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121.
ERIC A. HOBBIE JILLIAN GREGG† DAVID M. OLSZYK† PAUL T. RYGIEWICZ† DAVID T. TINGEY† 《Global Change Biology》2002,8(11):1072-1084
Models of photosynthesis, respiration, and export predict that foliar labile carbon (C) should increase with elevated CO2 but decrease with elevated temperature. Sugars, starch, and protein can be compared between treatments, but these compounds make up only a fraction of the total labile pool. Moreover, it is difficult to assess the turnover of labile carbon between years for evergreen foliage. Here, we combined changes in foliar Carea (C concentration on an areal basis) as needles aged with changes in foliar isotopic composition (δ13C) caused by inputs of 13C‐depleted CO2 to estimate labile and structural C in needles of different ages in a four‐year, closed‐chamber mesocosm experiment in which Douglas‐fir (Pseudotsuga menziesii (Mirb.) Franco) seedlings were exposed to elevated temperature (ambient + 3.5 °C) and CO2 (ambient + 179 ppm). Declines in δ 13C of needle cohorts as they aged indicated incorporation of newly fixed labile or structural carbon. The δ 13C calculations showed that new C was 41 ± 2% and 28 ± 3% of total needle carbon in second‐ and third‐year needles, respectively, with higher proportions of new C in elevated than ambient CO2 chambers (e.g. 42 ± 2% vs. 37 ± 6%, respectively, for second‐year needles). Relative to ambient CO2, elevated CO2 increased labile C in both first‐ and second‐year needles. Relative to ambient temperature, elevated temperature diminished labile C in second‐year needles but not in first‐year needles, perhaps because of differences in sink strength between the two needle age classes. We hypothesize that plant‐soil feedbacks on nitrogen supply contributed to higher photosynthetic rates under elevated temperatures that partly compensated for higher turnover rates of labile C. Strong positive correlations between labile C and sugar concentrations suggested that labile C was primarily determined by carbohydrates. Labile C was negatively correlated with concentrations of cellulose and protein. Elevated temperature increased foliar %C, possibly due to a shift of labile constituents from low %C carbohydrates to relatively high %C protein. Decreased sugar concentrations and increased nitrogen concentrations with elevated temperature were consistent with this explanation. Because foliar constituents that vary in isotopic signature also vary in concentrations with leaf age or environmental conditions, inferences of ci/ca values from δ 13C of bulk leaf tissue should be done cautiously. Tracing of 13C through foliar carbon pools may provide new insight into foliar C constituents and turnover. 相似文献
122.
ERIC MAURER 《The Plant journal : for cell and molecular biology》2003,35(1):35-44
1. The effects of resource availability during ontogeny on subsequent feeding performance were investigated in larvae of the small-mouthed salamander ( Ambystoma texanum ).
2. Salamander larvae were reared individually in either high or low prey density treatments for 7 weeks prior to intermediate prey density foraging trials. Larvae from the low prey density treatment were on average 35% smaller in body size than individuals from the high prey density treatment.
3. Resource availability during development influenced larval feeding rates and altered the relationship between body size and three feeding performance measures (attack rates, capture success and feeding rates). Feeding rates in predation trials were also positively correlated with growth rate early in the larval period (until the end of week 5).
4. These results suggest that the environment to which developing organisms are exposed can have significant effects on subsequent behaviour, and that small-mouthed salamander larvae may show state-dependent changes in feeding behaviour in response to differences in long-term feeding history. Additionally, differences in feeding performance may influence the probability of survival to the adult stage for organisms that utilize ephemeral habitats. 相似文献
2. Salamander larvae were reared individually in either high or low prey density treatments for 7 weeks prior to intermediate prey density foraging trials. Larvae from the low prey density treatment were on average 35% smaller in body size than individuals from the high prey density treatment.
3. Resource availability during development influenced larval feeding rates and altered the relationship between body size and three feeding performance measures (attack rates, capture success and feeding rates). Feeding rates in predation trials were also positively correlated with growth rate early in the larval period (until the end of week 5).
4. These results suggest that the environment to which developing organisms are exposed can have significant effects on subsequent behaviour, and that small-mouthed salamander larvae may show state-dependent changes in feeding behaviour in response to differences in long-term feeding history. Additionally, differences in feeding performance may influence the probability of survival to the adult stage for organisms that utilize ephemeral habitats. 相似文献
123.
MARCEL KLAASSEN BEREND HABEKOTTÉ PETER SCHINKELSHOEK ERIC STIENEN PIET VAN TIENEN 《Ibis》1994,136(2):197-204
Time budgets of free-living chicks of Arctic Terns Sterna paradisaea and Common Terns S. hirundo throughout development are presented with special reference to changes in time allocation when growth rate varies. Chicks of both species were inactive most of the time observed (87%). Time allocated to the different behaviours changed during development and was generally better correlated with body mass than age. Slower growing nestlings were brooded more and allocated more time to quiescence and less time to locomotion, preening, begging and attacking (the latter two significant only for the Arctic Tern). The energetic implications of variation in time budgets with age and growth rate were considered. Parental brooding resulted in an average energy saving of nearly 40% of an individual nestling's thermoregulatory costs. Whereas thermoregulatory costs remained nearly unchanged in Arctic Tern chicks, these were negatively correlated with growth rate in Common Terns. Tentatively, we estimated a 30% reduction in a nestling's total energy requirement for a 50% reduction in average growth rate for both species. 相似文献
124.
Detection and analysis of genetic variation in Hordeum spontaneum populations from Israel using RAPD markers 总被引:8,自引:0,他引:8
Randomly amplified polymorphic DNA (RAPD) markers were used to analyse genetic diversity within and between Hordeum spontaneum populations sampled from Israel. Nei's index of genetic differentiation was used to partition diversity into within and between population components. Fifty-seven per cent of the variation detected was partitioned within 10 H. spontaneum populations. Using principal component and multiple regression analysis, part of the variation detected between populations was seen to be associated with certain ecogeographical factors. Fifty-eight per cent of the distribution of the phenotypic frequencies of three RAPD phenotypes detected using a single primer in 20 H. spontaneum populations could be accounted for by four ecogeographical variables, suggesting adaptive variation at certain RAPD loci. 相似文献
125.
The Respiratory Costs of Nitrogen Fixation in Soyabean, Cowpea, and White Clover: I. NITROGEN FIXATION AND THE RESPIRATION OF THE NODULATED ROOT 总被引:1,自引:0,他引:1
Soyabean, cowpea, and white clover, inoculated with effectiverhizobia, were grown singly with a standard mineral nutritionand light regime in controlled environments until seed maturation(in soyabean and cowpea) or late vegetative growth (white clover).Day/night temperature regimes were 23/18, 30/24, and 20/15 °Cin soyabean, cowpea, and white clover, respectively. The respiratorylosses of CO2 from the nodulated root systems were studied inrelation to the concurrent rate of fixation of atmospheric nitrogen.Despite differences in development, the effectiveness of thesymbioses, and the temperature of growth, all three legumesexhibited similar respiratory losses from nodulated roots perunit of nitrogen fixed. During intense nitrogen fixation, theaverage respiratory losses for the three legumes varied between6·3 and 6·8 mg C mg1 N; within each species,the losses varied more widely at different stages of development.These respiratory burdens reflect the total cost to the plantof the nodule/nitrogen fixation syndrome including the subtendingroots. The results are discussed in relation to the respiratoryeffluxes from nodules and roots, and to biochemical investigationsof the costs of nitrogen fixation. 相似文献
126.
When pea seeds were imbibed in water without their seed coats,vital staining revealed that cells on the abaxial surface ofthe cotyledons were dead. No damage occurred on the surfaceof cotyledons when the seeds were imbibed intact, or beneaththe testa when only half of the testa was removed. Cell deathoccurred as a result of rapid water uptake within the first2 min of imbibition, since reducing the rate of imbibition insolutions of Carbowax 4000 lessened the damage. Cell death wasrestricted to the outer layers of the cotyledons; inner tissuesremained alive. These observations supported the hypothesisthat rapid early leakage during imbibition of dry embryos resultedfrom the death of cells caused by the physical disruption ofmembranes. Imbibition damage resulted in reduced respirationand germination, a decline in the rate of food reserve transferfrom the cotyledons to the growing axis, and a lower growthrate in the seedlings produced. Greater sensitivity of embryosto imbibition damage at low temperature, and similarities betweenfeatures of imbibition damage and chilling injury led to thesuggestion that so-called chilling injury is the result of imbibitiondamage rather than the effects of low temperature. 相似文献
127.
WALTER MAJAK RUTH E. McDIARMID THOMAS W. POWELL ALBERT L. VAN RYSWYK DARRYL G. STOUT RICHARD J. WILLIAMS RICHARD E. TUCKER 《Plant, cell & environment》1979,2(4):335-340
Abstract. The alkaloids of reed canarygrass respond to changes in soil moisture and nitrogen fertility. Gramine and hordenine levels increased under field conditions of moisture deprivation and this effect was enchanced when nitrogen fertilizer was applied. The level of 5-methoxy-N-methyltryptamine was affected mainly by the nitrogen amendment. Exceptional alkaloid levels in reed canarygrass are interpreted in relation to climatic and plant osmotic parameters. Improved TLC fluorescence scanning procedures are presented for quantifying reed canarygrass alkaloids. 相似文献
128.
A quantitative analysis of the 14C-labelled assimilate suppliedby the expanded leaves on the primary shoot to growing leaves,stem, lateral shoots (branches or stolons) and roots in redand white clover was conducted during vegetative growth. Stem growth of the primary shoot was inhibited in both cloversand utilized no energy resources. The growing leaves at theprimary shoot apex of white clover imported 4 per cent of theshoot's assimilate compared with 10 per cent in red clover.At the basal end of the primary shoot, the tap root of whiteclover imported 16 per cent of the shoot's assimilate comparedwith 22 per cent in red clover. Branches in red clover and stolonsin white clover were by far the largest sinks for primary shootassimilate, importing 39 per cent and 63 per cent of the labelledassimilate, respectively. Analyses of the translocation of assimilate from individualprimary shoot leaves demonstrated that in both clovers olderleaves exported more of their assimilate to branches or stolons,whereas younger leaves exported more of their assimilate toroots, and possibly in white clover, to growing leaves at thetip of the shoot. Of the labelled assimilate exported to branchesor stolons, each primary shoot leaf exported preferentiallyto the branch or stolon in its own axil, but in addition exportedsubstantial quantities of assimilate to all other axillary shoots,particularly those arising from basal axils where the subtendingleaf had died. Trifolium repens, Trifolium pratense, red clover, white clover, assimilate partitioning, perennation 相似文献
129.
Genetic variation of Calycophyllum spruceanum in the Peruvian Amazon Basin, revealed by amplified fragment length polymorphism (AFLP) analysis 总被引:3,自引:0,他引:3
J. R. RUSSELL J. C. WEBER A. BOOTH W. POWELL & C. SOTELO-MONTES† I. K. DAWSON 《Molecular ecology》1999,8(2):199-204
An understanding of the level, structure and origin of genetic variation within and among populations of tropical trees is essential for devising optimum management strategies for their sustainable utilization and conservation. Here, amplified fragment length polymorphism (AFLP) analysis was used to partition genetic variation within and among nine populations of the predominantly riverine tree, Calycophyllum spruceanum , sampled across a wide geographical range along river tributaries of the Peruvian Amazon Basin. Analysis of molecular variance ( AMOVA ) employed 65 AFLP markers and revealed most variation among individuals within populations (91%), although variation among populations was highly significant ( P < 0.001). Calculation of genetic distances and nested AMOVA indicated a degree of structuring among populations based on geographical proximity, although clustering did not depend on geographical distance alone. No firm evidence was obtained for unidirectional seed dispersal by water playing an important role in determining genetic structure over the geographical range sampled. Implications of data for optimising genetic management of the species are discussed and areas for further study identified. 相似文献
130.
Pea stem tissue (Pisum sativum L. var. Alaska) was homogenizedin a recently-developed cytoskeleton-stabilizing buffer, CSB,(Abeand Da vies, 1991) and homogenates electrophoresed and blottedon to membranes. Blots probed individually withantibodies toactin, alpha-tubulin, and beta-tubulin, revealed bands withapparent molecular weights of 42, 46, and 4850 kDa,respectively.Blots probed with all three antibodies simultaneously revealedall three bands which could be distinguished in thesame lane.Homogenates of mouse 3T3 cells yielded an actin band at about42 kDa, but both alpha- and beta-tubulin appeared atabout 50kDa and thus could not be distinguished on blots probed simultaneously.This triple-blotting technique was, therefore,suitablefor pea tissue, but not for mouse tissue. In pea tissue, sedimentabletubulin and actin were found maximally in the 4000 xg pelletand less in successive 15000 and l00000xg pellets. Both EGTAand Mg2+ which had been found earlier to beessential for stabilityof the actin cytoskeleton as revealed by fluorescence microscopy,were essential for co-sedimentation of actinand tubulin. Incontrast to the results with pea stems, only the actin componentof the cytoskeleton could be isolated from mouse 3T3 cells usingCSB. Pea tissue was homogenized in CSB without PTE and the resultingcytoskeletal pellets resuspended in actin- or tubulin-solubilizingbuffers with and without PTE. In the absence of PTE, the bufferssolubilized their appropriate cytoskeletal protein, but littleof the other protein, while in the presence of PTE both proteinswere quite effectively solubilized by both buffers. Incontrast,in CSB with or without PTE, both proteins remained in the sedimentablefraction. These results, taken together withother evidence,indicate that microtubules, as well as microfilaments are importantcomponents of the sedimentable cytoskeletonfraction of peasand that the membrane system is intimately involved in organizationof the cytoskeleton in peas. Key words: Actin, tubulin, membranes, detergent, Ca2+, Mg2+, cytoskeleton 相似文献