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排序方式: 共有238条查询结果,搜索用时 531 毫秒
61.
Interaction properties of the procollagen C-proteinase enhancer protein shed light on the mechanism of stimulation of BMP-1 总被引:4,自引:0,他引:4
Ricard-Blum S Bernocco S Font B Moali C Eichenberger D Farjanel J Burchardt ER van der Rest M Kessler E Hulmes DJ 《The Journal of biological chemistry》2002,277(37):33864-33869
Procollagen C-proteinase enhancer (PCPE) is an extracellular matrix glycoprotein that binds to the C-propeptide of procollagen I and can enhance the activities of procollagen C-proteinases up to 20-fold. To determine the molecular mechanism of PCPE activity, the interactions of the recombinant protein with the procollagen molecule as well as with its isolated C-propeptide domain were studied using surface plasmon resonance (BIAcore) technology. Binding required the presence of divalent metal cations such as calcium and manganese. By ligand blotting, calcium was found to bind to the C-propeptide domains of procollagens I and III but not to PCPE. By chemical cross-linking, the stoichiometry of the PCPE/C-propeptide interaction was found to be 1:1 in accordance with enzyme kinetic data. The use of a monoclonal antibody directed against the N-terminal region of the C-propeptide suggested that this region is probably not involved in binding to PCPE. Association and dissociation kinetics of the C-propeptide domains of procollagens I and III on immobilized PCPE were rapid. Extrapolation to saturation equilibrium yielded apparent equilibrium dissociation constants in the range 150-400 nM. In contrast, the association/dissociation kinetics of intact procollagen molecules on immobilized PCPE were relatively slow, corresponding to a dissociation constant of 1 nM. Finally, pN-collagen (i.e. procollagen devoid of the C-terminal propeptide domain) was also found to bind to immobilized PCPE, suggesting that PCPE binds to sites on either side of the procollagen cleavage site, thereby facilitating the action of procollagen C-proteinases. 相似文献
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Shifman S Bronstein M Sternfeld M Pisanté-Shalom A Lev-Lehman E Weizman A Reznik I Spivak B Grisaru N Karp L Schiffer R Kotler M Strous RD Swartz-Vanetik M Knobler HY Shinar E Beckmann JS Yakir B Risch N Zak NB Darvasi A 《American journal of human genetics》2002,71(6):1296-1302
Several lines of evidence have placed the catechol-O-methyltransferase (COMT) gene in the limelight as a candidate gene for schizophrenia. One of these is its biochemical function in metabolism of catecholamine neurotransmitters; another is the microdeletion, on chromosome 22q11, that includes the COMT gene and causes velocardiofacial syndrome, a syndrome associated with a high rate of psychosis, particularly schizophrenia. The interest in the COMT gene as a candidate risk factor for schizophrenia has led to numerous linkage and association analyses. These, however, have failed to produce any conclusive result. Here we report an efficient approach to gene discovery. The approach consists of (i) a large sample size-to our knowledge, the present study is the largest case-control study performed to date in schizophrenia; (ii) the use of Ashkenazi Jews, a well defined homogeneous population; and (iii) a stepwise procedure in which several single nucleotide polymorphisms (SNPs) are scanned in DNA pools, followed by individual genotyping and haplotype analysis of the relevant SNPs. We found a highly significant association between schizophrenia and a COMT haplotype (P=9.5x10-8). The approach presented can be widely implemented for the genetic dissection of other common diseases. 相似文献
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Miozes-Koch R Slabezki Y Efrat H Kalev H Kamer Y Yakobson Dag A 《Experimental & applied acarology》2000,24(1):35-43
The aim of this study was to explore the extent of varroa mite resistance to fluvalinate in Israel and to determine the underlying biochemical mechanism. Assays at different apiaries indicated varroa mite resistance at three of the five sites tested. Dose response assays conducted with tau-fluvalinate on mites obtained from different sites indicated uneven resistance. A monooxygenase assay revealed an increased rate (approximately 20-fold) of activity in mites that were not controlled by the pesticide, as compared to activity in mites from untreated colonies. A minor, 1.5–2.5 fold, increase of esterase activity was also noted in the resistant mites. This first demonstration of a fluvalinate-resistance mechanism in varroa mites points to the need for more vigorous resistance management practices to control the pest. 相似文献
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Sadagurski M Weingarten G Rhodes CJ White MF Wertheimer E 《The Journal of biological chemistry》2005,280(15):14536-14544
The insulin receptor substrate 2 (IRS-2) protein is one of the major insulin-signaling substrates. In the present study, we investigated the role of IRS-2 in skin epidermal keratinocytes and dermal fibroblasts. Although skin is not a classical insulin target tissue, we have previously demonstrated that insulin, via the insulin receptor, is essential for normal skin cell physiology. To identify the role of IRS-2 in skin cells, we studied cells isolated from IRS-2 knock-out (KO) mice. Whereas proliferation and differentiation were not affected in the IRS-2 KO cells, a striking effect was observed on glucose transport. In IRS-2 KO keratinocytes, the lack of IRS-2 resulted in a dramatic increase in basal and insulin-stimulated glucose transport. The increase in glucose transport was associated with an increase in total phosphatidylinositol (PI) 3-kinase and Akt activation. In contrast, fibroblasts lacking IRS-2 exhibited a significant decrease in basal and insulin-induced glucose transport. We identified the point of divergence, leading to these differences between keratinocytes and fibroblasts, at the IRS-PI 3-kinase association step. In epidermal keratinocytes, PI 3-kinase is associated with and activated by only the IRS-1 protein. On the other hand, in dermal fibroblasts, PI 3-kinase is exclusively associated with and activated by the IRS-2 protein. These observations suggest that IRS-2 functions as a negative or positive regulator of glucose transport in a cell-specific manner. Our results also show that IRS-2 function depends on its cell-specific association with PI 3-kinase. 相似文献
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Daphna Uni Elli Groner Elaine Soloway Amgad Hjazin Spencer Johnswick Gidon Winters Efrat Sheffer Ido Rog Yael Wagner Tamir Klein 《Journal of Plant Ecology》2021,14(1):117-131
生长在超干旱环境下的3种相思树种表现出异常低的叶片、树枝、树干、根中δ13C含量
在植物生理生态学中,叶片中碳13(13C)含量负值较少(富集),表明叶片处于通过气孔的气体交换减少,比如在干旱胁迫下。此外,与叶片相比,13C在非光合组织中的负值也较少。然而,对从叶片(光合器官)到树枝、树干和根(非光合器官)中的δ 13C数值的关系知之甚少,特别是缺少在关联密切的多个树种间或者不同器官间,以及对生长在极端高温和干旱胁迫下的树木中进行测定。本研究测定了3种近缘沙漠相思树种(Acacia tortilis、A. raddiana和A. pachyceras)从叶片到根的13C含量。我们在以色列南部成树的叶片组织中测定了δ 13C含量。与此同时,在试验果园进行了为期7年的3个水平的灌溉试验。在试验结束时,测定了叶片、树枝、树干和根的生长参数和δ 13C含量。研究结果表明,叶片组织中δ 13C含量约为−27‰,其同位素贫化程度远超过生长在地球上最干燥和最热环境中的沙漠树种的预期值。在不同的相思树种和不同器官中,所有灌溉水平处理中的δ 13C含量并没有富集(−28‰到ca. −27‰),证实了在成熟相思树中的测定结果。在不同器官中,叶片δ 13C含量与树枝和根的δ 13C含量异常相似,甚至比树干的δ 13C含量负值更少。高度贫化的叶片δ 13C表明,尽管这些树木生长在极端干燥的生境中,但其气孔气体交换较高。非光合组织中缺乏δ 13C富集可能与叶片和异养组织生长的季节耦合有关。 相似文献
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