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21.
22.
Biosynthesis of lysosomal hydrolases: their synthesis in bound polysomes and the role of co- and post-translational processing in determining their subcellular distribution 总被引:37,自引:20,他引:17
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By in vitro translation of mRNA’s isolated from free and membrane-bound polysomes, direct evidence was obtained for the synthesis of two lysosomal hydrolases, β-glucuronidase of the rat preputial gland and cathespin D of mouse spleen, on polysomes bound to rough endoplasmic reticulum (ER) membranes. When the mRNA’s for these two proteins were translated in the presence of microsomal membranes, the in vitro synthesized polypeptides were cotranslationally glycosylated and transferred into the microsomal lumen. Polypeptides synthesized in the absence of microsomal membranes were approximately 2,000 daltons larger than the respective unglycosylated microsomal polypeptides found after short times of labeling in cultured rat liver cells treated with tunicamycin. This strongly suggests that nascent chains of the lysosomal enzymes bear transient amino terminal signals which determine synthesis on bound polysomes and are removed during the cotranslational insertion of the polypeptides into the ER membranes. In the line of cultured rat liver cells used for this work, newly synthesized lysosomal hydrolases showed a dual destination; approximately 60 percent of the microsomal polypeptides detected after short times of labeling were subsequently processed proteolytically to lower molecular weight forms characteristic of the mature enzymes. The remainder was secreted from the cells without further proteolytic processing. As previously observed by other investigations in cultured fibroblasts (A. Gonzalez-Noriega, J.H. Grubbs, V. Talkad, and W.S. Sly, 1980, J Cell Biol. 85: 839-852; A. Hasilik and E.F. Neufeld, 1980, J. Biol. Chem., 255:4937-4945.) the lysosomotropic amine chloroquine prevented the proteolytic maturation of newly synthesized hydrolases and enhanced their section. In addition, unglycosylated hydrolases synthesized in cells treated with tunicamycin were exclusively exported from the cells without undergoing proteolytic processing. These results support the notions that modified sugar residues serve as sorting out signals which address the hydrolases to their lysosomal destination and that final proteolytic cleavage of hydrolase precursors take place within lysosome itself. Structural differences in the carbohydrate chains of intracellular and secreted precursors of cathespin D were detected from their differential sensitivity to digestion with endoglycosidases H and D. These observations suggest that the hydrolases exported into the medium follow the normal secretory route and that some of their oligosaccharides are subject to modifications known to affect many secretory glycoproteins during their passage through the Golgi apparatus. 相似文献
23.
Charles A. Eldridge 《Journal of theoretical biology》1980,87(2):385-400
A method for calculating time-dependent probabilities and rates of coagulation is given for particles on a bounded two-dimensional region. Equations are derived and solved for the random variations of displacements between two particles which undergo independent Brownian motion and bind irreversibly upon collision. The solution is used to calculate expected times required for collisions of particles. Comparison with earlier models shows that revised boundary values and initial conditions predictably lengthen required collision times by about a factor of four. Applicatons of the methodology are discussed. 相似文献
24.
Aedes aegypti mosquitoes were adversely affected by infections of the filarial worm Brugia pahangi. Infected mosquitoes flew significantly shorter distances and showed marked reductions in total flight time during 24-hr flight mill tests compared to uninfected controls. Total flight range and duration flown by infected mosquitoes remained relatively constant throughout the infection process, while control mosquitoes flew further and longer with increasing time after their blood meal. Furthermore, a significantly greater number of infected mosquitoes either died or were rendered incapable of flight. Of flying and nonflying mosquitoes with 6-day-old or older infections dissected for parasite burdens, the nonflying group contained significantly more worms. Results of this study indicate that developing filarial larvae within this mosquito vector reduce its ability to survive and to transmit its infection by reducing its flight capabilities. Conclusions from this study relate only to A. aegypti homozygous for the gene fm which is fully susceptible to this filarial parasite. 相似文献
25.
A developmental electron microscopic study of the parasitism of Rolylenchulus reniforrnis in resistant ''Peking'' and susceptible ''Lee'' soybeans was made during a 21-day period under controlled conditions. Within 2 days of inoculation, the nematode had penetrated the cortical cells to the endodermis where it inserted its stylet, secreted and initiated syncytial formation and cell hypertrophy. Syncytia primarily involved pericycle tissues and, to a lesser extent, xylem parenchyma and endodermis. When identifiable, the cell into which the nematode stylet was inserted to initiate syncytial development was endodermal. Susceptible tissues exhibited two basic phases of development during this infection period: (i) an initial phase represented by partial cell wail lysis and separation; and (ii) an anabolic phase, characterized by organelle proliferation and development accompanied by secondary wall deposits, which provided nutrition for sessile female development. The resistant or hypersensitive reaction (HR) lacked the anabolic phase found in the susceptible reaction, and was characterized by an extension and usually accelerated type of Iysis found in the first phase of the syncytial development. The HR was usually very evident 4 days after inoculation, and could be identified by an almost complete lysis of the cell walls and cytoplasm. The possibility that the initial cell of the developing syncytium or "prosyncyte" may influence a susceptible or resistant reaction is discussed. Successive stages of cell wall dissolution and the deposition of secondary cell walls are described. 相似文献
26.
Max Mallen‐Cooper Matthew A. Bowker Anita J. Antoninka David J. Eldridge 《Restoration Ecology》2020,28(Z2):S56-S66
Biocrusts are multifunctional communities that are increasingly being used to restore degraded or damaged ecosystems. Concurrently, restoration science is shifting away from the use of purely structural metrics, such as relative abundance, to more functional approaches. Although biocrust restoration technology is advancing, there is a lack of readily available information on how to monitor biocrust functioning and set appropriate restoration goals. We therefore compiled a selection of 22 functional indicators that can be used to monitor biocrust functions, such as CO2 exchange as an indicator of productivity or soil aggregate stability as a proxy for erosion resistance. We describe the functional importance of each indicator and the available protocols with which it may be measured. The majority of indicators can be measured as a functional trait of species by using patches of biocrust or cultures that contain only one species. Practitioners wishing to track the multifunctionality of an entire biocrust community would be advised to choose one indicator from each broad functional group (erosion resistance, nutrient accumulation, productivity, energy balance, hydrology), whereas a targeted approach would be more appropriate for projects with a key function of interest. Because predisturbance data are rarely available for biocrust functions, restoration goals can be based on a closely analogous site, literature values, or an expert elicitation process. Finally, we advocate for the establishment of a global trait database for biocrusts, which would reduce the damage resulting from repeated sampling, and provide a wealth of future research opportunities. 相似文献
27.
Jingyi Ding Samantha K. Travers Manuel Delgado‐Baquerizo David J. Eldridge 《Global Change Biology》2020,26(2):709-720
Woody plant encroachment is a major land management issue. Woody removal often aims to restore the original grassy ecosystem, but few studies have assessed the role of woody removal on ecosystem functions and biodiversity at global scales. We collected data from 140 global studies and evaluated how different woody plant removal methods affected biodiversity (plant and animal diversity) and ecosystem functions (plant production, hydrological function, soil carbon) across global rangelands. Our results indicate that the impact of removal is strongly context dependent, varying with the specific response variable, removal method, and traits of the target species. Over all treatments, woody plant removal increased grass biomass and total groundstorey diversity. Physical and chemical removal methods increased grass biomass and total groundstorey biomass (i.e., non‐woody plants, including grass biomass), but burning reduced animal diversity. The impact of different treatment methods declined with time since removal, particularly for total groundstorey biomass. Removing pyramid‐shaped woody plants increased total groundstorey biomass and hydrological function but reduced total groundstorey diversity. Environmental context (e.g., aridity and soil texture) indirectly controlled the effect of removal on biomass and biodiversity by influencing plant traits such as plant shape, allelopathic, or roots types. Our study demonstrates that a one‐size‐fits‐all approach to woody plant removal is not appropriate, and that consideration of woody plant identity, removal method, and environmental context is critical for optimizing removal outcomes. Applying this knowledge is fundamental for maintaining diverse and functional rangeland ecosystems as we move toward a drier and more variable climate. 相似文献
28.
Plant and Soil - Dryland biotic communities (plants and biocrusts) are known to maintain multiple functions (multifunctionality) and services (multiservices) that decline with overgrazing by... 相似文献
29.
Shrub encroachment, a global phenomenon with management implications, is examined in two papers in the current issue of Applied Vegetation Science. Barbosa da Silva et al. show that encroachment simplifies the herbaceous community, and Pittarello et al. illustrate how pastoral practices can restore encroached grasslands. While detrimental effects of shrub encroachment on grassland vegetation are often reported, we argue for a more holistic view when assessing this land‐cover change. 相似文献
30.
Species extinctions alter ecosystem services, and the magnitude of this impact is likely to change across environmental gradients. In Australia, soil‐disturbing mammals that are now considered ecologically extinct are thought to be important ecosystem engineers. Previous studies have demonstrated microsite‐level impacts of reintroduced soil‐disturbing mammals on soil functions, but effects are yet to be tested across larger scales. Further, it is unclear how impacts vary across environmental gradients and if the restoration potential of reintroductions changes with climate. We examined the effects of soil‐disturbing mammal reintroductions across a large rainfall gradient in Australia to test the hypothesis that ecosystem engineering effects on soil function depend on climate. We compared soil labile carbon, available nitrogen and the activity of four enzymes associated with nutrient cycling in three microsite types with and without soil‐disturbing mammals in five sites along a large rainfall gradient (166–870 mm). Soil enzyme activity was greatest in the presence of soil‐disturbing mammals and increased with rainfall, but soil available carbon and nitrogen varied across the gradient and among microsites. Microsite effects were often stronger than any effects of soil‐disturbing mammals, with soil beneath vegetated patches (shrubs and trees) having greater enzyme activity, carbon and nitrogen than bare soils. However, soil‐disturbing mammals homogenised nutrient distributions across microsites. The impacts of soil‐disturbing mammals on soil function previously detected at micro‐scales was detected at a landscape‐scale. However, the overall effects of soil‐disturbing mammals on soil functions varied with productivity (rainfall). The context of soil‐disturbing mammal reintroductions is thus likely to be critical in determining their effectiveness in restoring soil function. 相似文献