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31.
Paavo N?rhi Maarit Middleton Nils Gustavsson Eija Hyv?nen Marja-Liisa Sutinen Raimo Sutinen 《Biogeochemistry》2011,102(1-3):239-249
To focus conservation efforts into forest areas with high biodiversity, more information is needed about soil-vegetation dependencies in Finnish Lapland. We studied understory vegetation and soil variables along a transect across a felsic?Cmafic lithological sequence in central Finnish Lapland. At 119 northern boreal forest sites, coverages of understory vegetation, several mineral soil chemical elements, soil electrical conductivity, pH, and dielectric permittivity, as a measure of soil volumetric water content, were measured. We found that soil Ca concentration and Ca:Al ratio were the main variables determining vegetation composition and diversity. Ca-rich soils were characterised by high electrical conductivity, pH, and Mg concentration, and by low concentration of Al, S, Zn, and low C:N ratio. Soil Ca concentration is a diagnostic measure of plant diversity as concentration higher than 100 mg kg?1 resulted in a considerable increase in plant diversity. Sites with Ca concentration this high were rare, and probably important in maintaining high biodiversity. The median soil Ca:Al ratio was only 0.02, suggesting, according to general theory, a considerable risk for aluminium stress. We found Geranium sylvaticum and Rubus saxatilis to be good indicators for Ca-rich regimes and high plant diversity. 相似文献
32.
Heikkilä E Ristola M Havana M Jones N Holthöfer H Lehtonen S 《The Biochemical journal》2011,435(3):619-628
Slit diaphragms are specialized junctions between glomerular epithelial cells (podocytes) that are crucial for glomerular ultrafiltration. The Ig superfamily members nephrin and Neph1 are essential components of the slit diaphragm, whereas the role of Neph1 homologue Neph3 in the slit diaphragm is unknown. In the present paper we show that Neph3 homodimerizes and heterodimerizes with nephrin and Neph1. We further investigated whether these interactions play a role in cell adhesion by using mouse L fibroblasts that lack endogenous cell-adhesion activity and found that Neph1 and Neph3 are able to induce cell adhesion alone, whereas nephrin needs to trans-interact with Neph1 or Neph3 in order to promote formation of cell-cell contacts. Tyrosine phosphorylation of nephrin was down-regulated after nephrin trans-interacted with either Neph1 or Neph3 leading to formation of cell-cell contacts. We further found that the expression of Neph3 was increased in nephrin-deficient mouse podocytes. The findings of the present paper show that nephrin and Neph1 or Neph3 trans-interactions promote cell-contact formation, suggesting that they may also function together in slit diaphragm assembly. 相似文献
33.
34.
Photosynthetic traits of Sphagnum and feather moss species in undrained,drained and rewetted boreal spruce swamp forests
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Tomáš Hájek Eija Juurola Rodney A. Chimner Lauri Mehtätalo Eeva‐Stiina Tuittila 《Ecology and evolution》2014,4(4):381-396
In restored peatlands, recovery of carbon assimilation by peat‐forming plants is a prerequisite for the recovery of ecosystem functioning. Restoration by rewetting may affect moss photosynthesis and respiration directly and/or through species successional turnover. To quantify the importance of the direct effects and the effects mediated by species change in boreal spruce swamp forests, we used a dual approach: (i) we measured successional changes in moss communities at 36 sites (nine undrained, nine drained, 18 rewetted) and (ii) photosynthetic properties of the dominant Sphagnum and feather mosses at nine of these sites (three undrained, three drained, three rewetted). Drainage and rewetting affected moss carbon assimilation mainly through species successional turnover. The species differed along a light‐adaptation gradient, which separated shade‐adapted feather mosses from Sphagnum mosses and Sphagnum girgensohnii from other Sphagna, and a productivity and moisture gradient, which separated Sphagnum riparium and Sphagnum girgensohnii from the less productive S. angustifolium, S. magellanicum and S. russowii. Undrained and drained sites harbored conservative, low‐production species: hummock‐Sphagna and feather mosses, respectively. Ditch creation and rewetting produced niches for species with opportunistic strategies and high carbon assimilation. The direct effects also caused higher photosynthetic productivity in ditches and in rewetted sites than in undrained and drained main sites. 相似文献
35.
High level of resistance to potato virus Y by expressing P1 sequence in antisense orientation in transgenic potato 总被引:2,自引:0,他引:2
36.
37.
Tanacetum vulgare (Tansy) was established in vitro on Murashige and Skoog (MS) medium supplemented with naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) using shoot tips and embryos. From petiole expiants 93% formed callus, and 27% produced shoots on MS medium containing 4.5 mg l-1 NAA and BAP. NAA alone induced root formation from leaf expiants. Up to 7 ×106 viable protoplasts were obtained by macerating 1 g of leaves in 0.5 % Macerozyme R-10, 1.0% Cellulase R10, and 1.0% Cellulysin. Cell division was observed 3–4 days after protoplast isolation at the optimum plating density of 0.2-0.4×106 cells ml-1. A total of 350 protoplast-derived calluses were produced on which nodules with meristematic zones developed. Roots regenerated on MS medium supplemented with BAP 3.0 mg 1-1, NAA 2.0 mg l-1, and 250 mg l-1 casein hydrolysate, however no shoots have been obtained yet.Abbreviations BAP
6-benzylaminopurine
- CH
casein enzymatic hydrolysate
- 2.4 D
dichlorophenoxyacetic acid
- FDA
fluorescein diacetate
- GA3
gibberellic acid
- IBA
indole butyric acid
- IPA
6-dimethylallylamino purine
- KIN
Kinetin
- MS
Murashige and Skoog medium
- NAA
-naphthaleneacetic acid 相似文献
38.
InDrosophila montana andD. littoralis (species of theD. virilis group), females use male courtship song in their mate choice in wild preferring males which produce short and dense sound
pulses (Aspi and Hoikkala, 1995). In the present study these song characters were found to be repeatable among overwintered
males. Male progenies of wild-caught flies reared in the laboratory, and inD. montana also the males collected in wild before overwintering, exhibited very little variation between males in these characters.
Contrary to pulse characters, pulse train characters measured forD. montana song varied significantly between laboratory-reared males. Our findings suggest that inD. montana andD. littoralis song characters playing a part in sexual selection in the wild are more condition dependent than song characters which are
not the direct targets of female choice. 相似文献
39.
Yuki Ohsaki Takeshi Kawai Yukichika Yoshikawa Jinglei Cheng Eija Jokitalo Toyoshi Fujimoto 《The Journal of cell biology》2016,212(1):29-38
Lipid droplets (LDs) in the nucleus of hepatocyte-derived cell lines were found to be associated with premyelocytic leukemia (PML) nuclear bodies (NBs) and type I nucleoplasmic reticulum (NR) or the extension of the inner nuclear membrane. Knockdown of PML isoform II (PML-II) caused a significant decrease in both nuclear LDs and type I NR, whereas overexpression of PML-II increased both. Notably, these effects were evident only in limited types of cells, in which a moderate number of nuclear LDs exist intrinsically, and PML-II was targeted not only at PML NBs, but also at the nuclear envelope, excluding lamins and SUN proteins. Knockdown of SUN proteins induced a significant increase in the type I NR and nuclear LDs, but these effects were cancelled by simultaneous knockdown of PML-II. Nuclear LDs harbored diacylglycerol O-acyltransferase 2 and CTP:phosphocholine cytidylyltransferase α and incorporated newly synthesized lipid esters. These results corroborated that PML-II plays a critical role in generating nuclear LDs in specific cell types. 相似文献
40.
Eveliina Munukka Petri Wiklund Tiina Partanen Sakari V?lim?ki Eija K. Laakkonen Maarit Lehti Pamela Fischer-Posovzsky Martin Wabitsch Sulin Cheng Pentti Huovinen Satu Pekkala 《PloS one》2016,11(4)
While the role of both elevated levels of circulating bacterial cell wall components and adipose tissue in hepatic fat accumulation has been recognized, it has not been considered that the bacterial components-recognizing adipose tissue receptors contribute to the hepatic fat content. In this study we found that the expression of adipose tissue bacterial flagellin (FLG)-recognizing Toll-like receptor (TLR) 5 associated with liver fat content (r = 0.699, p = 0.003) and insulin sensitivity (r = -0.529, p = 0.016) in humans (n = 23). No such associations were found for lipopolysaccharides (LPS)-recognizing TLR4. To study the underlying molecular mechanisms of these associations, human HepG2 hepatoma cells were exposed in vitro to the conditioned culture media derived from FLG or LPS-challenged human adipocytes. The adipocyte-mediated effects were also compared to the effects of direct HepG2 exposure to FLG and LPS. We found that the media derived from FLG-treated adipocytes stimulated fat accumulation in HepG2 cells, whereas either media derived from LPS-treated adipocytes or direct FLG or LPS exposure did not. This is likely due to that FLG-treatment of adipocytes increased lipolysis and secretion of glycerol, which is known to serve a substrate for triglyceride synthesis in hepatocytes. Similarly, only FLG-media significantly decreased insulin signaling-related Akt phosphorylation, IRS1 expression and mitochondrial respiratory chain ATP5A. In conclusion, our results suggest that the FLG-induced TLR5 activation in adipocytes increases glycerol secretion from adipocytes and decreases insulin signaling and mitochondrial functions, and increases fat accumulation in hepatocytes. These mechanisms could, at least partly, explain the adipose tissue TLR5 expression associated with liver fat content in humans. 相似文献