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The composition of cell membranes and mycelia ofStreptomyces levoris, producer of the polyene antibiotic levorin, was studied. The membrane protein/lipid ratio was shown to be constant during
growth of the microorganism. The membrane protein was found to be heterogeneous and to have a low molecular weight. The lipid
component of the membranes consisted mainly of polar lipids—phosphatidyl ethanolamine, diphosphatidyl glycerol, and phosphatidyl
inositol mannoside being predominant. During growth, the phospholipid content of the polar lipid fraction decreased, apparently
due to replacement of phospholipids by phosphorus-free analogs. 相似文献
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Efimova A. P. Poliakova T. A. Belokon M. M. Belokon Y. S. Politov D. V. 《Russian Journal of Genetics》2019,55(5):551-556
Russian Journal of Genetics - The results of morphological and molecular genetic analysis confirming the hybrid nature of Salix × zhataica Efimova, Shurduk et Ahti are presented. In... 相似文献
218.
Changes in dipole potential of lipid bilayers ?d mimicking cell membranes induced by the adsorption of low-molecular-weight amphiphiles, flavonoids (phloretin and quercetin), and styrylpyridinium dyes (RH 421 and RH 237) were measured. A method based on the determination of ionophore-induced transmembrane current was used to evaluate changes in ?d after modifier addition. The characteristic parameters of the Langmuir adsorption isotherm and the greatest changes in ?d at an infinitely large concentration of flavonoid and its desorption constant, which reflects the affinity of the flavonoid to the lipid phase, were determined. The slopes of linear dependences of ?d increasing on the concentration of the styrylpiridinium dyes in membrane-bathing solution were defined. It was found that the dipole-modifying effect of phloretin depends on the charge of the lipids forming the membranes, while the ability of quercetin to reduce ?d is determined by the initial hydration of the bilayer. The results indicate that there are different mechanisms of the decrease in ?d upon the adsorption of the tested flavonoids. It was shown that the changes in ?d at the incorporation of styrylpyridinium dyes into bilayers are determined by the interaction of modifiers with membrane components. 相似文献
219.
Karyological analysis was made of G-banded chromosomes in the cells of three independent CHO-K1 clones stably resistant to colchicine (Clch) selected for resistance to Clch at the concentration 0.1 mkg/ml (the first step of selection), and of one clone with higher but unstable level of resistance (2 mkg/ml--the second level of resistance). The results obtained revealed a morphological instability of the P-shoulder of chromosome Z6: more often an additional genetical material (AGM) at the distal end (Z6+), or rarely deletions (Z6-). In cells of the stable resistant clones of the first step of selection the length of AGM and their morphological structures were shown to be constant, but differed among the clones. In cells of the higher resistant unstable clone the length of AGM and their morphological structure were different in different cells within the clone. The AGMs in the Clch-resistant cells are discussed in terms of possible amplification of gene(s) responsible for the Clch resistance. In the cell population of clone selected for the resistance to 2 mkg/ml of Clch the frequency of rearranged chromosomes was shown to increase. In cells of all the analysed resistant clones the chromosome Z16 was found to lose its p-shoulder. 相似文献
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The Priming of Potato Plants Induced by Brassinosteroids Reduces Oxidative Stress and Increases Salt Tolerance 总被引:1,自引:0,他引:1
M. V. Efimova V. A. Khripach E. V. Boyko M. K. Malofii L. V. Kolomeichuk O. K. Murgan A. N. Vidershpun E. A. Mukhamatdinova V. V. Kuznetsov 《Doklady biological sciences》2018,478(1):33-36
This is the first study to show that brief pretreatment of potato plants with two brassinosteroids differing in structure causes in plants the ability to react to delayed salt stress by accumulation of compounds with antioxidant activity and by increased salt tolerance. 相似文献