全文获取类型
收费全文 | 1553篇 |
免费 | 69篇 |
国内免费 | 1篇 |
出版年
2021年 | 13篇 |
2020年 | 6篇 |
2019年 | 8篇 |
2018年 | 16篇 |
2017年 | 9篇 |
2016年 | 20篇 |
2015年 | 33篇 |
2014年 | 41篇 |
2013年 | 101篇 |
2012年 | 82篇 |
2011年 | 80篇 |
2010年 | 43篇 |
2009年 | 48篇 |
2008年 | 84篇 |
2007年 | 82篇 |
2006年 | 78篇 |
2005年 | 73篇 |
2004年 | 76篇 |
2003年 | 95篇 |
2002年 | 67篇 |
2001年 | 44篇 |
2000年 | 32篇 |
1999年 | 33篇 |
1998年 | 21篇 |
1997年 | 17篇 |
1996年 | 24篇 |
1995年 | 14篇 |
1994年 | 23篇 |
1993年 | 25篇 |
1992年 | 38篇 |
1991年 | 37篇 |
1990年 | 26篇 |
1989年 | 25篇 |
1988年 | 21篇 |
1987年 | 16篇 |
1986年 | 22篇 |
1985年 | 10篇 |
1984年 | 15篇 |
1983年 | 10篇 |
1982年 | 11篇 |
1981年 | 14篇 |
1980年 | 12篇 |
1979年 | 18篇 |
1978年 | 7篇 |
1977年 | 13篇 |
1976年 | 10篇 |
1975年 | 3篇 |
1973年 | 4篇 |
1972年 | 3篇 |
1970年 | 3篇 |
排序方式: 共有1623条查询结果,搜索用时 21 毫秒
11.
Yokoyama Eiichi; Murakami Akio; Sakurai Hidehiro; Fujita Yoshihiko 《Plant & cell physiology》1991,32(6):827-834
Stability of thylakoid components under supra-high irradiancewas studied with the cyanophyte Synechocystis PCC 6714. Theactivity of overall photosynthesis was quickly inactivated (T1/2=20min) under supra-high irradiance (300 W m2, white light).In parallel with the inactivation of photosynthesis, QA in PSII was also inactivated. Both inactivations were acceleratedby chloramphenicol (CAP) addition. The reactivation of PS IIrequired weak irradiation and was suppressed by CAP. However,PS I measured as P700 was very stable. The level of PS I measuredas P700 was not significantly reduced by the irradiation for12 h even in the presence of CAP while the level of Cyt b559,component of PS II, was decreased markedly. The function ofPS I before and after supra-high irradiation with CAP was examinedby comparing sizes of P700 oxidation induced by a short flash,by a continuous light, and by determination of O2-and ferredoxin-reduction.No difference was observed in PS I actions before and afterthe irradiation treatment. These results indicate that the PSI complex is very tolerant of supra-high irradiation. However,the cells grown under supra-high irradiance contained much fewerPS I and PS II complexes than Cyt b6f complexes. Theformer levels were reduced to a half to one fourth of thosebefore growth while the level of Cyt b6f complex wasnot reduced so much. A possible mechanism for changes in thylakoidcomposition under supra-high irradiation was discussed. (Received February 16, 1991; Accepted June 12, 1991) 相似文献
12.
Mechanism for enterohepatic injury caused by circulatory disturbance of hepatic vessels in the rat 总被引:3,自引:0,他引:3
S Kawamoto S Tashiro Y Miyauchi M Inoue 《Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)》1991,198(1):629-635
We reported previously that a transient occlusion followed by reperfusion of the portal vein and the hepatic artery of the rat significantly decreased the transhepatic transport of a cholephilic compound, and that this decrease was prevented by pretreating animals with poly(styrene co-maleic acid butyl ester)-conjugated superoxide dismutase (SM-SOD). To elucidate the mechanism for oxidative injury of the liver and the site for the generation of superoxide radicals, the effect of a portosystemic bypass on the liver function was examined in the rat whose hepatic vessels were temporarily occluded. A portosystemic bypass inhibited the reperfusion-induced decrease in hepatic transport of bromosulfophthalein as effectively as did SM-SOD. Kinetic analysis using 125I-labeled albumin revealed that the permeability of the small intestine markedly increased after a transient occlusion. The increase in intestinal permeability was also inhibited either by SM-SOD or by the portosystemic bypass. Xanthine oxidase activity in portal plasma markedly increased during occlusion and reperfusion, while it remained within normal ranges in the bypassed group. Thus, superoxide radical, and/or its metabolite(s), might play a critical role in increasing the intestinal permeability and in the pathogenesis of reperfusion-induced liver injury. 相似文献
13.
Min-Hua Zong Toshiaku Fukui Takuo Kawamoto Atsuo Tanaka 《Applied microbiology and biotechnology》1991,36(1):40-43
Summary Bioconversion of three organosilicon compounds of different chain length between the silicon atom and the hydroxyl group (Me3Si(CH2)nOH, n = 1–3) by horse liver alcohol dehydrogenase (HLADH, EC 1.1.1.1.) was studied. Furthermore, the effect of the silicon atom on the HLADH-catalysed reaction was examined in comparison with the corresponding carbon compounds. HLADH could catalyse the dehydrogenation of trimethylsilyeethanol (n = 2) and trimethylsilylpropanol (n = 3). Trimethylsilylethanol was a better substrate than both its carbon analogue, 3,3-dimethylbutanol, and ethanol. The improved activity of HLADH on trimethylsilylethanol could be accounted for by a higher affinity toward HLADH and a lower activation energy of the reaction by HLADH than those of the carbon counterpart. These are derived from physical properties of the silicon atom, that is, the lower electronegativity and the bigger radius than those of the carbon atom. In contrast, HLADH showed no activity on trimethylsilylmethanol (n = 1), whereas it catalysed the dehydrogenation of the carbon analogue, 2,2-dimethylpropanol, fairly well. The reason for the inactivity of HLADH in the case of trimethylsilylmethanol based on the electric effect of the silicon atom is also discussed.
Offsprint requests to: A. Tanaka 相似文献
14.
K Kubota N Narita K Ohkubo S Shibanai K Nagae M Kubota N Odagiri T Kawamoto 《Acta anatomica》1988,133(3):200-208
In order to give a neuroanatomical evidence to the mechanism of shifting from sucking to biting, we investigated in prenatal, newborn and postnatal mice whether there is a time difference in the neurogenesis of the neurons relative to sucking and biting or in the histogenesis of their peripheral effector organs by the HRP labeling technique and electron microscopy. The results obtained are as follows. (1) At birth the facial motoneurons exceed the trigeminal motoneurons in cell area and development. (2) After birth, the trigeminal motoneurons grow rapidly and outstrip the growth of the facial motoneurons at the age of 6 days. (3) Thereafter, the cell area of both neuron types continues to increase gradually. (4) The initial sign of the alpha motor end plates is found in the orbicularis oris muscle innervated by the facial nerve in 17-day-old fetuses, while that of the trigeminal nerve is delayed in the masseter muscle of 18-day-old fetuses. (5) The initial sign of the muscle spindle appears with the sensory terminals in the masseter muscle of 17-day-old fetuses and the fundamental structure of the muscle spindle is formed in 4-day-old youngs. (6) Myelination of the facial nerve begins in 3-day-old youngs, while that of the trigeminal nerve becomes apparent in 4- or 5-day-old youngs. From these bases, it is obvious that the facial nerve elements related to sucking are firstly developed at birth and that the differentiation of the trigeminal nerve elements related to biting is rapidly accelerated after birth. 相似文献
15.
Kenji Hayasaka Yoshi Kawamoto Takayoshi Shotake Ken Nozawa 《Primates; journal of primatology》1986,27(1):103-114
We calculated the probability of paternity exclusion (P) in 6 troops of rhesus and Japanese macaques housed in open enclosures and 68 wild troops of Japanese, crab-eating, and toque
macaques using 33 genetic loci which encoded the blood protein variations detected by electrophoretic techniques. In the open
enclosures, especially of rhesus troops, we obtained a fairly high probability of paternity exclusion and succeeded in determining
the fathers of offspring. However, we found significant differences between the observed and calculated probabilities in most
of the troops. These differences were ascribed to a situation whereby the Hardy-Weinberg equilibrium had not been attained
in the troops and/or the numbers of variable loci were too small. In the wild troops of Japanese, crab-eating, and toque macaques,
the means ofP were 0.2274 (0.0192–0.5017), 0.4635 (0.1676–0.7151), and 0.7382 (0.6266–0.7954), respectively. We also estimated the number
of loci needed to determine the fathers of offspring with a probability of 0.8 assuming that ten males were present in the
troop. The estimated number was about 13.5 times, 5 times and 1.8 times the number of loci examined on average in the troops
of Japanese, crab-eating and toque macaques, respectively. This means that determination of most of the fathers of offspring
in wild troops of these macaques, even of toque macaques which have a rather high probability of paternity exclusion, is difficult
so long as we employ only electrophoretic techniques. 相似文献
16.
On the basis of an artificial defoliation experiment, a new growth model of soybean was formulated through a modification
of Rudd's (1980) model with regard to his equations for dry matter allocation. Compensatory growth for leaf damage was modelled
by a single process in which the dry matter allocation changes dynamically according to the severity of leaf damage. The sums
of squared differences between simulations and experimental soybean yields were much smaller in our modified model than in
Rudd's original model. The modified model gave a better simulation of yield loss due to defoliation that varied in time and
intensity. The relationship between various times and intensities of defoliation and yield loss was shown, which is essential
for establishing the dynamic economic injury level in IPM. 相似文献
17.
18.
T Kawamoto F Matsumura B V Madhukar D W Bombick 《Journal of biochemical toxicology》1989,4(3):173-182
TCDD was found to cause a marked inhibition of 125I-epidermal growth factor (EGF) binding to its receptor on the cell surface of XB mouse keratinizing epithelial cells (XB cells) cultured in vitro. The EC50 concentration was estimated to be on the order of 3 x 10(-11) M 24 hours after TCDD administration. As early as 12 hours after the addition of 10(-9) M of TCDD, XB cells showed signs of a decline in 125I-EGF binding levels. The level of such EGF receptor downregulation reached a maximum at 24 hours, continued until day 2, but completely recovered by day 3. This was accompanied by a rise in protein kinase activities, particularly those of the protein tyrosine kinases during the initial period of 6-24 hours. To test the hypothesis that the EGF receptors of the cells, by showing TCDD-induced symptoms of downregulation, actually are being activated and triggering EGF-like signals, we examined the effects of both TCDD and exogenously added EGF on cell morphology, colony formation degree of keratinization, the pattern of activation of protein kinases and de novo protein synthesis, and EGF receptor phosphorylation. Based on the similarity of cell responses to these between TCDD- and EGF-treated cells, we concluded that TCDD, directly or indirectly, causes activation of the EGF receptor. In contrast, 12-O-tetradencanoylphorbol-13-acetate (TPA), which is known to downregulate EGF receptors by blocking their protein tyrosine kinase, produced dissimilar end results. The balance of evidence support the notion that the action of TCDD in this cell line is tightly coupled to the activation of the EGF receptor and that one of the key consequences of such a biochemical change is that it signals these cells to commit to terminal differentiation. 相似文献
19.
Structural gene and complete amino acid sequence of Pseudomonas aeruginosa IFO 3455 elastase. 总被引:21,自引:8,他引:13
下载免费PDF全文
![点击此处可从《Journal of bacteriology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
J Fukushima S Yamamoto K Morihara Y Atsumi H Takeuchi S Kawamoto K Okuda 《Journal of bacteriology》1989,171(3):1698-1704
The DNA encoding the elastase of Pseudomonas aeruginosa IFO 3455 was cloned, and its complete nucleotide sequence was determined. When the cloned gene was ligated to pUC18, the Escherichia coli expression vector, bacteria carrying the gene exhibited high levels of both elastase activity and elastase antigens. The amino acid sequence, deduced from the nucleotide sequence, revealed that the mature elastase consisted of 301 amino acids with a relative molecular mass of 32,926 daltons. The amino acid composition predicted from the DNA sequence was quite similar to the chemically determined composition of purified elastase reported previously. We also observed nucleotide sequence encoding a signal peptide and "pro" sequence consisting of 197 amino acids upstream from the mature elastase protein gene. The amino acid sequence analysis revealed that both the N-terminal sequence of the purified elastase and the N-terminal side sequences of the C-terminal tryptic peptide as well as the internal lysyl peptide fragment were completely identical to the deduced amino acid sequences. The pattern of identity of amino acid sequences was quite evident in the regions that include structurally and functionally important residues of Bacillus subtilis thermolysin. 相似文献
20.
In situ phosphorylation of human platelet myosin heavy and light chains by protein kinase C 总被引:11,自引:0,他引:11
S Kawamoto A R Bengur J R Sellers R S Adelstein 《The Journal of biological chemistry》1989,264(4):2258-2265
Treatment of human platelets with 162 nM 12-O-tetradecanoylphorbol-13-acetate (TPA) resulted in phosphorylation of a number of peptides, including myosin heavy chain and the 20-kDa myosin light chain. The site phosphorylated on the myosin heavy chain was localized by two-dimensional peptide mapping to a serine residue(s) in a single major tryptic phosphopeptide. This phosphopeptide co-migrated with a tryptic peptide that was produced following in vitro phosphorylation of platelet myosin heavy chain using protein kinase C. The sites phosphorylated in the 20-kDa myosin light chain in intact cells were analyzed by two-dimensional mapping of tryptic peptides and found to correspond to Ser1 and Ser2 in the turkey gizzard myosin light chain. In vitro phosphorylation of purified human platelet myosin by protein kinase C showed that in addition to Ser1 and Ser2, a third site corresponding to Thr9 in turkey gizzard myosin light chain is also phosphorylated. The phosphorylatable myosin light chains from human platelets were found to consist of two major isoforms present in approximately equal amounts, but differing in their molecular weights and isoelectric points. A third, minor isoform was also visualized by two-dimensional gel electrophoresis. Following treatment with TPA, both the mono- and diphosphorylated forms of each isoform could be visualized, and the sites of phosphorylation were identified. The phosphate content rose from negligible amounts found prior to treatment with TPA to 1.2 mol of phosphate/mol of myosin light chain and 0.7 mol of phosphate/mol of myosin heavy chain following treatment. These results suggest that TPA mediates phosphorylation of both myosin light and heavy chains in intact platelets by activation of protein kinase C. 相似文献