Microsomal-catalyzed hydroperoxide-dependent C-oxidation of amines

Organic hydroperoxides are capable of supporting the C-oxidation of several different amines in the presence of hepatic microsomes. Evidence is presented that indicates that microsomal cytochrome P-450 acts as the catalyst. Removal of the NADPH-cytochrome $\text{c}$ oxidoreductase or essential phospholipid from microsomes does not significantly affect the peroxidase activity. Of the amine substrates C-oxidized by organic hydroperoxides in the presence of microsomes, only aminopyrine and dimethylaniline are rapidly oxidized by hydroperoxides in the presence of catalase. The catalase-mediated reaction can also be distinguished from the microsomal-catalyzed reaction by the use of differential inhibitors.     

Enzyme des Stärkeumsatzes in Bündelscheiden- und Palisadenchloroplasten von Zea mays

Summary Isolated chloroplasts from the bundle sheath cells show considerable activity of the ADPG- and UDPG-pyrophosphorylase (EC 2.7.7.9), ADPG- and UDPG-transglucosylase (EC 2.4.1.21), and the starch phosphorylase (EC 2.4.1.1). In chloroplasts of the palisade cells, on the other hand, only the UDPG-pyrophosphorylase is remarkably active.     

Immunological studies in tropical splenomegaly syndrome in Uganda

An immunological evaluation carried out in eight patients with the tropical splenomegaly syndrome showed no evidence of impairment in cellular or humoral immunity, though raised levels of macroglobulins were noted in four patients. Hence gross immunological deficiency cannot be associated with the intense lymphoreticular proliferation observed in this disorder.     

Micro Radiometric Method for Study of Acid-insoluble Materials in Monolayer Cell Cultures

A simple radiometric procedure for study of acid-insoluble products synthesized in monolayer cell cultures is described. Cell cultures were produced directly on the bottom surface of scintillation vials or on glass cover slips (8 X 30 mm). The cells were labeled and extracted; the radioactivity was determined while the cells remained affixed to the glass surface upon which they were grown. This procedure enabled rapid investigations of certain biosynthetic processes to be carried out by using many individual cell cultures. The method was applied to an investigation of (3)H-thymidine incorporation induced by vaccinia virus in a 5-bromodeoxyuridine-resistant cell line. (14)C-labeling was evaluated as an alternate procedure for cell quantitation.     

Der Feinbau der sog. Kugelhaare der Fadenkanker (Opiliones,Nemastomatidae)

Zusammenfassung Der Hohlraum des Kugelhaares von Nemastoma bildet im basalen Teil Ausbuchtungen mit tubulären Strukturen, die mit 40–50 von proximal nach distal ziehenden Kanälen in Verbindung stehen.Nahe der Haarspitze befindet sich ein hohler Schirm mit zahlreichen Chaetoiden. Dicht unter ihm öffnen sich die Kanäle nach außen, und zwar derartig, daß ihr zentrad gelegener Teil der Wand den Schirmstiel bildet, während sich ihr peripherwärts gelegener Wandteil in einzelne Streben aufgliedert. Diese bilden nach Erreichen des Schirmbodens zwischen sich und dem Schirmstiel einen großen Hohlraum, der von einem klebrigen Sekret angefüllt und, zusammen mit dem Schirm, auch umhüllt wird.

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The fine structure of spezialized setae on the pedipalpi of nemastoma (Opiliones, Nemastomatidae)

Summary The cavity of the seta on the pedipalpi of Nemastoma shows protrusions with tubular structures, which are connected with 40–50 channels proceeding from proximal towards distal.Close by the tip of the seta there is a hollow umbrella with numerous secondary chetae. Bight underneath, each channel is widened in a way that the central part of the wall builds the stalk of the umbrella, whereas the more peripheral part of the wall is split in single struts. Both struts fused with the umbrella and its stalk form a large cavity. This cavity is filled with a viscid droplet and enveloped as well together with the umbrella. — The possible meaning of these structures is discussed.

Frl. A. Hennig bin ich für ihre gewissenhafte technische Mitarbeit sehr zu Dank verpflichtet.     

Uraninschwellen des Protoplasmas der BraunalgeStypocaulon scoparium

Ohne Zusammenfassung     

A physical map including a new class I gene (cda12) of the human major histocompatibility complex (A2/B13 haplotype) derived from a monosomy 6 mutant cell line

To avoid interpretative problems due to restriction fragment length polymorphisms, the monosomy 6 mutant cell line BM19.7 was employed to establish a molecular map of the human major histocompatibility (HLA) complex in the A2,B13,Bw4,DRw6,DRw52,DQw1,DPw2 haplotype. Results were obtained mainly by field-inversion gel electrophoresis and Southern blotting techniques. The map extends to 4800 kb and includes the HLA complex with a length of 4200 kb. Five HTF islands could be positioned on the map. The class I region has a size of about 2000 kb and includes nonclassical HLA class I genes, some of which must be localized within 200 kb telomeric of HLA-A. A new class I gene, cda12, distinct from HLA-A, HLA-B, or HLA-C, has been localized within 50 kb from HLA-A. The class I region contains a gap of about 500 kb, just telomeric of HLA-C, in which further class I genes could not be detected. The class II region has a size of 1000 kb, which is separated from the class I region by about 1200 kb. The 5' end of the HLA-B gene is situated centromeric, giving an orientation opposite to that of the TNFA and TNFB loci. The estimated length of the HLA complex correlates well with its size determined cytogenetically using mutant cell lines with interstitial deletions.     

Analysis of extracellular calcium and volume changes in the compound eye of the honeybee drone,Apis mellifera

Summary Superfused slices of drone retina were used for a quantitative analysis of light-induced changes in extracellular Ca²⁺ concentration ([Ca²⁺]_o) and extracellular space (ECS) volume. 20-ms light flashes elicited biphasic changes in [Ca²⁺]_o. For a saturating flash a brief, initial decrease was followed by a transient increase of 120±34 M. Long, dim steps of light (5 min) produced either a decrease or an increase in [Ca²⁺]_o depending strongly on the previous illumination. Brighter continuous lights caused the [Ca²⁺]_o to increase transiently by 1.4 mM to a peak from which it decayed to a plateau, up to 0.6 mM above the dark concentration.Light flashes (20 ms) caused a shrinkage in ECS volume not exceeding 4%. Thus, changes in [Ca²⁺]_o were almost completely due to Ca²⁺ fluxes between the ECS and adjacent cells. Continuous lights caused a shrinkage in ECS volume rarely exceeding 16%–20%. Thus, less than 15% of the measured Ca²⁺ changes could be attributed to shrinkage of the ECS. These data confirm that the ECS functions as a source and a sink for Ca²⁺ mobilized by light. For comparison, we also made a few measurements of changes in [Ca²⁺]_o in the retina ofCalliphora.Abbreviations [Ca ²⁺]_i intracellular free Ca²⁺ concentration - [Ca ²⁺]_o extracellular free Ca²⁺ concentration - ECS extracellular space - ER endoplasmic reticulum - TMA ⁺ tetramethylammonium ion