全文获取类型
收费全文 | 990篇 |
免费 | 80篇 |
专业分类
1070篇 |
出版年
2023年 | 6篇 |
2022年 | 10篇 |
2021年 | 14篇 |
2020年 | 10篇 |
2019年 | 11篇 |
2018年 | 10篇 |
2017年 | 17篇 |
2016年 | 28篇 |
2015年 | 45篇 |
2014年 | 39篇 |
2013年 | 52篇 |
2012年 | 57篇 |
2011年 | 71篇 |
2010年 | 50篇 |
2009年 | 45篇 |
2008年 | 49篇 |
2007年 | 49篇 |
2006年 | 43篇 |
2005年 | 40篇 |
2004年 | 47篇 |
2003年 | 36篇 |
2002年 | 27篇 |
2001年 | 22篇 |
2000年 | 26篇 |
1999年 | 18篇 |
1998年 | 8篇 |
1997年 | 16篇 |
1996年 | 14篇 |
1995年 | 13篇 |
1994年 | 10篇 |
1993年 | 8篇 |
1992年 | 15篇 |
1991年 | 13篇 |
1990年 | 12篇 |
1989年 | 16篇 |
1988年 | 12篇 |
1987年 | 6篇 |
1986年 | 9篇 |
1985年 | 9篇 |
1984年 | 10篇 |
1983年 | 8篇 |
1981年 | 4篇 |
1980年 | 4篇 |
1979年 | 8篇 |
1978年 | 3篇 |
1977年 | 10篇 |
1976年 | 5篇 |
1975年 | 5篇 |
1972年 | 3篇 |
1858年 | 3篇 |
排序方式: 共有1070条查询结果,搜索用时 8 毫秒
81.
Mass spectrometry-driven proteomics is increasingly relying on quantitative analyses for biological discoveries. As a result, different methods and algorithms have been developed to perform relative or absolute quantification based on mass spectrometry data. One of the most popular quantification methods are the so-called label-free approaches, which require no special sample processing, and can even be applied retroactively to existing data sets. Of these label-free methods, the MS/MS-based approaches are most often applied, mainly because of their inherent simplicity as compared to MS-based methods. The main application of these approaches is the determination of relative protein amounts between different samples, expressed as protein ratios. However, as we demonstrate here, there are some issues with the reproducibility across replicates of these protein ratio sets obtained from the various MS/MS-based label-free methods, indicating that the existing methods are not optimally robust. We therefore present two new methods (called RIBAR and xRIBAR) that use the available MS/MS data more effectively, achieving increased robustness. Both the accuracy and the precision of our novel methods are analyzed and compared to the existing methods to illustrate the increased robustness of our new methods over existing ones. 相似文献
82.
Sander Martens Stefan M. Wierda Mathijs Dun Michal de Vries Henderikus G. O. M. Smid 《PloS one》2015,10(2)
BackgroundFormal musical training is known to have positive effects on attentional and executive functioning, processing speed, and working memory. Consequently, one may expect to find differences in the dynamics of temporal attention between musicians and non-musicians. Here we address the question whether that is indeed the case, and whether any beneficial effects of musical training on temporal attention are modality specific or generalize across sensory modalities.Conclusion/SignificanceAB magnitude within one modality can generalize to another modality, but this turns out not to be the case for every individual. Formal musical training seems to have a domain-general, but modality-specific beneficial effect on selective attention. The results fit with the idea that a major source of attentional restriction as reflected in the AB lies in modality-specific, independent sensory systems rather than a central amodal system. The findings demonstrate that individual differences in AB magnitude can provide important information about the modular structure of human cognition. 相似文献
83.
84.
Abstract Myogenic contractions of the heart of the female blood-feeding insect, Rhodnius prolixus (Stål), are inhibited by crude extracts of testes applied directly to isolated dorsal vessels. Dorsal vessels were observed with a stereo microscope and heart beats timed with a stopwatch. In normal Rhodnius saline, hearts contract at 14.8 ± 7.1 beats per minute (n= 45). Crude extracts of the testes and the two male reproductive accessory organs (the opaque and transparent accessory glands) were prepared from previously frozen tissue by homogenizing 5–20 glands in a small glass homogenizer containing Rhodnius saline, centrifuging for 5 min at 2 000 g, and collecting the supernatant. Testes extract as low as 1.0 glands per mL inhibit contractions whereas crude extracts of the opaque or transparent accessory glands have no consistent effect. We refer to this cardiac inhibitor as rhodtestolin (Rhodnius testis inhibitory factor), and discuss its possible effects on the female during copulation. 相似文献
85.
Baart GJ Zomer B de Haan A van der Pol LA Beuvery EC Tramper J Martens DE 《Genome biology》2007,8(7):R136
Background
Neisseria meningitidis is a human pathogen that can infect diverse sites within the human host. The major diseases caused by N. meningitidis are responsible for death and disability, especially in young infants. In general, most of the recent work on N. meningitidis focuses on potential antigens and their functions, immunogenicity, and pathogenicity mechanisms. Very little work has been carried out on Neisseria primary metabolism over the past 25 years. 相似文献86.
Ming‐Dong Zhang Amit Zeisel André Calas Marc Landry Matthew Fuszard Sally L Shirran Robert Schnell Árpád Dobolyi Márk Oláh Lauren Spence Jan Mulder Henrik Martens Miklós Palkovits Mathias Uhlen Harald H Sitte Catherine H Botting Ludwig Wagner Sten Linnarsson Tibor Harkany 《The EMBO journal》2015,34(1):36-54
87.
88.
89.
Christoph Ott Henrik Martens Imam Hassouna Bárbara Oliveira Christian Erck Maria-Patapia Zafeiriou Ulla-Kaisa Peteri D?rte Hesse Simone Gerhart Bekir Altas Tekla Kolbow Herbert Stadler Hiroshi Kawabe Wolfram-Hubertus Zimmermann Klaus-Armin Nave Walter Schulz-Schaeffer Olaf Jahn Hannelore Ehrenreich 《Molecular medicine (Cambridge, Mass.)》2015,21(1):803-815
Erythropoietin (EPO) exerts potent neuroprotective, neuroregenerative and procognitive functions. However, unequivocal demonstration of erythropoietin receptor (EPOR) expression in brain cells has remained difficult since previously available anti-EPOR antibodies (EPOR-AB) were unspecific. We report here a new, highly specific, polyclonal rabbit EPOR-AB directed against different epitopes in the cytoplasmic tail of human and murine EPOR and its characterization by mass spectrometric analysis of immuno-precipitated endogenous EPOR, Western blotting, immunostaining and flow cytometry. Among others, we applied genetic strategies including overexpression, Lentivirus-mediated conditional knockout of EpoR and tagged proteins, both on cultured cells and tissue sections, as well as intracortical implantation of EPOR-transduced cells to verify specificity. We show examples of EPOR expression in neurons, oligodendroglia, astrocytes and microglia. Employing this new EPOR-AB with double-labeling strategies, we demonstrate membrane expression of EPOR as well as its localization in intracellular compartments such as the Golgi apparatus. Moreover, we show injury-induced expression of EPOR. In mice, a stereotactically applied stab wound to the motor cortex leads to distinct EpoR expression by reactive GFAP-expressing cells in the lesion vicinity. In a patient suffering from epilepsy, neurons and oligodendrocytes of the hippocampus strongly express EPOR. To conclude, this new analytical tool will allow neuroscientists to pinpoint EPOR expression in cells of the nervous system and to better understand its role in healthy conditions, including brain development, as well as under pathological circumstances, such as upregulation upon distress and injury. 相似文献
90.
The advent of algorithms for fragmentation spectrum-based label-free quantitative proteomics has enabled straightforward quantification of shotgun proteomic experiments. Despite the popularity of these approaches, few studies have been performed to assess their performance. We have therefore profiled the precision and the accuracy of three distinct relative label-free methods on both the protein and the proteome level. We derived our test data from two well-characterized publicly available quantitative data sets. 相似文献