全文获取类型
收费全文 | 175篇 |
免费 | 14篇 |
出版年
2022年 | 3篇 |
2021年 | 2篇 |
2020年 | 2篇 |
2019年 | 5篇 |
2018年 | 1篇 |
2017年 | 2篇 |
2016年 | 4篇 |
2015年 | 11篇 |
2014年 | 13篇 |
2013年 | 5篇 |
2012年 | 13篇 |
2011年 | 11篇 |
2010年 | 7篇 |
2009年 | 5篇 |
2008年 | 4篇 |
2007年 | 11篇 |
2006年 | 14篇 |
2005年 | 6篇 |
2004年 | 7篇 |
2003年 | 5篇 |
2002年 | 9篇 |
2001年 | 4篇 |
2000年 | 5篇 |
1999年 | 4篇 |
1998年 | 1篇 |
1997年 | 4篇 |
1996年 | 2篇 |
1995年 | 5篇 |
1994年 | 2篇 |
1993年 | 2篇 |
1992年 | 4篇 |
1991年 | 1篇 |
1989年 | 2篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1984年 | 3篇 |
1983年 | 1篇 |
1979年 | 2篇 |
1977年 | 4篇 |
1973年 | 1篇 |
排序方式: 共有189条查询结果,搜索用时 15 毫秒
31.
Gornati Rosalba Basu Subhash Bernardini Giovanni Rizzo Angela M. Rossi Federica Berra Bruno 《Molecular and cellular biochemistry》1997,166(1-2):117-124
The activities of glycosyltransferases and sialidases, together with the ganglioside content and distribution, have been extensively studied in mammals, while the informations on tissues of other animals, including amphibian, are scarce. In this paper we present data on the activities of SAT-1, SAT-2, SAT-4, SAT-5, GlcNAcT-1, GalNAcT-1, GalT-6, and sialidases studied in Xenopus laevis embryos at different stages of development. The highest activity was found at days 4 and 5 of embryogenesis for glycosyltransferases and sialidases respectively; a tentative correlation between the in vitro activity of these enzymes and the content of neutral and acidic glycolipids is discussed. (Mol Cell Biochem 166: 117-124, 1997) 相似文献
32.
Angela M. Rizzo Gigliola Montorfano Manuela Negroni Paola Corsetto Patrizia Berselli Paola Marciani Stefania Zava Bruno Berra 《Cell biology international》2009,33(8):893-898
Space flights cause a number of patho-physiological changes. Oxidative damage has been demonstrated in astronauts after space flights. Oxidative stress is due to an imbalance between production of oxidant and antioxidative defence. In embryos of Xenopus laevis, the glutathione system is an inducible antioxidant defence. For this reason, we investigated the effect of gravity deprivation on endogenous antioxidant enzymes in X. laevis embryos developed for 6 days in a Random Positioning Machine. The results show that glutathione content and the activity of antioxidant enzymes increase in RPM embryos, suggesting the presence of a protective mechanism. An induction of antioxidant defence might play an important role for animals to adapt to micro-gravitational stress, possibly during actual space flights. 相似文献
33.
34.
Esther Conde Ana Suárez-Gauthier Amparo Benito Pilar Garrido Rosario García-Campelo Michele Biscuola Luis Paz-Ares David Hardisson Javier de Castro M. Carmen Camacho Delvys Rodriguez-Abreu Ihab Abdulkader Josep Ramirez Noemí Reguart Marta Salido Lara Pijuán Edurne Arriola Julián Sanz Victoria Folgueras Noemí Villanueva Javier Gómez-Román Manuel Hidalgo Fernando López-Ríos 《PloS one》2014,9(9)
Background
Based on the excellent results of the clinical trials with ALK-inhibitors, the importance of accurately identifying ALK positive lung cancer has never been greater. However, there are increasing number of recent publications addressing discordances between FISH and IHC. The controversy is further fuelled by the different regulatory approvals. This situation prompted us to investigate two ALK IHC antibodies (using a novel ultrasensitive detection-amplification kit) and an automated ALK FISH scanning system (FDA-cleared) in a series of non-small cell lung cancer tumor samples.Methods
Forty-seven ALK FISH-positive and 56 ALK FISH-negative NSCLC samples were studied. All specimens were screened for ALK expression by two IHC antibodies (clone 5A4 from Novocastra and clone D5F3 from Ventana) and for ALK rearrangement by FISH (Vysis ALK FISH break-apart kit), which was automatically captured and scored by using Bioview''s automated scanning system.Results
All positive cases with the IHC antibodies were FISH-positive. There was only one IHC-negative case with both antibodies which showed a FISH-positive result. The overall sensitivity and specificity of the IHC in comparison with FISH were 98% and 100%, respectively.Conclusions
The specificity of these ultrasensitive IHC assays may obviate the need for FISH confirmation in positive IHC cases. However, the likelihood of false negative IHC results strengthens the case for FISH testing, at least in some situations. 相似文献35.
Valentina Danesi Lorenzo Zani Axel Scheele Francesco Berra Luca Cristofolini 《Journal of biomechanics》2014
Definition of an anatomical reference frame is necessary for in vitro biomechanical testing. Nevertheless, there is neither a clear recommendation, nor consensus in the literature concerning an anatomical reference frame for in vitro testing of the human vertebrae. The scope of this work is to define a reference frame for the human vertebrae for in vitro applications. The proposed anatomical reference frame relies on alignment of well-defined points on the endplates, and on two landmarks on the posterior wall. The repeatability of the proposed alignment procedure has been tested in vitro by 5 operators, on 7 specimens. Furthermore, the feasibility and repeatability of the proposed procedure was assessed in silico, using CT-scans of the same specimens. 相似文献
36.
Samuel Gmez-Arcas Francisco Jos Muoz Adriana Ricarte-Bermejo ngela María Snchez-Lpez Marouane Baslam Edurne Baroja-Fernndez Abdellatif Bahaji Goizeder Almagro Nuria De Diego Karel Doleal Ondej Novk Jesús Leal-Lpez Rafael Jorge Len Morcillo Araceli G Castillo Javier Pozueta-Romero 《Plant physiology》2022,190(4):2137
37.
38.
Ophryotrocha labronica is a gonochoristic polychaete worm whose sex determining mechanism and sex ratio control are supposed to be polygenic. From a lab population, whose sex ratio (i.e., proportion of males) was 0.5, the estimate of sex ratio heritability by offspring-father regression was 0.54 ± 0.15 and by offspring-mother regression was not significantly different from 0. Estimate of sex ratio repeatability between successive broods of a pair was 0.64 ± 0.33. Since female parents do not contribute in any way to the variability of sex ratio, sex ratio variation seems to be largely a paternal character. On the basis of these estimates we advance the hypothesis that in this species sex is determined by a multilocus genetic system, allowing the combined effects of a female major sex gene (which could give rise to a form of female heterogamety) and masculinizing modifiers. The hypothesis that the male sex has the least canalised sexual differentiation is supported by the observation that some old males developed oocytes. 相似文献
39.
Modifications of Ganglioside Patterns in Human Meningiomas 总被引:1,自引:1,他引:0
B. Berra L. Riboni† R. De Gasperi S. M. Gaini‡ G. Ragnotti† 《Journal of neurochemistry》1983,40(3):777-782
Abstract: Ganglioside content and distribution were determined in control meninges and in 30 human meningiomas belonging to four different histological types. Irrespective of the histological classification all meningiomas showed a ganglioside content significantly higher than that of control meninges. The analysis of ganglioside distribution in each meningioma showed that in the majority of the cases the increase of ganglioside content was primarily the result of selective accumulation of ganglioside GM3 ; in the remaining cases ganglioside GM1 was present in a significantly higher amount than in the control dura mater and leptomeninges. A common feature of both types of meningiomas is a simplification of ganglioside pattern, with a shift from the polysialylated to the monosialylated forms. A tentative classification of meningiomas into "GM3 -rich" and "GM1 -rich" types, together with an explanation for the selective accumulation of these two types of ganglioside, is proposed. 相似文献
40.