The antigenic activity of vaccines made from opportunistic microorganisms in an experiment to immunize animals by oral and combined methods]

The results of the study of the antigenic activity of multicomponent vaccine consisting of staphylococcal, Klebsiella, Proteus, Escherichia coli antigens and staphylococcal monovaccine, introduced into rabbits and guinea pigs by different routes, are presented. As shown in this study, the multicomponent vaccine introduced orally in 5 administrations stimulated the production of antibodies to all components, but the intensity of antibody formation to each of them was different. Antibodies to E. coli antigen were found to be at the lowest level. Staphylococcal antigen, introduced orally both as the component of the multicomponent vaccine and as the monovaccine, ensured pronounced stimulation of the production of antistaphylococcal antibodies, not lower than that observed after subcutaneous injection. The comparative study of the activity of the multicomponent vaccine introduced by combined routes, oral-subcutaneous and subcutaneous-oral, showed the advantage of the former one.     

Spatial structure of acid properties of litter in the succession row of swamp birch woods

The general potential, exchange, and actual (pH) acidities were investigated in the litter of the succession row of swamp birch woods. Their variabilities constitute, respectively, 75.9–174.4, 3.7–25.8 mmol (+)/100 g of the sampling, 3.7–5.5. For the first time, using the methods of geostatistics, their spatial variability was analyzed and the contributions of the trend, autocorrelation component, and the radius of the spatial correlation were estimated. It was established that in combination with the woody plants detritus, which is uniformly distributed along the ecological profile, the specific composition of the grass-moss tier, which corresponds to the humidity of edaphon, forms the picture of the spatial structure of acid properties of the litter. It was noted that the prime cause of variability consists in the particularities of the water regime of the habitats of swamp birch woods.     

Postvaccinal reactions to the administration of a dried staphylococcal vaccine made from water-soluble antigens

An unequivocal regularity in local and systemic reactions to multiple (in 5 injections) administration of lyophilized staphylococcal vaccine prepared from water-soluble antigens has been established in patients and in healthy adults: the number and intensity of reaction decreased after each subsequent injection. A similar tendency has been observed in the vaccinees who were examined for their IgE levels. These data are indicative of the desensitizing action of the vaccine.     

Allele specificity of genetic recombination in T4 phage using the indicator crossing study method]

The diagrams of relative correction ability of eighteen rII mutants of T4 phage were constructed on the basis of two-factor crosses, which were grouped into indicator series. In each series a pair of closely linked compared markers was crossed against indicator ones, the latter being distant enough so as to avoid simultaneous correction with the compared marker. The differences between the frequencies of wild type recombinants in crosses of two compared markers with indicator ones remained constant within the series and can be used as a measure of the differences between the compared markers in their correction ability. Mutants of base substitution type have small but statistically significant differences in correction ability. Simultaneous substitution of two bases in one codon yields a mutant which shows higher correction ability when compared to the mutant obtained as a result of substitution of only one base in the same codon. Frame shift mutants show much wider range of correctibility: some of them are corrected more rarely and others more frequently than base substitution mutants are.     

Immunostimulating activity of combined antigens of Klebsiella pneumoniae, Staphylococcus, Proteus and Escherichia coli and separate components in cross infection

Experiments on mice have revealed that the combined preparation of K. pneumoniae, Staphylococcus, Proteus and E. coli antigens possesses pronounced immunostimulating activity against the microorganisms whose antigens make up this combined preparation, as well as against Haemophilus influenzae, type b. Cross protection between the antigens making up the preparation was observed, as well as of each of its components with respect to H. influenzae, type b. The activity of the combined antigenic preparation has been shown to exceed that of its monocomponents, as manifested under the conditions of cross infection. These experimental data may serve as a prerequisite for the development of an immunostimulant for the treatment of patients with chronic pyoinflammatory diseases.     

Experimental study of the immunogenicity of a monopreparation of Klebsiella pneumoniae antigen complex and its association with Staphylococcus, Proteus and Escherichia coli antigens

The study carried out in mice with experimental Klebsiella sepsis has revealed that Staphylococcus, Proteus and E. coli antigenic complexes used as monovaccines ensure the protection of a definite percentage of the animals from K. pneumoniae infection. The mixture of these 3 preparations possesses a higher protective potency. The immunogenic potency of K. pneumoniae antigenic complex used as a component of combined vaccines with 2 or 4 components has proved to be sufficiently high and not inferior to the potency of K. pneumoniae monovaccine.