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51.
Asli F. Ceylan Shuyi Wang Machender R. Kandadi Jie Chen Yinan Hua Zhaohui Pei Sreejayan Nair Jun Ren 《生物化学与生物物理学报:疾病的分子基础》2018,1864(10):3339-3352
Endothelin (ET)-1 is implicated in the pathophysiology of cardiovascular diseases although its role in obesity anomalies has not been fully elucidated. This study was designed to examine the impact of ET-1 receptor A (ETA) ablation on obesity-induced changes in cardiac geometry and contractile function, as well as the mechanisms involved with a focus on autophagy. Cardiomyocyte-specific ETA receptor knockout (ETAKO) and WT mice were fed either low-fat (10% calorie from fat) or high-fat (45% calorie from fat) diet for 24?weeks. Glucose tolerance test was examined to confirm insulin resistance. High-fat diet intake compromised myocardial geometry (enlarged left ventricular diameters in systole and diastole), morphology (cardiac hypertrophy, increased wall thickness and interstitial fibrosis), contractile function (reduced fractional shortening, ejection fraction and cardiomyocyte shortening) and intracellular Ca2+ handling, the effect of which was significantly attenuated by ETAKO. TUNEL staining revealed overt apoptosis in high-fat-fed group, the effect was reverted by ETAKO. Western blot analysis noted that high-fat intake downregulated leptin receptor and PPARγ, insulin signaling (elevated basal/dampened insulin-stimulated phosphorylation of Akt and IRS1), phosphorylation of AMPK, ACC, upregulated GATA-4, ANP, NFATc3, PPARα, m-TOR/p70s6k signaling, which were attenuated by ETAKO with the exception of AMPK/ACC. Furthermore, high-fat intake suppressed cardiac autophagy, which was abrogated by ETAKO. In cultured murine cardiomyocytes, palmitic acid challenged mimicked high-fat diet-induced hypertrophic and autophagic responses, the effect of which were abolished by the ETA receptor antagonist BQ123 or mTOR inhibitor rapamycin. These results suggest that inhibition of ETA rescues high-fat intake-induced cardiac anomalies possibly through autophagy regulation. 相似文献
52.
Sule Ceylan Hacer Bayrak Ahmet Demirbas Serdar Ulker Sengul Alpay-Karaoglu Neslihan Demirbas 《Russian Journal of Bioorganic Chemistry》2014,40(3):314-329
1,2,4-Triazole-3-one prepared from tryptamine was converted to the corresponding carbothioamides by several steps. Their treatment with ethyl bromoacetate or 4-chlorophenacyl bromide produced the corresponding 5-oxo-1,3-thiazolidine or 3-(4-chlorophenyl)-1,3-thiazole derivatives. Acetohydrazide derivative that was obtained starting from tryptamine, was converted to the corresponding Schiff basis and sulfonamide by the treatment with suitable aldehydes and benzensulphonyl chloride, respectively. 2-[(4-Amino-5-thioxo-4,5-dihydro-1H-1,2,4-triazole-3-yl)methyl]-4-[2-(1H-indole-3-yl)ethyl]-5-methyl-2,4-dihydro-3H-1,2,4-triazole-3-one was synthesized starting from hydrazide via the formation of the corresponding 1,3,4-oxadiazole compound, while the other bitriazole compounds were obtained by intramolecular cyclisation of carbothioamides in basic media. The treatment of 1,2,4-triazole or 1,3,4-oxadiazole compound with several amines generated the corresponding Mannich bases. Ethyl (2-amino-1,3-thiazole-4-yl)acetate was converted to the corresponding 1,3,4-oxadiazole derivative, arylidenehydrazides, 1,2,4-triazole-3-one and 5-oxo-1,3-oxazolidine derivatives by several steps. The structural assignments of new compounds were based on their elemental analysis and spectral (FT IR, 1H NMR, 13C NMR and LC-MS) data. The antimicrobial, antilipase and antiurease activity studies revealed that some of the synthesized compounds showed antimicrobial, antilipase and/or antiurease activity. 相似文献
53.
Erman F Aydin S Demir Y Akcay F Bakan E 《Journal of biochemistry and molecular biology》2006,39(5):516-521
Modifications in dietary fatty acid intake might lead to a modification in membrane phospholipid fatty acid composition. The purpose of this study was to investigate relationship between different type of oil consumption and leukocyte membrane phospholipid composition. This study was carried out in subjects utilizing butter (n = 15), margarine (n = 15), fluid oil (n = 15) and mixed types of oils (n = 15) in total 60 subjects. Leukocytes were separated from total blood by dextran sedimentation method. Membrane lipids and proteins were isolated following the cell disruption. Fatty acids of membrane phospholipids were isolated by hydrolysation with phospholipase B under ultrasonic dismembranator. Free fatty acids were identified with gas chromatography at chloroform phase. The results obtained were compared with data obtained by chromatograms of the standards. Results more prominent values of arachidic, dihomo-gamma-linolenic and palmitoleic acids were found in butter-or mixed oil-user groups; eicosadienoic, eicosamonoenoic, dihomo-gamma-linolenic and behenic acids in fluid oil heptanoic, valeric, eicosadienoic and linolenic acids in margarine groups. The fatty acid composition of mixed oil; was similar to butter, while other two oils were so different. From this study, it was concluded that the type of oil consumption might have an influence on phospholipid components of plasma membranes. 相似文献
54.
Kocpinar Enver Fehim Gonul Baltaci Nurdan Ceylan Hamid Kalin Seyda Nur Erdogan Orhan Budak Harun 《Biological trace element research》2020,194(1):135-144
Biological Trace Element Research - This study is aimed at evaluating the effect of dietary zinc-methionine (Zn-Met) supplementation during 3 months prepartum up to 9 months... 相似文献
55.
Ceylan Merve Kına Ümit Yaşar Çakır Özgür Kara Neslihan Turgut 《Plant Cell, Tissue and Organ Culture》2021,146(1):83-95
Plant Cell, Tissue and Organ Culture (PCTOC) - Cycloastragenol (CAG) is a triterpenoid saponin compound that is synthesized by Astragalus species. CAG has many bioactivity, including telomerase... 相似文献
56.
Sadallah S Eken C Martin PJ Schifferli JA 《Journal of immunology (Baltimore, Md. : 1950)》2011,186(11):6543-6552
Microparticles (MP) shed by platelets (PLT) during storage have procoagulant activities, but little is known about their properties to modify inflammation or immunity. In this study, we studied the capacity of MP present in PLT concentrates to alter the function of macrophages and dendritic cells (DC). The size of the purified MP was between 100 and 1000 nm, and they expressed phosphatidylserine; surface proteins of PLT (CD61, CD36, CD47), including complement inhibitors (CD55, CD59), but not CD63; and proteins acquired from plasma (C1q, C3 fragments, factor H). These characteristics suggest that the MP shed by PLT are formed by budding from the cell surface, corresponding to ectosomes. The purified PLT ectosomes (PLT-Ect) reduced the release of TNF-α and IL-10 by macrophages activated with LPS or zymosan A. In addition, PLT-Ect induced the immediate release of TGF-β from macrophages, a release that was not modified by LPS or zymosan A. Macrophages had a reduced TNF-α release even 24 h after their exposure to PLT-Ect, suggesting that PLT-Ect induced a modification of the differentiation of macrophages. Similarly, the conventional 6-d differentiation of monocytes to immature DC by IL-4 and GM-CSF was modified by the presence of PLT-Ect during the first 2 d. Immature DC expressed less HLA-DP DQ DR and CD80 and lost part of their phagocytic activity, and their LPS-induced maturation was downmodulated when exposed to PLT-Ect. These data indicate that PLT-Ect shed by stored PLT have intrinsic properties that modify macrophage and DC differentiation toward less reactive states. 相似文献
57.
The effect of pretreatment of rabbit sperm cells with different concentrations of cholesterol-loaded cyclodextrin (CLC) on the occurrence of premature acrosome reactions during 72 h of liquid storage was investigated in three successive experiments. The aim of the first experiment was to establish a liquid storage model to facilitate premature acrosome reactions in rabbit sperm cells and, therefore, examined the relative effects of different dilution rates (1:5, 1:25 or 1:50) and storage temperatures (4°C or 35°C) on the occurrence of premature acrosome reactions. Increasing both dilution rate (from 1:5 to 1:25; P<0.05) and storage temperature (from 4°C to 35°C; P<0.0001) significantly enhanced the percentage of sperm cells that underwent premature acrosome reactions during storage. Therefore, a constant dilution rate of 1:25 and storage temperature of 35°C was employed for the rest of the study. The second experiment examined the effect of different CLC concentrations (0, 0.5, 1.0 and 3.0mg per 120×10(6) spermatozoa) on the occurrence of premature acrosome reactions in sperm cells. CLC supplementation of the extender inhibited (P<0.001) premature acrosome reactions in sperm cells in a dose-dependent manner during 72 h of storage. In the third experiment, the ability of CLC-pretreated sperm cells to undergo acrosome reactions induced by lysophosphatidylcholine (LPC) was evaluated following 72 h of storage. A considerable proportion of sperm cells pretreated with CLC (between 68.7 and 91.8%) underwent the acrosome reaction in response to LPC following 72 h of liquid storage. However, the ability of the sperm cells to undergo the acrosome reaction varied with regards to the dose levels of CLC pretreatment (P<0.001). In conclusion, CLC supplementation prevents premature acrosome reactions in liquid-stored rabbit spermatozoa. 相似文献
58.
Sonmez F Sevmezler S Atahan A Ceylan M Demir D Gencer N Arslan O Kucukislamoglu M 《Bioorganic & medicinal chemistry letters》2011,21(24):7479-7482
A newly series of 4-(phenylurenyl)chalcone (4a–j) and 4′-(phenylurenyl/thiourenyl)chalcone (9a–l) derivatives were synthesized and their inhibitory effects on the diphenolase activity of banana tyrosinase were evaluated. Tyrosinase has been purified from banana on an affinity gel comprised of Sepharose 4B-l-tyrosine-p-aminobenzoic acid. The result showed that 4a–j inhibited the PPO enzyme activity. Conversely, 9a–h and 9i–l showed activator effect on tyrosinase enzyme activity. 相似文献
59.
Fusun Eroglu Lutfi Yavuz Berit G. Ceylan Funda Yılmaz Erol Eroglu Namik Delibas Mustafa Nazıroğlu 《Cell biochemistry and function》2010,28(3):211-216
It has been well known that some volatile anesthetic agents produce oxidative stress. Desflurane as a new volatile agent might have limited oxidative toxic effect because it is relatively a new short‐acting anesthetic characterized by a short duration of action and a quick postanesthetic recovery. We investigated effect of desflurane on serum glutathione peroxidase (GSH‐Px), lipid peroxidation (LP), vitamin E, and erythrocyte superoxide dismutase (SOD) values in patients. Fifteen adult patients are scheduled for elective surgery, ASA I or II physical status. Tidal volume and ventilation frequency were kept unchanged during the operation. Baseline values in venous blood samples were preoperatively taken and blood was also taken postoperatively at the 1st and the 12th hours of desflurane exposure. LP levels were significantly (p < 0.05) higher postoperatively at 1st hour than in preoperative values while α‐tocopherol concentration was significantly (p < 0.001) lower in postoperative period at 1st hour than in preoperative period. Erythrocyte SOD and serum GSH‐Px activities did not differ between pre‐ and postoperative periods. In conclusion, we observed that desflurane produced oxidative stress by decreasing α‐tocopherol levels. Use of vitamin E may be possible to reduce the oxidative effect of desflurane. Copyright © 2010 John Wiley & Sons, Ltd. 相似文献
60.
Ceylan A Tuncer O Sayin R Peker E Caksen H Sari S 《Genetic counseling (Geneva, Switzerland)》2011,22(1):75-78
Summary: Congenital myasthenic syndromes (CMS) are diseases of the neuromuscular junction. They usually belong to the disease groups that begin in the infantile or childhood period and carry genetic characteristics. The following is important in establishing the diagnosis of this disease: clinical findings, electromyography, genetic tests, determination of serum acetylcholine receptor antibodies. Acetylcholine esterase inhibitor drugs are used in treatment of CMS. A seven-month old male patient was brought to our department with the complaints of difficult breathing, falling of the eyelids and swallowing difficulty. With clinical and laboratory findings, he was diagnosed with congenital myasthenia and treatment was started. CMS should be suspected in patients with no pathological findings on the physical examination, and normal chest X-rays. 相似文献