Nutritional status and endocrine response to hemorrhage

Hyperglycemia-inducing hyperosmolality has recently been proven beneficial in the maintenance of blood volume and extracellular fluid volume during early hemorrhagic hypotension. Fed animals benefitted from better plasma refill compared with starved ones when subjected to equal blood loss. Using lightly sedated fed and 24-30 h starved rats, hormones with relevance to glucose homeostasis were studied during 90 min of hemorrhagic hypotension of 70 mmHg (1 mmHg = 133.32 Pa). Marked differences in the overall hormonal developments were found between the two groups. In fed rats, insulin and glucagon responses were initially attenuated, while somatostatin increased to an early peak level at 30 min, returning to basal at 90 min. In starved rats, somatostatin increased gradually during the 90 min. Adrenaline release was massive in both groups. Corticosterone showed no increase from basal levels in the fed group during hemorrhage, while starved rats increased their basal level fourfold already at 30 min. These data are presented as evidence that changing nutritional status alters hormonal response to hypovolemic stress.     

Phospholipids in myofibrils

The myofibrils were shown to be lipoprotein structures. Lipoprotein nature of these structures was confirmed by their lipid phosphorus to protein ratio in a completely dispersed myofibril preparation of the skeletal muscle cells. The ratio was found to be 49.65 ± 3.95 microgram P / mg protein. The phospholipids present in the myofibril preparation were determined to be phosphatidylcholine and phosphatidylethanolamine.