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101.
Posada JM Sievänen R Messier C Perttunen J Nikinmaa E Lechowicz MJ 《Annals of botany》2012,110(3):731-741
Background and Aims
Plants are expected to maximize their net photosynthetic gains and efficiently use available resources, but the fundamental principles governing trade-offs in suites of traits related to resource-use optimization remain uncertain. This study investigated whether Acer saccharum (sugar maple) saplings could maximize their net photosynthetic gains through a combination of crown structure and foliar characteristics that let all leaves maximize their photosynthetic light-use efficiency (ɛ).Methods
A functional–structural model, LIGNUM, was used to simulate individuals of different leaf area index (LAIind) together with a genetic algorithm to find distributions of leaf angle (LA) and leaf photosynthetic capacity (Amax) that maximized net carbon gain at the whole-plant level. Saplings grown in either the open or in a forest gap were simulated with Amax either unconstrained or constrained to an upper value consistent with reported values for Amax in A. saccharum.Key Results
It was found that total net photosynthetic gain was highest when whole-plant PPFD absorption and leaf ɛ were simultaneously maximized. Maximization of ɛ required simultaneous adjustments in LA and Amax along gradients of PPFD in the plants. When Amax was constrained to a maximum, plants growing in the open maximized their PPFD absorption but not ɛ because PPFD incident on leaves was higher than the PPFD at which ɛmax was attainable. Average leaf ɛ in constrained plants nonetheless improved with increasing LAIind because of an increase in self-shading.Conclusions
It is concluded that there are selective pressures for plants to simultaneously maximize both PPFD absorption at the scale of the whole individual and ɛ at the scale of leaves, which requires a highly integrated response between LA, Amax and LAIind. The results also suggest that to maximize ɛ plants have evolved mechanisms that co-ordinate the LA and Amax of individual leaves with PPFD availability. 相似文献102.
Odile Thiéry Mari Moora Martti Vasar Martin Zobel Maarja Öpik 《Symbiosis (Philadelphia, Pa.)》2012,58(1-3):135-147
Most molecular ecological studies of arbuscular mycorrhizal fungi (AMF) have been based on the rRNA gene sequences. However, information about intraspecific nucleotide variation is still limited in these fungi. In this study, we calculated the inter- and intrasporal nucleotide variation of Diversispora sp. EE1 using 78 cloned sequences from four spores within a ca 4960 bp fragment of the nuclear ribosomal operon spanning the near full length small ribosomal subunit (SSU) rRNA gene, the full internal transcribed spacer (ITS: ITS1-5.8S-ITS2) and ca 2740 bp of the large ribosomal subunit (LSU) rRNA gene. Data for each marker region (SSU, ITS and LSU) originated from the very same spores. Sequence variation resulting from point mutations and small indels was recorded in all regions. Highest sequence variation was observed in the ITS region at both the inter- and intrasporal levels. The ITS1 component was more variable than ITS2, whilst the 5.8S gene was the least variable component of the ITS region. Evolutionary divergence of gene copies between spores was intermediate for the LSU and lowest for the SSU. The SSU and the LSU genes had relatively similar evolutionary divergence per spore. Sequence variant richness was not exhaustive for any of the marker regions, indicating that multiple sequences per spore from multiple spores are needed when characterizing a species. This study provides reference sequences for ecological studies, permitting identification of AMF using any of the ribosomal regions or primer systems. 相似文献
103.
CD2-associated protein is widely expressed and differentially regulated during embryonic development 总被引:1,自引:0,他引:1
Lehtonen S Tienari J Londesborough A Pirvola U Ora A Reima I Lehtonen E 《Differentiation; research in biological diversity》2008,76(5):506-517
CD2-associated protein (CD2AP) is an adapter protein that is involved in various signaling and vesicular trafficking processes and also functions as a linker between plasma membrane proteins and the actin cytoskeleton. The protein is known to have important functions in T cells and glomerular podocytes, but it is also expressed by many other adult-type tissues and cells. Here we analyzed the expression of the protein during early embryonic development and organogenesis of the mouse. The results showed differential tissue-specific regulation of CD2AP in developing and maturing organs. In oocytes and pre-implantation embryos, CD2AP was located diffusely in the cytoplasm, whereas in late blastocysts it was concentrated to the intercellular contacts. During organogenesis, CD2AP was distinctly upregulated upon, e.g., the pretubular aggregation of metanephric mesenchyme cells and the appearance of the osteoblastic rim around cartilages during endochondral ossification. High CD2AP expression was also observed during epithelial-like conversion of some highly specialized secretory cell types such as the odontoblasts, the cells of the choroid plexus and the decidualized cells of the endometrial stroma. In other instances, such as the development of the proximal tubuli of the kidney and the flat alveolar epithelium of the lung, the protein was downregulated upon differentiation and maturation of the cells. Finally, certain cells, e.g., glomerular podocytes, those forming the collecting ducts of the kidney, and the urothelium of the kidney pelvis, expressed CD2AP throughout their differentiation and maturation. Multiple molecules and complex pathways regulate embryogenesis, and scaffolding proteins apparently have pivotal roles in targeting and finetuning, e.g., growth factor- or hormone-induced processes. The cell-type specific spatio-temporal regulation of CD2AP during development suggests that this adapter protein is a key regulatory partner in many signaling pathways and cellular processes governing differentiation and morphogenesis. 相似文献
104.
Linking xylem diameter variations with sap flow measurements 总被引:1,自引:0,他引:1
Sanna Sevanto Eero Nikinmaa Anu Riikonen Michael Daley J. Cory Pettijohn Teis N. Mikkelsen Nathan Phillips N. Michele Holbrook 《Plant and Soil》2008,305(1-2):77-90
Measurements of variation in the diameter of tree stems provide a rapid response, high resolution tool for detecting changes
in water tension inside the xylem. Water movement inside the xylem is caused by changes in the water tension and theoretically,
the sap flow rate should be directly proportional to the water tension gradient and, therefore, also linearly linked to the
xylem diameter variations. The coefficient of proportionality describes the water conductivity and elasticity of the conducting
tissue. Xylem diameter variation measurements could thus provide an alternative approach for estimating sap flow rates, but
currently we lack means for calibration. On the other hand, xylem diameter variation measurements could also be used as a
tool for studying xylem structure and function. If we knew both the water tension in the xylem and the sap flow rate, xylem
conductivity and/or elasticity could be calculated from the slope of their relationship. In this study we measured diurnal
xylem diameter variation simultaneously with sap flow rates (Granier-type thermal method) in six deciduous species (Acer rubrum L., Alnus glutinosa Miller, Betula lenta L., Fagus Sylvatica L. Quercus rubra L., and Tilia vulgaris L.) for 7–91 day periods during summers 2003, 2005 and 2006 and analyzed the relationship between these two measurements.
We found that in all species xylem diameter variations and sap flow rate were linearly related in daily scale (daily average
R
2 = 0.61–0.87) but there was a significant variation in the daily slopes of the linear regressions. The largest variance in
the slopes, however, was found between species, which is encouraging for finding a species specific calibration method for
measuring sap flow rates using xylem diameter variations. At a daily timescale, xylem diameter variation and sap flow rate
were related to each other via a hysteresis loop. The slopes during the morning and afternoon did not differ statistically
significantly from each other, indicating no overall change in the conductivity. Because of the variance in the daily slopes,
we tested three different data averaging methods to obtain calibration coefficients. The performance of the averaging methods
depended on the source of variance in the data set and none of them performed best for all species. The best estimates of
instantaneous sap flow rates were also given by different averaging methods than the best estimates of total daily water use.
Using the linear relationship of sap flow rate and xylem diameter variations we calculated the conductance and specific conductivity
of the soil–xylem–atmosphere water pathway. The conductance were of the order of magnitude 10−5 kg s−1 MPa−1 for all species, which compares well with measured water fluxes from broadleaved forests. Interestingly, because of the large
sap wood area the conductance of Betula was approximately 10 times larger than in other species. 相似文献
105.
106.
Plant traits determine the phylogenetic structure of arbuscular mycorrhizal fungal communities 总被引:1,自引:0,他引:1
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Álvaro López‐García Sara Varela‐Cervero Martti Vasar Maarja Öpik José M. Barea Concepción Azcón‐Aguilar 《Molecular ecology》2017,26(24):6948-6959
Functional diversity in ecosystems has traditionally been studied using aboveground plant traits. Despite the known effect of plant traits on the microbial community composition, their effects on the microbial functional diversity are only starting to be assessed. In this study, the phylogenetic structure of arbuscular mycorrhizal (AM) fungal communities associated with plant species differing in life cycle and growth form, that is, plant life forms, was determined to unravel the effect of plant traits on the functional diversity of this fungal group. The results of the 454 pyrosequencing showed that the AM fungal community composition differed across plant life forms and this effect was dependent on the soil collection date. Plants with ruderal characteristics tended to associate with phylogenetically clustered AM fungal communities. By contrast, plants with resource‐conservative traits associated with phylogenetically overdispersed AM fungal communities. Additionally, the soil collected in different seasons yielded AM fungal communities with different phylogenetic dispersion. In summary, we found that the phylogenetic structure, and hence the functional diversity, of AM fungal communities is dependent on plant traits. This finding adds value to the use of plant traits for the evaluation of belowground ecosystem diversity, functions and processes. 相似文献
107.
Multiple Dimensions of Socioeconomic Position and Obesity among Employees: The Helsinki Health Study
Mikko Laaksonen Sirpa Sarlio‐Lhteenkorva Eero Lahelma 《Obesity (Silver Spring, Md.)》2004,12(11):1851-1858
Objective: To examine socioeconomic differences in obesity using several different socioeconomic indicators, ranging from childhood socioeconomic environment and adult socioeconomic status to material resources and economic satisfaction. Research Methods and Procedures: The data derived from the Helsinki Health Study baseline surveys in 2000 and 2001. Respondents to postal surveys were middle‐aged employees of the City of Helsinki (4, 975 women and 1, 252 men, response rate 68%). Associations between eight socioeconomic indicators and obesity (BMI ≥ 30 kg/m2), calculated from self‐reported data, were examined by fitting a series of logistic regression models. Results: In women, all socioeconomic indicators except household income and economic satisfaction were associated with obesity. Parental education and childhood economic difficulties, i.e., socioeconomic conditions in childhood, remained associated with obesity after adjusting for all indicators of current socioeconomic position. Indicators of adult socioeconomic status, own education and occupational class, were no longer associated with obesity when childhood socioeconomic conditions were adjusted for. Home ownership and economic difficulties were associated with obesity after full adjustments. In men, the findings paralleled those among women, but few associations reached statistical significance. Discussion: Obesity was associated with several dimensions of socioeconomic position. Childhood socioeconomic disadvantage was associated with obesity independently of the various indicators of current socioeconomic position. Associations between obesity and both educational level and occupational class disappeared after adjustment for other indicators of socioeconomic position. This suggests that the variation observed in the prevalence of obesity by these key socioeconomic indicators may reflect differences in the related material resources. 相似文献
108.
Air temperature triggers the recovery of evergreen boreal forest photosynthesis in spring 总被引:11,自引:0,他引:11
Suni Tanja Frank Berninger† Timo Vesala Tiina Markkanen Pertti Hari† Annikki Mäkelä† Hannu Ilvesniemi† Heikki Hänninen‡ Eero Nikinmaa† Timo Huttula Tuomas Laurila§ Mika Aurela§ Achim Grelle¶ ers Lindroth Almut Arneth Olga Shibistova†† Jon Lloyd 《Global Change Biology》2003,9(10):1410-1426
The timing of the commencement of photosynthesis (P*) in spring is an important determinant of growing‐season length and thus of the productivity of boreal forests. Although controlled experiments have shed light on environmental mechanisms triggering release from photoinhibition after winter, quantitative research for trees growing naturally in the field is scarce. In this study, we investigated the environmental cues initiating the spring recovery of boreal coniferous forest ecosystems under field conditions. We used meteorological data and above‐canopy eddy covariance measurements of the net ecosystem CO2 exchange (NEE) from five field stations located in northern and southern Finland, northern and southern Sweden, and central Siberia. The within‐ and intersite variability for P* was large, 30–60 days. Of the different climate variables examined, air temperature emerged as the best predictor for P* in spring. We also found that ‘soil thaw’, defined as the time when near‐surface soil temperature rapidly increases above 0°C, is not a useful criterion for P*. In one case, photosynthesis commenced 1.5 months before soil temperatures increased significantly above 0°C. At most sites, we were able to determine a threshold for air‐temperature‐related variables, the exceeding of which was required for P*. A 5‐day running‐average temperature (T5) produced the best predictions, but a developmental‐stage model (S) utilizing a modified temperature sum concept also worked well. But for both T5 and S, the threshold values varied from site to site, perhaps reflecting genetic differences among the stands or climate‐induced differences in the physiological state of trees in late winter/early spring. Only at the warmest site, in southern Sweden, could we obtain no threshold values for T5 or S that could predict P* reliably. This suggests that although air temperature appears to be a good predictor for P* at high latitudes, there may be no unifying ecophysiological relationship applicable across the entire boreal zone. 相似文献
109.
English JL Kassiri Z Koskivirta I Atkinson SJ Di Grappa M Soloway PD Nagase H Vuorio E Murphy G Khokha R 《The Journal of biological chemistry》2006,281(15):10337-10346
Membrane-type matrix metalloproteinases (MT-MMPs) have emerged as key enzymes in tumor cell biology. The importance of MT1-MMP, in particular, is highlighted by its ability to activate pro-MMP-2 at the cell surface through the formation of a trimolecular complex comprised of MT1-MMP/tissue inhibitor of metalloproteinase-2 (TIMP-2)/pro-MMP-2. TIMPs 1-4 are physiological MMP inhibitors with distinct roles in the regulation of pro-MMP-2 processing. Here, we have shown that individual Timp deficiencies differentially affect MMP-2 processing using primary mouse embryonic fibroblasts (MEFs). Timp-3 deficiency accelerated pro-MMP-2 activation in response to both cytochalasin D and concanavalin A. Exogenous TIMP-2 and N-TIMP-3 inhibited this activation, whereas TIMP-3 containing matrix from wild-type MEFs did not rescue the enhanced MMP-2 activation in Timp-3(-/-) cells. Increased processing of MMP-2 did not arise from increased expression of MT1-MMP, MT2-MMP, or MT3-MMP or altered expression of TIMP-2 and MMP-2. To test whether increased MMP-2 processing in Timp-3(-/-) MEFs is dependent on TIMP-2, double deficient Timp-2(-/-)/-3(-/-) MEFs were used. In these double deficient cells, the cleavage of pro-MMP-2 to its intermediate form was substantially increased, but the subsequent cleavage of intermediate-MMP-2 to fully active form, although absent in Timp-2(-/-) MEFs, was detectable with combined Timp-2(-/-)/-3(-/-) deficiency. TIMP-4 associates with MMP-2 and MT1-MMP in a manner similar to TIMP-3, but its deletion had no effect on pro-MMP-2 processing. Thus, TIMP-3 provides an inherent regulation over the kinetics of pro-MMP-2 processing, serving at a level distinct from that of TIMP-2 and TIMP-4. 相似文献
110.
Synopsis A comparison was done between growth in Atlantic salmon,Salmo salar, parr from natal rivers and small tributaries in the subarctic River Teno watercourse in northern Finland (70° N, 28° E).
Salmon do not spawn in these tributaries but juveniles enter the brooks from their spawning rivers. Parr from these brooks
(age groups 1 + and 2 +) were larger, so were their recorded annual growth increments, than those caught simultaneously in
the River Teno. First year growth was also better in brook parr, although both groups had spent their first year in the main
river. The brook water temperature was lower than that in the main rivers through-out the growing season. On the other hand,
drifting food resources were more abundant in brooks. It has been suggested that better growth and a longer residence in fresh
water result in the larger smolts being produced in the brooks relative to the main river. Differences in habitat selection
and subsequent differences in the smolt age and size of fish from the same place of origin reflect the distinct life histories
of the salmon in the River Teno. 相似文献