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861.
KC Schuster R Goodacre JR Gapes M Young 《Journal of industrial microbiology & biotechnology》2001,27(5):314-321
Strain degeneration in solventogenic clostridia is a known problem in the technical acetone–butanol fermentation bioprocess,
especially in the continuous process mode. Clostridial strain degeneration was studied by Fourier transform infrared (FT-IR)
spectroscopy of the bacterial cells. Degenerative variant formation in two strains, Clostridium beijerinckii NCIMB 8052 and Clostridium species AA332, was detected spectroscopically. Colonies on solid media were sampled, or assayed directly in situ by IR microscopy. It has previously been shown that the distinctive acidogenic and solventogenic physiological phases of
Clostridium acetobutylicum in liquid medium can be discriminated by FT-IR spectroscopy. This was confirmed here for C. beijerinckii NCIMB 8052. The proportion of degenerate cells in a mixed population in liquid medium could be quantified, as the spectral
features change in different ways during the normal growth cycle of wild type organisms and degenerate variants in batch culture.
This opens a new perspective for physiology-based process monitoring and control, especially of the continuous acetone–butanol
fermentation.
Journal of Industrial Microbiology & Biotechnology (2001) 27, 314–321.
Received 06 October 2000/ Accepted in revised form 20 April 2001 相似文献
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In the search for natural enemies of Halogeton glomeratus (Bieb.)C. A. Meyer, a noxious weed introduced into the United States,studies were made, in 1959-61, on the distribution of H. sativus,a closely related species, occurring within Morocco. This plantis found in the Rif Mountains in the north, throughout the MoulouyaRiver Valley, along the Ziz River Valley, scattered along thesouthern slopes of the High Atlas Mountains as far west as Amerzgane,with an apparently discontinuous area of distribution on thenorthern slopes of the High Atlas in the vicinity of Asni. 相似文献
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SYNOPSIS. Four new genera of Foraminiferida are described. Phlegeria , type species P. hyalina sp. nov. and Tomaculoides , type species T. lucidum sp. nov. belong to the family Glandulinidae, and both were obtained from the Gulf of Mexico. Francesita , type species Virgulina ? advena Cusbman is found in the North Atlantic and the Gulf of Mexico, and belongs to the Caucasinidae. Montfortella , type species M. bramlettei sp. nov. occurs in Pleistocene strata and is living on the coast of California, and belongs to the Cibicididae. 相似文献