全文获取类型
收费全文 | 540篇 |
免费 | 51篇 |
出版年
2023年 | 4篇 |
2022年 | 5篇 |
2021年 | 12篇 |
2020年 | 13篇 |
2019年 | 18篇 |
2018年 | 20篇 |
2017年 | 19篇 |
2016年 | 22篇 |
2015年 | 34篇 |
2014年 | 35篇 |
2013年 | 41篇 |
2012年 | 31篇 |
2011年 | 32篇 |
2010年 | 19篇 |
2009年 | 18篇 |
2008年 | 23篇 |
2007年 | 26篇 |
2006年 | 13篇 |
2005年 | 24篇 |
2004年 | 16篇 |
2003年 | 16篇 |
2002年 | 18篇 |
2000年 | 5篇 |
1998年 | 3篇 |
1991年 | 5篇 |
1990年 | 6篇 |
1989年 | 2篇 |
1988年 | 6篇 |
1987年 | 2篇 |
1986年 | 8篇 |
1985年 | 3篇 |
1984年 | 6篇 |
1983年 | 2篇 |
1982年 | 5篇 |
1981年 | 4篇 |
1980年 | 3篇 |
1979年 | 5篇 |
1978年 | 3篇 |
1977年 | 5篇 |
1976年 | 3篇 |
1975年 | 4篇 |
1974年 | 3篇 |
1973年 | 10篇 |
1972年 | 5篇 |
1967年 | 2篇 |
1966年 | 2篇 |
1955年 | 3篇 |
1947年 | 2篇 |
1936年 | 6篇 |
1935年 | 2篇 |
排序方式: 共有591条查询结果,搜索用时 437 毫秒
51.
Lisa M. Landino Carolyn M. Brown Carolyn A. Edson Laura J. Gilbert Nathan Grega-Larson Anna Jean Wirth Kelly C. Lane 《Analytical biochemistry》2010,402(1):102-270
Numerous studies of S-glutathionylation of cysteine thiols indicate that this protein modification plays a key role in redox regulation of proteins. To facilitate the study of protein S-glutathionylation, we developed a synthesis and purification to produce milligram quantities of fluorescein-labeled glutathione. The amino terminus of the glutathione tripeptide reacted with fluorescein isothiocyanate readily in ammonium bicarbonate. Purification by solid phase extraction on C8 and C18 columns separated excess reactants from desired products. Both oxidized and reduced fluorescein-labeled glutathione reacted with a variety of thiol-containing proteins to yield fluorescent proteins. 相似文献
52.
53.
54.
Iserhard CA Kaminski LA Marchiori MO Teixeira EC Romanowski HP 《Neotropical Entomology》2007,36(4):612-615
Lepidopterism by Hylesia nigricans (Berg) moth is recorded for the first time in southern Brazil. Preventive strategies of control are proposed based on information on the biology and ecology of this moth. 相似文献
55.
56.
57.
Tramadol (T) is available as a racemic mixture of (+)‐trans‐T and (−)‐trans‐T. The main metabolic pathways are O‐demethylation and N‐demethylation, producing trans‐O‐desmethyltramadol ( M1 ) and trans‐N‐desmethyltramadol ( M2 ) enantiomers, respectively. The analgesic effect of T is related to the opioid activity of (+)‐trans‐T and (+)‐ M1 and to the monoaminergic action of (+/−)‐trans‐T. This is the first study using tandem mass spectrometry as a detection system for the simultaneous analysis of trans‐T, M1 , and M2 enantiomers. The analytes were resolved on a Chiralpak® AD column using hexane:ethanol (95.5:4.5, v/v) plus 0.1% diethylamine as the mobile phase. The quantitation limits were 0.5 ng/ml for trans‐T and M1 and 0.1 ng/ml for M2 . The method developed and validated here was applied to a pharmacokinetic study in rats. Male Wistar rats (n = 6 at each time point) received a single oral dose of 20 mg/kg racemic trans‐T. Blood samples were collected up to 12 h after drug administration. The kinetic disposition of trans‐T and M2 was enantioselective (AUC(+)/(−) ratio = 4.16 and 6.36, respectively). The direction and extent of enantioselectivity in the pharmacokinetics of trans‐T and M2 in rats were comparable to data previously reported for healthy volunteers, suggesting that rats are a suitable model for enantioselective studies of trans‐T pharmacokinetics. Chirality, 2011. © 2010 Wiley‐Liss, Inc. 相似文献
58.
59.
60.