electron microscopy. This phenomenon might be generally misinterpreted as the presence of double tails. This and other fixation artifacts can lead to the misinterpretation of damage caused by cryopreservation in ultrastructure studies of sperm of aquatic species, especially those in marine species.This work was supported in part by funding from the USDA-SBIR program, 4Cs Breeding Technologies, and the Louisiana Sea Grant College Program. 相似文献
947.
Aspartate transcarbamoylase (ATCase) and dihydroorotase (DHOase) catalyse the first two steps unique to pyrimidine synthesis.
In many bacteria they form non-covalently bonded complexes. There are two types of DHOase, type I and type II which share
a common ancestry. Type I is the more ancient form and is present in the complexes. In recently evolved bacteria the DHOase
is defective and its function has been replaced by a type II DHOase which is separate from the complex. Deinococcus radiophilus diverges early on the phylogenetic tree and so might be expected to have an active type I DHOase. Purification of the 500 kDa
ATCase–DHOase complex, by conventional techniques, showed it to possess an active DHOase. 相似文献
948.
949.
Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces 总被引:1,自引:0,他引:1
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Khodakaramian G Lissenden S Gust B Moir L Hoskisson PA Chater KF Smith MC 《Nucleic acids research》2006,34(3):e20
We report a system for the efficient removal of a marker flanked by two loxP sites in Streptomyces coelicolor, using a derivative of the temperate phage C31 that expresses Cre recombinase during a transient infection. As the test case for this recombinant phage (called Cre-phage), we present the construction of an in-frame deletion of a gene, pglW, required for phage growth limitation or Pgl in S.coelicolor. Cre-phage was also used for marker deletion in other strains of S.coelicolor. 相似文献
950.