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31.
Summary Enzymes involved in flavonoid synthesis in parsley (Petroselinum nortense Hoffm.) cell suspension cultures are induced by light. It has been suggested that the regulation of the enzymes of phenylpropanoid metabolism (group 1) is independent of that of the enzymes of flavonoid synthesis proper (group 2) [Hahl-brock, K., Ebel, J., Ortmann, R., Sutter, A., Wellmann, E., Grisebach, H.: Biochim. biophys. Acta (Amst.) 244, 7–15 (1971)]. Phytochrome was demonstrated to be involved in the light effect controlling flavonoid synthesis. Phytochrome is only effective after a preceding irradiation with ultraviolet light (max<300 nm) [Wellmann, E.: Planta (Berl.) 101, 283–286 (1971)]. — In order to determine whether phytochrome affects phenylalanine ammonia-lyase (PAL; EC 4.3.1.5), a group 1 enzyme, or exerts a general effect of all enzymes of the flavonoid pathway. PAL and two enzymes from group 2, chalcone-flavanone isomerase and UDP-apiose synthetase, were investigated. Under appropriate conditions of irradiation (low UV-dose with subsequent red/far-red pulses) both group 2 enzymes were shown to be controlled by phytochrome to the same extent as PAL. The UV-induced increase in activity of all three enzymes is reduced to about 70% by an irradiation for 10 min with far-red light. This far-red effect is fully reversible by a subsequent irradiation with 10 mith red light. There is no evidence for a rate limiting enzyme (or group of enzymes) involved in flavonoid formation with respect to phytochrome control.
Abkürzungen PAL Phenylalaninammoniumlyasc - CFI Chalkonflavanonisomerase 相似文献
Abkürzungen PAL Phenylalaninammoniumlyasc - CFI Chalkonflavanonisomerase 相似文献
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33.
Phytochrome-induced increases in enzyme activities for phenylalanine ammonia-lyase (EC 4.3.1.5) and chalcone isomerase (EC 5.5.1.6), and in amounts of the related end products, anthocyanin and the flavonol, quercetin, were measured in cotyledons of mustard (Sinapis alba L.). There was no correlation between the activities of these enzymes and the rate of anthocyanin accumulation; however, some correlation was found with the quercetin accumulation rate. Since anthocyanin and flavonol accumulation is spatially separated in mustard (flavonols in the upper epidermis, anthocyanin in the lower epidermis), it was possible to measure anthocyanin-associated phenylalanine ammonia-lyase independently. This activity correlated well with the accumulation rate for anthocyanin during the first few hours after induction. The phytochrome effect on anthocyanin formation differed from that on quercetin formation: anthocyanin was strongly induced by continuous far-red light and by both continuous red light and red light pulses, whereas quercetin was only effectively induced by continuous far-red light.Abbreviations CHI
chalcone isomerase
- PAL
phenylalanine ammonia-lyase 相似文献
34.
Summary A comparison was made of the activity of carboxydismutase (ribulose-1,5-diphosphate carboxylase) between higher plant species which possess the -carboxylation (C4-dicarboxylic acid) pathway for photosynthesis and species which lack this pathway. Contrary to earlier findings no marked difference in the level of this enzyme was found between the two groups of species. Chloroplast-containing vascular-bundle-sheath cells which seem to be present only in plants with -carboxylation apparently contain relatively high carboxydismutase activity.C.I.W.-D.P.B. Publ. No. 453. 相似文献
35.
Defective Interfering Particles of Poliovirus I. Isolation and Physical Properties 总被引:34,自引:22,他引:12
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A class of defective interfering (DI) poliovirus particles has been identified. The first was found as a contaminant of a viral stock; others have been isolated by serial passage at a high multiplicity of infection. The DI particles are less dense than standard virus and sediment more slowly. Their ribonucleic acid (RNA) sediments more slowly than standard RNA and has a higher electrophoretic mobility. Competition hybridization experiments with double-stranded viral RNA indicate that DI RNA is 80 to 90% of the length of standard RNA. The proteins of DI particles are indistinguishable from those of standard poliovirus. 相似文献
36.
Axel Ruhland Eckard Haase Wolfram Siede Martin Brendel 《Molecular & general genetics : MGG》1981,181(3):346-351
Summary Mutants of Saccharomyces cerevisiae with enhanced sensitivity to the DNA cross-linking agent nitrogen mustard (HN2) have been isolated and partially characterized with respect to their phenotypic and genetic properties. The screening technique, based on HN2-sensitivity as sole criterion, yields approximately 1 sensitive isolate in 200 clones when applied to an intensively mutagenized population of a resistant parent strain. Mutants characterized so far are all due to recessive nuclear genes and represent at least seven complementation groups. They exhibit different degrees as well as different patterns of sensitivity towards monofunctional and bifunctional alkylating agents, and ultraviolet light. 相似文献
37.
38.
Background
Adverse drug reactions (ADRs) are now recognized as an important cause of hospital admissions, with a proportion ranging from 0.9–7.9%. They also constitute a significant economic burden. We thus aimed at determining the prevalence and the economic burden of ADRs presenting to Medical Emergency Department (ED) of a tertiary referral center in India 相似文献39.
Pincus-Knackstedt MK Joachim RA Blois SM Douglas AJ Orsal AS Klapp BF Wahn U Hamelmann E Arck PC 《Journal of immunology (Baltimore, Md. : 1950)》2006,177(12):8484-8492
Allergic asthma is one of the most prevalent and continuously increasing diseases in developed countries. Its clinical features include airway hyperresponsiveness and inflammation upon allergen contact. Furthermore, an emerging area of research subsumed as fetal programming evaluates the impact of environmental insults in utero on the incidence of diseases in later life. The aim of this study was to identify whether prenatal exposure to stress, which constitutes a severe environmental insult, perpetuates airway inflammation in later life. Our experiments were performed in mice and revealed that prenatally stressed adult offspring indeed show an increased vulnerability toward airway hyperresponsiveness and inflammation. Furthermore, we provide persuasive insights on dysregulated pathways of the cellular and humoral immune response upon Ag challenge in prenatally stressed adult offspring, reflected by a Th2 greater Th1 adaptive immune response and increased CCR3 and IgE levels in vivo. Additionally, APCs derived from prenatally stressed offspring trigger clonal expansion of Th2 cells in vitro. We also deliver experimental evidence for a reduced corticotrophin-releasing hormone expression in the paraventricular nucleus of adult offspring in response to prenatal stress. Furthermore, behavioral analyses indicate an increase in anxiety in these mice. In conclusion, our data will facilitate future research aiming to identify the individual impact, hierarchy, and redundancy of multiple key protagonists in airway inflammation in an interdisciplinary context. This will foster the substantiation of disease-prevention strategies, such as asthma, during the prenatal period. 相似文献
40.
Chronic exposure to low doses of arsenite causes transformation of human osteogenic sarcoma (HOS) cells. Although oxidative stress is considered important in arsenite-induced cell transformation, the molecular and cellular mechanisms by which arsenite transforms human cells are still unknown. In the present study, we investigated whether altered iron homeostasis, known to affect cellular oxidative stress, can contribute to the arsenite-mediated cell transformation. Using arsenite-induced HOS cell transformation as a model, it was found that total iron levels are significantly higher in transformed HOS cells in comparison to parental control HOS cells. Under normal iron metabolism conditions, iron homeostasis is tightly controlled by inverse regulation of ferritin and transferrin receptor (TfR) through iron regulatory proteins (IRP). Increased iron levels in arsenite transformed cells should theoretically lead to higher ferritin and lower TfR in these cells than in controls. However, the results showed that both ferritin and TfR are decreased, apparently through two different mechanisms. A lower ferritin level in cytoplasm was due to the decreased mRNA in the arsenite-transformed HOS cells, while the decline in TfR was due to a lowered IRP-binding activity. By challenging cells with iron, it was further established that arsenite-transformed HOS cells are less responsive to iron treatment than control HOS cells, which allows accumulation of iron in the transformed cells, as exemplified by significantly lower ferritin induction. On the other hand, caffeic acid phenethyl ester (CAPE), an antioxidant previously shown to suppress As-mediated cell transformation, prevents As-mediated ferritin depletion. In conclusion, our results suggest that altered iron homeostasis contributes to arsenite-induced oxidative stress and, thus, may be involved in arsenite-mediated cell transformation. 相似文献