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21.
Several reports in the past have dealt with the oxidation of cytochrome c added to suspensions of rat liver mitochondria. Yet, it is generally believed that the cytochrome cannot penetrate the outer membrane. Probably it has been assumed that the permeability of the outer membrane to cytochrome c is very low but finite, and that fast oxidation may be observed if time is allowed for sufficient penetration before initiation of electron flow. Here we show that this view is false. The main fraction of rat liver mitochondria, as isolated by conventional procedures, does not catalyse any significant oxidation of added cytochrome c, even after prolonged incubation. The observed appreciable oxidation of added cytochrome c is catalysed by a very small fraction (5-12%) of the mitochondria that apparently has a damaged outer membrane. Consequently, the turnover of cytochrome oxidase is very high in this fraction during oxidation of added cytochrome c. This finding readily explains why Moyle and Mitchell (e.g., FEBS Lett. 88 (1978) 268-272; 90 (1978) 361-365) have failed to observe proton translocation by cytochrome oxidase during oxidation of ferrocytochrome c added to rat liver mitochondria, which has been their main reason for rejecting the proton-pumping function of cytochrome oxidase. 相似文献
22.
Mutants of bacteriophage T4 that fail to induce nuclear disruption (ndd mutants) are unable to grow in the wild-type Escherichia coli strain CT447. This inhibition of the growth of ndd mutants occurs only in the presence of a large (ca. 80-megadalton) plasmid resident in CT447 cells. 相似文献
23.
Eleanor Leacock 《Dialectical Anthropology》1985,10(1-2):69-91
Conclusion Though relatively little direct attention has been given by Marxist anthropologists to a theory of individual behavior and thought in relation to societal processes, the above partial summary indicates the wealth of insight that is available for elaborating such a theory. In addition, there are of course significant developments in the field of psychology, notably the attention to activity as central to individual personality [], the renewed concern with levels of integration theory [], and the burgeoning interest in Vygotsky's writings on language, thought, and culture []. There are also the continuing attempts to locate Freud's positive contributions in a historical materialist frame [].Where, then, is psychological anthropology? As always, producing a richness of suggestive materials — such as those on varying conceptions of the self — but, as a glance at the pages of Ethos will show, unfortunately not engaged in fundamental theoretical innovation. By way of illustration, let me cite Spindler's The Making of Psychological Anthropology, a collection of articles by major figures, past and present. Spindler's introductory essay is thoughtful; always the teacher, he presents with consideration and modesty the history of the field, the disfavor into which it fell (in large part due to the methodlogical travesties of national character studies), and its stubborn persistence (following from the pervasive interest in the psychological dimension that has always characterized cultural anthropology). He summarizes ongoing problems as perceived from within the field, and as the major difficulty to be overcome pinpoints cultural overdeterminism and its inadequacy for explaining variations. The solution? Not the necessity of respecting history and focussing on how individuals variously understand and relate (according to their individual histories) to the established structures whereby particular societies produce, allocate, and consume basic goods, and how they variously respond to disjunctions in these structures even as they reproduce them. Instead Spindler writes, ... if we are to escape the double bind of our cultural overdeterminism, we are going to have to go beyond culture and even ecology, to biochemistry, to physiology and neurology, to genetics — to biology in the broadest sense of the term [].In closing, let me say to the reader, do not simply turn away in dismay. One must conclude that it is imperative to continue to build a solid alternative theory of relations among individual behaviors, individual understandings, cultural values, and societal processes, or, in other words, to replace a non-historical and essentially biological paradigm with a dialectical and materialist view of human action.Eleanor Leacock is Professor of Anthropology and Chair at the City College, City University of New York.
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24.
Metabolism of arachidonic acid and prostanoids in human endometrial stromal cells in monolayer culture 总被引:2,自引:0,他引:2
K Korte P C MacDonald J M Johnston J R Okita M L Casey 《Biochimica et biophysica acta》1983,752(3):423-433
In the present investigation, we compared the metabolism of arachidonic acid in human endometrial stromal cells maintained in monolayer culture with that in human decidual tissues. By gas-chromatographic analysis, the distribution of arachidonic acid in glycerophospholipids and in the neutral lipids of decidual tissues and stromal cells in culture was similar. After the addition of [14C]arachidonic acid to the culture medium, steady-state conditions with respect to radioactive labeling of the lipids of the cells were attained after 24 h, except for phosphatidylethanolamine and neutral lipids. The percentage distribution of [14C]arachidonic acid in the lipids of the cells in culture was as follows: phosphatidylcholine, 41%; phosphatidylserine, 5%; phosphatidylinositol, 19%; phosphatidylethanolamine, 22%; neutral lipids, 11%. This distribution of arachidonic acid among the lipids is similar to that in decidual tissue, except for that in phosphatidylethanolamine. The amount of radioactivity in phosphatidylethanolamine continued to increase up to 72 h whereas that in neutral lipids declined after a maximum amount was present at 4 h. In the cells in monolayer culture, [14C]prostaglandin E2 and [14C]prostaglandin F2 alpha were produced from [14C]arachidonic acid, as is true in superfused decidual tissue. The similarities in arachidonic acid metabolism in these cells to that in decidual tissue are supportive of the proposition that endometrial stromal cells in monolayer culture are an appropriate model for the study of the regulation of arachidonic acid release and prostaglandin formation by endometrium and decidua vera. 相似文献
25.
N,N′-Dicyclohexylcarbodiimide (DCCD) inhibits the activity of ubiquinol-cytochrome c reductase in the isolated and reconstitued mitochondrial cytochrome b-c1 complex. DCCD inhibits equally electron flow and proton translocation (i.e., the ratio is not affected) catalysed by the enzyme reconstituted into phospholipid vesicles. The inhibitory effects are accompanied by structural alterations in the polypeptide pattern of both isolated and reconstituted enzyme. Cross-linking was observed between subunits V (iron-sulfur protein) and VII, indicating that these polypeptides are in close proximity. A clear correlation was found between the kinetics of inhibition of enzymic activity and the cross-linking, suggesting that the two phenomena may be coupled. Binding of [14C]DCCD was also observed, to all subunits with the isolated enzyme and preferentially to cytochrome b with the reconstituted vesicles; in both cases, however, it was not correlated kinetically with the inhibition of the enzymic activity. 相似文献
26.
Structural and functional alterations induced in the mitochondrial cytochrome b-c1 complex by N,N'-dicyclohexylcarbodiimide 总被引:1,自引:0,他引:1
N,N'-Dicyclohexylcarbodiimide (DCCD) inhibits the activity of ubiquinol-cytochrome c reductase in the isolated and reconstituted mitochondrial cytochrome b-c1 complex. In proteoliposomes containing b-c1 complex DCCD inhibits equally electron flow and proton translocation catalyzed by the enzyme. In both isolated and reconstituted systems the inhibitory effect is accompanied by structural alterations in the polypeptide pattern of the enzyme consistent with cross-linking between subunits V and VII. The kinetics of inhibition of enzymic activity correlates with that of the cross-linking, suggesting that the two phenomena may be coupled. Binding of [14C] DCCD to both isolated and reconstituted enzyme was also observed, though it was not correlated kinetically with the inhibition. 相似文献
27.
Data concerning the temperature dependence of ouabain-sensitive (Na+ + K+)-activated ATPase have enabled estimates of the apparent activation energies of this process to be obtained. Arrhenius plots show a point of inflection at about 20 °; at higher temperatures the activation energy is about 13.5 kcal/mole while below this temperature the value increases to 28.5 kcal/mole. Storage at −5 ° or reduction in total cation concentration without alteration of the Na+:K+ ratio causes no significant change in these values, although the specific activity is markedly reduced. Reduction in the sodium concentration alone, however, increases the apparent activation energy at lower temperatures. These results support the hypothesis that two independent processes are involved in ATP hydrolysis, one operating above the critical temperature and one operating below this temperature. Storage, or reduction in the concentrations of both sodium and potassium ions, appears to reduce the number of functional ATPase units, without significantly altering the properties of those which can still hydrolyze ATP. Reduction in the sodium concentration alone, however, may also cause some inhibition of all units. This is more marked at lower temperatures, and may arise from competition by potassium for sodium-binding sites. 相似文献
28.
Involvement of recA and exr Genes in the In Vivo Inhibition of the recBC Nuclease 总被引:13,自引:5,他引:8
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Howard S. Marsden Ernest C. Pollard William Ginoza Eleanor P. Randall 《Journal of bacteriology》1974,118(2):465-470
When Escherichia coli cells are gamma irradiated they degrade their deoxyribonucleic acid (DNA). The DNA of previously gamma-irradiated T4 phage is also degraded in infected cells. The amount of degradation is not only dependent on the dose but also on the genotype of the cell. The amount of degradation is less in cells carrying a recB or a recC mutation, suggesting that most of the DNA degradation is due to the recB(+) and recC(+) gene product (exonuclease V). In some strains a previous dose of ultraviolet (UV) light followed by incubation renders the cells resistant to DNA degradation after gamma irradiation. We have shown this inhibition to take place for infecting T4 phage also. By using six strains of E. coli selected for mutations in the genes recA, exr (or lex), and uvrB, we have been able to show that the preliminary UV treatment produces no change in recA and exr cells for both endogenous DNA degradation and the degradation of infecting irradiated T4 phage DNA, i.e., inhibition was not detected in these strains. On the other hand, wild-type cells and strains carrying mutations of uvrB show inhibition in both types of experiments. Because the recA gene product and the exr(+) (lex(+)) gene product are necessary for the induction of prophage, it is possible that the phenomenon of inducible inhibition requires recA(+) and exr(+) presence. One interpretation of these results is that an inducible inhibitor may be controlled by the exr gene. 相似文献
29.
30.
Most carcinogens, including polycyclic aromatic hydrocarbons (PAH), require metabolic activation to produce the ultimate electrophilic species that bind covalently with cellular macromolecules to trigger the cancer process. Metabolic activation of PAH can be understood in terms of two main pathways: one-electron oxidation to yield reactive intermediate radical cations and monooxygenation to produce bay-region diol epoxides. The reason we have postulated that one-electron oxidation plays an important role in the activation of PAH derives from certain common characteristics of the radical cation chemistry of the most potent carcinogenic PAH. Two main features common to these PAH are: 1) a relatively low ionization potential, which allows easy metabolic removal of one electron, and 2) charge localization in the PAH radical cation that renders this intermediate specifically and efficiently reactive toward nucleophiles. Equally important, cytochrome P-450 and mammalian peroxidases catalyze one-electron oxidation. This mechanism plays a role in the binding of PAH to DNA. Chemical, biochemical and biological evidence will be presented supporting the important role of one-electron oxidation in the activation of PAH leading to initiation of cancer. 相似文献