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101.
Muscle larvae of Trichinella isolates from two outbreaks in Korea were analyzed by a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and multiple-PCR. All of the muscle larvae showed a band similar to that of T. spiralis larvae of the reference strain. The two Korean Trichinella isolates (isolate code ISS623 and ISS1078) might be classifiable to Trichinella spiralis. 相似文献
102.
Summary This paper is focused on the facies associations of an Early-Middle Norian stratigraphic succession cropping outin the Northern
Calabria (Buonvicino, Cosenza). These carbonate deposits, pertaining to the Verbicaro Unit, represent a dolomitized platform
characterized by a clear dominance of automicrites over skeletal metazoans.
On the basis of the chronostatigraphic data and sedimentary evolution, two stratigraphic units have been distinguished: the
Lower Unit (object of the present paper) and the Upper Unit. The Lower Unit consists of an Early-Middle Norian high-relief
prograding carbonate platform. The Upper Unit is represented by Middle p.p.-Upper Norian basinal deposits.
Three main facies associations, indicative of different depositional settings, have been identified.
The inner platform facies association is characterized by automicrite (cauliflower-columnar subordinate planar stromatolites)
associated with detrital carbonates (intraclastic breccia, bioclastic grainstone and packstone). Apeculiar facies is represented
by megalodontid-bearing beds. Mud-cracked horizons and low-developed teepees occur quite frequently.
The margin facies association is dominated by automicrite (planar to low relief stromatolites associated with thrombolitic
fenestral boundstone) and detrital carbonates with subaerial exposure features.
The slope facies association includes detrital carbonates (breccia/megabreccia) associated with serpulid/sphinctozoan bioconstructions
and automicrite (planar stromatolites/thrombolitic boundstone).
The whole carbonate body is completely dolomitized; nevertheless the morphology and microarchitecture of carbonate components
(cements, grains and automicrite) is still clearly recognizable. The dolomite Mg content ranges from 40 to 48 mole%, sometimes
reaching the stoichiometric value.
Cements, primary and late, represent a minor component of the rock volume; they occur more frequently on the margin and upper
slope setting. Quantitative tacies analyses lead to the following conclusions:
The sedimentary facies and the low-diversity fossil associations seem to indicate a stressed marine environment; the platform
margin probably flanked an intra-platform basin, not an open marine basin. 相似文献
– | ⊙ the entire platform is largely led by automicrite (50% in the inner platform, 70% in the margin, and 30% in the slope), with planar to columnar stromatolitic or thrombolitic fabrics; |
– | ⊙ primary skeletal framework is usually missing, although small bioconstructions of sphinctozoans and, subordinately, serpulids, occur on the slope; |
– | ⊙ automicrite has been affected by early cementation, as witnessed by non-gravitative microfabric, early fracturing and boring. |
103.
104.
Francini G Scardino A Kosmatopoulos K Lemonnier FA Campoccia G Sabatino M Pozzessere D Petrioli R Lozzi L Neri P Fanetti G Cusi MG Correale P 《Journal of immunology (Baltimore, Md. : 1950)》2002,169(9):4840-4849
Parathyroid hormone-related protein (PTH-rP), a protein produced by prostate carcinoma and other epithelial cancers, is a key agent in the development of bone metastases. We investigated whether the protein follows the self-tolerance paradigm or can be used as a target Ag for anticancer immunotherapy by investigating the immunogenicity of two HLA-A(*)02.01-binding PTH-rP-derived peptides (PTR-2 and -4) with different affinity qualities. PTH-rP peptide-specific CTL lines were generated from the PBMC of two HLA-A(*)02.01(+) healthy individuals, stimulated in vitro with PTH-rP peptide-loaded autologous dendritic cells and IL-2. The peptide-specific CTLs were able to kill PTH-rP(+)HLA-A(*)02.01(+) breast and prostate carcinoma cell lines. The two peptides were also able to elicit a strong antitumor PTH-rP-specific CTL response in HLA-A(*)02.01 (HHD) transgenic mice. The vaccinated mice did not show any sign of side effects due to cell-mediated autoimmunity or toxicity. In this study we describe two immunogenic and toxic-free PTH-rP peptides as valid candidates for the design of peptide-based vaccination strategies against prostate cancer and bone metastases from the most common epithelial malignancies. 相似文献
105.
Pier L. Biagi Alessandra Bordoni Silvana Hrelia Michele Celadon Edoardo Turchetto 《The Journal of nutritional biochemistry》1993,4(12):690-694
Polyenylphosphatidylcholine is a choline-glycerophospholipid containing up to 80% of total fatty acids as linoleic acid and may be an important factor in ensuring normal functioning of cell membranes. We tested the effect of a polyenylphosphatidylcholine-supplemented diet and compared it with both a trilinolein-supplemented and a laboratory chow diet on the fatty acid composition, microviscosity, and delta-6-desaturase activity of liver microsomal membranes of 12-month-old rats, in the absence or presence of oxidative stress induced by adriamycin. Polyenylphosphatidylcholine- and trilinolein-supplemented diets showed a similar increase in linoleic acid content and delta-6-desaturase activity in liver microsomes, indicating that low amounts of linoleic acid are able to partially restore the enzyme activity in old rats, independent of the source of linoleic acid. After adriamycin treatment, delta-6-desaturase activity increased in polyenylphosphatidylcholine and trilinolein groups, indicating a protective mechanism against the damage induced by polyunsaturated fatty acid peroxidation. The measurement of malondialdehyde production showed a protective effect on adriamycin-induced lipid peroxidation by polyenylphosphatidylcholine supplementation only. Microsomal membrane microviscosity did not change independent of diet and adriamycin treatment, suggesting that the response of microsomes to lipid peroxidation might be the maintenance of a given membrane order. Administration of polyenylphosphatidylcholine can prevent or minimize the liver damage induced by adriamycin treatment. 相似文献
106.
Emilia Bramanti Edoardo Benedetti Claudio Nicolini Tatiana Berzina Victor Erokhin Aldo D'Alessio Enzo Benedetti 《Biopolymers》1997,42(2):227-237
A qualitative and quantitative analysis of the conformation of Langmuir-Blodgett (LB) dried films of cytochrome C on silicon wafers was performed by Fourier transform ir (FTIR) spectroscopy. A deconvolution procedure was applied to the amide I band analysis, in order to determine the percentage of the different secondary structures. Qualitative analysis was performed by examining difference spectra. Films obtained by spreading protein solutions at pH 7.4 and 1, dried at 25 and 100°C, on silicon wafers were also examined in order to detect spectral components associated with denatured protein domains, and to compare them with cytochrome C LB films. FTIR spectroscopy showed that the following important changes characterise LB film spectra: (a) the α-helix component is higher (its percentage is 57 and 54%) than the one estimated in dried film obtained by spreading the solutions at pH 7.4 on a silicon substrate (43%), (b) there is an increase in the intensity of bands attributed to protonated carboxy group bands, involved and not involved in the formation of hydrogen bonds, and a decrease in those attributed to deprotonated carboxy groups, (c) the intensity of several bands attributed to aromatic amino acids and aliphatic chains increases, and (d) bands due to O(SINGLEBOND)H stretching vibrations of crystallization water are present. These conformational changes could be induced by protein-protein interaction caused by the close packing of molecules that occurs during LB film formation; it cannot be excluded that they may be accompanied by partial changes in the tertiary structure of the protein. A preferential orientation of protein molecules in LB films is also a possibility. © 1997 John Wiley & Sons, Inc. Biopoly 42: 227–237, 1997 相似文献
107.
Emilia Bramanti Edoardo Benedetti Andrea Sagripanti Federico Papineschi Enzo Benedetti 《Biopolymers》1997,41(5):545-553
The secondary structure of human fibrin from normal donors and from bovine and suilline plasma was studied by Fourier transform ir spectroscopy and a quantitative analysis of its secondary structure was suggested. For this purpose, a previously experimented spectrum deconvolution procedure based on the use of the Conjugate Gradient Minimisation Algorithm with the addition of suitable constraints was applied to the analysis of conformation-sensitive amide bands. This procedure was applied to amide I and III analysis of bovine and suilline fibrin, obtained industrially, and to amide III analysis of human fibrin clots. The analysis of both amide I and III in the first case was useful in order to test the reliability of the method. We found bovine, suilline, and human fibrin to contain about 30% α-helix (amide I and III components at 1653 cm−1, and 1312 and 1284 cm−1, respectively), 40% β-sheets (amide I and III components at 1625 and 1231 cm−1, respectively) and 30% turns (amide I and III components at 1696, 1680, 1675 cm−1, and 1249 cm−1, respectively). The precision of the quantitative determination depends on the amount of these structures in the protein. Particularly, the coefficient of variation is < 10% for percentage values of amide I and III components > 15 and 5%, respectively. The good agreement of our quantitative data, obtained separately by amide I and amide III analysis, and consistent with a previous fibrinogen (from commercial sources) study that reports only information about fibrin β-sheet content obtained by factor analysis, leads us to believe that the amounts of secondary structures found (α-helix, β-sheets, and turns) are accurate. © 1997 John Wiley & Sons, Inc. Biopoly 41: 545–553, 1997. 相似文献
108.
Prof. Eleonora Francini 《Plant biosystems》2013,147(2):383-400
Riassunto L'A. prende in considerazione il comportamento del cercine mucillagginoso e del nucleolo durante la divisione cellulare in Oedogonium e prospetta l'ipotesi che il nucleolo sia in relazione con la formazione della membrana trasversale. 相似文献
109.
Involvement of fractalkine and macrophage inflammatory protein-1 alpha in moderate-severe depression
Merendino RA Di Pasquale G De Luca F Di Pasquale L Ferlazzo E Martino G Palumbo MC Morabito F Gangemi S 《Mediators of inflammation》2004,13(3):205-207
Moderate-severe depression (MSD) is linked to overexpression of proinflammatory cytokines and chemokines. Fractalkine (FKN) and macrophage inflammatory protein-1 alpha (MIP-1alpha) are, respectively, members of CX3C and C-C chemokines, and both are involved in recruiting and activating mononuclear phagocytes in the central nervous system. We analysed the presence of FKN and MIP-1alpha in sera of untreated MSD patients and healthy donors. High FKN levels were observed in all MSD patients as compared with values only detectable in 26% of healthy donors. MIP-1alpha was measurable in 20% of patients, while no healthy donors showed detectable chemokine levels. In conclusion, we describe a previously unknown involvement of FKN in the pathogenesis of MSD, suggesting that FKN may represent a target for a specific immune therapy of this disease. 相似文献
110.
Lalle M Pozio E Capelli G Bruschi F Crotti D Cacciò SM 《International journal for parasitology》2005,35(2):207-213
Human giardiasis, caused by the intestinal flagellate Giardia duodenalis, is considered a zoonotic infection, although the role of animals in the transmission to humans is still unclear. Molecular characterisation of cysts of human and animal origin represents an objective means to validate or reject this hypothesis. In the present work, cysts were collected in Italy from humans (n=37) and animals (dogs, one cat and calves, n=46), and were characterised by PCR amplification and sequencing of the beta-giardin gene. As expected, only Assemblages A and B were identified among human isolates. The host-specific Assemblages C and D were found in the majority of dog isolates; however, 6 dog isolates were typed as Assemblage A. The cat-specific Assemblage F has been identified in the single feline isolate available. Among calf isolates, most were typed as Assemblages A (n=12) and B (n=5), whereas the host-specific Assemblage E was rarely found (n=3). Sequence heterogeneity in the beta-giardin gene allowed a number of subgenotypes to be identified within Assemblage A (8 subgenotypes), B (6 subgenotypes), D (2 subgenotypes), and E (3 subgenotypes). Five of these subgenotypes, namely A1, A2, A3, A4 and B3, were found to be associated with infections of humans, of dogs and of calves; these data, therefore, supported the role of these animals as a source of infection for humans. 相似文献