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101.
102.
Alessandro Sisto Fabio Bonelli Felice Centini Christopher I. Fincham Edoardo Potier Edith Monteagudo Paolo Lombardi Federico Arcamone Cristina Goso Stefano Manzini Alessandro Giolitti Carlo A. Maggi Mariano Venanzi Basilio Pispisa 《Biopolymers》1995,36(4):511-524
In the course of a program aimed at synthesizing novel, potent NK-1 tachykinin receptor antagonists, we developed upon a bioactive model by comparing the low energy structures of a series of peptide and nonpeptide Substance P antagonists. The comparison was based on the super imposition of the aromatic rings, assuming that the rest of the molecule behaves predominantly as a template to arrange the key aromatic groups in the right spatial position. A series of 2-aminocyclohexane carboxylic acid analogues were then selected as the best templates for reproducing the postulated bioactive structure, leading to several pseudo-peptides with interesting biological activity. According to the molecular modeling, these compounds exhibit a neat parallel facing of the indolyl and naphthyl groups at about 3 Å distance. Ultraviolet absorption and steady state fluorescence measurements support this conclusion, showing a linear correlation between the spectral properties and the binding affinity of these analogues. Stacking of the indole ring with naphthalene gives rise to a complex characterized by a well-defined molar extinction coefficient. Consistently, steady state and lifetime fluorescence measurements suggest that the quenching process is ascribable to ground-state interactions between the chromophores. Implications of the π stacking propensity of aromatic groups in the biological activity of the compounds examined are briefly discussed. © 1995 John Wiley & Sons, Inc. 相似文献
103.
A whole-genome radiation hybrid panel for bovine gene mapping 总被引:15,自引:0,他引:15
104.
An infestation of a sooty tern colony on Bird Island, Seychelles, with the ixodid tick Amblovomma loculosum was studied during visits to the colony in 1993 to 1995. During the infestation, adult ticks were numerous during the birds' incubation, larvae were abundant when young chicks were present, and there was some evidence that nymphs were found when older chicks were present. The life cycle of the tick appeared closely synchronized with that of its host. Following a breeding failure of the sooty terns, caused by adverse weather in August-September 1994, few adult ticks were found during the birds' breeding season of 1995, suggesting that the absence of chicks late in the 1994 breeding season led to starvation of the ticks and their failure to survive their diapause until the onset of the birds' next breeding season. 相似文献
105.
Mart M. Cristina Bocaz Gastn Asp Estrella Roeckel Marlene 《Biotechnology Techniques》1997,11(3):163-166
The simultaneous determination by capillary ion electrophoresis of nitrate and nitrite in wastewaters with chloride concentrations of 15 to 23g/l is described. Chloride concentrations over 200mg/l hampered the determination; thus, lead, mercuric and silver acetate were used to precipitate chloride. Silver acetate added in 1.5 times the stoichiometric amount for AgCl formation gave the best results in terms of nitrate and nitrite peak resolution. 相似文献
106.
Christian Biémont Cristina Vieira Christine Hoogland Géraldine Cizeron Catherine Lœvenbruck Claude Arnault Jean-Pierre Carante 《Genetica》1997,100(1-3):161-166
To investigate the main forces controlling the containment of transposable elements (TE) in natural populations, we analyzed
the copia, mdg1, and 412 elements in various populations of Drosophila melanogaster and D. simulans. A lower proportion of
insertion sites on the X chromosome in comparison with the autosomes suggests that selection against the detrimental effects
of TE insertions is the major force containing TE copies in populations of Drosophila. This selection effect hypothesis is
strengthened by the absence of the negative correlation between recombination rate and TE copy number along the chromosomes,
which was expected under the alternative ectopic exchange model (selection against the deleterious rearrangements promoted
by recombination between TE insertions). A cline in 412 copy number in relation to latitude was observed among the natural
populations of D. simulans, with very high numbers existing in some local populations (around 60 copies in a sample from Canberra,
Australia). An apparent absence of selection effects in this Canberra sample and a value of transposition rate equal to 1–2
× 10-3 whatever the population and its copy number agree with the idea of recent but temporarily drastic TE movements in local populations.
The high values of transposition rate in D. simulans clearly disfavor the hypothesis that the low amount of transposable elements
in this species could result from a low transposition rate.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
107.
Abstract The tandem mini-exon gene repeat is an ideal diagnostic target for trypanosomatids because it includes sequences that are conserved absolutely coupled with regions of extreme variability. We have exploited these features and the polymerase chain reaction to differentiate Phytomonas strains isolated from phloem, fruit or latex of various host plants. While the transcribed regions are nearly identical, the intergenic sequences are variable in size and content (130–332 base pairs). The mini-exon genes of these phytomonads can therefore be distinguished from each other and from the corresponding genes in insect trypanosomes, with which they are oft confused. 相似文献
108.
Charles E. Sasser James G. Gosselink Erick M. Swenson D. Elaine Evers 《Wetlands Ecology and Management》1995,3(3):171-187
Floating marshes occur over 70% of the western Terrebonne Basin, Louisiana, USA, freshwater coastal wetlands. They are of several types: A free-floating thick-mat (45–60 cm) marsh dominated by Panicum hemitomon and Sagittaria lancifolia; a thick mat marsh dominated by Panicum hemitomon and Sagittaria lancifolia that floats part of the year, but whose vertical floating range is damped compared to adjacent water; and an irregularly-floating thin mat (< 30 cm) dominated by Eleocharis spp. in the spring and Ludwigia leptocarpa and Bidens laevis in the summer and fall. Floating mats must be almost entirely organic in order to be buoyant enough to float. The western Terrebonne wetlands receive large winter/spring supplies of suspended sediments from the Atchafalaya River. Even though sediment concentrations in the adjacent bayou are as high as 100 mg l–1, the Panicum hemitomon/Sagittaria lancifolia free-floating marsh probably receives no over-surface sediments since it floats continuously. The bulk density data of the damped-floating marsh, however, suggest some mineral sediment input, probably during winter when this marsh is submerged. These two types of floating marsh could not have developed in the present sediment regime of the Atchafalaya River, but as long as they remain floating can continue to exist. Thin floating mats are found in areas receiving the least sediment (<20 mg 1–1 suspended sediment concentration in adjacent bayous). This low sediment environment probably made possible their formation within the past 20 years. They may represent a transitional stage in mat succession from (1) existing thick-mat floating marsh to a degrading floating marsh, or (2) a floating marsh developing in shallow open water.Corresponding editor: D. Whigham 相似文献
109.
Meningococcal pilin: a glycoprotein substituted with digalactosyl 2,4-diacetamido-2,4,6-trideoxyhexose 总被引:19,自引:8,他引:11
Elaine Stimson Mumtaz Virji Katherine Makepeace Anne Dell Howard R. Morris Gail Payne Jon R. Saunders Michael P. Jennings Stephanie Barker Maria Panico Ian Blench E. Richard Moxon 《Molecular microbiology》1995,17(6):1201-1214
Neisseria meningitidis pili are filamentous protein structures that are essential adhesins in capsulate bacteria. Pili of adhesion variants of meningococcal strain C311 contain glycosyl residues on pilin (PilE), their major structural subunit. Despite the presence of three potential N -linked glycosylation sites, none appears to be occupied in these pilins. Instead, a novel O -linked trisaccharide substituent, not previously found as a constituent of glycoproteins, is present within a peptide spanning amino acid residues 45 to 73 of the PilE molecule. This structure contains a terminal 1-4-linked digalactose moiety covalently linked to a 2,4-diacetamido-2,4,6-trideoxyhexose sugar which is directly attached to pilin. Pilins derived from galactose epimerase ( galE ) mutants lack the digalactosyl moiety, but retain the diacetamidotrideoxyhexose substitution. Both parental (#3) pilins and those derived from a hyper-adherent variant (#16) contained identical sugar substitutions in this region of pilin, and galE mutants of #3 were similar to the parental phenotype in their adherence to host cells. These studies have confirmed our previous observations that meningococcal pili are glycosylated and provided the first structural evidence for the presence of covalently linked carbohydrate on pili. In addition, they have revealed a completely novel protein/saccharide linkage. 相似文献
110.