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51.
Sexual networks: implications for the transmission of sexually transmitted infections 总被引:1,自引:0,他引:1
The structures of sexual networks are essential for understanding the dynamics of sexually transmitted infections. Standard epidemiological models largely disregard the complex patterns of intimate contacts. Social network analysis offers important insight into how to conceptualize and model social interaction and has the potential to greatly enhance the understanding of disease epidemics. 相似文献
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Paulo Breinis Flavio Geraldes Alves Camila AE Alves Rafael G Cintra Débora Almeida Priscila C Passarelli Camila Domingues Talita Gerbim Régia Gasparetto Luiz Carlos de Abreu Vitor E Valenti Adriana Gonçalves de Oliveira Carlos Bandeira de Mello Monteiro Rubens Wajnzstejn 《BMC neurology》2014,14(1):1-4
Background
The Mulvihill-Smith Syndrome was first recognized in 1975. After the recognition of the Mulvihill-Smith Syndrome, ten cases have been described.Case presentation
This article describes the eleventh case of this syndrome in a male patient, 24 years-old with short stature and microcephaly with mild cognitive impairment, deafness and allergic conjunctivitis. The patient was hospitalized several times for repeated infections, and the presence of multiple melanocytic nevi on his skin was noticed.Conclusions
Based on the entire set of signs and symptoms presented in our study, it was diagnosed the patient with Mulvihill-Smith Syndrome. 相似文献54.
Philipp Diebolder Armin Keller Stephanie Haase Anne Schlegelmilch Jonathan D Kiefer Tamana Karimi Tobias Weber Gerhard Moldenhauer Roland Kehm Anna M Eis-Hübinger Dirk J?ger Philippe A Federspil Christel Herold-Mende Gerhard Dyckhoff Roland E Kontermann Michaela AE Arndt Jürgen Krauss 《MABS-AUSTIN》2014,6(1):130-142
The development of efficient strategies for generating fully human monoclonal antibodies with unique functional properties that are exploitable for tailored therapeutic interventions remains a major challenge in the antibody technology field. Here, we present a methodology for recovering such antibodies from antigen-encountered human B cell repertoires. As the source for variable antibody genes, we cloned immunoglobulin G (IgG)-derived B cell repertoires from lymph nodes of 20 individuals undergoing surgery for head and neck cancer. Sequence analysis of unselected “LYmph Node Derived Antibody Libraries” (LYNDAL) revealed a naturally occurring distribution pattern of rearranged antibody sequences, representing all known variable gene families and most functional germline sequences. To demonstrate the feasibility for selecting antibodies with therapeutic potential from these repertoires, seven LYNDAL from donors with high serum titers against herpes simplex virus (HSV) were panned on recombinant glycoprotein B of HSV-1. Screening for specific binders delivered 34 single-chain variable fragments (scFvs) with unique sequences. Sequence analysis revealed extensive somatic hypermutation of enriched clones as a result of affinity maturation. Binding of scFvs to common glycoprotein B variants from HSV-1 and HSV-2 strains was highly specific, and the majority of analyzed antibody fragments bound to the target antigen with nanomolar affinity. From eight scFvs with HSV-neutralizing capacity in vitro, the most potent antibody neutralized 50% HSV-2 at 4.5 nM as a dimeric (scFv)2. We anticipate our approach to be useful for recovering fully human antibodies with therapeutic potential. 相似文献
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Roosa AE Laitinen Suvi Broholm Victor A Albert Teemu H Teeri Paula Elomaa 《BMC plant biology》2006,6(1):11-18
Background
The inflorescence of the cut-flower crop Gerbera hybrida (Asteraceae) consists of two principal flower types, ray and disc, which form a tightly packed head, or capitulum. Despite great interest in plant morphological evolution and the tractability of the gerbera system, very little is known regarding genetic mechanisms involved in flower type specification. Here, we provide comparative staging of ray and disc flower development and microarray screening for differentially expressed genes, accomplished via microdissection of hundreds of coordinately developing flower primordia. 相似文献57.
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Pre-emptive targeting of the epitope spreading cascade with genetically modified regulatory T cells during autoimmune demyelinating disease. 总被引:1,自引:0,他引:1
L Yin M Yu A E Edling J A Kawczak P M Mathisen T Nanavati J M Johnson V K Tuohy 《Journal of immunology (Baltimore, Md. : 1950)》2001,167(11):6105-6112
Epitope spreading or endogenous self-priming has been implicated in mediating the progression of autoimmune disease. In the present study we created an immune-deviated, epitope spreading response in SWXJ mice after the onset of experimental autoimmune encephalomyelitis, a prototypic autoimmune animal model widely used in multiple sclerosis research. We established an immunoregulatory spreading repertoire by transferring T cells genetically modified to produce high levels of IL-10 in response to a dominant epitope spreading determinant. Installation of a Th2/Tr1-like spreading repertoire resulted in a marked and prolonged inhibition of disease progression and demyelination characterized by 1) bystander inhibition of the recall response to the priming immunogen, and 2) a Th1-->Tr1 immune-deviated spreading response involving a shift in the source of IL-10 production from the transferred regulatory population to the host-derived, endogenously primed repertoire. Thus, our data provide a rationale for cell-based therapeutic intervention in multiple sclerosis by showing that pre-emptive targeting of the epitope spreading cascade with regulatory T cells effectively induces an immune-deviated spreading response capable of inhibiting ongoing inflammatory autoreactivity and disease progression. 相似文献