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61.
Julie M. Grossman Brendan E. O’Neill Siu Mui Tsai Biqing Liang Eduardo Neves Johannes Lehmann Janice E. Thies 《Microbial ecology》2010,60(1):192-205
We compared the microbial community composition in soils from the Brazilian Amazon with two contrasting histories; anthrosols
and their adjacent non-anthrosol soils of the same mineralogy. The anthrosols, also known as the Amazonian Dark Earths or
terra preta, were managed by the indigenous pre-Colombian Indians between 500 and 8,700 years before present and are characterized
by unusually high cation exchange capacity, phosphorus (P), and calcium (Ca) contents, and soil carbon pools that contain
a high proportion of incompletely combusted biomass as biochar or black carbon (BC). We sampled paired anthrosol and unmodified
soils from four locations in the Manaus, Brazil, region that differed in their current land use and soil type. Community DNA
was extracted from sampled soils and characterized by use of denaturing gradient gel electrophoresis (DGGE) and terminal restriction
fragment length polymorphism. DNA bands of interest from Bacteria and Archaea DGGE gels were cloned and sequenced. In cluster
analyses of the DNA fingerprints, microbial communities from the anthrosols grouped together regardless of current land use
or soil type and were distinct from those in their respective, paired adjacent soils. For the Archaea, the anthrosol communities
diverged from the adjacent soils by over 90%. A greater overall richness was observed for Bacteria sequences as compared with
those of the Archaea. Most of the sequences obtained were novel and matched those in databases at less than 98% similarity.
Several sequences obtained only from the anthrosols grouped at 93% similarity with the Verrucomicrobia, a genus commonly found in rice paddies in the tropics. Sequences closely related to Proteobacteria and Cyanobacteria sp. were recovered only from adjacent soil samples. Sequences related to Pseudomonas, Acidobacteria, and Flexibacter sp. were recovered from both anthrosols and adjacent soils. The strong similarities among the microbial communities present
in the anthrosols for both the Bacteria and Archaea suggests that the microbial community composition in these soils is controlled
more strongly by their historical soil management than by soil type or current land use. The anthrosols had consistently higher
concentrations of incompletely combusted organic black carbon material (BC), higher soil pH, and higher concentrations of
P and Ca compared to their respective adjacent soils. Such characteristics may help to explain the longevity and distinctiveness
of the anthrosols in the Amazonian landscape and guide us in recreating soils with sustained high fertility in otherwise nutrient-poor
soils in modern times. 相似文献
62.
Ismael Galván Eduardo Aguilera Francisco Atiénzar Emilio Barba Guillermo Blanco José L. Cantó Verónica Cortés Óscar Frías István Kovács Leandro Meléndez Anders P. Møller Juan S. Monrós Péter L. Pap Rubén Piculo Juan C. Senar David Serrano José L. Tella Csongor I. Vágási Matthias Vögeli Roger Jovani 《Journal of avian biology》2012,43(3):273-279
Feather mites are arthropods that live on or in the feathers of birds, and are among the commonest avian ectosymbionts. However, the nature of the ecological interaction between feather mites and birds remains unclear, some studies reporting negative effects of feather mites on their hosts and others reporting positive or no effects. Here we use a large dataset comprising 20 189 measurements taken from 83 species of birds collected during 22 yr in 151 localities from seven countries in Europe and North Africa to explore the correlation between feather mite abundance and body condition of their hosts. We predicted that, if wing‐dwelling feather mites are parasites, a negative correlation with host body condition should be found, while a mutualistic interaction should yield positive correlation. Although negative relationships between feather mite abundance and host body condition were found in a few species of birds, the sign of the correlation was positive in most bird species (69%). The overall effect size was only slightly positive (r =0.066). The effect of feather mite abundance explained <10% of variance in body condition in most species (87%). Results suggest that feather mites are not parasites of birds, but rather that they hold a commensalistic relationship where feather mites may benefit from feeding on uropygial gland secretions of their hosts and birds do not seem to obtain a great benefit from the presence of feather mites. 相似文献
63.
An analysis of determinants of amino acids substitution rates in bacterial proteins 总被引:14,自引:0,他引:14
The variation of amino acid substitution rates in proteins depends on several variables. Among these, the protein's expression level, functional category, essentiality, or metabolic costs of its amino acid residues may play an important role. However, the relative importance of each variable has not yet been evaluated in comparative analyses. To this aim, we made regression analyses combining data available on these variables and on evolutionary rates, in two well-documented model bacteria, Escherichia coli and Bacillus subtilis. In both bacteria, the level of expression of the protein in the cell was by far the most important driving force constraining the amino acids substitution rate. Subsequent inclusion in the analysis of the other variables added little further information. Furthermore, when the rates of synonymous substitutions were included in the analysis of the E. coli data, only the variable expression levels remained statistically significant. The rate of nonsynonymous substitution was shown to correlate with expression levels independently of the rate of synonymous substitution. These results suggest an important direct influence of expression levels, or at least codon usage bias for translation optimization, on the rates of nonsynonymous substitutions in bacteria. They also indicate that when a control for this variable is included, essentiality plays no significant role in the rate of protein evolution in bacteria, as is the case in eukaryotes. 相似文献
64.
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66.
Carina Proença Marisa Freitas Daniela Ribeiro Eduardo F. T. Oliveira Joana L. C. Sousa Sara M. Tomé 《Journal of enzyme inhibition and medicinal chemistry》2017,32(1):1216-1228
α-Glucosidase inhibitors are described as the most effective in reducing post-prandial hyperglycaemia (PPHG) from all available anti-diabetic drugs used in the management of type 2 diabetes mellitus. As flavonoids are promising modulators of this enzyme’s activity, a panel of 44 flavonoids, organised in five groups, was screened for their inhibitory activity of α-glucosidase, based on in vitro structure–activity relationship studies. Inhibitory kinetic analysis and molecular docking calculations were also applied for selected compounds. A flavonoid with two catechol groups in A- and B-rings, together with a 3-OH group at C-ring, was the most active, presenting an IC50 much lower than the one found for the most widely prescribed α-glucosidase inhibitor, acarbose. The present work suggests that several of the studied flavonoids have the potential to be used as alternatives for the regulation of PPHG. 相似文献
67.
68.
Land Reform and Land-Use Changes in the Lower Amazon: Implications for Agricultural Intensification 总被引:4,自引:0,他引:4
Land tenure has been considered one of the key factors that define patterns and change in land-use systems. This paper examines the implications of land reform for household decisions regarding patterns of land use, agricultural intensification, and forest conservation. We look at an Amazonian caboclo settlement in the Lower Amazon that had experienced land reform by the end of the 1980s. Results show that defined land tenure is not enough to guarantee agricultural intensification and forest conservation. In fact, several factors working at different scales are affecting land-use change in the region. At the settlement level, privatization of upland forest has led to an overall increase in cultivated land—pasture and annual crops—and increasing deforestation rates. However, at the farm-property level, different systems of agricultural production—intensive, extensive, or abandonment of land—occur according to availability of labor, and capital, and access to different natural resources. 相似文献
69.
70.
Recent Findings in the Chemistry of Odorants from Four Baccharis Species and Their Impact as Chemical Markers 下载免费PDF全文
Manuel Minteguiaga Noelia Umpiérrez Vanessa Xavier Aline Lucas Claudio Mondin Laura Fariña Eduardo Cassel Eduardo Dellacassa 《化学与生物多样性》2015,12(9):1339-1348
Baccharis is a widespread genus belonging to the Asteraceae family that includes almost 400 species exclusively from the Americas. Even when studied in detail, the taxonomic classification among species from this genus is not yet fully defined. Within the framework of our study of the volatile composition of the Baccharis genus, four species (B. trimera, B. milleflora, B. tridentata, and B. uncinella) were collected from the ‘Campos de Cima da Serra’ highlands of the Brazilian state of Rio Grande do Sul. The aerial parts were dried and extracted by the simultaneous distillation extraction (SDE) procedure. This is the first time that SDE has been applied to obtain and compare the volatile‐extract composition in the Baccharis genus. Characterization of the volatile extracts allowed the identification of 180 peaks with many coeluting components; these latter being detailed for the first time for this genus. The multivariate statistical analyses allowed separating the volatile extracts of the four populations of Baccharis into two separate groups. The first one included the B. milleflora, B. trimera, and B. uncinella volatile extracts. The three species showed a high degree of similarity in their volatile composition, which was characterized by the presence of high contents of sesquiterpene compounds, in particular of spathulenol. The second group comprised the extract of B. tridentata, which contained α‐pinene, β‐pinene, limonene, and (E)‐β‐ocimene in high amounts. 相似文献