全文获取类型
收费全文 | 1504篇 |
免费 | 124篇 |
专业分类
1628篇 |
出版年
2023年 | 22篇 |
2022年 | 30篇 |
2021年 | 45篇 |
2020年 | 46篇 |
2019年 | 50篇 |
2018年 | 57篇 |
2017年 | 65篇 |
2016年 | 76篇 |
2015年 | 85篇 |
2014年 | 94篇 |
2013年 | 114篇 |
2012年 | 119篇 |
2011年 | 110篇 |
2010年 | 69篇 |
2009年 | 50篇 |
2008年 | 83篇 |
2007年 | 58篇 |
2006年 | 63篇 |
2005年 | 54篇 |
2004年 | 47篇 |
2003年 | 48篇 |
2002年 | 43篇 |
2001年 | 18篇 |
2000年 | 26篇 |
1999年 | 17篇 |
1998年 | 4篇 |
1997年 | 5篇 |
1996年 | 7篇 |
1993年 | 4篇 |
1992年 | 6篇 |
1991年 | 5篇 |
1990年 | 8篇 |
1989年 | 5篇 |
1988年 | 10篇 |
1987年 | 3篇 |
1986年 | 7篇 |
1985年 | 3篇 |
1984年 | 8篇 |
1983年 | 3篇 |
1982年 | 4篇 |
1981年 | 6篇 |
1979年 | 5篇 |
1978年 | 3篇 |
1977年 | 3篇 |
1973年 | 4篇 |
1966年 | 2篇 |
1955年 | 3篇 |
1947年 | 2篇 |
1936年 | 6篇 |
1935年 | 2篇 |
排序方式: 共有1628条查询结果,搜索用时 15 毫秒
211.
Bruno A. Aparício José Cascalho Maria J. Cruz Paulo A. V. Borges Eduardo B. Azevedo Rui B. Elias Fernando Ascensão 《Journal of Insect Conservation》2018,22(2):257-265
There is a vast body of literature aiming to predict, for a large number of taxa, the spatial distribution of suitable areas given the expected future changes of climatic conditions. However, such studies often overlook the role of landscape functional connectivity. This is particularly relevant for species with low vagility, as ground-dwelling insects, inhabiting areas with high human pressure due to habitat destruction and fragmentation, namely in the islands. In this study, we developed an individual-based model (IBM) that simulates individual movement according to landscape resistance and mortality probability, in order to derive the landscape movement map, and applied it to five endemic ground-dwelling insects of Terceira Island (Azores). We then confronted the movement maps of each species against the species distribution models previously developed for both current and future climatic conditions, quantifying the amount of important movement areas that are enclosed by the distribution polygons. We further sought to identify where habitat restoration would increase the overall connectivity among large habitat patches. Our results showed that, for both timeframes, the distribution models enclosed small amounts of areas predicted to be important for animal movement. Additionally, we predicted strong reductions (up to 94%) of these important areas for functional connectivity. We also identified areas in-between native forest of primary importance for restoration that may significantly increase the probability of persistence of our model species. We anticipate that this study will be useful to both conservation planners and ecologists seeking to understand species movement and dispersal both is islands and elsewhere. 相似文献
212.
Alberto V. Borges Gaëlle Speeckaert Willy Champenois Mary I. Scranton Nathalie Gypens 《Ecosystems》2018,21(4):583-599
Dissolved CH4 concentrations in the Belgian coastal zone (North Sea) ranged between 670 nmol l?1 nearshore and 4 nmol l?1 offshore. Spatial variations of CH4 were related to sediment organic matter (OM) content and gassy sediments. In nearshore stations with fine sand or muddy sediments, the CH4 seasonal cycle followed water temperature, suggesting methanogenesis control by temperature in these OM-rich sediments. In offshore stations with permeable sediments, the CH4 seasonal cycle showed a yearly peak following the chlorophyll-a spring peak, suggesting that in these OM-poor sediments, methanogenesis depended on freshly produced OM delivery. This does not exclude the possibility that some CH4 might originate from dimethylsulfide (DMS) or dimethylsulfoniopropionate (DMSP) or methylphosphonate transformations in the most offshore stations. Yet, the average seasonal CH4 cycle was unrelated to those of DMS(P), very abundant during the Phaeocystis bloom. The annual average CH4 emission was 126 mmol m?2 y?1 in the most nearshore stations (~4 km from the coast) and 28 mmol m?2 y?1 in the most offshore stations (~23 km from the coast), 1260–280 times higher than the open ocean average value (0.1 mmol m?2 y?1). The strong control of CH4 by sediment OM content and by temperature suggests that marine coastal CH4 emissions, in particular in shallow areas, should respond to future eutrophication and warming of climate. This is supported by the comparison of CH4 concentrations at five stations obtained in March 1990 and 2016, showing a decreasing trend consistent with alleviation of eutrophication in the area. 相似文献
213.
214.
Eliane de O. Borges Camila B. C. Martins Rodolfo R. da Silva Paulo H. G. Zarbin 《Arthropod-Plant Interactions》2018,12(1):123-131
In response to injury, plants produce volatile organic compounds (VOCs) that usually differ depending on the type of damage they have suffered (e.g., mechanical damage, herbivory, and oviposition). The objectives of this study were to identify and compare the bouquet of volatiles emitted by passion vine plants (Passiflora edulis) after injury caused by mechanical damage (MD), herbivory (HB), and oviposition (OV) by the lepidopteran, Heliconius erato phyllis. Following injury, extracts of plant emissions were collected from each treatment every 24 h for three days and were analyzed by GC and GC/MS. Results show that plants emitted 12 volatiles before and after damage, namely terpenoids, ketones, and aldehydes. Although no significant differences were detected between the three treatments individually, if the entire bouquet of volatiles is analyzed, samples collected at 24 h were different from samples collected at 48 and 72 h. However, terpenoid emission increased significantly in HB plants after 24 h. HB plants emitted approximately 6300, 50, 46, 11, 6, and 3.6 times more (3E,7E)-4,8,12-trimethyl-1,3,7,11-tridecatetraene, (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT), (E)-β-ocimene, (Z)-β-farnesene, (E)-β-caryophyllene, and farnesane, respectively, compared to control plants. OV plants displayed a peak of emission of (E)-β-ocimene after 72 h, which distinguished them from HB plants. MD plants showed a general increase of VOCs versus undamaged control plants. Furthermore, it has been suggested that (E)-β-ocimene may be sequestered by larvae of H. erato phyllis as a component of the odoriferous bouquet of the abdominal scent glands present in adult males, which play a role in sexual communication. 相似文献
215.
Lorenna Alves Mattos-Moreira Claudia Fortes Ferreira Edson Perito Amorim Carlos Priminho Pirovani Edson Mario de Andrade Mauricio Antônio Coelho Filho Carlos Alberto da Silva Ledo 《Acta Physiologiae Plantarum》2018,40(3):60
Bananas are one of the most important fruits in tropical and subtropical regions worldwide. Each year, banana plantations expand, but the areas available are mostly dry lands. The establishment of strategies for obtaining drought tolerant cultivars depends on understanding of biological responses at genetic, molecular and biochemical levels. Proteomics is a powerful tool for functional characterization of the response of plants to abiotic stress and little is known about drought tolerance in Musa spp. Therefore, the aim of this study was to identify proteins related to drought tolerance in two contrasting banana genotypes, Prata Anã and BRS Tropical, susceptible and tolerant to drought, respectively. Proteins were extracted from rhizomes of bananas grown under greenhouse conditions with control, irrigated and water deficit regimes. The differential protein expression pattern was established by two-dimensional (2-D) electrophoresis and spots analyzed in nano Q-Tof Micro UPLC. Twenty-three differentially expressed proteins were found in the tolerant genotype (BRS Tropical) under water deficit, with proteins involved in metabolism, defense and transport. Proteins were classified according to known function and biosynthetic pathways. Signaling proteins in response to water stress, especially for the biological function of growth and development of plants cells, were also encountered, whereas heat shock proteins played a significant role. This is the first report of proteomic analysis for drought tolerance in ‘Pome’ and ‘Silk-type’ bananas containing the ‘B’ genome. Our work provides insights into Musa spp. response to drought and data for further studies regarding molecular mechanisms, which determine how Musa spp. cells better overcome environmental perturbations. 相似文献
216.
Role of Nγ-Acetyldiaminobutyrate as an Enzyme Stabilizer and an Intermediate in the Biosynthesis of Hydroxyectoine 下载免费PDF全文
David Cnovas Nuno Borges Carmen Vargas Antonio Ventosa Joaquín J. Nieto Helena Santos 《Applied microbiology》1999,65(9):3774-3779
Strain CHR63 is a salt-sensitive mutant of the moderately halophilic wild-type strain Halomonas elongata DSM 3043 that is affected in the ectoine synthase gene (ectC). This strain accumulates large amounts of Nγ-acetyldiaminobutyrate (NADA), the precursor of ectoine (D. Cánovas, C. Vargas, F. Iglesias-Guerra, L. N. Csonka, D. Rhodes, A. Ventosa, and J. J. Nieto, J. Biol. Chem. 272:25794–25801, 1997). Hydroxyectoine, ectoine, and glucosylglycerate were also identified by nuclear magnetic resonance (NMR) as cytoplasmic organic solutes in this mutant. Accumulation of NADA, hydroxyectoine, and ectoine was osmoregulated, whereas the levels of glucosylglycerate decreased at higher salinities. The effect of the growth stage on the accumulation of solutes was also investigated. NADA was purified from strain CHR63 and was shown to protect the thermolabile enzyme rabbit muscle lactate dehydrogenase against thermal inactivation. The stabilizing effect of NADA was greater than the stabilizing effect of ectoine or potassium diaminobutyrate. A 1H NMR analysis of the solutes accumulated by the wild-type strain and mutants CHR62 (ectA::Tn1732) and CHR63 (ectC::Tn1732) indicated that H. elongata can synthesize hydroxyectoine by two different pathways—directly from ectoine or via an alternative pathway that converts NADA into hydroxyectoine without the involvement of ectoine. 相似文献
217.
Ramn Penyalver Amparo García Amparo Ferrer Edson Bertolini María M. Lpez 《Applied microbiology》2000,66(6):2673-2677
The sequence of the gene iaaL of Pseudomonas savastanoi EW2009 was used to design primers for PCR amplification. The iaaL-derived primers directed the amplification of a 454-bp fragment from genomic DNA isolated from 70 strains of P. savastanoi, whereas genomic DNA from 93 non-P. savastanoi isolates did not yield this amplified product. A previous bacterial enrichment in the semiselective liquid medium PVF-1 improved the PCR sensitivity level, allowing detection of 10 to 100 CFU/ml of plant extract. P. savastanoi was detected by the developed enrichment-PCR method in knots from different varieties of inoculated and naturally infected olive trees. Moreover, P. savastanoi was detected in symptomless stem tissues from naturally infected olive plants. This enrichment-PCR method is more sensitive and less cumbersome than the conventional isolation methods for detection of P. savastanoi. 相似文献
218.
Veruska B M Alencar Gerly A C Brito Nylane M N Alencar Ana M S Assreuy Vicente P T Pinto Edson H Teixeira Emmanuel P Souza Henri Debray Ronaldo A Ribeiro Benildo S Cavada 《Biochimie et biologie cellulaire》2005,83(5):659-666
We investigated the effect of Helianthus tuberosus agglutinin (HTA) on neutrophil migration in vivo and in vitro. The role of resident cells in this effect was analyzed. Peritonitis was induced by injecting stimuli into rat (150-200 g) peritoneal cavities, and in vitro neutrophil chemotaxis was performed using a Boyden microchamber. HTA (80, 200, or 500 microg/mL per cavity) induced significant in vivo neutrophil migration (p < 0.05); in vitro assays showed that this lectin also induced neutrophil chemotaxis, an effect inhibited by the incubation of lectin associated with alpha-D(+)-mannose, its specific binding sugar. Depletion of the resident-cell population by peritoneal lavage did not alter HTA-induced neutrophil migration (200 microg/mL per cavity). The opposite strategy, increasing peritoneal macrophages by intraperitoneally injecting rats with thioglycollate, did not enhance the neutrophil migration produced by HTA (200 microg/mL per cavity). In addition, injection of supernatant from HTA-stimulated macrophage culture (300 microg/mL) into rat peritoneal cavities did not induce neutrophil migration. However, reduction of the peritoneal mast-cell population potentiated the neutrophil migration (p < 0.05) induced by HTA (200 microg/mL per cavity). Lectin from H. tuberosus has a direct neutrophil chemotatic effect that is modulated by mast cells. 相似文献
219.
Ana P Valente Fábio C L Almeida Clovis R Nakaie Shirley Schreier Edson Crusca Eduardo M Cilli 《Journal of peptide science》2005,11(9):556-563
The SPPS methodology has continuously been investigated as a valuable model to monitor the solvation properties of polymeric materials. In this connection, the present work applied HRMAS-NMR spectroscopy to examine the dynamics of an aggregating peptide sequence attached to a resin core with varying peptide loading (up to 80%) and solvent system. Low and high substituted BHAR were used for assembling the VQAAIDYING sequence and some of its minor fragments. The HRMAS-NMR results were in agreement with the swelling of each resin, i.e. there was an improved resolution of resonance peaks in the better solvated conditions. Moreover, the peptide loading and the attached peptide sequence also affected the spectra. Strong peptide chain aggregation was observed mainly in highly peptide loaded resins when solvated in CDCl3. Conversely, due to the better swelling of these highly loaded resins in DMSO, improved NMR spectra were acquired in this polar aprotic solvent, thus enabling the detection of relevant sequence-dependent conformational alterations. The more prominent aggregation was displayed by the VQAAIDYING segment and not by any of its intermediary fragments and these findings were also corroborated by EPR studies of these peptide-resins labelled properly with an amino acid-type spin probe. 相似文献
220.