Polygyny and the breeding success of the great reed warblerAcrocephalus arundinaceus

Settlement date, mating status, and breeding success of individually marked great reed warblers, Acrocephalus arundinaceus, were studied during the 1980–84 breeding seasons in Kahokugata, Ishikawa Prefecture, Japan. Twenty-five per cent of the territorial males were polygynous, of which the majority were bigamous. The settling periods of both sexes were long, extending for 65 days in males and 49 days in females. About 80% of males and females settled in the first half of the settling period, and the settlement date of 28–54% males overlapped with that of females. Many of the late settlers were bachelors and the males which mated earlier tended to be polygynous. The timing of a male's settlement is important in acquiring mates. Fifty-five per cent of eggs laid were lost before fledging, mainly due to predation. The mean number of fledglings was 3.19 per primary female, 2.41 per secondary female, and 2.80 per monogamous female. Comparison of the number of fledglings of females which mated during the same period showed that the presence of another female in the same territory did not adversely affect the breeding success of either of the polygynous females. Polygynous males have the advantage of decreasing the risk of breeding failure under high predation pressure.     

Bromocriptine Mesylate Attenuates Amyotrophic Lateral Sclerosis: A Phase 2a,Randomized, Double-Blind,Placebo-Controlled Research in Japanese Patients

Objective

Bromocriptine mesylate (BRC), a dopamine D2 receptor agonist has been shown to confer neuroprotection, sustained motor function and slowed disease progression in mouse models of amyotrophic lateral sclerosis (ALS) Here we report a first in human trial in ALS.

Design

A multicenter, Riluzole add-on, randomized, double-blind, placebo controlled 102-week extension BRC clinical trial.

Methods

The trial was conducted between January 2009 and March 2012 on 36 Japanese ALS patients. A 12-week treatment with Riluzole observational period was followed by combined treatment (Riluzole + BRC; n = 29 or Riluzole + placebo; n = 7). The dosing commenced at 1.25 mg/day increasing in steps at two weeks intervals to a maximum of 15 mg/day. The efficacy of BRC was evaluated by comparing BRC and placebo groups upon completion of stepwise dosing at 14 weeks 2 points (1^st endpoint) and upon completion or discontinuation of the study (2^nd endpoint) of the dosing.

Results

Statistics analyses revealed a marginal BRC treatment efficacy with P≦20%to placebo by 1^st and 2^nd endpoint analysis. In the 1^st endpoint analysis, BRC group was significantly effective on the scores of ALSAQ40-communicaton (P = 1.2%), eating and drinking (P = 2.2%), ALSFRS-R total (P = 17.6%), grip strength (P = 19.8%) compared to the placebo group. In the 2^nd endpoint analysis, differences between the scores of Limb Norris Scale (P = 18.3%), ALSAQ40-communication (P = 11.9%), eating and drinking (P = 13.6%), and neck forward-bent test (P = 15.4%) of BRC group were detected between the two groups. There was no significant difference between the treatment groups for adverse events or serious drug reactions incidence.

Conclusions

BRC sustains motoneuronal function at least in part through BRC treatment. Further analysis involving a Phase 2b or 3 clinical trial is required but BRC currently shows promise for ALS treatment.

Trial Registration

UMIN Clinical Trials UMIN000008527     

Size-sex relationship and sexual dimorphism in JapaneseArisaema (Araceae)

The size-sex relationship and sexual differences of sixArisaema species native to Japan were investigated. The size-sex relationship showed almost the same pattern in all species. When the plant was small in size, the sex expression was male, and sex expression changed from male to female as the plant grew larger. Male ratios decreased rapidly around a critical size, but this critical size differed from one species to another. Sexual differences were detected in reproductive structures and behavior, although no difference was detected in vegetative structures. The stoutness, longevity and inner tissue of the scape showed remarkable differences between males and females, and this difference was represented most clearly as the size-weight relationship. Earlier initiation of flowering in males was also observed. No difference was found in resource allocation to reproductive structures between male and female plants at the flowering stage. However, a broad variation in the amount of resource allocation to reproductive structures was found at the fruiting stage in female individuals, which was attributed to differences in the setting rate of mature fruits.     

Histone H1 variant, H1R is involved in DNA damage response

In Saccharomyces cerevisiae, the linker histone HHO1 is involved in DNA repair. In higher eukaryotes, multiple variants of linker histone H1 exist but their involvement in the DNA damage response is unknown. To address this issue, we examined sensitivity to genotoxic agents in chicken DT40 cells lacking specific H1 variants. Among the six H1 variant mutants, only H1R(-/-) DT40 cells exhibited increased sensitivity to the alkylating agent methyl-methanesulfonate (MMS). The MMS sensitivity of H1R(-/-) cells was not enhanced by inactivation of Rad54. H1R(-/-) DT40 cells also exhibited: (i) a reduction in gene targeting efficiencies, (ii) impaired sister chromatid exchange, and (iii) an accumulation of IR-induced chromosomal aberrations at the G2 phase, all of which indicate the involvement of H1R in the Rad54-mediated homologous recombination (HR) pathway. The mobility of H1R but not H1L in the nucleus decreased after MMS treatment and the repair of double-stranded breaks generated by I-SceI was unaffected in H1R(-/-) cells, suggesting that H1R integrates into HR-mediated repair pathways at the chromosome structure level. Together, these findings provide the first genetic evidence that a specific H1 variant plays a unique and important role in the DNA damage response in vertebrates.     

Premature progression of anther early developmental programs accompanied by comprehensive alterations in transcription during high-temperature injury in barley plants

Kernel number per spike is one of the most important yield components of wheat. To map QTLs related to kernel number including spike length (SPL), spikelet number per spike (SPN), fertile spikelet number (FSPN), sterile spikelet number (SSPN) and compactness, and to characterize the inheritance modes of the QTLs and two-locus interactions, 136 recombinant inbred lines (RILs) derived from ‘Nanda2419’ x ‘Wangshuibai’ and an immortalized F₂ population (IF₂) generated by randomly permutated intermating of these RILs were investigated. QTL mapping made use of the previously constructed over 3300 cM linkage map of the RIL population. Three, five, two, two and six chromosome regions were identified, respectively, for their association with SPL, SPN, FSPN, SSPN, and compactness in at least two of the three environments examined. All compactness QTLs but one shared the respective intervals of QSpn.nau-5A and the SPL QTLs. Xcfd46–Xwmc702 interval on chromosome 7D was related to all traits but SSPN and had consistently the largest effects. The fact that not all the compactness QTL intervals were related to both SPL and SPN indicates that compactness is regulated by different mechanisms. Interval coincidence between QTLs of SPL and SPN and between QTLs of FSPN and SSPN was minimal. For all the traits, favorable alleles exist in both parents. Inheritance modes from additiveness to overdominance of the QTLs were revealed and two-locus interactions were detected, implying that the traits studied are under complex genetic control. The results could contribute to wheat yield improvement and better use of Wangshuibai and Nanda2419 the two special germplasms in wheat breeding program.     

FANCD2 influences replication fork processes and genome stability in response to clustered DSBs

Fanconi Anemia (FA) is a cancer predisposition syndrome and the factors defective in FA are involved in DNA replication, DNA damage repair and tumor suppression. Here, we show that FANCD2 is critical for genome stability maintenance in response to high-linear energy transfer (LET) radiation. We found that FANCD2 is monoubiquitinated and recruited to the sites of clustered DNA double-stranded breaks (DSBs) specifically in S/G2 cells after high-LET radiation. Further, FANCD2 facilitated the repair of clustered DSBs in S/G2 cells and proper progression of S-phase. Furthermore, lack of FANCD2 led to a reduced rate of replication fork progression and elevated levels of both replication fork stalling and new origin firing in response to high-LET radiation. Mechanistically, FANCD2 is required for correct recruitment of RPA2 and Rad51 to the sites of clustered DSBs and that is critical for proper processing of clustered DSBs. Significantly, FANCD2-decifient cells exhibited defective chromosome segregation, elevated levels of chromosomal aberrations, and anchorage-independent growth in response to high-LET radiation. These findings establish FANCD2 as a key factor in genome stability maintenance in response to high-LET radiation and as a promising target to improve cancer therapy.     

The Sg-1 glycosyltransferase locus regulates structural diversity of triterpenoid saponins of soybean

Triterpene saponins are a diverse group of biologically functional products in plants. Saponins usually are glycosylated, which gives rise to a wide diversity of structures and functions. In the group A saponins of soybean (Glycine max), differences in the terminal sugar species located on the C-22 sugar chain of an aglycone core, soyasapogenol A, were observed to be under genetic control. Further genetic analyses and mapping revealed that the structural diversity of glycosylation was determined by multiple alleles of a single locus, Sg-1, and led to identification of a UDP-sugar-dependent glycosyltransferase gene (Glyma07g38460). Although their sequences are highly similar and both glycosylate the nonacetylated saponin A0-αg, the Sg-1(a) allele encodes the xylosyltransferase UGT73F4, whereas Sg-1(b) encodes the glucosyltransferase UGT73F2. Homology models and site-directed mutagenesis analyses showed that Ser-138 in Sg-1(a) and Gly-138 in Sg-1(b) proteins are crucial residues for their respective sugar donor specificities. Transgenic complementation tests followed by recombinant enzyme assays in vitro demonstrated that sg-1(0) is a loss-of-function allele of Sg-1. Considering that the terminal sugar species in the group A saponins are responsible for the strong bitterness and astringent aftertastes of soybean seeds, our findings herein provide useful tools to improve commercial properties of soybean products.     

Association study on chromosome 20q11.21-13.13 locus and its contribution to type 2 diabetes susceptibility in Japanese

Several linkage studies have predicted that human chromosome 20q is closely related to type 2 diabetes, but there is no clear evidence that certain variant(s) or gene(s) have strong effects on the disease within this region. To examine disease susceptibility variant in Japanese, verified SNPs from the databases, with a minor allele frequency larger than 0.15, were selected at 10-kb intervals across a 19.31-Mb region (20q11.21-13.13), which contained 291 genes, including hepatocyte nuclear factor 4α (HNF4α). As a result, a total of 1,147 SNPs were genotyped with TaqMan assay using 1,818 Japanese samples. By searching for HNF4α as a representative disease-susceptible gene, no variants of HNF4α were strongly associated with disease. To identify other genetic variant related with disease, we designed an extensive two-stage association study (725 first and 1,093 second test samples). Although SNP1146 (rs220076) was selected as a landmark within the 19.31 Mb region, the magnitude of the nominal P value (P = 0.0023) was rather weak. Subsequently, a haplotype-based association study showed that two common haplotypes were weakly associated with disease. All of these tests resulted in non-significance after adjusting for Bonferroni’s correction and the false discovery rate to control for the impact of multiple testing. Contrary to the initial expectations, we could not conclude that certain SNPs had a major effect on this promising locus within the framework presented here. As a way to extend our observations, we emphasize the importance of a subsequent association study including replication and/or meta-analysis in multiple populations.Electronic supplementary material Supplementary material is available in the online version of this article at and is accessible for authorized users.     

Modulation of the Poly(ADP-ribosyl)ation Reaction via the Arabidopsis ADP-Ribose/NADH Pyrophosphohydrolase,AtNUDX7, Is Involved in the Response to Oxidative Stress

Here, we assessed modulation of the poly(ADP-ribosyl)ation (PAR) reaction by an Arabidopsis (Arabidopsis thaliana) ADP-ribose (Rib)/NADH pyrophosphohydrolase, AtNUDX7 (for Arabidopsis Nudix hydrolase 7), in AtNUDX7-overexpressed (Pro_35S:AtNUDX7) or AtNUDX7-disrupted (KO-nudx7) plants under normal conditions and oxidative stress caused by paraquat treatment. Levels of NADH and ADP-Rib were decreased in the Pro_35S:AtNUDX7 plants but increased in the KO-nudx7 plants under normal conditions and oxidative stress compared with the control plants, indicating that AtNUDX7 hydrolyzes both ADP-Rib and NADH as physiological substrates. The Pro_35S:AtNUDX7 and KO-nudx7 plants showed increased and decreased tolerance, respectively, to oxidative stress compared with the control plants. Levels of poly(ADP-Rib) in the Pro_35S:AtNUDX7 and KO-nudx7 plants were markedly higher and lower, respectively, than those in the control plants. Depletion of NAD⁺ and ATP resulting from the activation of the PAR reaction under oxidative stress was completely suppressed in the Pro_35S:AtNUDX7 plants. Accumulation of NAD⁺ and ATP was observed in the KO-nudx7- and 3-aminobenzamide-treated plants, in which the PAR reaction was suppressed. The expression levels of DNA repair factors, AtXRCC1 and AtXRCC2 (for x-ray repair cross-complementing factors 1 and 2), paralleled that of AtNUDX7 under both normal conditions and oxidative stress, although an inverse correlation was observed between the levels of AtXRCC3, AtRAD51 (for Escherichia coli RecA homolog), AtDMC1 (for disrupted meiotic cDNA), and AtMND1 (for meiotic nuclear divisions) and AtNUDX7. These findings suggest that AtNUDX7 controls the balance between NADH and NAD⁺ by NADH turnover under normal conditions. Under oxidative stress, AtNUDX7 serves to maintain NAD⁺ levels by supplying ATP via nucleotide recycling from free ADP-Rib molecules and thus regulates the defense mechanisms against oxidative DNA damage via modulation of the PAR reaction.Reactive oxygen species (ROS) are by-products of normal metabolic processes, including chloroplastic, mitochondrial, and plasma membrane-linked electron transport systems, in all aerobic organisms (Gutteridge and Halliwell, 1989). Although the production and destruction of ROS are in balance, the imposition of biotic and abiotic stressful conditions can give rise to excess concentrations of ROS, leading to an imbalance of production and scavenging mechanisms (Mittler, 2002; Mullineaux and Karpinski, 2002; Kroj et al., 2003; Mahalingam et al., 2003). Excess ROS, leading to oxidative stress, can damage organelles, oxidize proteins, nick DNA (single-base DNA damage), deplete antioxidant levels, and ultimately trigger cell death (Gutteridge and Halliwell, 1989). Recently, ROS have been recognized as important signaling molecules that control diverse signaling pathways involved in a variety of cellular responses such as programmed cell death, pathogen defense, and hormone signaling (Foyer and Noctor, 2005; Kwak et al., 2006; Torres et al., 2006). In addition, oxidative stress causes dramatic inhibition of the tricarboxylic acid cycle and large sectors of amino acid metabolism followed by backing up of glycolysis and diversion of carbon into the oxidative pentose phosphate pathway (Baxter et al., 2007). Therefore, organisms have developed efficient systems to keep ROS levels in check and repair damage from attack by ROS.Among various defense systems against attack by ROS, the poly(ADP-ribosyl)ation (PAR) of proteins by poly(ADP-Rib)polymerase (PARP), by which branched polymers of ADP-Rib are attached using β-NAD⁺ to a specific amino acid residue of an acceptor protein, is a posttranslational modification for responding early to DNA damage, such as single-strand DNA break and resealing, caused by oxidative stress and, thus, is crucial for genomic integrity and cell survival (Qin et al., 2008). PARP detects DNA strand breaks and converts the damage into intracellular signals that can activate DNA repair programs or cell death, according to the severity of the injury, via the PAR reaction of nuclear proteins involved in chromatin architecture and DNA metabolism and interacts with the x-ray repair cross complementing factor 1 (XRCC1), an adaptor protein that also has two interfaces with two important single-strand DNA break (SSB) repair (SSBR)/base excision repair (BER) enzymes: DNA ligase and DNA polymerase β (Caldecott et al., 1995, 1996; Kubota et al., 1996; Masson et al., 1998). DNA polymerase β fills the single nucleotide gap, preparing the strand for ligation by a complex of DNA ligase III and XRCC1 (Winters et al., 1999; Thompson and West, 2000). Thereby, the fast recruitment of SSBR/BER factors is archived in the site of the lesion. Modifications of proteins with poly(ADP-Rib) are reversed by poly(ADP-Rib) glycohydrolase (PARG), by which ADP-Rib polymers are hydrolyzed to free ADP-Rib, since incorrect signal transduction is caused by excessive accumulation of poly(ADP-Rib) modification (Davidovic et al., 2001). However, it has been reported that a massive PAR reaction results in the overconsumption of NAD⁺ and ATP and, ultimately, in energy depletion causing necrotic cell death (Ha and Snyder, 1999; Virág and Szabó, 2002; De Block et al., 2005).Nudix (for nucleoside diphosphates linked to some moiety X) hydrolases catalyze the hydrolysis of intact and oxidatively damaged nucleoside diphosphates and triphosphates, nucleotide sugars, coenzymes, dinucleoside polyphosphates, and RNA caps in various organisms such as bacteria, yeast, algae, nematodes, vertebrates, and plants (Bessman et al., 1996; Xu et al., 2004; Kraszewska, 2008). We have previously reported the characteristics of cytosolic Nudix hydrolases (AtNUDX1–AtNUDX11) in Arabidopsis (Arabidopsis thaliana; Ogawa et al., 2005). Among them, the recombinant AtNUDX7 showed high affinity for ADP-Rib and NADH as substrates in vitro, converting NADH to a reduced form of nicotinamide mononucleotide (NMNH) plus AMP and ADP-Rib to AMP plus Rib 5-P (Ogawa et al., 2005). AtNUDX7 was expressed more strongly in leaf than in stem and root. Therefore, the enzyme might be involved in nucleotide recycling relating to the metabolism of NADH and/or poly(ADP-Rib).Recent studies revealed that the actions of AtNUDX7 (At4g12720) are closely related to immune responses to pathogens. Knockout of AtNUDX7 (KO-nudx7) in Arabidopsis plants led to deleterious inference for cells, such as microscopic cell death, constitutive expression of pathogenesis-related genes, resistance to bacterial pathogens, and accumulation of NADH (Jambunathan and Mahalingam, 2006). Furthermore, AtNUDX7 exerted a negative regulatory effect on EDS1 signaling, which controls the activation of defenses and programmed cell death conditioned by intracellular Toll-related immune receptors that recognized specific pathogen effectors (Bartsch et al., 2006). More recently, Ge et al. (2007) reported that KO-nudx7 plants show heightened defense responses, which are both dependent on and independent of the accumulation of NPR1 and salicylic acid, to pathogenic attack. On the other hand, Adams-Phillips et al. (2008) reported that KO-nudx7 plants exhibit a reduced hypersensitive-response phenotype, although the growth of both virulent and avirulent pathogens is suppressed in the plants. These findings support the hypothesis that regulation of the metabolism of NADH and/or ADP-Rib by Nudix hydrolases is important for stress-related defense systems in higher plants. However, the direct actions of the enzymes on stress responses are not established yet.In this study, to assess the functions of Arabidopsis Nudix hydrolases having ADP-Rib and NADH pyrophosphohydrolase activities under normal conditions and oxidative stress, we analyzed the effect of the overexpression or disruption of AtNUDX7 on levels of ADP-Rib, NAD(H), and ATP as well as PAR activity and oxidative stress tolerance in Arabidopsis. The evidence presented here suggests that AtNUDX7 serves to balance between NADH and NAD⁺ by NADH turnover under normal conditions. In addition, AtNUDX7 functions in the maintenance of NAD⁺ levels by supplying ATP via nucleotide recycling from free ADP-Rib molecules and the modulation of the PAR reaction, thereby regulating the DNA repair pathways, in response to oxidative stress.