Cloning of the aspartate-β-semialdehyde dehydrogenase structural gene fromEscherichia coli K12

Transformation ofEscherichia coli strains with the recombinant plasmid, prepared by shot gun cloning with pBR322 and containing the geneasd, the structural gene of aspartate--semialdehyde dehydrogenase, results in an increase in specific activity of 65-fold of the enzyme in crude extracts. Approximately 60 mg of pure enzyme may be obtained from 10 g of transformed cells (wet weight) in a simplified purification procedure. The molecular weight, amino acid composition, and kinetic properties of the enzyme appear to be the same as previously reported, and the first 36 amino acids of the N-terminal sequence have been determined.     

Direct radioimmunoassay of estradiol-17β in defatted bovine milk

A radioimmunoassay was developed for rapid determination of estradiol-17β concentrations in unextracted defatted bovine milk. The assay was dependent on the use of a highly specific anti-estradiol-17β antiserum. Application of a formula to correct for the interference associated with individual milk samples and use of appropriate assay blanks facilitated interpolation on a buffer standard curve. The assay offered a high degree of sensitivity (0.6pg/ml milk) and a precision (within-assay coefficient of variation: 0.196; between-assay CV:0.191) comparable with contemporary extraction methods.     

Development of the ciliature of Tetrahymena thermophila: II. Spatial subdivision prior to cytokinesis

The cell surface of Tetrahymena thermophila is made up of an anterior region in which virtually all basal bodies of ciliary rows are ciliated, and the remainder in which ciliated and unciliated basal bodies are fairly irregularly interspersed. This pattern persists through interfission development until the stage of appearance of the equatorial ring of gaps in the ciliary rows that marks the fission zone. The ciliation pattern then becomes subdivided, in large part through the rapid ciliation of contiguous basal bodies located posterior to the fission zone. We interpret this process as a wave of ciliation of preexisting basal bodies that propagates posteriorly from the site of the fission zone. The location, extent, and timing of the ciliation process are the same in inverted as in normally oriented ciliary rows, in spite of the fact that in inverted rows the visible fission zone gap is tardily formed and the local configuration of ciliature around this gap is abnormal. The putative ciliation wave thus does not depend directly upon the local manifestations of the fission zone. However, in a cell-division-arrest mutant, cdaA1, analyzed under conditions in which formation of fission-zone gaps is permanently prevented in some ciliary rows but not in all, it is found that the ciliation pattern becomes subdivided in those ciliary rows that express fission-zone gaps and fails to become subdivided in neighboring rows that fail to manifest gaps. We interpret this combination of findings to indicate that a signal localized at the cell equator initiates a set of polarized developmental events that simultaneously create and demarcate two cellular fields within what was previously one. We further suggest that the characteristic tandem cell division pattern of ciliates is fundamentally a process of segmentation, which might involve mechanisms of gradient subdivision analogous to those taking place during segmentation of insects and other multicellular organisms.     

Microfilament bundles in antheridial nuclei of Achlya ambisexualis E 87

This is the first report of intranuclear microfilaments within gametangial nuclei of oömycetous fungi. Longitudinal sections of four to six microfilaments were frequently observed in meiotic antheridial nuclei of Achlya ambisexualis. Each microfilament measured approximately 7–10 nm in diameter. Spindle tubules (25 nm in diameter) were also observed within some of the nuclei possessing microfilaments.     

Citrulline metabolism in normal and citrullinemic human lymphocyte lines

Citrullinemia is one of the five aminoacidurias associated with the Krebs-Henseleit urea cycle. A long-term lymphocyte line (UM-21) derived from a patient with this disease and nine of ten clones of this line were found to have no activity for the enzyme argininosuccinate synthetase (AS), as demonstrated by their inability to grow in medium in which citrulline had been substituted for arginine, by their inability to incorporate arginine-C14 derived from citrulline-C14 into cellular protein, and by direct enzyme assay. One clone had normal or nearly normal argininosuccinate synthetase activity, as demonstrated by the same criteria. Nutritional "variants" able to grow logarithmically in medium containing citrulline were isolated from UM-21 and three clones. The apparent Kms of AS for citrulline in UM-21, the ten clones, the variant lines, and a normal line were measured and fell into three groups: AS in UM-21 and nine clones had no measurable apparent Km for citrulline; AS in the variant cells had apparent Kms for citrulline of approximately 20 mM; and AS in the normal cell line and one clone had apparent Kms for citrulline of 0.2 mM. The data suggest that the defect in the citrullinemic cell lines is due to a mutation in the structural gene coding for argininosuccinate synthetase.     

In Situ Lysis of φ29- and SPO1-Infected Bacillus subtilis

An improved method of in situ lysis of bacteriophage-infected Bacillus subtilis was developed and used to study 29 and SPO1 phage structures produced by individual cells.