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排序方式: 共有250条查询结果,搜索用时 46 毫秒
241.
Andrew Travers Edwige Hiriart Mark Churcher Micaela Caserta Ernesto Di Mauro 《Journal of biomolecular structure & dynamics》2013,31(6):713-724
Abstract The contribution of histone-DNA interactions to nucleosome positioning in vivo is currently a matter of debate. We argue here that certain nucleosome positions, often in promoter regions, in yeast may be, at least in part, specified by the DNA sequence. In contrast other positions may be poorly specified. Positioning thus has both statistical and DNA-determined components. We further argue that the relative affinity of the octamer for different DNA sequences can vary and therefore the interaction of histones with the DNA is a ‘tunable’ property. 相似文献
242.
Sanela Vellino Christiane Oddou Paul Rivier Cyril Boyault Edwige Hiriart-Bryant Alexandra Kraut Ren Martin Yohann Coute Hans-Joachim Knlker Miguel A. Valverde Corinne Albigs-Rizo Olivier Destaing 《The Journal of cell biology》2021,220(2)
Invadosomes support cell invasion by coupling both acto-adhesive and extracellular matrix degradative functions, which are apparently antagonistic. β1-integrin dynamics regulate this coupling, but the actual sensing mechanism and effectors involved have not yet been elucidated. Using genetic and reverse genetic approaches combined with biochemical and imaging techniques, we now show that the calcium channel TRPV4 colocalizes with β1-integrins at the invadosome periphery and regulates its activation and the coupling of acto-adhesive and degradative functions. TRPV4-mediated regulation of podosome function depends on its ability to sense reactive oxygen species (ROS) in invadosomes’ microenvironment and involves activation of the ROS/calcium-sensitive kinase Ask1 and binding of the motor MYO1C. Furthermore, disease-associated TRPV4 gain-of-function mutations that modulate ECM degradation are also implicated in the ROS response, which provides new perspectives in our understanding of the pathophysiology of TRPV4 channelopathies. 相似文献
243.
Gautreau Edwige Volatier Laurence Nogaro Géraldine Gouze Emma Mermillod-Blondin Florian 《Hydrobiologia》2020,847(4):1027-1040
Hydrobiologia - Sediments are sinks of nutrients in reservoirs, but may also act as temporary nutrient sources to the water column, leading to eutrophication during the warm season. Several abiotic... 相似文献
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Moïra B Dion Pier-Luc Plante Edwige Zufferey Shiraz A Shah Jacques Corbeil Sylvain Moineau 《Nucleic acids research》2021,49(6):3127
Thousands of new phages have recently been discovered thanks to viral metagenomics. These phages are extremely diverse and their genome sequences often do not resemble any known phages. To appreciate their ecological impact, it is important to determine their bacterial hosts. CRISPR spacers can be used to predict hosts of unknown phages, as spacers represent biological records of past phage–bacteria interactions. However, no guidelines have been established to standardize host prediction based on CRISPR spacers. Additionally, there are no tools that use spacers to perform host predictions on large viral datasets. Here, we developed a set of tools that includes all the necessary steps for predicting the hosts of uncharacterized phages. We created a database of >11 million spacers and a program to execute host predictions on large viral datasets. Our host prediction approach uses biological criteria inspired by how CRISPR–Cas naturally work as adaptive immune systems, which make the results easy to interpret. We evaluated the performance using 9484 phages with known hosts and obtained a recall of 49% and a precision of 69%. We also found that this host prediction method yielded higher performance for phages that infect gut-associated bacteria, suggesting it is well suited for gut-virome characterization. 相似文献
246.
Anaïs Llorens Athina Tzovara Ludovic Bellier Ilina Bhaya-Grossman Aurélie Bidet-Caulet William K. Chang Zachariah R. Cross Rosa Dominguez-Faus Adeen Flinker Yvonne Fonken Mark A. Gorenstein Chris Holdgraf Colin W. Hoy Maria V. Ivanova Richard T. Jimenez Soyeon Jun Julia W.Y. Kam Celeste Kidd Nina F. Dronkers 《Neuron》2021,109(13):2047-2074
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Yariv Brotman Cindy Llorente-Wiegand Glenn Oyong Saurabh Badoni Gopal Misra Roslen Anacleto Sabiha Parween Erstelle Pasion Rhowell N. Tiozon Jr. Joanne J. Anonuevo Maria K. deGuzman Saleh Alseekh Edwige G. N. Mbanjo Lesley A. Boyd Alisdair R. Fernie Nese Sreenivasulu 《The Plant journal : for cell and molecular biology》2021,106(2):507-525
Brown rice (Oryza sativa) possesses various nutritionally dense bioactive phytochemicals exhibiting a wide range of antioxidant, anti-cancer, and anti-diabetic properties known to promote various human health benefits. However, despite the wide claims made about the importance of brown rice for human nutrition the underlying metabolic diversity has not been systematically explored. Non-targeted metabolite profiling of developing and mature seeds of a diverse genetic panel of 320 rice cultivars allowed quantification of 117 metabolites. The metabolite genome-wide association study (mGWAS) detected genetic variants influencing diverse metabolic targets in developing and mature seeds. We further interlinked genetic variants on chromosome 7 (6.06–6.43 Mb region) with complex epistatic genetic interactions impacting multi-dimensional nutritional targets, including complex carbohydrate starch quality, the glycemic index, antioxidant catechin, and rice grain color. Through this nutrigenomics approach rare gene bank accessions possessing genetic variants in bHLH and IPT5 genes were identified through haplotype enrichment. These variants were associated with a low glycemic index, higher catechin levels, elevated total flavonoid contents, and heightened antioxidant activity in the whole grain with elevated anti-cancer properties being confirmed in cancer cell lines. This multi-disciplinary nutrigenomics approach thus allowed us to discover the genetic basis of human health-conferring diversity in the metabolome of brown rice. 相似文献
249.
Hannelore Strosse Edwige Andre László Sági Rony Swennen Bart Panis 《Plant Cell, Tissue and Organ Culture》2008,95(3):321-332
Despite their similar morphology, banana and maize shoot tips responded strikingly different with respect to the in vitro
formation of homogeneous multiple shoot clusters. While up to 50 small shoots per maize explant could be induced within 1 month,
zero to one additional shoot formed starting from a banana shoot tip. Subsequently, banana shoot tips were subjected to different
combinations of five cytokinins (0–100 μM) and five auxins (0–5 μM). The cytokinins thidiazuron and benzylaminopurine stimulated
multiplication to a higher extent compared to zeatin, kinetin and isopentenyl adenine. The addition of indoleacetic acid,
naphthalene acetic acid or indolebutyric acid to cytokinin containing medium did not affect the in vitro response. In contrast,
2,4-dichlorophenoxyacetic acid (1 and 5 μM) and a higher concentration of picloram (5 μM) had a detrimental effect on shoot
formation and resulted in explant death and globule development. When small (0.1 cm) shoot tips were grown on cytokinin medium
without an auxin source, the average number of shoots was generally two to three times lower compared to bigger (0.5 cm) shoot
tips. Based on our experience in maize and this large-scale study with banana shoot tips, we conclude that banana is extremely
recalcitrant towards adventitious shoot formation. This recalcitrance could not be overcome by any of the 173 different plant
growth regulator combinations tested. In vitro multiplication of banana thus appears solely restricted to axillary shoot formation. 相似文献
250.