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81.
Terminal Disinfection in Hospitals with Quaternary Ammonium Compounds by Use of a Spray-Fog Technique 下载免费PDF全文
Spray-fogging of hospital rooms with a quarternary ammonium disinfectant was found to be an effective means of reducing the number of detectable airborne and surface bacteria. The level of bacterial contamination in hospital rooms was determined before and after fogging by means of the gravitational fallout method, the petri dish swab technique, and volumetric air-sampling procedures. Rooms vacated by patients infected with staphylococci, streptococci, pseudomonads, and salmonellae were tested and found to be effectively decontaminated of most of the detectable organisms by the fogging procedure. 相似文献
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Summary The fine structure of the infundibular process of the hedgehog has been studied, using material fixed in osmium tetroxide and embedded in Vestopal W. The process resembles in general structure that of other mammals, but also shows features not previously described in other species.The nerve fibres contain a number of inclusions, namely: small vesicles, 300–500 Å in diameter; larger vesicles, up to 2000 Å in diameter, which contain a variable amount of osmiophilic material; hexagonal crystal-like bodies, approximately 1250 × 3000 Å in size, lying within a striated membranous sheath; and aggregate bodies made up of small electron dense granules, possibly derived from mitochondria.In addition complex multilamellate bodies occur in some nerve fibres, which apparently give rise to membranous vesicles. Pituicytes, of varying appearance, are often intimately related to the nerve fibres.The findings suggest that synthesis of material may occur in the distal part of the fibres of the hypothalamo-hypophysial tract.We are indebted to the Medical Research Council, who provided the electron microscope in the Department of Anatomy, University of Birmingham, which was used in this study. 相似文献
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Previously existing methods for determining the pH limits for the growth of microorganisms have involved (1), the setting up of individual cultures, each having a specific pH; (2), the pH gradient plate technique devised by Sacks (1956) in which a continuous pH gradient is established in a Petri dish by means of a buffer system; and (3), the pH gradient plate technique of Zak (unpublished), in which a continuous pH gradient is established by means of an electric current. The discontinuous pH gradient technique described here provides a convenient method of determining the maximum and minimum pH at which a microorganism can grow. The technique can be used aerobically or anaerobically, and has a precision of about ± 0.1 pH unit. Data are given for several yeasts and forSerratia marcescens. In all cases, the organisms tested continued to metabolize at pH values beyond those representing the limits for growth, sometimes by as much as 0.5 pH unit. The results suggest that pH limits are unsuitable criteria in microbial classification. 相似文献
87.
R. L. Holmes 《Cell and tissue research》1966,69(1):288-295
Summary The neurohypophysis of foetal macaque monkeys has been studied by optical and electron microscopy. Abundant elementary neurosecretory granules are present in the infundibular process by the middle third of gestation. Most of these are of variable electron density, and surrounded by a membrane larger than the granule, so that they appear haloed. A few fibres contain membrane-bounded electron-dense granules which show no halo. Inclusions of synaptic vesicle size are rare. The infundibular stem contains a few fibres with typical inclusions smaller than 1000 Å in diameter; the latter resemble inclusions in fibres of the median eminence.Dedicated to Professor W. Bargmann on his 60th birthday. 相似文献
88.
Holmes JR Alps BJ 《Canadian journal of comparative medicine and veterinary science》1967,31(4):92-102
Theoretical consideration has been given in two horses to the properties of the electric field created by the equine heart acting as a simple electric generator. The principles of the vectorial theory have been applied to test the validity of application of the dipole concept. The cardiac electric forces, althrough complex in the immediate region of the heart, appear at the body surface in a similar form to those arising from a relatively immobile, single equivalent dipole. The potential value of the technique of vectorcardiography in cardiological investigations is briefly discussed. 相似文献
89.
Differential expression of the receptors for epidermal growth factor and insulin in the developing human placenta 总被引:2,自引:0,他引:2
The detailed cellular distribution of epidermal growth factor (EGF) receptors and insulin receptors during the development of the human placenta was examined. We show that EGF receptors are expressed by villous cytotrophoblast cells in first trimester human placentae. However, where these cells proliferate to form extravillous cytotrophoblast cell columns, there is a dramatic decrease in EGF receptor expression. There is no such differential expression of insulin receptors on this cell population. In contrast, both EGF-and insulin-receptors are present throughout gestation on the microvillous membrane of the terminally differentiated and non-proliferative syncytiotrophoblast although, at term, EGF-but not insulin-receptors are also found on the basolateral membrane of this epithelium. We further show that EGF receptors isolated from first trimester and term human placentae have functional tyrosine kinase activities but differ in their extent of glycosylation. These results suggest that EGF receptors probably play several distinct functional roles in these epithelial cells depending on their proliferative capacity and differentiation status. 相似文献
90.
The nonlinear characteristics of soft gels and hydrated connective tissues in ultrafiltration 总被引:4,自引:0,他引:4
A one-dimensional ultrafiltration problem of fluid flow through a soft permeable tissue or gel under high pressure and compressive strain is solved. A finite deformation biphasic theory is used to model the behavior of the soft porous permeable solid matrix. This theory includes a Helmholtz free energy function which depends on the three principal invariants (I, II, III) of the right Cauchy-Green tensor and which satisfies the Baker-Ericksen inequalities on the principal stresses and strains. The dependence of the porosity phi f and the solidity phi s on deformation is deduced and a generalization of the exponential strain-dependent functional form for the permeability, k = k0 exp (M epsilon), of Lai and Mow (Biorheology 103, 111-123, 1980) is proposed. In this one-dimensional problem, we show that the dependence of the permeability on phi f, phi s, and III is equivalent to its dependence on hydration as proposed by Fatt and Goldstick (J. Colloid Sci. 20, 962-988, 1965). The exact solution of the ultrafiltration problem is derived and asymptotic and numerical methods are used to evaluate it. For high pressures and finite strains, the solution provides some surprising effects. The theory predicts that a material starting with a homogeneous porosity will have a strongly non-homogeneous porosity throughout the column during ultrafiltration. The resulting change in pore size through the filtration column may be very important in understanding its filtration characteristics. It is also found that there is a long delay time, up to 10 to 15 min, before the filtration velocity reaches an equilibrium. In filtration experiments where the rate of mass transport across the tissue or column of gel is important, sufficient time must be allowed for the steady state to be reached. 相似文献