A novel Brucella isolate in association with two cases of stillbirth in non-human primates – first report

Background  Brucellosis is veterinary and human health problem.
Methods  A 13-year-old wild caught multiparous and an 8-year-old colony-born nulliparous baboon had stillbirths in the second trimester of pregnancy. Culture isolates from both postpartum uteruses were characterized using traditional biochemical analysis, PCR, and multilocus sequencing.
Results  The isolates morphologically resembled Brucella although their phenotypic characteristics were not consistent with any currently described species. The isolates represent a novel lineage within the genus with unique alleles, not previously seen in surveys of greater than 300 isolates representing the known diversity of the genus, present at 5/9 loci examined.
Conclusions  The described cases are to the best of our knowledge the first presentation of a naturally acquired Brucella infection in non-human primates associated with stillbirths from the same colony where Brucella seropositivity in the baboons was described 45 years ago. The organism appears to represent a previously undescribed Brucella species.     

Closing the gap between science and management of cold-water refuges in rivers and streams

Human activities and climate change threaten coldwater organisms in freshwater ecosystems by causing rivers and streams to warm, increasing the intensity and frequency of warm temperature events, and reducing thermal heterogeneity. Cold-water refuges are discrete patches of relatively cool water that are used by coldwater organisms for thermal relief and short-term survival. Globally, cohesive management approaches are needed that consider interlinked physical, biological, and social factors of cold-water refuges. We review current understanding of cold-water refuges, identify gaps between science and management, and evaluate policies aimed at protecting thermally sensitive species. Existing policies include designating cold-water habitats, restricting fishing during warm periods, and implementing threshold temperature standards or guidelines. However, these policies are rare and uncoordinated across spatial scales and often do not consider input from Indigenous peoples. We propose that cold-water refuges be managed as distinct operational landscape units, which provide a social and ecological context that is relevant at the watershed scale. These operational landscape units provide the foundation for an integrated framework that links science and management by (1) mapping and characterizing cold-water refuges to prioritize management and conservation actions, (2) leveraging existing and new policies, (3) improving coordination across jurisdictions, and (4) implementing adaptive management practices across scales. Our findings show that while there are many opportunities for scientific advancement, the current state of the sciences is sufficient to inform policy and management. Our proposed framework provides a path forward for managing and protecting cold-water refuges using existing and new policies to protect coldwater organisms in the face of global change.     

Ambulatory cassette EEG in epilepsy diagnosis

The electroencephalographic evaluation of patients with possible or proven epilepsy is no longer limited to routine laboratory EEGs or intensive inpatient monitoring. Expanded temporal sampling of the EEG, which increases the probability of documenting, characterizing, and quantitating the electrographic manifestations of these illnesses, is now available on a portable, outpatient, and less cumbersome inpatient basis by means of ambulatory cassette recordings. The technological advances which have made this technique feasible include small multi-channel tape recorders, miniature preamplifiers, and rapid video/audio playback units. New designs in montages and analysis techniques have made the procedure practical. Clinical series and controlled trials have confirmed the usefulness of cassette EEG monitoring in the evaluation of epilepsy and a wide range of other paroxysmal neurologic disorders. Ambulatory EEG diagnostic yields have been shown to be superior to routine laboratory studies and nearly as good as inpatient telemetry evaluations. The role of cassette recordings in clinical electroencephalography continues to be defined as new applications are established.     

Thymic control of secretory antibody responses in the rat.

The effect of neonatal thymectomy on salivary and serum antibody responses was studied in rats. Local immunization of thymectomized rats with a T-dependent antigen (DNPBGG) elicited negligible amounts of IgA anti-DNP antibody in saliva. In contrast, both normal and sham-thymectomized animals demonstrated substantial levels of salivary IgA antibody. All thymectomized rats locally injected with a T-independent antigen (DNP-Lys-Ficoll) exhibited salivary IgA antibody production. Salivary IgG antibodies were somewhat decreased in thymectomized rats injected with either antigen; however, the final effect of T cell deprivation on IgG synthesis was not as pronounced as on IgA synthesis. Serum IgA antibody was induced in control rats injected with DNPBGG, whereas this Ig class of antibody was absent in thymectomized rats. The results suggest that thymus-derived cells exert a regulatory influence on both serum and secretory IgA responses to antigens.     

Screening Global Positioning System Location Data for Errors Using Animal Movement Characteristics

Abstract: Animal locations estimated by Global Positioning System (GPS) inherently contain errors. Screening procedures used to remove large positional errors often trade data accuracy for data loss. We developed a simple screening method that identifies locations arising from unrealistic movement patterns. When applied to a large data set of moose (Alces alces) locations, our method identified virtually all known errors with minimal loss of data. Thus, our method for screening GPS data improves the quality of data sets and increases the value of such data for research and management.     

Host‐plant genotype mediates supply and demand of animal food in an omnivorous insect

1. Omnivorous predators can protect plants from herbivores, but may also consume plant material themselves. Omnivores and their purely herbivorous prey have previously been thought to respond similarly to host‐plant quality. However, different responses of omnivores and herbivores to their shared host plants may influence the fitness, trophic identity, and population dynamics of the omnivores. 2. The aim of the present study was to show that an omnivorous heteropteran (Anthocoris nemorum L.) and two strictly herbivorous prey species respond differently to different genotypes of their shared host plant, Salix. Some plant genotypes were sub‐optimal for the omnivore, although suitable for the herbivores, and vice versa. 3. The contrasting patterns of plant suitability for the omnivore and the herbivores highlight an interaction between plant genotype and omnivores' access to animal food. Plant genotypes that were sub‐optimal for the omnivore when herbivores were experimentally excluded became the best host plants when herbivores were present, as in the latter situation additional prey became available. By contrast, the quality of plant genotypes that were intrinsically suitable for omnivores, did not improve when herbivores were present as these plant genotypes were intrinsically sub‐optimal for herbivores, thus providing omnivores with almost no additional animal food. 4. The differential responses of omnivores and their prey to the same host‐plant genotypes should allow omnivores to colonise sub‐optimal host plants in their capacity as predators, and to colonise more suitable host plants in their capacity as herbivores. It may thus be difficult for Salix to escape herbivory entirely, as it will rarely be unsuitable for both omnivores and pure herbivores at the same time.     

Allometric scaling of isokinetic peak torque: the Nebraska Wrestling Study

Allometric scaling has been used increasingly in the exercise sciences to control statistically for body size differences in physical performance variables. The purpose of this study was to use multivariate allometric scaling to examine the influence of fat-free mass (FFM) on age-related differences in strength in young club (8-13 years) and high-school (14-18 years) wrestlers. The dependent variables were log-transformed values of isokinetic peak torque for leg extension and flexion at 0.52, 3.14, and 5.24 rad x s(-1)(30, 180, and 300 x s(-1)). The independent variables used in the multiple regression analyses were log-transformed values for FFM, age, and the FFM versus age interaction. The resulting regression equations were of the form: log Y = log a + b1 log X1 + b2 log X2 + bn log Xn. The initial multiple regression analyses showed significant interaction effects (P < 0.05) for all dependent variables, therefore separate regression analyses were performed for the younger and older groups of wrestlers. The results indicate that for the younger wrestlers there were increases in isokinetic peak torque at all velocities across age after controlling for FFM. The FFM scaling exponents ranged from 0.94 to 1.31. All exponents included 1.0 in the 95% confidence interval, except for extension at 3.14 rad x s(-1). For the high-school wrestlers, both FFM and age were significant for the extension data, but only FFM was significant for the flexion data. All FFM exponents included 1.0 in the 95% confidence interval. These results indicate that the relationship between FFM and peak torque differed across age. In addition, with the exception of the flexion data for the high-school wrestlers, within each group increases in isokinetic peak torque occurred across age, independent of increases in FFM. The causes of the age effect for strength are speculative, but it may be due to developmental changes in neuromuscular function, alterations in the distribution of muscle mass as a percentage of FFM and/or the distribution of FFM across body segments.