Clusterin/apolipoprotein J in human aging and cancer

Clusterin/Apolipoprotein J (ApoJ) is a heterodimeric highly conserved secreted glycoprotein being expressed in a wide variety of tissues and found in all human fluids. Despite being cloned since 1989, no genuine function has been attributed to ApoJ so far. The protein has been reportedly implicated in several diverse physiological processes such as sperm maturation, lipid transportation, complement inhibition, tissue remodeling, membrane recycling, cell-cell and cell-substratum interactions, stabilization of stressed proteins in a folding-competent state and promotion or inhibition of apoptosis. ApoJ gene is differentially regulated by cytokines, growth factors and stress-inducing agents, while another defining prominent and intriguing ApoJ feature is its upregulation in many severe physiological disturbances states and in several neurodegenerative conditions mostly related to advanced aging. Moreover, ApoJ accumulates during the viable growth arrested cellular state of senescence, that is thought to contribute to aging and to tumorigenesis suppression; paradoxically ApoJ is also upregulated in several cases of in vivo cancer progression and tumor formation. This review focuses on the reported data related to ApoJ cell-type and signal specific regulation, function and site of action in normal and cancer cells. We discuss the role of ApoJ during cellular senescence and tumorigenesis, especially under the light of the recently demonstrated various ApoJ intracellular protein forms and their interaction with molecules involved in signal transduction and DNA repair, raising the possibility that its overexpression during cellular senescence might cause a predisposition to cancer.     

GIST suture-line recurrence at a gastrojejunal anastomosis 8 years after gastrectomy: can GIST ever be described as truly benign? A case report

Background

A synovial sarcoma (SS) is an aggressive soft tissue tumor that classically occurs in the extremities near, but rarely within large joints, in young adults. Variable symptoms and clinical manifestations may be encountered and a definite diagnosis should depend on pathological results. This poses certain difficulties in arriving at a prompt diagnosis and appropriate treatment.

Case presentation

We report the case of a 68-year-old woman patient who presented an inguinal mass with swelling and pain in the right lower limb. She underwent surgery, and later received systematic intravenous chemotherapy. The pathological studies, especially the specific chromosomal translocation of a t(X;18) (p11.2;q11.2), confirmed the diagnosis as a synovial sarcoma. To the best of our knowledge, this is the first report of a monophasic synovial sarcoma in the inguinal region.

Conclusion

Besides making the readership aware of the rarity of location and age of this present case, this report distinctly highlights the great value of a molecular analysis of an SYT associated genetic alteration in the diagnosis of synovial sarcoma occurring at rare sites especially when immunochemical results are equivocal.     

A crosstalk of auxin and GA during tuber development

Several hormones have been studied for their effect on tuber initiation and development. Until recently, the hormone with the most prominent role in tuber initiation was attributed to GA. Genes involved in GA degradation do exhibit an upregulated profile during early stages of tuber development, leading to a rapid decrease of active GA content, thereby facilitating stolon-tip swelling. While GA is known to be involved in shoot and stolon elongation, the development of the new tuberorgan requires changes in meristem identity and the reorientation ofthe plane of cell division. In other developmental processes, such as embryo patterning, flower development and lateral root initiation auxin plays a key role. Recent evidence on the involvement of auxin in tuber formation was providedby the measurement of auxin content in swelling stolons. Auxin content in the stolon tips increased several fold prior to tuber swelling. In vitro tuberisation experiments with auxin applications support the role of auxin during tuber initiation. Taken together, it is becoming clear that the initiation and induction of tubers in potato is a developmental process that appears to be regulated by a crosstalk between GA and auxin.     

Blood viscosity modelling: influence of aggregate network dynamics under transient conditions

This paper reports on a theoretical examination of the hypothesis that red blood cell network characteristics influence the mechanical properties of the fluid. For this purpose a newly developed energy-rate based blood viscosity model, which incorporates network dynamics, was used to predict the transient behaviour of blood viscosity (steady-state results of this model have been reported in Biorheology 46 (2009), 487-508). The main network characteristic examined in the present work was the inter-aggregate branch size and its relationship to the evolving aggregates. Branch size was used to define a network integrity index that accounted for the strength of the developed network. For the development and validation of the model, experiments performed with an optical shearing microscope, with different step-changes in shear rate, were utilised, as well as viscosity measurements under similar flow conditions performed in a double wall Couette instrument. The experimental data were compared with the response of the model, which incorporated the network integrity index. The results suggest that network characteristics may influence the viscosity of blood at low shear rates and exhibit good agreement with experimental observations.     

Phenotypic and Molecular Biological Characterization of Cyanobacteria from Marble Surfaces of Treated and Untreated Sites of Propylaea (Acropolis,Athens)

Cyanobacteria cause aesthetic damage to marble surfaces and in particular their endolithic mode of life contributes to the breakdown of rock crystalline structures. The aim of this work was to estimate, with both phenotypical and molecular approach, the composition of cyanobacterial communities on the Propylaea marbles of the Acropolis Monuments. The two selected sampling sites were treated and untreated with a commercial biocide in order to estimate its effect on the cyanobacterial diversity. Our study revealed that in both sampling sites were present 13 phenotypes and 10 phylotypes and that the cyanobacterial taxa were considerably lower in the treated site.     

Comparative performance evaluation of a flat detector and an image intensifier angiographic system both used for interventional cardiology procedures in adult and pediatric patients

PurposeTo compare two angiography systems of different image capture technology, one with flat detector (FD) and one with image intensifier (II), in terms of entrance surface air kerma (ESAK) rate, detector dose (DD) rate and image quality (IQ), in interventional cardiology procedures concerning both adult and pediatric patients.Materials and methodsIn order to determine ESAK and DD rates, a digital dosimeter and polymethylmethacrylate (PMMA) plates were used. For the evaluation of IQ, two contrast objects (the Leeds TOR 18FG and a 5 mm-thick Aluminum plate) were used and two figures of merit were defined in fluoroscopy and cine acquisition modes. Measurements of ESAK, DD rates and IQ were made for various fields of view, pulse and frame acquisition rates.ResultsFor the particular setup used in this study was noted that ESAK values in the II system were generally larger than the respective values in the FD system (on average 70% for fluoro mode, 5 times for cine mode). When halving the fluoroscopy pulse rate, reduction in ESAK was not proportional, in fluoroscopy mode. Image quality evaluations indicated that II performs better in terms of low contrast sensitivity (LCS) and signal-to-noise ratio (SNR) than the FD system which performs better regarding high contrast resolution (HCR). However, when considering image quality in relation to ESAK the FD system performs better than the II system (with the exception of low thicknesses and zooms for high pulse rates in the fluoroscopy mode).ConclusionsThe FD system, generally, provides a better image quality–dose relation than the II system although II unit provides better LCS and SNR. This means that with the right adjustments to both systems, FD unit is able to provide same image quality with lower dose. However, newer technology does not automatically imply better image quality and further investigation is necessary for deriving safe conclusions for units which utilize different capture technology.     

Wild boar hunting and socioeconomic trends in Northern Greece, 1993–2002

Wild boar (Sus scrofa) populations increased worldwide in the last decade, but boar hunters have decreased. To maintain and increase hunters and to clarify the problems for sustainable hunting, we investigated their activities, attitudes, and socioeconomic trends. Drama and Kavala, Northern Greece have the best wild boar habitat in the country. We estimated the percentage of hunters hunting wild boar in Drama and Kavala and studied their profile to determine how the recent socioeconomic changes in the region affected numbers, activities, attitudes, and socioeconomic trend, from 1993 to 2002. In 1993, data were gathered through a questionnaire distributed randomly to 411 and 480 hunters in Drama and Kavala, respectively. In 2002, 320 and 400 questionnaires were collected in Drama and Kavala, respectively. In Drama, 13.6% of the hunters hunted wild boar in 1993 and 12.3% in 2002. In Kavala, 10.0% hunted wild boar in 1993 and 9.3% in 2002. The average age of hunters in Drama in 1993 was 37.9 years and 39.4 in 2002. In Kavala, the average age per hunter was reduced from 42.3 to 37.4 years. The average hunting experience had increased by 4.8% and 0.6%, respectively. In 2002, there was an increase in the distance traveled for hunting, the level of education of the hunters, the number of people working in the private sector, and in the public service. Wild boar hunters are mainly married, middle income class, earning 4,000–7,500 Euros/year. The above data will allow managers to better understand the socioeconomics of the hunters of wild boar and may be able to use the data to encourage increased hunting.