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61.
Two species of Parvicapsula were found in the kidney tubules and the urinary bladder of 2 pleuronectid fish from the northern Oresund, Denmark. The coelozoic, spherical, disporic trophozoites of both species are 10 to 12 pm in diameter. The myxospores of both species are elongate, asymmetrical and slightly curved, and have spherical polar capsules. Parvicapsula bicornis n. sp. (6-8 x 5-6 microm, polar capsule 2.5 microm in diameter) occurs in Pleuronectes platessa. The polar capsules of P. bicornis are arranged symmetrically on either side of the longitudinal axis and its spores differ from other species of Parvicapsula in having two 2-3 microm long posterior processes of different length. Parvicapsula limandae n. sp. (8-11 x 4-5 pm, polar capsule 1.6 microm in diameter) is found in Limanda limanda. The polar capsules are arranged along the longitudinal axis. It differs from Parvicapsula unicornis Kabata, 1962, recorded from L. limanda, in the arrangement of the polar capsules and in the absence of a posterior horn-like projection. The phylogenetic relationship between P. bicornis n. sp., P. limandae n. sp. and other Parvicapsula spp. was examined with their partial small subunit rDNA (SSU rDNA) sequences. P. limandae n. sp. and P. asymmetrica appear to be closely related, while P. bicornis n. sp. and P. minibicornis are the most divergent members of the genus. 相似文献
62.
Arne N. Linlkken Egil H. Wedul Kjell Langdal Olav Berge 《Zeitschrift fur angewandte Ichthyologie》2019,35(4):863-870
Preserving of fish species and populations is important whether it is for exploitation or just for conservation. Management of fisheries aim to maintain fishable stocks that are attractive to anglers, and different means are performed. In this study from the River South Rena in southeastern Norway, conducted during 1991–2005, the effects of supportive stocking of hatchery reared brown trout (Salmo trutta L.) from 1996, and bag limit (BL) and catch‐release (CR) practice for the target species brown trout, from 2002, were explored. Effects of supplemental brown trout stocking was not noticeable, except from one year following a year of exceptional high number of stocked fish, actually 41% of the catches, whereas in the following years this proportion remained constant about 10%, and the catches remained high in 2003 and 2004, mainly due to increased angling success rate after BL‐CR introduction. 相似文献
63.
64.
Teodora Ioana Flavia Paunas Kenneth Finne Sabine Leh Hans-Peter Marti Tom Eirik Mollnes Frode Berven Bjørn Egil Vikse 《Clinical proteomics》2017,14(1):30
Background
The clinical course of IgA nephropathy (IgAN) is variable and complement activation may predict prognosis. The present study investigated whether glomerular abundance of complement proteins associates with progression to end-stage renal disease (ESRD) in patients for whom prognosis could not be predicted based on clinical variables.Methods
Based on data from the Norwegian Kidney Biopsy Registry and the Norwegian Renal Registry, three groups were included: IgAN patients with (n = 9) or without (n = 16) progression to ESRD during 10 years, and controls (n = 15) with a normal kidney biopsy. IgAN patients had eGFR > 45 ml/min/1.73 m2 and non-nephrotic proteinuria at time of biopsy. Using stored formalin-fixed paraffin embedded kidney biopsy tissue, about 100 glomerular cross sections were microdissected for each patient. Samples were analyzed by liquid chromatography–tandem mass spectrometry and relative abundances of complement proteins were compared between groups.Results
Proteomic analyses quantified 2018 proteins, of which 28 proteins belong to the complement system. As compared to IgAN patients without progressive disease, glomeruli from patients with progressive IgAN had significantly higher abundance of components of the classical and the terminal complement pathways, and inhibitory factors such as Factor H and factor H related proteins. Abundance of complement proteins classified progressors from non-progressors with an area under ROC curve of 0.91 (p = 0.001). Clinical and morphological data were similar between the two patient groups and could not predict progressive IgAN.Conclusions
In conclusion, higher glomerular abundance of complement proteins was associated with a progressive clinical course in IgAN and are candidate biomarkers to predict prognosis.65.
Three primiparous dairy cows in early lactation with cannulas in rumen, duodenum and ileum were used in a 3×3 Latin square design to study effects of expander treatment of a barley-based concentrate. The concentrate was either pelleted at 75–80°C or expander treated at 125–130°C prior to pelleting. The diets consisted of 6.7 kg DM of grass silage and 10 kg DM of (1) 100% pelleted, (2) 50% pelleted and 50% expanded or (3) 100% expanded concentrate. The diets were offered as a mixed ration in four equal meals daily. Ruminal fermentation, bacterial N synthesis, duodenal, ileal and faecal flow of nutrients, and animal performance were monitored. Expander treatment numerically increased ruminal digestion of starch, which explained the observed increase in ruminal VFA concentration and the lowered ruminal pH (P<0.05). The proportion of butyrate in rumen liquid increased, whereas the proportion of propionate decreased in the expanded compared to the pelleted treatment (P<0.05). Expander treatment tended to increase rumen volume and rumen NDF pool size. Ruminal digestion of NDF was numerically lower in the expanded than in the pelleted treatment. No differences in bacterial N synthesis or efficiency of synthesis were observed among treatments. Expander treatment numerically increased the duodenal flow of non-ammonia N (NAN) and amino acid N (AAN), and seemed to increase the flow of non-ammonia non-bacterial N (NANBN) to the duodenum to a similar extent as was indicated by nylon bag studies. Milk production and milk fat and protein content were increased by the expander treatment (P<0.05), indicating that expander treatment increased the supply of nutrients for milk production. 相似文献
66.
Nutrient-saturated cultures of Skeletonema costatum were grownat 15C and 42 combinations of photon flux density (PFD) anddaylength. The growth rate increased with the daylength andPFD up to 460630 µE m2 s1 (maximum2.5 doublings day At 2000 µE m2 s1 the growthrate was reduced by 45%. The chlorophyll (chl) content of thecells and the rate of production of carbon per unit chlorophylland ambient light increased for declining light regimes as didcellular nitrogen and carbon. The N/C ratio, cellular phosphorusand ratios between in vivo fluorescence, with and without DCMU,and chlorophyll varied negligibly. The ATP/C ratio was linearlyrelated to the growth rate. The results were described mathematically.The chl/C ratio was low both in strong light and in marginallylow light, corresponding to low cellular chlorophyll and highcellular carbon, respectively. The observed increase in cellularnitrogen and carbon at shade adaptation probably represent anincrease in the size of internal stores of organic nitrogenand may imply that Skeletonema cells become enriched with organicnitrogen when staying in nitrate-rich subsurface layers, e.g.in or below a nutricline. However, close to zero growth in marginallight the cells become greatly enriched with respect to everymeasured factor. Such cells may be physiologically resting stageswhich may ensure survival during dark periods and promote rapiddevelopment during the initial phase of blooms. Cultures andnatural blooms of Skeleronema in the Trondheimsfjord exhibitvery similar patterns of variation. 相似文献
67.
Nutrient status of phytoplankton communities in Norwegian waters (marine, brackish, and fresh) as revealed by their chemical composition 总被引:4,自引:0,他引:4
Sakshaug Egil; Andresen Kjersti; Myklestad Sverre; Olsen Yngvar 《Journal of plankton research》1983,5(2):175-196
The chemical composition of nutrient-saturated cultures of Emilianiahuxleyi, Amphidinium carterae, and Staurastrum luetkemuelleriwas studied. The variation in chemical composition of naturalphytoplankton communities in the North Sea, the Trondheimsfjord,and a eutrophic lake was also studied. Nutrient status was evaluatedby measurement of the algal protein/carbohydrate, N/C, P/C,and N/P ratios. Tests for P-deficiency were carried out by measuringthe increase in ATP upon addition of phosphate. At saturationthe N/C ratio was {small tilde}0.14 in marine species and {smalltilde}0.05 in Staurastrum. Saturation P/C ratios (excludingpolyphosphates) were species-dependent, ranging from 0.017 (Skeletonema)to 0.006 (Amphidinium). Amphidinium and Staurastrum store polyphosphateswhen grown in P-rich media; true marine planktonic species donot. Natural communities tended to be close to nutrient saturationat low biomass densities and nutrient deficient at high densities.In the North Sea, nitrogen was clearly limiting. In waters offthe Møre coast and in the Trondheimsfjord, growth wasnearly balanced with respect to N and P at high salinities (>25)and clearly P-limited in brackish fjord waters. In dense communities,the N/P ratio was inversely related to salinity. Freshwatercommunities were clearly P-limited, but responses were dampenedwhen daphnia or whitefish were introduced, due to increasedexcretion of nutrients.
1Contribution No. 212, Trondheim Biological Station, N-7001Trondheim, Norway. 相似文献
68.
Trine Bjøro Olav Sand Bjørn Chr. Østberg Jan O. Gordeladze Peter Torjesen Kaare M. Gautvik Egil Haug 《Bioscience reports》1990,10(2):189-199
The effect of vasoactive intestinal peptide (VIP) on prolactin (PRL) secretion from pituitary cells is reviewed and compared to the effect of thyrotropin releasing hormone (TRH). These two peptides induced different secretion profiles from parafused lactotrophs in culture. TRH was found to increase PRL secretion within 4 s and induced a biphasic secretion pattern, while VIP induced a monophasic secretion pattern after a lag time of 45–60 s.The secretion profiles are compared to changes in adenylate cyclase activity, production of inositol polyphosphates, changes in intracellular calcium concentrations and changes in electrophysiological properties of the cell membrane.Abbreviations AC
adenylate cyclase
- DG
diacyglycerol
- GH
growth hormone
- GTP
guanosine trisphosphate
- Gi
GTP binding proteins that mediate inhibition of adenylate cyclase and that are pertussis toxin sensitive
- Gs
GTP binding protein that mediates stimulation of adenylate cyclase
- GH cells
clonal rat pituitary tumor cells producing PRL and/or growth hormone
- GH3 GH4C1 and GH4B6
subclones of GH cells
- PKA
protein kinase A
- PKC
protein kinase C
- PLC
phospholipase C
- PRL
prolactin
- TPA
12-O-tetradecanoyl phorbol 13-acetate
- TRH
thyrotropin releasing hormone
- VIP
vasoactive intestinal peptide 相似文献
69.
Nilsen N Nonstad U Khan N Knetter CF Akira S Sundan A Espevik T Lien E 《The Journal of biological chemistry》2004,279(38):39727-39735
Toll-like receptor 2 (TLR2) is a signaling receptor for a variety of microbial products, including bacterial lipoproteins and peptidoglycan, and is central in initiating immune responses toward Gram-positive bacteria, spirochetes, and mycobacteria. The mechanisms behind regulation of TLR2 protein expression are still not well understood. By using a newly developed monoclonal antibody against mouse TLR2, we detected TLR2 protein expression on macrophages, neutrophils, and dendritic cells. Endogenous macrophage TLR2 localized mostly to the cell membrane, with particular accumulation around phagosomes containing zymosan. Treatment of macrophages with the TLR2 antibody diminished cellular response to lipoproteins and down-regulated membrane TLR2. Marked up-regulation of surface TLR2 was observed on macrophages in response to whole bacteria, lipoproteins, lipopolysaccharide, poly(I-C) (double-stranded RNA), R848, and CpG DNA, and this up-regulation appeared to be a very sensitive marker for the presence of microbial products. Up-regulation of TLR2 in response to stimuli correlated with an increased response to secondary lipoprotein exposure following a low concentration of primary lipoprotein challenge. By comparison, exposure to a larger primary challenge induced a hyporeactive state. Most interestingly, lipopolysaccharide- and double-stranded RNA-induced up-regulation of surface TLR2 in macrophages was found to be MyD88-independent, whereas the up-regulation in response to lipoproteins, R848, and CpG DNA was absent in MyD88-deficient cells. We conclude that complex mechanisms regulate expression and signaling via TLR2. Up-regulation of TLR2 in the presence of low, yet clinically relevant amounts of microbial products may be an important mechanism by which the immune system boosts its response to a beginning infection. 相似文献
70.
We studied the variability of in vivo absorption coefficients and PSII‐scaled fluorescence excitation (fl‐ex) spectra of high light (HL) and low light (LL) acclimated cultures of 33 phytoplankton species that belonged to 13 different pigment groups (PGs) and 10 different phytoplankton classes. By scaling fl‐ex spectra to the corresponding absorption spectra by matching them in the 540–650 nm range, we obtained estimates for the fraction of total chl a that resided in PSII, the absorption of light by PSII, PSI, and photoprotective carotenoids. The in vivo red peak absorption maxima ranged from 673 to 679 nm, reflecting bonding of chl a to different pigment proteins. A simple approach is presented for quantifying intracellular self‐shading and evaluating the impact of photoacclimation on biooptical characteristics of the different PGs examined. In view of these results, parameters used in the calculation of oxygenic photosynthesis based on pulse‐amplitude‐modulated (PAM) and fast‐repetition‐rate (FRR) fluorometers are discussed, showing that the ratio between light available to PSII and total absorption, essential for the calculation of the oxygen release rate (using the PSII‐scaled fluorescence spectrum as a proxy) was dependent on species and photoacclimation state. Three subgroups of chromophytes exhibited 70%–80%, 60%–80%, and 50%–60% chl a in PSII‐LHCII; the two subgroups of chlorophytes, 70% or 80%; and cyanobacteria, only 12%. In contrast, the mean fraction for chromo‐ and chlorophytes of quanta absorbed by PSII was 73% in LL‐ and 55% in HL‐acclimated cells; thus, the corresponding ratios 0.55 and 0.73 might be used as correction factors adjusting for quanta absorbed by PSII for PAM and FRR measurements. 相似文献