全文获取类型
收费全文 | 991篇 |
免费 | 56篇 |
专业分类
1047篇 |
出版年
2022年 | 11篇 |
2021年 | 20篇 |
2020年 | 11篇 |
2019年 | 14篇 |
2018年 | 24篇 |
2017年 | 14篇 |
2016年 | 30篇 |
2015年 | 43篇 |
2014年 | 46篇 |
2013年 | 60篇 |
2012年 | 96篇 |
2011年 | 72篇 |
2010年 | 45篇 |
2009年 | 45篇 |
2008年 | 57篇 |
2007年 | 38篇 |
2006年 | 57篇 |
2005年 | 37篇 |
2004年 | 38篇 |
2003年 | 24篇 |
2002年 | 38篇 |
2001年 | 16篇 |
2000年 | 5篇 |
1999年 | 8篇 |
1998年 | 4篇 |
1997年 | 4篇 |
1995年 | 9篇 |
1993年 | 5篇 |
1992年 | 3篇 |
1991年 | 4篇 |
1988年 | 6篇 |
1987年 | 4篇 |
1986年 | 4篇 |
1985年 | 4篇 |
1982年 | 8篇 |
1981年 | 4篇 |
1978年 | 3篇 |
1973年 | 3篇 |
1971年 | 3篇 |
1966年 | 3篇 |
1965年 | 3篇 |
1958年 | 3篇 |
1956年 | 3篇 |
1936年 | 3篇 |
1929年 | 4篇 |
1928年 | 6篇 |
1919年 | 6篇 |
1918年 | 3篇 |
1913年 | 3篇 |
1851年 | 10篇 |
排序方式: 共有1047条查询结果,搜索用时 0 毫秒
81.
Rodriguez-Emmenegger C Kylián O Houska M Brynda E Artemenko A Kousal J Alles AB Biederman H 《Biomacromolecules》2011,12(4):1058-1066
A new route for coating various substrates with antifouling polymer layers was developed. It consisted in deposition of an amino-rich adhesion layer by means of RF magnetron sputtering of Nylon 6,6 followed by the well-controlled, surface-initiated atom transfer radical polymerization of antifouling polymer brushes initiated by bromoisobutyrate covalently attached to amino groups present in the adhesion layer. Polymer brushes of hydroxy- and methoxy-capped oligoethyleneglycol methacrylate and carboxybetaine acrylamide were grafted from bromoisobutyrate initiator attached to a 15 nm thick amino-rich adhesion layer deposited on gold, silicon, polypropylene, and titanium-aluminum-vanadium alloy surfaces. Well-controlled polymerization kinetics made it possible to control the thickness of the brushes at a nanometer scale. Zero fouling from single protein solutions and a reduction of more than 90% in the fouling from blood plasma observed on the uncoated surfaces was achieved. The feasibility of functionalization with bioactive compounds was tested by covalent attachment of streptavidin onto poly(oligoethylene glycol methacrylate) brush and subsequent immobilization of model antibodies and oligonucleotides. The procedure is nondestructive and does not require any chemical preactivation or the presence of reactive groups on the substrate surface. Contrary to current antifouling modifications, the developed coating can be built on various classes of substrates and preserves its antifouling properties even in undiluted blood plasma. The new technique might be used for fabrication of biotechnological and biomedical devices with tailor-made functions that will not be impaired by fouling from ambient biological media. 相似文献
82.
83.
Tomàs Pinós Mario Marotta Eduard Gallardo Isabel Illa Jorge Díaz-Manera Emiliano Gonzalez-Vioque Elena García-Arumí Antoni L. Andreu Ramon Martí 《Mitochondrion》2011,11(1):228-233
We report a heteroplasmic novel mutation m.5636T>C in the mt-tRNAAla in a patient with bilateral ptosis and ophthalmoparesis in whom a muscle biopsy showed cytochrome c oxdidase (COX) negative and ragged red fibers. Using laser capture microdissection we have isolated COX negative fibers and COX positive fibers from the muscle of the patient and determined that the mutation load was clearly increased in COX negative muscle fibers. Additionally, the mutated m.5636T nucleotide is conserved in all the mammal and non-mammal species analyzed and might be structurally relevant as it is located in a position involved in the formation of tertiary structure of canonical mitochondrial tRNAs. 相似文献
84.
Babiychuk EB Atanassoff AP Monastyrskaya K Brandenberger C Studer D Allemann C Draeger A 《PloS one》2011,6(8):e23706
Ceramide is a key lipid mediator of cellular processes such as differentiation, proliferation, growth arrest and apoptosis. During apoptosis, ceramide is produced within the plasma membrane. Although recent data suggest that the generation of intracellular ceramide increases mitochondrial permeability, the source of mitochondrial ceramide remains unknown. Here, we determine whether a stress-mediated plasmalemmal pool of ceramide might become available to the mitochondria of apoptotic cells. We have previously established annexin A1--a member of a family of Ca(2+) and membrane-binding proteins--to be a marker of ceramide platforms. Using fluorescently tagged annexin A1, we show that, upon its generation within the plasma membrane, ceramide self-associates into platforms that subsequently invaginate and fuse with mitochondria. An accumulation of ceramide within the mitochondria of apoptotic cells was also confirmed using a ceramide-specific antibody. Electron microscopic tomography confirmed that upon the formation of ceramide platforms, the invaginated regions of the plasma membrane extend deep into the cytoplasm forming direct physical contacts with mitochondrial outer membranes. Ceramide might thus be directly transferred from the plasma membrane to the mitochondrial outer membrane. It is conceivable that this "kiss-of-death" increases the permeability of the mitochondrial outer membrane thereby triggering apoptosis. 相似文献
85.
Dr. Eduard v. Lindemann 《Plant Systematics and Evolution》1873,23(5):165-166
Ohne Zusammenfassung 相似文献
86.
Eduard Eversmann 《Journal of Ornithology》1853,1(4):282-293
Ohne Zusammenfassung 相似文献
87.
Eduard Eversmann 《Journal of Ornithology》1853,1(1):57-64
Ohne Zusammenfassung 相似文献
88.
89.
F. M. Eduard Opel 《Journal of Ornithology》1858,6(3):205-225
Ohne Zusammenfassung 相似文献
90.