全文获取类型
收费全文 | 235篇 |
免费 | 10篇 |
出版年
2023年 | 2篇 |
2022年 | 7篇 |
2021年 | 10篇 |
2020年 | 7篇 |
2019年 | 5篇 |
2018年 | 8篇 |
2017年 | 4篇 |
2016年 | 8篇 |
2015年 | 12篇 |
2014年 | 11篇 |
2013年 | 20篇 |
2012年 | 24篇 |
2011年 | 16篇 |
2010年 | 12篇 |
2009年 | 5篇 |
2008年 | 8篇 |
2007年 | 12篇 |
2006年 | 15篇 |
2005年 | 11篇 |
2004年 | 7篇 |
2003年 | 7篇 |
2002年 | 4篇 |
2001年 | 4篇 |
2000年 | 1篇 |
1999年 | 2篇 |
1996年 | 1篇 |
1994年 | 1篇 |
1993年 | 2篇 |
1992年 | 3篇 |
1987年 | 3篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1980年 | 2篇 |
1979年 | 1篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1976年 | 2篇 |
1975年 | 1篇 |
1974年 | 1篇 |
1972年 | 1篇 |
1969年 | 1篇 |
排序方式: 共有245条查询结果,搜索用时 16 毫秒
11.
Variation of toxigenic Vibrio cholerae O1 in the aquatic environment of Bangladesh and its correlation with the clinical strains 总被引:3,自引:0,他引:3
Islam MS Talukder KA Khan NH Mahmud ZH Rahman MZ Nair GB Siddique AK Yunus M Sack DA Sack RB Huq A Colwell RR 《Microbiology and immunology》2004,48(10):773-777
The diversity of toxigenic V. cholerae O1 in the aquatic environment of Bangladesh is not known. A total of 18 environmental and 18 clinical strains of toxigenic V. cholerae O1 were isolated simultaneously from four different geographical areas and tested for variation by the pulsed-field gel electrophoresis method. Environmental strains showed diversified profiles and one of the profiles was common to some environmental strains and most clinical strains. It appears that one clone has an advantage over others to cause disease. These findings suggest that the study of the molecular ecology of V. cholerae O1 in relation to its environmental reservoir is important in identifying virulent strains that cause disease. 相似文献
12.
Ribeiro FM Alves-Silva J Volknandt W Martins-Silva C Mahmud H Wilhelm A Gomez MV Rylett RJ Ferguson SS Prado VF Prado MA 《Journal of neurochemistry》2003,87(1):136-146
Synthesis of acetylcholine depends on the plasma membrane uptake of choline by a high affinity choline transporter (CHT1). Choline uptake is regulated by nerve impulses and trafficking of an intracellular pool of CHT1 to the plasma membrane may be important for this regulation. We have generated a hemagglutinin (HA) epitope tagged CHT1 to investigate the organelles involved with intracellular trafficking of this protein. Expression of CHT1-HA in HEK 293 cells establishes Na+-dependent, hemicholinium-3 sensitive high-affinity choline transport activity. Confocal microscopy reveals that CHT1-HA is found predominantly in intracellular organelles in three different cell lines. Importantly, CHT1-HA seems to be continuously cycling between the plasma membrane and endocytic organelles via a constitutive clathrin-mediated endocytic pathway. In a neuronal cell line, CHT1-HA colocalizes with the early endocytic marker green fluorescent protein (GFP)-Rab 5 and with two markers of synaptic-like vesicles, VAMP-myc and GFP-VAChT, suggesting that in cultured cells CHT1 is present mainly in organelles of endocytic origin. Subcellular fractionation and immunoisolation of organelles from rat brain indicate that CHT1 is present in synaptic vesicles. We propose that intracellular CHT1 can be recruited during stimulation to increase choline uptake in nerve terminals. 相似文献
13.
Mahmud T Bode HB Silakowski B Kroppenstedt RM Xu M Nordhoff S Höfle G Müller R 《The Journal of biological chemistry》2002,277(36):32768-32774
Short chain carboxylic acids are well known as the precursors of fatty acid and polyketide biosynthesis. Iso-fatty acids, which are important for the control of membrane fluidity, are formed from branched chain starter units (isovaleryl-CoA and isobutyryl-CoA), which in turn are derived from the degradation of leucine and valine, respectively. Branched chain carboxylic acids are also employed as starter molecules for the biosynthesis of secondary metabolites, e.g. the therapeutically important anthelmintic agent avermectin or the electron transport inhibitor myxothiazol. During our studies on myxothiazol biosynthesis in the myxobacterium, Stigmatella aurantiaca, we detected a novel biosynthetic route to isovaleric acid. After cloning and inactivation of the branched chain keto acid dehydrogenase complex, which is responsible for the degradation of branched chain amino acids, the strain is still able to produce iso-fatty acids and myxothiazol. Incorporation studies employing deuterated leucine show that it can only serve as precursor in the wild type strain but not in the esg mutant. Feeding experiments using (13)C-labeled precursors show that isovalerate is efficiently made from acetate, giving rise to a labeling pattern in myxothiazol that provides evidence for a novel branch of the mevalonate pathway involving the intermediate 3,3-dimethylacryloyl-CoA. 3,3-Dimethylacrylic acid was synthesized in deuterated form and fed to the esg mutant, resulting in strong incorporation into myxothiazol and iso-fatty acids. Similar experiments employing Myxococcus xanthus revealed that the discovered biosynthetic route described is present in other myxobacteria as well. 相似文献
14.
Akhirin regulates the proliferation and differentiation of neural stem cells in intact and injured mouse spinal cord 下载免费PDF全文
Felemban Athary Abdulhaleem M Xiaohong Song Rie Kawano Naohiro Uezono Ayako Ito Giasuddin Ahmed Mahmud Hossain Kinichi Nakashima Hideaki Tanaka Kunimasa Ohta 《Developmental neurobiology》2015,75(5):494-504
Although the central nervous system is considered a comparatively static tissue with limited cell turnover, cells with stem cell properties have been isolated from most neural tissues. The spinal cord ependymal cells show neural stem cell potential in vitro and in vivo in injured spinal cord. However, very little is known regarding the ependymal niche in the mouse spinal cord. We previously reported that a secreted factor, chick Akhirin, is expressed in the ciliary marginal zone of the eye, where it works as a heterophilic cell‐adhesion molecule. Here, we describe a new crucial function for mouse Akhirin (M‐AKH) in regulating the proliferation and differentiation of progenitors in the mouse spinal cord. During embryonic spinal cord development, M‐AKH is transiently expressed in the central canal ependymal cells, which possess latent neural stem cell properties. Targeted inactivation of the AKH gene in mice causes a reduction in the size of the spinal cord and decreases BrdU incorporation in the spinal cord. Remarkably, the expression patterns of ependymal niche molecules in AKH knockout (AKH?/?) mice are different from those of AKH+/+, both in vitro and in vivo. Furthermore, we provide evidence that AKH expression in the central canal is rapidly upregulated in the injured spinal cord. Taken together, these results indicate that M‐AKH plays a crucial role in mouse spinal cord formation by regulating the ependymal niche in the central canal. © 2014 Wiley Periodicals, Inc. Develop Neurobiol 75: 494–504, 2015 相似文献
15.
Jalal Rouhi Mohamad Hafiz Mamat C. H. Raymond Ooi Shahrom Mahmud Mohamad Rusop Mahmood 《PloS one》2015,10(4)
High-density and well-aligned ZnO–ZnS core–shell nanocone arrays were synthesized on fluorine-doped tin oxide glass substrate using a facile and cost-effective two-step approach. In this synthetic process, the ZnO nanocones act as the template and provide Zn2+ ions for the ZnS shell formation. The photoluminescence spectrum indicates remarkably enhanced luminescence intensity and a small redshift in the UV region, which can be associated with the strain caused by the lattice mismatch between ZnO and ZnS. The obtained diffuse reflectance spectra show that the nanocone-based heterostructure reduces the light reflection in a broad spectral range and is much more effective than the bare ZnO nanocone and nanorod structures. Dye-sensitized solar cells based on the heterostructure ZnO–ZnS nanocones are assembled, and high conversion efficiency (η) of approximately 4.07% is obtained. The η improvement can be attributed primarily to the morphology effect of ZnO nanocones on light-trapping and effectively passivating the interface surface recombination sites of ZnO nanocones by coating with a ZnS shell layer. 相似文献
16.
Sixteen new and one known metabolites 4-20 were obtained by incubation of tibolone (1) and hydroxytibolones (2 and 3) with various fungi. Their structures were elucidated by means of a homo and heteronuclear 2D NMR and by HREI-MS techniques. The relative stereochemistry was deduced by 2D NOESY experiment. Metabolites of tibolone (1) exhibited significant inhibitory activities against α-glucosidase and tyrosinase enzymes. Hydroxylations at C-6, C-10, C-11, C-15 positions and α,β-unsaturation at C-1/C-2, C-4/C-5 showed potent inhibitory activities against these enzymes. 相似文献
17.
18.
A low diversity Lower Ordovician trilobite assemblage is described from the middle part of the Lashkarak Formation at Simeh-Kuh, northwest of Damghan, northern Iran. Co-occurrence with conodonts of the lowermost Paroistodusproteus Biozone suggests an uppermost Tremadocian age. This assemblage includes four taxa, including one new genus and two new species, Damghanampyxginteri nov. gen. nov. sp. and Asaphellusfecundus nov. sp. The third diagnosable species from the formation is Taihungshaniamiqueli (Bergeron, 1894) which has previously been reported from southern France and the Turkish Taurides, suggesting north Gondwanan faunal affinities. 相似文献
19.
Casañola-Martín GM Marrero-Ponce Y Khan MT Ather A Sultan S Torrens F Rotondo R 《Bioorganic & medicinal chemistry》2007,15(3):1483-1503
20.
Rahmah Noordin Makoto Itoh Eisaku Kimura Rohana Abdul Rahman Balachandran Ravindran Rohela Mahmud Taniawati Supali Mirani Weerasooriya 《Filaria journal》2007,6(1):1-4